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The map label for this gene is crr

Identifier: 161509589

GI number: 161509589

Start: 1462271

End: 1462771

Strand: Reverse

Name: crr

Synonym: USA300HOU_1359

Alternate gene names: 161509589

Gene position: 1462771-1462271 (Counterclockwise)

Preceding gene: 161509590

Following gene: 161509588

Centisome position: 50.92

GC content: 33.93

Gene sequence:

>501_bases
ATGTTTAAAAAATTATTCGGAAAAGGCAAAGAAGTTCAGAAAGATATCGCAATTTATGCACCATTAACTGGAGAATTCGT
GAAAATTGAAGATATTCCAGATCCTGTATTCGCACAAAAAATGATGGGCGAAGGTTTTGGTATTAATCCAACTGAAGGAG
AAGTTGTGTCTCCAATTGCAGGACGTGTTGACAATGTCTTTCCAACTAAGCATGCGATTGGGTTAAAAGCAGATAACGGA
TTAGAATTATTAGTTCATATCGGTTTAGACACAGTTCAATTAGACGGTGAAGGATTTGAAGTGTTAGTGTCTAGTGGTGA
CGAAGTTAACGTCGGAGATCCATTAGTAAGATTCAACCTTGAATATATTAATAATAACGCTAAATCTGTAATTTCACCAA
TTATAATTACTAACACTGATCAAGCAGCTTCAATTAATATTTATGATGAAAATGCTGTGATTAAAGGTGAAACAAAAGTG
ATTGATGTGACAATGAACTAA

Upstream 100 bases:

>100_bases
GTATCAATTCCGCTGCAATTCAATTTATTCCATATGAAAAGTTAGAAGAATTGGGTTATGGCGATTTAATATCACATTTT
GATAAGTAGGAGTGGAAAGT

Downstream 100 bases:

>100_bases
TGAAAAACTATTCGTTTTATCAATTTGTCATGACAGTTCGTGGTCGACACGACGATAAAGGTCGTCTAGCAGAAGAGATA
TTTGACGATCTTGCTTTCCC

Product: PTS family glucose/glucoside (glc) porter component IIA

Products: Protein Histidine; Sugar Phosphate.; glucose-6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGEGFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNG
LELLVHIGLDTVQLDGEGFEVLVSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
IDVTMN

Sequences:

>Translated_166_residues
MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGEGFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNG
LELLVHIGLDTVQLDGEGFEVLVSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
IDVTMN
>Mature_166_residues
MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGEGFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNG
LELLVHIGLDTVQLDGEGFEVLVSSGDEVNVGDPLVRFNLEYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
IDVTMN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain

Homologues:

Organism=Escherichia coli, GI1788757, Length=162, Percent_Identity=41.9753086419753, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1786894, Length=146, Percent_Identity=38.3561643835616, Blast_Score=128, Evalue=2e-31,
Organism=Escherichia coli, GI1790159, Length=141, Percent_Identity=39.7163120567376, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PTGA_STAAC (Q5HFZ9)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186308.1
- ProteinModelPortal:   Q5HFZ9
- SMR:   Q5HFZ9
- STRING:   Q5HFZ9
- EnsemblBacteria:   EBSTAT00000009503
- GeneID:   3238636
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1457
- TIGR:   SACOL1457
- eggNOG:   COG2190
- GeneTree:   EBGT00050000023800
- HOGENOM:   HBG517865
- OMA:   RRLISGQ
- ProtClustDB:   CLSK885342
- BioCyc:   SAUR93062:SACOL1457-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- InterPro:   IPR011055
- InterPro:   IPR001127
- TIGRFAMs:   TIGR00830

Pfam domain/function: PF00358 PTS_EIIA_1; SSF51261 Dup_hybrid_motif

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 17961; Mature: 17961

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS51093 PTS_EIIA_TYPE_1; PS00371 PTS_EIIA_TYPE_1_HIS

Important sites: ACT_SITE 86-86

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGEGFGINPTEGEVVSPIA
CCHHHHCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCEECCHH
GRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVLVSSGDEVNVGDPLVRFNL
CCCCCCCCCCEEEEEECCCCEEEEEEECCEEEEECCCCEEEEEECCCEECCCCCEEEEEE
EYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKVIDVTMN
EECCCCCHHHCCEEEEECCCCCEEEEEECCCEEEECCCEEEEEEEC
>Mature Secondary Structure
MFKKLFGKGKEVQKDIAIYAPLTGEFVKIEDIPDPVFAQKMMGEGFGINPTEGEVVSPIA
CCHHHHCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCEECCHH
GRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVLVSSGDEVNVGDPLVRFNL
CCCCCCCCCCEEEEEECCCCEEEEEEECCEEEEECCCCEEEEEECCCEECCCCCEEEEEE
EYINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKVIDVTMN
EECCCCCHHHCCEEEEECCCCCEEEEEECCCEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar; phosphoenolpyruvate; beta-D-glucose [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm] [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytop

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA