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The map label for this gene is pmbA [H]

Identifier: 160902625

GI number: 160902625

Start: 1249401

End: 1250735

Strand: Direct

Name: pmbA [H]

Synonym: Pmob_1165

Alternate gene names: 160902625

Gene position: 1249401-1250735 (Clockwise)

Preceding gene: 160902624

Following gene: 160902626

Centisome position: 57.59

GC content: 36.48

Gene sequence:

>1335_bases
ATGACTAATATAAAATCAATTATAGAAAAATCCATGAAAGAGATAAAAAGTAAAGGGCTTAAAGGACAGATAAATGTGAT
TTCCACTGAAAGTAAGAATGCCTCTTTCAGTAATGGAAAGTTAGAACAGTTAACCGAAGGAGAAAAGGGAGCAGCAGGTA
TCAAAGTTATTTCTCCCGATGGAAGAACAGCCACATCGTCATCTAATATTTTAACAGAAGAAGGAATAATGCAAGCTGTT
GAAAAAGCAATTGAAATGACTAAATATACAGAAAAAGACGAAGCAAACGACATATCAAATGACGAACAGTTTACATACAT
AGATTGGGCTTTCGATGCAGACACAAAGGATATGAATTTAGACGAGGTTATGAAATTAGCTCAAGAACTAGAAAAAAAGG
CTAAGAGTGAAGATGAAAGGATAAAATTTGTTCGTGGTGCTGAGTATGAAGTTGCATTAACCAGGATTCATTTTGGCAAT
ACGAATGGTCTTTACAAAAACGCTTTGTTTACAAACGCTGCCAGTTCGATTAGTTTAGCAGCAATTGAAGGAGAAGATTC
CTCATTTGGTTTTGATTATCAATTATCTCAAAGCTACAGGCTTATAGACATAGACCGCATAGTGAAAAATTCTGTAGAAA
TGGCGGTATCAGGTTTAAAATCAGAGGTTTTAAGTTCTGGTAGATACGATATTGTCATGAGTCCTTTCGCTTCTAGTATG
TTCCTAGGGACATTGATAACTCCTTTGTCGGGAGAAAATGTTTACAAGGGTAAATCTTTTTTGAAAGATGAATTAGGTGA
GAAAGTAGCCAATTCAACGGTTACCCTCATACATGATCCTATGAATGGTTCTGCACCAATCATAGCCTCCTTTGACAATG
AAGGAACCAATACCTCAAGATTCAACGTTATAGAAAATGGTGTCCTAAAAGGGTTCTTACATAATATATATTCTGCAAAA
AAGTTGGGTGCAAAACCAACCGGCAACAGTTTTGCATCGGTGGAATCTCCAAATCCTTCCATTGGAACGATAAACCTTCA
TTTTATCGCTAACAAGACGAGAAAAGATATTTTGAATGTTCCAAAAGCTCTTTATGTCACCAACATAATGGGTATGCATA
CCGCCGATCCGGTTTCTGGAAGATTCTCTGTGCAAATAAGTGGAAGATTAATTGAAAACGGAGAGTTTGTAAGAAGTTTT
AGAGGGATGACCTTAGCTGGAACGCTGGAAGAGTTGTTGAATAATTTAGAATATATAGGTTCTGATTTCAAGTATTTAGG
TCCAGTAGCCGGTTCTACTATGTTGATAAGGGATTTAAGCGTGGGCGGTAAATAG

Upstream 100 bases:

>100_bases
GTGGTAAAGACGGTCAAGGGGCTCCAGTTTCCGATGCCCAGCCTACCATAAGAATACCGAAGTTAATTGTTGGTGGAATA
GTAAAAGGGGGTAACAATTA

Downstream 100 bases:

>100_bases
AAGAAGAAGTCTAAAAAAGGAGTATAAGGCGTTTAATCGCTTTTAAAAGCGTTTAAACGCCTTTTTTTCGTCTTAATTGG
GAATTATCTAGTTTGAGAAA

Product: peptidase U62 modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTNIKSIIEKSMKEIKSKGLKGQINVISTESKNASFSNGKLEQLTEGEKGAAGIKVISPDGRTATSSSNILTEEGIMQAV
EKAIEMTKYTEKDEANDISNDEQFTYIDWAFDADTKDMNLDEVMKLAQELEKKAKSEDERIKFVRGAEYEVALTRIHFGN
TNGLYKNALFTNAASSISLAAIEGEDSSFGFDYQLSQSYRLIDIDRIVKNSVEMAVSGLKSEVLSSGRYDIVMSPFASSM
FLGTLITPLSGENVYKGKSFLKDELGEKVANSTVTLIHDPMNGSAPIIASFDNEGTNTSRFNVIENGVLKGFLHNIYSAK
KLGAKPTGNSFASVESPNPSIGTINLHFIANKTRKDILNVPKALYVTNIMGMHTADPVSGRFSVQISGRLIENGEFVRSF
RGMTLAGTLEELLNNLEYIGSDFKYLGPVAGSTMLIRDLSVGGK

Sequences:

>Translated_444_residues
MTNIKSIIEKSMKEIKSKGLKGQINVISTESKNASFSNGKLEQLTEGEKGAAGIKVISPDGRTATSSSNILTEEGIMQAV
EKAIEMTKYTEKDEANDISNDEQFTYIDWAFDADTKDMNLDEVMKLAQELEKKAKSEDERIKFVRGAEYEVALTRIHFGN
TNGLYKNALFTNAASSISLAAIEGEDSSFGFDYQLSQSYRLIDIDRIVKNSVEMAVSGLKSEVLSSGRYDIVMSPFASSM
FLGTLITPLSGENVYKGKSFLKDELGEKVANSTVTLIHDPMNGSAPIIASFDNEGTNTSRFNVIENGVLKGFLHNIYSAK
KLGAKPTGNSFASVESPNPSIGTINLHFIANKTRKDILNVPKALYVTNIMGMHTADPVSGRFSVQISGRLIENGEFVRSF
RGMTLAGTLEELLNNLEYIGSDFKYLGPVAGSTMLIRDLSVGGK
>Mature_443_residues
TNIKSIIEKSMKEIKSKGLKGQINVISTESKNASFSNGKLEQLTEGEKGAAGIKVISPDGRTATSSSNILTEEGIMQAVE
KAIEMTKYTEKDEANDISNDEQFTYIDWAFDADTKDMNLDEVMKLAQELEKKAKSEDERIKFVRGAEYEVALTRIHFGNT
NGLYKNALFTNAASSISLAAIEGEDSSFGFDYQLSQSYRLIDIDRIVKNSVEMAVSGLKSEVLSSGRYDIVMSPFASSMF
LGTLITPLSGENVYKGKSFLKDELGEKVANSTVTLIHDPMNGSAPIIASFDNEGTNTSRFNVIENGVLKGFLHNIYSAKK
LGAKPTGNSFASVESPNPSIGTINLHFIANKTRKDILNVPKALYVTNIMGMHTADPVSGRFSVQISGRLIENGEFVRSFR
GMTLAGTLEELLNNLEYIGSDFKYLGPVAGSTMLIRDLSVGGK

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=441, Percent_Identity=24.9433106575964, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789640, Length=229, Percent_Identity=29.2576419213974, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48445; Mature: 48314

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIKSIIEKSMKEIKSKGLKGQINVISTESKNASFSNGKLEQLTEGEKGAAGIKVISPD
CCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCEEEECCC
GRTATSSSNILTEEGIMQAVEKAIEMTKYTEKDEANDISNDEQFTYIDWAFDADTKDMNL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCH
DEVMKLAQELEKKAKSEDERIKFVRGAEYEVALTRIHFGNTNGLYKNALFTNAASSISLA
HHHHHHHHHHHHHHCCCCHHEEEEECCCEEEEEEEEEECCCCCCCHHHHHHCCCCCEEEE
AIEGEDSSFGFDYQLSQSYRLIDIDRIVKNSVEMAVSGLKSEVLSSGRYDIVMSPFASSM
EEECCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
FLGTLITPLSGENVYKGKSFLKDELGEKVANSTVTLIHDPMNGSAPIIASFDNEGTNTSR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCCCCCCH
FNVIENGVLKGFLHNIYSAKKLGAKPTGNSFASVESPNPSIGTINLHFIANKTRKDILNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCEEEEEEEEEECCHHHHHHCC
PKALYVTNIMGMHTADPVSGRFSVQISGRLIENGEFVRSFRGMTLAGTLEELLNNLEYIG
CHHHHHHHHHCCCCCCCCCCEEEEEEECEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHC
SDFKYLGPVAGSTMLIRDLSVGGK
CCCHHHCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TNIKSIIEKSMKEIKSKGLKGQINVISTESKNASFSNGKLEQLTEGEKGAAGIKVISPD
CCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCCCCEEEECCC
GRTATSSSNILTEEGIMQAVEKAIEMTKYTEKDEANDISNDEQFTYIDWAFDADTKDMNL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCH
DEVMKLAQELEKKAKSEDERIKFVRGAEYEVALTRIHFGNTNGLYKNALFTNAASSISLA
HHHHHHHHHHHHHHCCCCHHEEEEECCCEEEEEEEEEECCCCCCCHHHHHHCCCCCEEEE
AIEGEDSSFGFDYQLSQSYRLIDIDRIVKNSVEMAVSGLKSEVLSSGRYDIVMSPFASSM
EEECCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
FLGTLITPLSGENVYKGKSFLKDELGEKVANSTVTLIHDPMNGSAPIIASFDNEGTNTSR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEECCCCCCCCCH
FNVIENGVLKGFLHNIYSAKKLGAKPTGNSFASVESPNPSIGTINLHFIANKTRKDILNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCEEEEEEEEEECCHHHHHHCC
PKALYVTNIMGMHTADPVSGRFSVQISGRLIENGEFVRSFRGMTLAGTLEELLNNLEYIG
CHHHHHHHHHCCCCCCCCCCEEEEEEECEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHC
SDFKYLGPVAGSTMLIRDLSVGGK
CCCHHHCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]