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The map label for this gene is bbmA [H]

Identifier: 160902721

GI number: 160902721

Start: 1354836

End: 1356227

Strand: Direct

Name: bbmA [H]

Synonym: Pmob_1265

Alternate gene names: 160902721

Gene position: 1354836-1356227 (Clockwise)

Preceding gene: 160902720

Following gene: 160902723

Centisome position: 62.45

GC content: 30.24

Gene sequence:

>1392_bases
ATGAGATCAGAAATTGGATATGAAATATTTGTAGATAGATTTTTTTCCTCTCAGGAAAAAAATCATACAATACCTTGGGA
TAGTAAAATTAAGGGACAGGAAAAACAAGAATACGATTTTTATGGGGGCGACCTTTTAGGGATTACTGAAAAAATAGACT
ATCTTTACGACCTTGGAATAGATTTTATTTACTTAACTCCAATATTCGAAGCAAAAACAAATCATAGATATGATTGTACG
AATTATTTTAGAATCGATCCCTTAATTGGAAATGAACAAAACCTAGAACTTTTATGTAAAAATCTTGCCCAAAAGAATAT
TAAATTATTCTTAGACATTGCTCTAAATCACATGGGTTCTGATAGTATATGGTTTCAAAAAGCTAAGGCAAATAATAATG
AACATAATTACTTTCGAATAAAAGGCGGAGAATTCACCCACTGGGCAAATGTTGAAAAATTGGTAGAACTCAACTTAGAA
AATTTAGAATTGCAATCCATCCTCTGGGACGGAAATAATTCTGCTATGAAATACTGGAGTAAGTTTGGAATTGCAGGTTG
GAGATTAGATTGTGCTTATGAATTGGGTCAAGATATTTTAAATAAAATATATACTTCTTTAAAAGAATCATTTCATATTT
CTCTCATAGGAGAAATATGGTCTTATCCCGAAAAATGGATTCAAGAAGGCGTTTTAGATGGAATAATGAATTATTATTAT
AAAGATCTTATTGATAACATTATTGACCAGAAAATTGATGGTACATTATTTACCCAAATTGTTGAAAAGCTCTATGTTTC
TTGTGGGAACAAACTCCTAAAAAGTTGGAACATTTTGTCATCTCATGATACTTCTAGAATAAGAACAAGATATGGCAAAA
ACTGGAAAATTGCAGTTGCATTGCAATTCATGCTTCCGGGAAGTCCATTAATATATTACGGAGAAGAAATAGGCTTAGAG
GCAGAAGGAGATCCTTATTGTAGAACTCCTATGAAATGGAATTCTTGGTCTGAAGAGAATTATGAATCCTATAATTTTTA
CAAAAAAATGATCAAATTTTTTGAAAACTCAAAAGCATTACAAGAAGGATCGCTAAAGGCTGTTTTTTCTAACAATGAGA
ATATTGTTTCTTTTTTGAGAACAACCGACAGTATAGAAGACCTTAGACTTGTTATTGCCAATCCTACTTCACAACGCCAA
AATTATCTTTTATATACTCAAGAATCTTCTCTTATGAATAAAACAATCTTAAAAGATTGTTTTAGTAACAATATTGCACA
AGTTGAGAATTCTAAAATTATTGGTCAAATTTCTCCAAATTCATTTTCTATATTTATGCCACATATCGAAAGAGAAACTT
CAAAATACACTCCTTACAAAAGGATCTATTAA

Upstream 100 bases:

>100_bases
TTTGTGCTGCATCAATTATTGGTTCACTACCAATAATGATAATTTTTCTATTGCTTCAAAAATATATTGCCAAAGGTTTA
ACTCAAGGAGCTGTTAAGGA

Downstream 100 bases:

>100_bases
AAAGAATTAAGGTAGATAATTAATTTTACTGAAATATCTAACTTTATTTTCAAATAATGTACTAAGTAATTTTTCCCTAC
TATTGATTAATAATGCATTA

Product: alpha amylase catalytic subunit

Products: alpha-D-glucose; beta-D-glucose; amylose(n-1) [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRSEIGYEIFVDRFFSSQEKNHTIPWDSKIKGQEKQEYDFYGGDLLGITEKIDYLYDLGIDFIYLTPIFEAKTNHRYDCT
NYFRIDPLIGNEQNLELLCKNLAQKNIKLFLDIALNHMGSDSIWFQKAKANNNEHNYFRIKGGEFTHWANVEKLVELNLE
NLELQSILWDGNNSAMKYWSKFGIAGWRLDCAYELGQDILNKIYTSLKESFHISLIGEIWSYPEKWIQEGVLDGIMNYYY
KDLIDNIIDQKIDGTLFTQIVEKLYVSCGNKLLKSWNILSSHDTSRIRTRYGKNWKIAVALQFMLPGSPLIYYGEEIGLE
AEGDPYCRTPMKWNSWSEENYESYNFYKKMIKFFENSKALQEGSLKAVFSNNENIVSFLRTTDSIEDLRLVIANPTSQRQ
NYLLYTQESSLMNKTILKDCFSNNIAQVENSKIIGQISPNSFSIFMPHIERETSKYTPYKRIY

Sequences:

>Translated_463_residues
MRSEIGYEIFVDRFFSSQEKNHTIPWDSKIKGQEKQEYDFYGGDLLGITEKIDYLYDLGIDFIYLTPIFEAKTNHRYDCT
NYFRIDPLIGNEQNLELLCKNLAQKNIKLFLDIALNHMGSDSIWFQKAKANNNEHNYFRIKGGEFTHWANVEKLVELNLE
NLELQSILWDGNNSAMKYWSKFGIAGWRLDCAYELGQDILNKIYTSLKESFHISLIGEIWSYPEKWIQEGVLDGIMNYYY
KDLIDNIIDQKIDGTLFTQIVEKLYVSCGNKLLKSWNILSSHDTSRIRTRYGKNWKIAVALQFMLPGSPLIYYGEEIGLE
AEGDPYCRTPMKWNSWSEENYESYNFYKKMIKFFENSKALQEGSLKAVFSNNENIVSFLRTTDSIEDLRLVIANPTSQRQ
NYLLYTQESSLMNKTILKDCFSNNIAQVENSKIIGQISPNSFSIFMPHIERETSKYTPYKRIY
>Mature_463_residues
MRSEIGYEIFVDRFFSSQEKNHTIPWDSKIKGQEKQEYDFYGGDLLGITEKIDYLYDLGIDFIYLTPIFEAKTNHRYDCT
NYFRIDPLIGNEQNLELLCKNLAQKNIKLFLDIALNHMGSDSIWFQKAKANNNEHNYFRIKGGEFTHWANVEKLVELNLE
NLELQSILWDGNNSAMKYWSKFGIAGWRLDCAYELGQDILNKIYTSLKESFHISLIGEIWSYPEKWIQEGVLDGIMNYYY
KDLIDNIIDQKIDGTLFTQIVEKLYVSCGNKLLKSWNILSSHDTSRIRTRYGKNWKIAVALQFMLPGSPLIYYGEEIGLE
AEGDPYCRTPMKWNSWSEENYESYNFYKKMIKFFENSKALQEGSLKAVFSNNENIVSFLRTTDSIEDLRLVIANPTSQRQ
NYLLYTQESSLMNKTILKDCFSNNIAQVENSKIIGQISPNSFSIFMPHIERETSKYTPYKRIY

Specific function: Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferrin

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. BbmA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=440, Percent_Identity=28.8636363636364, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1790687, Length=346, Percent_Identity=24.8554913294798, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32565753, Length=320, Percent_Identity=20.625, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322245, Length=366, Percent_Identity=21.0382513661202, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=184, Percent_Identity=24.4565217391304, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321731, Length=184, Percent_Identity=24.4565217391304, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324416, Length=189, Percent_Identity=24.8677248677249, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322241, Length=189, Percent_Identity=24.8677248677249, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322021, Length=189, Percent_Identity=24.8677248677249, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321726, Length=189, Percent_Identity=24.8677248677249, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586593, Length=194, Percent_Identity=26.8041237113402, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45549022, Length=426, Percent_Identity=21.830985915493, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24586589, Length=190, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24586587, Length=183, Percent_Identity=24.5901639344262, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI221330053, Length=87, Percent_Identity=35.632183908046, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586591, Length=183, Percent_Identity=23.4972677595628, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586599, Length=87, Percent_Identity=37.9310344827586, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586597, Length=180, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: 3.2.1.20 [C]

Molecular weight: Translated: 54279; Mature: 54279

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00334 MYB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSEIGYEIFVDRFFSSQEKNHTIPWDSKIKGQEKQEYDFYGGDLLGITEKIDYLYDLGI
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCC
DFIYLTPIFEAKTNHRYDCTNYFRIDPLIGNEQNLELLCKNLAQKNIKLFLDIALNHMGS
CEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHCCC
DSIWFQKAKANNNEHNYFRIKGGEFTHWANVEKLVELNLENLELQSILWDGNNSAMKYWS
CCCEEEECCCCCCCCCEEEEECCCCCCHHCHHHHHHCCCCCEEEEEEEECCCCHHHHHHH
KFGIAGWRLDCAYELGQDILNKIYTSLKESFHISLIGEIWSYPEKWIQEGVLDGIMNYYY
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHCCHHHHHHHHHHHHHHHHHH
KDLIDNIIDQKIDGTLFTQIVEKLYVSCGNKLLKSWNILSSHDTSRIRTRYGKNWKIAVA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEE
LQFMLPGSPLIYYGEEIGLEAEGDPYCRTPMKWNSWSEENYESYNFYKKMIKFFENSKAL
EEEECCCCCEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
QEGSLKAVFSNNENIVSFLRTTDSIEDLRLVIANPTSQRQNYLLYTQESSLMNKTILKDC
HCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHH
FSNNIAQVENSKIIGQISPNSFSIFMPHIERETSKYTPYKRIY
HCCCCEEECCCEEEEEECCCCEEEEECCHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRSEIGYEIFVDRFFSSQEKNHTIPWDSKIKGQEKQEYDFYGGDLLGITEKIDYLYDLGI
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCC
DFIYLTPIFEAKTNHRYDCTNYFRIDPLIGNEQNLELLCKNLAQKNIKLFLDIALNHMGS
CEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHCCC
DSIWFQKAKANNNEHNYFRIKGGEFTHWANVEKLVELNLENLELQSILWDGNNSAMKYWS
CCCEEEECCCCCCCCCEEEEECCCCCCHHCHHHHHHCCCCCEEEEEEEECCCCHHHHHHH
KFGIAGWRLDCAYELGQDILNKIYTSLKESFHISLIGEIWSYPEKWIQEGVLDGIMNYYY
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHCCHHHHHHHHHHHHHHHHHH
KDLIDNIIDQKIDGTLFTQIVEKLYVSCGNKLLKSWNILSSHDTSRIRTRYGKNWKIAVA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEE
LQFMLPGSPLIYYGEEIGLEAEGDPYCRTPMKWNSWSEENYESYNFYKKMIKFFENSKAL
EEEECCCCCEEEECHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
QEGSLKAVFSNNENIVSFLRTTDSIEDLRLVIANPTSQRQNYLLYTQESSLMNKTILKDC
HCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHH
FSNNIAQVENSKIIGQISPNSFSIFMPHIERETSKYTPYKRIY
HCCCCEEECCCEEEEEECCCCEEEEECCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): {} [C]

Substrates: Maltose; H2O; 1,4-alpha-D-glucan [C]

Specific reaction: Maltose + H2O = 2 alpha-D-glucose H2O + 1,4-alpha-D-glucan = beta-D-glucose + amylose(n-1) [C]

General reaction: O-Glycosyl bond hydrolysis; Exohydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]