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The map label for this gene is rmlA1 [C]

Identifier: 161523766

GI number: 161523766

Start: 640011

End: 640733

Strand: Reverse

Name: rmlA1 [C]

Synonym: Bmul_0586

Alternate gene names: 161523766

Gene position: 640733-640011 (Counterclockwise)

Preceding gene: 161523767

Following gene: 161523764

Centisome position: 18.58

GC content: 73.03

Gene sequence:

>723_bases
ATGAGCACCTCCCTGACCACGGCGATGATCTTCGCCGCCGGACGCGGCGAACGCATGCGCCCGCTGACCGACACCTGCCC
GAAACCGCTGCTCGAAGCGGGCGGCAAGCCGCTGATCGTCTGGCAGATCGAGCGGCTCGCGCAGGCCGGCATCGAGACGA
TCGTGATCAACCATGCATGGCTCGGCGCGCAGATCGAGCAGGCGCTCGGCGACGGCGCGCGCTGGGGCGTGCGGCTCGTG
TACTCGGCCGAAGGCGAAGCGCTCGAGACGGCCGGCGGCATCGCGCAGGCGCTGCCGCTGCTCGAGCGCGACGGCCGGGC
GACGGTGTTCGTCGCCGTCAGCGGCGACGTGTTCTGCGCGTTCGACTACCGCACGCTCGCGCCGCGCGCCGCGCGGATGG
CCGAACTCGACGCGCCCGCGCTGCACCTCGTGATGGTGCCGAACCCGCCGTTCCACCCGGCCGGCGACTTCGCGCTCGGC
GACGACGGGCGCCTGACGCTCGACGGCGCCGCGCGCCTCACATTCGGCAACATCGGGCTGTACGACACGCGGATGTTCCG
CGATCTCGCGCGCGGCACGCGCCGCGCGCTGACGCCGTACTACCGCGCGGCCATCGAGGCCGGCCGCGCGAGCGGCGAGC
TGTACGAGGGAATCTGGGAGAACGTCGGCACGCCCGCGCAGTTGCACGAACTCGACGCGCGGCTGCGCGCTGCGGGCCGC
TAA

Upstream 100 bases:

>100_bases
CGTCCGCTCGCGCCGTTCGCGAAGCTGCTCGACGCGCTCGAGGGCCGCACCGACGACGTCGCCTATACGTTCTGACCGCC
CGACCACCACGCTGCCGAAC

Downstream 100 bases:

>100_bases
GCAAACACGCGCGCGGCGCCGCGCTTCACCGCTTGCGCACGGCGGCGATCACGCCGCTTCGGCCGCCTCGTCGCCGGCGG
CCGCGCGCTGCTGCTGCAGC

Product: nucleotidyl transferase

Products: dTDPglucose; Diphosphate [C]

Alternate protein names: Nucleotidyltransferase Family Protein; Nucleotidyl Transferase Family Protein; Sugar-Phosphate Nucleotidyl Transferase; Nucleoside-Diphosphate-Sugar Pyrophosphorylase; Mannose-1-Phosphate Guanyltransferase; Nucleotidyltransferase; Nucleotidyl Transferase Superfamily; Nucleotidyl Transferase Protein; Mannose-1-Phosphate Guanyltransferase-Related Protein; Nucleotidyltransferase Protein; Mannose-1-Phosphate Guanylyltransferase; Transaldolase AB; ADP-Glucose Pyrophosphorylase; Mannose-1-Phosphate Guanyltransferase Protein; Sugar Phosphate Nucleotidyltransferase; Sugar-Phosphate Nucleotidyltransferase; Polysaccharide Biosynthesis Protein; Nucleotidyl Transferase Family; Glucose-1-Phosphate Nucleotidyltransferase; Phosphoglucomutase; Nucleotidyl Transferase Superfamily Protein

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSTSLTTAMIFAAGRGERMRPLTDTCPKPLLEAGGKPLIVWQIERLAQAGIETIVINHAWLGAQIEQALGDGARWGVRLV
YSAEGEALETAGGIAQALPLLERDGRATVFVAVSGDVFCAFDYRTLAPRAARMAELDAPALHLVMVPNPPFHPAGDFALG
DDGRLTLDGAARLTFGNIGLYDTRMFRDLARGTRRALTPYYRAAIEAGRASGELYEGIWENVGTPAQLHELDARLRAAGR

Sequences:

>Translated_240_residues
MSTSLTTAMIFAAGRGERMRPLTDTCPKPLLEAGGKPLIVWQIERLAQAGIETIVINHAWLGAQIEQALGDGARWGVRLV
YSAEGEALETAGGIAQALPLLERDGRATVFVAVSGDVFCAFDYRTLAPRAARMAELDAPALHLVMVPNPPFHPAGDFALG
DDGRLTLDGAARLTFGNIGLYDTRMFRDLARGTRRALTPYYRAAIEAGRASGELYEGIWENVGTPAQLHELDARLRAAGR
>Mature_239_residues
STSLTTAMIFAAGRGERMRPLTDTCPKPLLEAGGKPLIVWQIERLAQAGIETIVINHAWLGAQIEQALGDGARWGVRLVY
SAEGEALETAGGIAQALPLLERDGRATVFVAVSGDVFCAFDYRTLAPRAARMAELDAPALHLVMVPNPPFHPAGDFALGD
DGRLTLDGAARLTFGNIGLYDTRMFRDLARGTRRALTPYYRAAIEAGRASGELYEGIWENVGTPAQLHELDARLRAAGR

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11761621, Length=121, Percent_Identity=34.7107438016529, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI11761619, Length=121, Percent_Identity=34.7107438016529, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133931050, Length=121, Percent_Identity=40.495867768595, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320148, Length=120, Percent_Identity=37.5, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355443, Length=123, Percent_Identity=35.7723577235772, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24644084, Length=123, Percent_Identity=35.7723577235772, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.24 [C]

Molecular weight: Translated: 25871; Mature: 25740

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSLTTAMIFAAGRGERMRPLTDTCPKPLLEAGGKPLIVWQIERLAQAGIETIVINHAW
CCCCCEEEEEEECCCCCCCCCCHHHCCHHHHHCCCCEEEEEEHHHHHHCCCEEEEEECCH
LGAQIEQALGDGARWGVRLVYSAEGEALETAGGIAQALPLLERDGRATVFVAVSGDVFCA
HHHHHHHHHCCCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHCCCCEEEEEEECCCEEEE
FDYRTLAPRAARMAELDAPALHLVMVPNPPFHPAGDFALGDDGRLTLDGAARLTFGNIGL
ECCHHHCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEECCCCCEEECCCEEEEECCCCC
YDTRMFRDLARGTRRALTPYYRAAIEAGRASGELYEGIWENVGTPAQLHELDARLRAAGR
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STSLTTAMIFAAGRGERMRPLTDTCPKPLLEAGGKPLIVWQIERLAQAGIETIVINHAW
CCCCEEEEEEECCCCCCCCCCHHHCCHHHHHCCCCEEEEEEHHHHHHCCCEEEEEECCH
LGAQIEQALGDGARWGVRLVYSAEGEALETAGGIAQALPLLERDGRATVFVAVSGDVFCA
HHHHHHHHHCCCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHCCCCEEEEEEECCCEEEE
FDYRTLAPRAARMAELDAPALHLVMVPNPPFHPAGDFALGDDGRLTLDGAARLTFGNIGL
ECCHHHCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEECCCCCEEECCCEEEEECCCCC
YDTRMFRDLARGTRRALTPYYRAAIEAGRASGELYEGIWENVGTPAQLHELDARLRAAGR
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; D-Glucose 1-phosphate; H+ [C]

Specific reaction: dTTP + D-Glucose 1-phosphate + H+ --> dTDPglucose + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA