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The map label for this gene is ctfB [H]

Identifier: 160902537

GI number: 160902537

Start: 1150886

End: 1151536

Strand: Direct

Name: ctfB [H]

Synonym: Pmob_1076

Alternate gene names: 160902537

Gene position: 1150886-1151536 (Clockwise)

Preceding gene: 160902536

Following gene: 160902538

Centisome position: 53.05

GC content: 36.71

Gene sequence:

>651_bases
ATGAATGAAAAAGAAAGGATCGCTAAAAGGGTAGCAAAAGAATTGAAACCAGGAAATATTGTCAACTTAGGAATCGGTTT
GCCGACTTTGGTATCTAACTTTGTGTCTAAAGATGCTGAAATATTTTTCCAAGGAGAAAACGGGATTCTCGGAATAGCTT
CAGAGATTCCTGAAGAAATATCTAACCCCAACTTAATCAATGCAGGCGGAAAATATGTGGATATAATACCCGGTGCAATG
ATATTTGATTCTGCTTTTTCTTTTGCGTTAATTAGAGGAGGTCATATAGATGTAACGGTTCTTGGAGCTTTTCAAGTTGA
TCAGGAAGGACACCTAGCAAACTGGATGGTCCCCGGAAAAGTTATCGCTGGCATGGGTGGAGCAATGGACTTAGTTACCG
GTGCAAAAAAGGTAATAATAGCTATGACACACACCTCAAAAGGTACACCTAAAATAATTAAAAAGTGTTCTTTACCTTTA
ACCTCAATCAGAAGGGTTGATTTGATAGTAACCGAGTTTTCTGTCATTGAACCAACTGATAATGGGTTATTATTAAAAGA
GATTTCCCCTGACATTACTTTAGAAGAATTAAAGGAAATAACTGAAGCAGAACTAATAGTTCCAGACGACTTAAAACTTA
TGGAATTCTAA

Upstream 100 bases:

>100_bases
CCGAAGTAGATGAACTTGTTCCAACAGGTAGTATATGTCCTGATGAAATTCATATTCCTGGAGCTATTGTTGATTACGTT
GTTATAAGGAGTGAAAAAAG

Downstream 100 bases:

>100_bases
CTATAGGGAAAACCAAGCTTGAAACCTTTATATTCAATATTTCAAAGGGGGGAACAATAAATGGAAAAAGCGATTATACG
CGTAAGAATGGGTTTGCAAG

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: Coat B; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit B [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MNEKERIAKRVAKELKPGNIVNLGIGLPTLVSNFVSKDAEIFFQGENGILGIASEIPEEISNPNLINAGGKYVDIIPGAM
IFDSAFSFALIRGGHIDVTVLGAFQVDQEGHLANWMVPGKVIAGMGGAMDLVTGAKKVIIAMTHTSKGTPKIIKKCSLPL
TSIRRVDLIVTEFSVIEPTDNGLLLKEISPDITLEELKEITEAELIVPDDLKLMEF

Sequences:

>Translated_216_residues
MNEKERIAKRVAKELKPGNIVNLGIGLPTLVSNFVSKDAEIFFQGENGILGIASEIPEEISNPNLINAGGKYVDIIPGAM
IFDSAFSFALIRGGHIDVTVLGAFQVDQEGHLANWMVPGKVIAGMGGAMDLVTGAKKVIIAMTHTSKGTPKIIKKCSLPL
TSIRRVDLIVTEFSVIEPTDNGLLLKEISPDITLEELKEITEAELIVPDDLKLMEF
>Mature_216_residues
MNEKERIAKRVAKELKPGNIVNLGIGLPTLVSNFVSKDAEIFFQGENGILGIASEIPEEISNPNLINAGGKYVDIIPGAM
IFDSAFSFALIRGGHIDVTVLGAFQVDQEGHLANWMVPGKVIAGMGGAMDLVTGAKKVIIAMTHTSKGTPKIIKKCSLPL
TSIRRVDLIVTEFSVIEPTDNGLLLKEISPDITLEELKEITEAELIVPDDLKLMEF

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation [H]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=215, Percent_Identity=46.5116279069767, Blast_Score=189, Evalue=1e-48,
Organism=Homo sapiens, GI11545841, Length=217, Percent_Identity=44.7004608294931, Blast_Score=185, Evalue=3e-47,
Organism=Escherichia coli, GI1788552, Length=213, Percent_Identity=52.5821596244132, Blast_Score=230, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17531517, Length=214, Percent_Identity=44.392523364486, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=47.196261682243, Blast_Score=190, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=47.196261682243, Blast_Score=189, Evalue=1e-48,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.9 [H]

Molecular weight: Translated: 23323; Mature: 23323

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKERIAKRVAKELKPGNIVNLGIGLPTLVSNFVSKDAEIFFQGENGILGIASEIPEEI
CCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCEEEEECCCCEEECHHHHHHHH
SNPNLINAGGKYVDIIPGAMIFDSAFSFALIRGGHIDVTVLGAFQVDQEGHLANWMVPGK
CCCCEECCCCCEEEECCCHHHHHHHHHEEEEECCEEEEEEEEEEEECCCCCEEEEECCCH
VIAGMGGAMDLVTGAKKVIIAMTHTSKGTPKIIKKCSLPLTSIRRVDLIVTEFSVIEPTD
HHHCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHCCCCHHHHHEEEEEEEEEEEECCCC
NGLLLKEISPDITLEELKEITEAELIVPDDLKLMEF
CCEEEECCCCCCCHHHHHHHHHCEEECCCCCCCCCC
>Mature Secondary Structure
MNEKERIAKRVAKELKPGNIVNLGIGLPTLVSNFVSKDAEIFFQGENGILGIASEIPEEI
CCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCEEEEECCCCEEECHHHHHHHH
SNPNLINAGGKYVDIIPGAMIFDSAFSFALIRGGHIDVTVLGAFQVDQEGHLANWMVPGK
CCCCEECCCCCEEEECCCHHHHHHHHHEEEEECCEEEEEEEEEEEECCCCCEEEEECCCH
VIAGMGGAMDLVTGAKKVIIAMTHTSKGTPKIIKKCSLPLTSIRRVDLIVTEFSVIEPTD
HHHCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHCCCCHHHHHEEEEEEEEEEEECCCC
NGLLLKEISPDITLEELKEITEAELIVPDDLKLMEF
CCEEEECCCCCCCHHHHHHHHHCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254264; 8226639; 8423010; 11466286; 2383002 [H]