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The map label for this gene is miaB [H]

Identifier: 161523785

GI number: 161523785

Start: 662990

End: 664363

Strand: Reverse

Name: miaB [H]

Synonym: Bmul_0605

Alternate gene names: 161523785

Gene position: 664363-662990 (Counterclockwise)

Preceding gene: 161523786

Following gene: 161523784

Centisome position: 19.27

GC content: 64.92

Gene sequence:

>1374_bases
ATGACGAAGAAAGTTTACGTAAAGACCTTCGGCTGCCAGATGAACGAGTACGACTCGGACAAGATGGTGGACGTACTGAA
TGCGGCCGAAGGCCTCGAGAAGACCGACTCCCCGGAAGACGCGGACATCATCCTGTTCAACACCTGCTCGGTGCGCGAAA
AAGCGCAGGAGAAGGTGTTCTCCGATCTCGGCCGCGTGCGCGAGCTGAAGGAAGCGAAGCCCGATCTGCTGATCGGCGTC
GGCGGCTGCGTCGCGAGCCAGGAAGGCGCGTCGATCGTGTCGCGCGCGCCGTACGTCGACCTCGTGTTCGGCCCGCAAAC
GCTGCACCGCCTGCCGCAGATGATCGACGCACGCCGCACGAGCGGCCGCGCGCAGGTCGACATCACGTTCCCCGAAATCG
AGAAGTTCGATCACCTGCCGCCCGCGCGCGTCGAAGGGCCGACCGCGTTCGTGTCGATCATGGAAGGCTGCTCGAAGTAC
TGCAGCTACTGCGTCGTGCCGTACACGCGCGGCGACGAAGTCTCGCGCCCGCTCGACGACGTGCTGACCGAAGTCGCCGG
CCTCGCCGATCAGGGCGTGCGCGAAGTCACGCTGCTCGGCCAGAACGTGAACGCGTATCGCGGCGCGCTGACGGCCGGCT
CGTCGGAGGTCGCCGATTTCGCGACGCTGATCGAATACGTCGCCGACATTCCGGGCATCGAACGGATCCGCTACACCACG
TCGCATCCGAAGGAATTCACGCAGCGGCTGATCGACACGTACGCGAAGGTGCCGAAGCTCGTGAACCATCTGCACCTGCC
GGTGCAGCACGGCTCGGACCGCATCCTGATGGCGATGAAGCGCGGCTACACGGTGCTCGAATACAAGTCGGTGATCCGCA
AGCTGCGCGCGATCCGTCCCGACCTGTCGCTGTCGACCGACCTGATCGTCGGCTTCCCCGGCGAAACGGAAGAGGATTTC
GACAAGATGATGGCGCTCGTGCACGACATGCGCTACGACACGAGCTTCTCGTTCATCTACAGCCCGCGCCCCGGCACGCC
GGCCGCGAACCTGCACGACGACACGCCGCGCGACGTGAAGCTCAAGCGGCTGCAGCTCGTGCAGGCGACGATCGAGGAAA
ACGTCGCGCGCATCAGCCAGTCGATGGTCGGCAAGGTCGAGCGTATCCTCGTCGAAGGGCCGTCGCGCAAGGATCCGAAC
GAACTCGCGGGCCGCACCGAGAACAACCGCGTCGTGAACTTCCCGGCGCCGCTCGCCGCGCATCCGCGCCTGATCGGCCA
GATGATCGACGTGAAGATCAACCACGCGTATCCGCACTCGCTGCGCGGCGAACTCGTGCTCGTCAGCGACGACGCGAGCG
CAGCCACGCACTAG

Upstream 100 bases:

>100_bases
TGCGCCCCGCGCGACCTGCGCAACCGGGCGCCGCGACGCGCTGCCGTCCCGATTCACCTTTGACACGCCCCCAGGTCAAC
CACTGCGAACGGCGAACCCC

Downstream 100 bases:

>100_bases
GCCCGGTGCGCGGAAACGCATGCGTTTCCGCGCCGCCGGCCGCCAGCCCAACAGGAGCCCGACGCCACTTTGAAGCCCGC
TCAAGCACTGGAATTCACCG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPDLLIGV
GGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRTSGRAQVDITFPEIEKFDHLPPARVEGPTAFVSIMEGCSKY
CSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSSEVADFATLIEYVADIPGIERIRYTT
SHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPDLSLSTDLIVGFPGETEEDF
DKMMALVHDMRYDTSFSFIYSPRPGTPAANLHDDTPRDVKLKRLQLVQATIEENVARISQSMVGKVERILVEGPSRKDPN
ELAGRTENNRVVNFPAPLAAHPRLIGQMIDVKINHAYPHSLRGELVLVSDDASAATH

Sequences:

>Translated_457_residues
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPDLLIGV
GGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRTSGRAQVDITFPEIEKFDHLPPARVEGPTAFVSIMEGCSKY
CSYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSSEVADFATLIEYVADIPGIERIRYTT
SHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPDLSLSTDLIVGFPGETEEDF
DKMMALVHDMRYDTSFSFIYSPRPGTPAANLHDDTPRDVKLKRLQLVQATIEENVARISQSMVGKVERILVEGPSRKDPN
ELAGRTENNRVVNFPAPLAAHPRLIGQMIDVKINHAYPHSLRGELVLVSDDASAATH
>Mature_456_residues
TKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVFSDLGRVRELKEAKPDLLIGVG
GCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRTSGRAQVDITFPEIEKFDHLPPARVEGPTAFVSIMEGCSKYC
SYCVVPYTRGDEVSRPLDDVLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSSEVADFATLIEYVADIPGIERIRYTTS
HPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPDLSLSTDLIVGFPGETEEDFD
KMMALVHDMRYDTSFSFIYSPRPGTPAANLHDDTPRDVKLKRLQLVQATIEENVARISQSMVGKVERILVEGPSRKDPNE
LAGRTENNRVVNFPAPLAAHPRLIGQMIDVKINHAYPHSLRGELVLVSDDASAATH

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=35.7588357588358, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=35.7588357588358, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI93277076, Length=467, Percent_Identity=27.4089935760171, Blast_Score=173, Evalue=3e-43,
Organism=Escherichia coli, GI1786882, Length=451, Percent_Identity=61.4190687361419, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1787057, Length=461, Percent_Identity=29.5010845986985, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=35.3448275862069, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71996771, Length=304, Percent_Identity=28.9473684210526, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356207, Length=485, Percent_Identity=33.8144329896907, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=28.5403050108932, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50740; Mature: 50608

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVF
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRELKEAKPDLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRT
HHHHHHHHHHHCCCCEEEECCCHHCCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHC
SGRAQVDITFPEIEKFDHLPPARVEGPTAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD
CCCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSSEVADFATLIEYVADIPGIERIRYTT
HHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECC
SHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCC
DLSLSTDLIVGFPGETEEDFDKMMALVHDMRYDTSFSFIYSPRPGTPAANLHDDTPRDVK
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHH
LKRLQLVQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEECCCCCHH
HPRLIGQMIDVKINHAYPHSLRGELVLVSDDASAATH
CHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
TKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVF
CCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHH
SDLGRVRELKEAKPDLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRT
HHHHHHHHHHHCCCCEEEECCCHHCCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHHC
SGRAQVDITFPEIEKFDHLPPARVEGPTAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD
CCCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCHHH
VLTEVAGLADQGVREVTLLGQNVNAYRGALTAGSSEVADFATLIEYVADIPGIERIRYTT
HHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEECC
SHPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHCCCCHHHHHHHHHHHHHHCC
DLSLSTDLIVGFPGETEEDFDKMMALVHDMRYDTSFSFIYSPRPGTPAANLHDDTPRDVK
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHH
LKRLQLVQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCEEEECCCCCHH
HPRLIGQMIDVKINHAYPHSLRGELVLVSDDASAATH
CHHHHHHHHEEEEECCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA