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The map label for this gene is odhB [H]

Identifier: 161509577

GI number: 161509577

Start: 1449841

End: 1451109

Strand: Reverse

Name: odhB [H]

Synonym: USA300HOU_1347

Alternate gene names: 161509577

Gene position: 1451109-1449841 (Counterclockwise)

Preceding gene: 161509578

Following gene: 161509576

Centisome position: 50.51

GC content: 34.04

Gene sequence:

>1269_bases
ATGCCAGAGGTTAAAGTTCCAGAATTAGCAGAATCTATTACAGAAGGTACCATTGCAGAATGGTTGAAAAACGTAGGGGA
TAGCGTAGAAAAAGGTGAAGCTATTCTTGAATTAGAAACTGATAAAGTTAATGTCGAAGTTGTATCTGAAGAAGCAGGTG
TATTATCTGAACAACTTGCAAGTGAAGGCGACACTGTAGAAGTTGGACAAGCAATTGCTATCATCGGCGAAGGTAGTGGC
AATGCTTCTAAAGAAAATAGTAACGACAATACTCCACAACAAAATGAAGAAACAAATAATAAAAAAGAAGAAACAACAAA
TAATTCGGTAGATAAAGCTGAAGTAAATCAAGCAAATGATGACAATCAGCAACGTATTAATGCTACGCCTTCTGCGCGTC
GATATGCTCGTGAAAATGGTGTGAATCTTGCTGAAGTAAGTCCGAAAACAAATGATGTGGTTCGTAAAGAAGATATTGAT
AAGAAACAACAGGCACCGGCATCAACACAAACAACACAACAAGCATCTGCAAAAGAAGAGAAAAAATACAATCAATATCC
TACAAAACCAGTGATTCGTGAAAAAATGTCACGTAGAAAGAAAACAGCTGCCAAAAAATTATTAGAGGTATCTAATAATA
CAGCTATGTTAACAACATTTAACGAAGTTGACATGACAAATGTTATGGAATTGCGTAAACGTAAGAAAGAACAATTTATG
AAAGATCATGATGGTACTAAATTAGGATTTATGTCATTCTTTACTAAAGCTTCTGTAGCAGCTTTGAAAAAGTATCCAGA
AGTTAATGCAGAAATCGACGGCGACGACATGATTACGAAACAATATTATGATATTGGTGTAGCTGTTTCTACAGATGATG
GATTATTAGTACCATTTGTAAGAGATTGTGATAAAAAGAATTTTGCAGAAATCGAAGCAGAAATTGCTAATTTAGCAGTT
AAAGCACGAGAGAAAAAACTTGGCTTAGATGATATGGTTAATGGTTCATTTACGATTACAAATGGCGGTATTTTTGGATC
AATGATGAGTACGCCAATTATCAATGGTAATCAAGCTGCAATCTTAGGCATGCATTCAATTATTACAAGACCAATTGCGA
TTGATCAAGATACAATCGAAAATCGTCCAATGATGTATATTGCATTAAGCTATGATCATAGAATTATTGACGGTAAAGAA
GCAGTTGGATTCTTAAAAACAATTAAAGAATTAATTGAAAACCCAGAAGACTTATTATTAGAATCTTAA

Upstream 100 bases:

>100_bases
CAAAGGGCTGCTCCAGCTGAAGGCGATGGAGAAATTCATAAACTTGTTCAAAATAAAATTATAGAAAATGCATTAAAAAA
TAACTAGGGGGAAATAAGTC

Downstream 100 bases:

>100_bases
TCCACAACACAAAATAGTTTACTATTCCTAAAAGCGGGATTAAATCAATAACAAACAGCAGTAAGATTATTTCCTAGTCG
AAATATCTTACTGCTGTACT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSG
NASKENSNDNTPQQNEETNNKKEETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFM
KDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAV
KAREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKE
AVGFLKTIKELIENPEDLLLES

Sequences:

>Translated_422_residues
MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSG
NASKENSNDNTPQQNEETNNKKEETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
KKQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFM
KDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAV
KAREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKE
AVGFLKTIKELIENPEDLLLES
>Mature_421_residues
PEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGN
ASKENSNDNTPQQNEETNNKKEETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDIDK
KQQAPASTQTTQQASAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFMK
DHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVK
AREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKEA
VGFLKTIKELIENPEDLLLES

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=54.1125541125541, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=26.6666666666667, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=26.6666666666667, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI110671329, Length=435, Percent_Identity=26.4367816091954, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=27.5862068965517, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI260898739, Length=163, Percent_Identity=35.5828220858896, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786946, Length=420, Percent_Identity=43.8095238095238, Blast_Score=331, Evalue=5e-92,
Organism=Escherichia coli, GI1786305, Length=434, Percent_Identity=29.9539170506912, Blast_Score=161, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=421, Percent_Identity=39.1923990498812, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=28.5714285714286, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=26.9585253456221, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=201, Percent_Identity=32.8358208955224, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320352, Length=418, Percent_Identity=40.1913875598086, Blast_Score=299, Evalue=5e-82,
Organism=Saccharomyces cerevisiae, GI6324258, Length=463, Percent_Identity=26.3498920086393, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=54.2600896860987, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=27.972027972028, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI20129315, Length=448, Percent_Identity=24.7767857142857, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24582497, Length=431, Percent_Identity=24.5939675174014, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 46674; Mature: 46543

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLA
CCCCCCHHHHHHHHCCHHHHHHHHHCCHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHH
SEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKKEETTNNSVDKAEVNQAND
CCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHCCCCCC
DNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDIDKKQQAPASTQTTQQASAKEE
CCHHHCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHCCCHHHCCCCCHHHHHHHHHHHH
KKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFM
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
KDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFV
HHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEEEECCCCEEEHHH
RDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCEEHHHHHCCCCCCCCEE
ILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKEAVGFLKTIKELIENPEDLLL
EEEHHHHHHCCCCCCCHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHHC
ES
CC
>Mature Secondary Structure 
PEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVNVEVVSEEAGVLSEQLA
CCCCCHHHHHHHHCCHHHHHHHHHCCHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHH
SEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKKEETTNNSVDKAEVNQAND
CCCCEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHCCCCCC
DNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDIDKKQQAPASTQTTQQASAKEE
CCHHHCCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHCCCHHHCCCCCHHHHHHHHHHHH
KKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLTTFNEVDMTNVMELRKRKKEQFM
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
KDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDDMITKQYYDIGVAVSTDDGLLVPFV
HHCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEEEEEECCCCEEEHHH
RDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVNGSFTITNGGIFGSMMSTPIINGNQAA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCEEHHHHHCCCCCCCCEE
ILGMHSIITRPIAIDQDTIENRPMMYIALSYDHRIIDGKEAVGFLKTIKELIENPEDLLL
EEEHHHHHHCCCCCCCHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHHHC
ES
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA