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The map label for this gene is aroA [H]

Identifier: 161509632

GI number: 161509632

Start: 1535337

End: 1536635

Strand: Reverse

Name: aroA [H]

Synonym: USA300HOU_1402

Alternate gene names: 161509632

Gene position: 1536635-1535337 (Counterclockwise)

Preceding gene: 161509633

Following gene: 161509631

Centisome position: 53.49

GC content: 35.72

Gene sequence:

>1299_bases
ATGGTAAATGAACAAATCATTGATATTTCAGGTCCGTTAAAGGGCGAAATAGAAGTGCCGGGCGATAAGTCAATGACACA
CCGTGCAATCATGTTGGCGTCGCTAGCTGAAGGTGTATCTACTATATATAAGCCACTACTTGGCGAAGATTGTCGTCGTA
CGATGGACATTTTCCGACTGTTAGGTGTAGAAATCAAAGAAGATGATGAAAAATTAGTTGTGACTTCCCCAGGATATCAA
TCTTTTAACACGCCACATCAAGTATTGTATACAGGTAATTCTGGTACGACAACACGATTATTGGCAGGTTTGTTAAGTGG
TTTAGGTATTGAAAGTGTTTTGTCTGGCGATGTTTCAATTGGTAAAAGGCCAATGGATCGTGTCTTGAGACCATTGAAAC
TTATGGATGCGAATATTGAAGGTATTGAAGATAATTATACACCATTAATTATTAAGCCATCTGTCATAAAAGGTATAAAT
TATCAAATGGAAGTTGCAAGTGCACAAGTAAAAAGTGCCATTTTATTTGCAAGTTTGTTTTCTAAGGAACCGACCATCAT
TAAAGAATTAGATGTAAGTCGAAATCATACTGAGACGATGTTCAAACATTTTAATATTCCAATTGAAGCAGAAGGGTTAT
CAATTAATACAACCCCTGAAGCAATTCGATACATTAAACCTGCAGATTTTCATGTTCCTGGCGATATTTCATCTGCAGCG
TTCTTTATTGTTGCAGCACTTATCACACCAGGAAGTGATGTAACAATTCATAATGTTGGAATCAATCCAACACGTTCAGG
TATTATTGATATTGTTGAAAAAATGGGCGGTAATATCCAACTTTTCAATCAAACAACTGGTGCTGAACCTACTGCTTCTA
TTCGTATTCAATACACACCAATGCTTCAACCAATAACAATCGAAGGAGAATTAGTTCCAAAAGCAATTGATGAACTGCCT
GTAATAGCATTACTTTGTACACAAGCAGTTGGCACGAGTACAATTAAAGATGCCGAGGAATTAAAAGTAAAAGAAACAAA
TAGAATTGATACAACGGCTGATATGTTAAACTTGTTAGGGTTTGAATTACAACCAACTAATGATGGATTGATTATTCATC
CGTCAGAATTTAAAACAAATGCAACAGTTGATAGTTTAACTGATCATCGAATAGGAATGATGCTTGCAGTTGCTTCTCTA
CTTTCAAGCGAGCCTGTCAAAATCAAACAATTTGATGCTGTAAATGTATCATTTCCAGGATTTTTACCAAAACTAAAGCT
TTTAGAAAATGAGGGATAA

Upstream 100 bases:

>100_bases
GATTAGACAATTTGGAGATATCGTTGTACAACATGTTGATCAACTAACATTACAACATGCATGTGAACAATTAAAAACAT
ATTTTAAGTAGGTGAATGAA

Downstream 100 bases:

>100_bases
TATAAAATGGAAGATATCTATAAATTAATAGACGATATCAATCTACAAAAACTAGAAAATTTAGACTCTCGTGTTAATGA
AGCAATAACTACTGACAACG

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYKPLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQ
SFNTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTTPEAIRYIKPADFHVPGDISSAA
FFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMGGNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELP
VIALLCTQAVGTSTIKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASL
LSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG

Sequences:

>Translated_432_residues
MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYKPLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQ
SFNTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTTPEAIRYIKPADFHVPGDISSAA
FFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMGGNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELP
VIALLCTQAVGTSTIKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASL
LSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG
>Mature_432_residues
MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYKPLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQ
SFNTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGIN
YQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTTPEAIRYIKPADFHVPGDISSAA
FFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMGGNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELP
VIALLCTQAVGTSTIKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASL
LSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1787137, Length=428, Percent_Identity=28.0373831775701, Blast_Score=124, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6320332, Length=453, Percent_Identity=26.9315673289183, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792 [H]

Pfam domain/function: PF00275 EPSP_synthase [H]

EC number: =2.5.1.19 [H]

Molecular weight: Translated: 47004; Mature: 47004

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00104 EPSP_SYNTHASE_1 ; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYKPLLGEDCRRTMDIFRL
CCCCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
LGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSI
HCCEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCCC
GKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGINYQMEVASAQVKSAILFASLF
CCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHCCCCEEEEHHHHHHHHHHHHHHHH
SKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTTPEAIRYIKPADFHVPGDISSAA
CCCCCEEECCCCCCCHHHHHHHHCCCCEEECCEEECCCHHHHHHCCCCCCCCCCCCHHHH
FFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMGGNIQLFNQTTGAEPTASIRIQYTP
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEC
MLQPITIEGELVPKAIDELPVIALLCTQAVGTSTIKDAEELKVKETNRIDTTADMLNLLG
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHEECCCCCCCHHHHHHHHHC
FELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASLLSSEPVKIKQFDAVNVSFPG
CEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEECCCC
FLPKLKLLENEG
CCCHHHHHCCCC
>Mature Secondary Structure
MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYKPLLGEDCRRTMDIFRL
CCCCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
LGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSI
HCCEEECCCCEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCCC
GKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGINYQMEVASAQVKSAILFASLF
CCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHCCCCEEEEHHHHHHHHHHHHHHHH
SKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTTPEAIRYIKPADFHVPGDISSAA
CCCCCEEECCCCCCCHHHHHHHHCCCCEEECCEEECCCHHHHHHCCCCCCCCCCCCHHHH
FFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMGGNIQLFNQTTGAEPTASIRIQYTP
HHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEC
MLQPITIEGELVPKAIDELPVIALLCTQAVGTSTIKDAEELKVKETNRIDTTADMLNLLG
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHEECCCCCCCHHHHHHHHHC
FELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASLLSSEPVKIKQFDAVNVSFPG
CEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEECCCC
FLPKLKLLENEG
CCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA