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The map label for this gene is rpoD [H]

Identifier: 160902123

GI number: 160902123

Start: 700150

End: 701406

Strand: Direct

Name: rpoD [H]

Synonym: Pmob_0650

Alternate gene names: 160902123

Gene position: 700150-701406 (Clockwise)

Preceding gene: 160902122

Following gene: 160902150

Centisome position: 32.27

GC content: 34.37

Gene sequence:

>1257_bases
GTGAAAAACAAAAGAATAAGTGAGAAAGAAGAGAAAAAAATTATGAAAAGCATAGAGAATATCGATTTTGAAGATGCCGA
AAAAGACGGAGATACCGTTAAAGTAAAAGTGAAAAAGTCCCACGAAGAAGCAAAATCAGTAGATACTTTCATTTCATTAT
TAATAAATAAAGCAAAGAACAAAGGCAATAAATTATCTTATTCCGATATTGATCATGCGATCCCTACAGATTTATCGGAA
GAAATTGACAGTGATTACATAGAAAAGATTTACGAGGCTTTGGAAGCAGAAGGTATAGAAATTTTAGAAGAAGAAGTTGA
AGAAGAAGAGAAAGATGCCTTAAATGAAACGGAATATGAAAATGAAGAGGTTGAAGAGTTATTTTCGGATACAGAGCTTA
AAATGTATGACAATATTTCTTTAGGTGATCCTATAAAAATATACCTCAAAGAAATAAGCAAAGTTGAGCTTTTAAGTCCT
TCCAGAGAAAGGCAACTAGCAAGACGAGCTCAAAAAGGTGATCAGAAAGCCAGAGACGAACTGATAAAGGCAAATTTAAG
GTTAGTTATCAGTATAGCCAAAAGGTACACGGGTAGAGGGTTGACTTTTTTGGATTTGATCCAAGAGGGAAATATCGGAT
TGATAAAGGCTGTAGACAAATTTGACTGGAGAAAAGGTTTCAAATTCTCAACCTATGCAACATGGTGGATTAGACAGGCA
ATTACCAGAGCCATAGCTGATCAAGCAAGAACGATTAGAATCCCTGTTCATTTAGTTGAGACTATAAACAGAATGAACAA
AGTTATCAGAGAGTATTTACAAGAAAACGGAGATTATCCTTCAGCACAAGAACTTGCAGAAATTCTAGATAAACCAGAAG
ATAAGATTAATGAAATCCTAATGAGTGCAAAAGATGTACTTTCTTTAAACTCACCTATATCTAGCGGTAATGGTGATGAT
GAAGAATCTGAAACAGGAGATTTTATTAGTACCGAAGAAACAACCCCGGAAGAAGAAGCTCAAAAAATGATTATGAAAGA
AAGACTGGAGGAAGTTCTTGACACCTTAAACTCTAAAGAAGCGTTGGTTTTGAAAATGAGATATGGATTTTTAGATGGCA
AACAAAAGACACTAGAAGAAGTTGGACAGTTCTTTAACGTCACAAGAGAAAGAATAAGGCAGATAGAAACTAAGGCGTTG
AGGAAATTGAGACATCCAAACAGAGTAGCACAACTAAAAGATATTGTAGAAAGTTAA

Upstream 100 bases:

>100_bases
CTTGTTCCGGTTCCGGGTGTGCCAAGGTGTTAAGAAGCGCTCTGTAAAGTTTTAGAGCTTTTAAAGCGAGTCTGGTTTTT
TAATGAAAGGAGGGAATTAA

Downstream 100 bases:

>100_bases
GAAAAAAGCCGCCTCGCTGAGGCGGTTTTTTAATTTTTACTTGTTTCCACCATTATACATTTGATAAAAGAATACTCTAC
GTTTGGCAATTTTTCCAAAT

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKNKRISEKEEKKIMKSIENIDFEDAEKDGDTVKVKVKKSHEEAKSVDTFISLLINKAKNKGNKLSYSDIDHAIPTDLSE
EIDSDYIEKIYEALEAEGIEILEEEVEEEEKDALNETEYENEEVEELFSDTELKMYDNISLGDPIKIYLKEISKVELLSP
SRERQLARRAQKGDQKARDELIKANLRLVISIAKRYTGRGLTFLDLIQEGNIGLIKAVDKFDWRKGFKFSTYATWWIRQA
ITRAIADQARTIRIPVHLVETINRMNKVIREYLQENGDYPSAQELAEILDKPEDKINEILMSAKDVLSLNSPISSGNGDD
EESETGDFISTEETTPEEEAQKMIMKERLEEVLDTLNSKEALVLKMRYGFLDGKQKTLEEVGQFFNVTRERIRQIETKAL
RKLRHPNRVAQLKDIVES

Sequences:

>Translated_418_residues
MKNKRISEKEEKKIMKSIENIDFEDAEKDGDTVKVKVKKSHEEAKSVDTFISLLINKAKNKGNKLSYSDIDHAIPTDLSE
EIDSDYIEKIYEALEAEGIEILEEEVEEEEKDALNETEYENEEVEELFSDTELKMYDNISLGDPIKIYLKEISKVELLSP
SRERQLARRAQKGDQKARDELIKANLRLVISIAKRYTGRGLTFLDLIQEGNIGLIKAVDKFDWRKGFKFSTYATWWIRQA
ITRAIADQARTIRIPVHLVETINRMNKVIREYLQENGDYPSAQELAEILDKPEDKINEILMSAKDVLSLNSPISSGNGDD
EESETGDFISTEETTPEEEAQKMIMKERLEEVLDTLNSKEALVLKMRYGFLDGKQKTLEEVGQFFNVTRERIRQIETKAL
RKLRHPNRVAQLKDIVES
>Mature_418_residues
MKNKRISEKEEKKIMKSIENIDFEDAEKDGDTVKVKVKKSHEEAKSVDTFISLLINKAKNKGNKLSYSDIDHAIPTDLSE
EIDSDYIEKIYEALEAEGIEILEEEVEEEEKDALNETEYENEEVEELFSDTELKMYDNISLGDPIKIYLKEISKVELLSP
SRERQLARRAQKGDQKARDELIKANLRLVISIAKRYTGRGLTFLDLIQEGNIGLIKAVDKFDWRKGFKFSTYATWWIRQA
ITRAIADQARTIRIPVHLVETINRMNKVIREYLQENGDYPSAQELAEILDKPEDKINEILMSAKDVLSLNSPISSGNGDD
EESETGDFISTEETTPEEEAQKMIMKERLEEVLDTLNSKEALVLKMRYGFLDGKQKTLEEVGQFFNVTRERIRQIETKAL
RKLRHPNRVAQLKDIVES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=275, Percent_Identity=55.6363636363636, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI1789098, Length=288, Percent_Identity=44.4444444444444, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1789871, Length=279, Percent_Identity=28.3154121863799, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788231, Length=206, Percent_Identity=28.6407766990291, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 48257; Mature: 48257

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKRISEKEEKKIMKSIENIDFEDAEKDGDTVKVKVKKSHEEAKSVDTFISLLINKAKN
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHC
KGNKLSYSDIDHAIPTDLSEEIDSDYIEKIYEALEAEGIEILEEEVEEEEKDALNETEYE
CCCCCCHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
NEEVEELFSDTELKMYDNISLGDPIKIYLKEISKVELLSPSRERQLARRAQKGDQKARDE
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHH
LIKANLRLVISIAKRYTGRGLTFLDLIQEGNIGLIKAVDKFDWRKGFKFSTYATWWIRQA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHH
ITRAIADQARTIRIPVHLVETINRMNKVIREYLQENGDYPSAQELAEILDKPEDKINEIL
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHH
MSAKDVLSLNSPISSGNGDDEESETGDFISTEETTPEEEAQKMIMKERLEEVLDTLNSKE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
ALVLKMRYGFLDGKQKTLEEVGQFFNVTRERIRQIETKALRKLRHPNRVAQLKDIVES
CEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MKNKRISEKEEKKIMKSIENIDFEDAEKDGDTVKVKVKKSHEEAKSVDTFISLLINKAKN
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHC
KGNKLSYSDIDHAIPTDLSEEIDSDYIEKIYEALEAEGIEILEEEVEEEEKDALNETEYE
CCCCCCHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
NEEVEELFSDTELKMYDNISLGDPIKIYLKEISKVELLSPSRERQLARRAQKGDQKARDE
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHH
LIKANLRLVISIAKRYTGRGLTFLDLIQEGNIGLIKAVDKFDWRKGFKFSTYATWWIRQA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHH
ITRAIADQARTIRIPVHLVETINRMNKVIREYLQENGDYPSAQELAEILDKPEDKINEIL
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHH
MSAKDVLSLNSPISSGNGDDEESETGDFISTEETTPEEEAQKMIMKERLEEVLDTLNSKE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
ALVLKMRYGFLDGKQKTLEEVGQFFNVTRERIRQIETKALRKLRHPNRVAQLKDIVES
CEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9045836; 10360571 [H]