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The map label for this gene is tgt [H]

Identifier: 160901883

GI number: 160901883

Start: 427649

End: 428776

Strand: Direct

Name: tgt [H]

Synonym: Pmob_0399

Alternate gene names: 160901883

Gene position: 427649-428776 (Clockwise)

Preceding gene: 160901882

Following gene: 160901884

Centisome position: 19.71

GC content: 34.13

Gene sequence:

>1128_bases
ATGGATGATTCACCTATAAAATTTGAATTAATTCATATTGATAGATACACTGGGGCTAGGCGTGGGCGAATCGTCACAAA
ACATGGCACTATTGAAACACCAGTTTTTATGCCTGTAGGTACGAATGCAACCGTAAAAACTTTAACTAACTCGTTATTAA
ACGATATCAATACTGAGATAATATTAGCTAATGCTTTTCATCTTTACTTAAGACCAGGCTTAGAGGTTCTTGAGCATTTT
CAAGGTCTTCATAACTTCATGAACTGGGAGAAGCCGATTTTGACAGATAGCGGAGGGTTTCAGGTATTTTCCTTACCAAA
CACTAGATTAACTGATGAAAAAGTAATTTTCAAATCTCCACTGGATGGTTCTCAAATAGAGTTAACTCCCGAGAAATCTT
TGGATATACAAAAAGTAATTGGATCAGATATTGCAATGGTTTTAGATGTATGTATAAACTCCTTGTCTGGGTACGATGCT
GTAAAAGATAGTGTGCAAAAAACTTTTAATTGGGCTTTAAGATCCAAACGATATCATAATAGCAACGGACAGGCATTATT
CGGTATAGTTCAAGGAGGCCTCTTTGAAGATTTAAGAAATCTCAGTTTGTCTCAGATAACCTCTTTAGATTTCGATGGTT
ATGCCTTAGGAGGATTAGCCGTAGGAGAAAAATACCAAGATTCTGAAAAGATTTTAAAAGCTTTTGGAAATAAATTACCT
GAAAACAAACCAAGATACATAATGGGGATAGGTTCTCCCACCATGATGTTTTTATCTGTAGAAAACGGTTTGGATATGTT
CGATTGCGTCTTACCAACAAGAATGGGAAGACACGGAACAGCATTAACCTGGAAAGGAAAACTAAACTTAAAATCCTCAG
AGGTTAAATTTGATCAAAAACCTATTGATGAAAAATGTGATTGTTATACATGTAAAAATCATTCACGTGGTTACATACAC
CATCTTTTTAAAAAAGATGAGGTACTCGGGAAAATCCTTTTAAGCATTCACAATCTGCGATTCAACATTTCTCTGGTAGA
GAAAATGAGAGAAGCTATAGAAAATGACGAATTTAAATTTTTTAAAGATGAATTTTTTAGCAGTTCAACATATTCTTTAA
CTAATTAA

Upstream 100 bases:

>100_bases
AAGAGGAAAAAGTAAGACAAAACCTTTTAAATGATACAATAGAGTTCAAGAATGAAGCGGATTCCTTGTTAAAGGATTTG
AAGGAGGAAATAAAAAATTA

Downstream 100 bases:

>100_bases
AGCACTAGAAAGGAGGGATCCCCAGTGAAATCGTTTATAAAGTACTACAATGAAATAAAACCTTTATATCAAAACAAATT
AGACCTTACCAAAAAATTCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MDDSPIKFELIHIDRYTGARRGRIVTKHGTIETPVFMPVGTNATVKTLTNSLLNDINTEIILANAFHLYLRPGLEVLEHF
QGLHNFMNWEKPILTDSGGFQVFSLPNTRLTDEKVIFKSPLDGSQIELTPEKSLDIQKVIGSDIAMVLDVCINSLSGYDA
VKDSVQKTFNWALRSKRYHNSNGQALFGIVQGGLFEDLRNLSLSQITSLDFDGYALGGLAVGEKYQDSEKILKAFGNKLP
ENKPRYIMGIGSPTMMFLSVENGLDMFDCVLPTRMGRHGTALTWKGKLNLKSSEVKFDQKPIDEKCDCYTCKNHSRGYIH
HLFKKDEVLGKILLSIHNLRFNISLVEKMREAIENDEFKFFKDEFFSSSTYSLTN

Sequences:

>Translated_375_residues
MDDSPIKFELIHIDRYTGARRGRIVTKHGTIETPVFMPVGTNATVKTLTNSLLNDINTEIILANAFHLYLRPGLEVLEHF
QGLHNFMNWEKPILTDSGGFQVFSLPNTRLTDEKVIFKSPLDGSQIELTPEKSLDIQKVIGSDIAMVLDVCINSLSGYDA
VKDSVQKTFNWALRSKRYHNSNGQALFGIVQGGLFEDLRNLSLSQITSLDFDGYALGGLAVGEKYQDSEKILKAFGNKLP
ENKPRYIMGIGSPTMMFLSVENGLDMFDCVLPTRMGRHGTALTWKGKLNLKSSEVKFDQKPIDEKCDCYTCKNHSRGYIH
HLFKKDEVLGKILLSIHNLRFNISLVEKMREAIENDEFKFFKDEFFSSSTYSLTN
>Mature_375_residues
MDDSPIKFELIHIDRYTGARRGRIVTKHGTIETPVFMPVGTNATVKTLTNSLLNDINTEIILANAFHLYLRPGLEVLEHF
QGLHNFMNWEKPILTDSGGFQVFSLPNTRLTDEKVIFKSPLDGSQIELTPEKSLDIQKVIGSDIAMVLDVCINSLSGYDA
VKDSVQKTFNWALRSKRYHNSNGQALFGIVQGGLFEDLRNLSLSQITSLDFDGYALGGLAVGEKYQDSEKILKAFGNKLP
ENKPRYIMGIGSPTMMFLSVENGLDMFDCVLPTRMGRHGTALTWKGKLNLKSSEVKFDQKPIDEKCDCYTCKNHSRGYIH
HLFKKDEVLGKILLSIHNLRFNISLVEKMREAIENDEFKFFKDEFFSSSTYSLTN

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=370, Percent_Identity=40, Blast_Score=294, Evalue=8e-80,
Organism=Homo sapiens, GI13375872, Length=400, Percent_Identity=23.75, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1786607, Length=365, Percent_Identity=46.3013698630137, Blast_Score=349, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17544680, Length=350, Percent_Identity=41.7142857142857, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI25146502, Length=230, Percent_Identity=28.2608695652174, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=365, Percent_Identity=42.4657534246575, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI21355311, Length=250, Percent_Identity=26, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42409; Mature: 42409

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDSPIKFELIHIDRYTGARRGRIVTKHGTIETPVFMPVGTNATVKTLTNSLLNDINTEI
CCCCCEEEEEEEEECCCCCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEE
ILANAFHLYLRPGLEVLEHFQGLHNFMNWEKPILTDSGGFQVFSLPNTRLTDEKVIFKSP
EEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCCCCEEEEECC
LDGSQIELTPEKSLDIQKVIGSDIAMVLDVCINSLSGYDAVKDSVQKTFNWALRSKRYHN
CCCCEEEECCCCCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEC
SNGQALFGIVQGGLFEDLRNLSLSQITSLDFDGYALGGLAVGEKYQDSEKILKAFGNKLP
CCCCEEEEEHHCHHHHHHHCCCHHHHHCCCCCCEEECCEEECCCCCCHHHHHHHHHHCCC
ENKPRYIMGIGSPTMMFLSVENGLDMFDCVLPTRMGRHGTALTWKGKLNLKSSEVKFDQK
CCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECEECCCCCCCCCCCC
PIDEKCDCYTCKNHSRGYIHHLFKKDEVLGKILLSIHNLRFNISLVEKMREAIENDEFKF
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCEE
FKDEFFSSSTYSLTN
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MDDSPIKFELIHIDRYTGARRGRIVTKHGTIETPVFMPVGTNATVKTLTNSLLNDINTEI
CCCCCEEEEEEEEECCCCCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEE
ILANAFHLYLRPGLEVLEHFQGLHNFMNWEKPILTDSGGFQVFSLPNTRLTDEKVIFKSP
EEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCCCCEEEEECC
LDGSQIELTPEKSLDIQKVIGSDIAMVLDVCINSLSGYDAVKDSVQKTFNWALRSKRYHN
CCCCEEEECCCCCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEC
SNGQALFGIVQGGLFEDLRNLSLSQITSLDFDGYALGGLAVGEKYQDSEKILKAFGNKLP
CCCCEEEEEHHCHHHHHHHCCCHHHHHCCCCCCEEECCEEECCCCCCHHHHHHHHHHCCC
ENKPRYIMGIGSPTMMFLSVENGLDMFDCVLPTRMGRHGTALTWKGKLNLKSSEVKFDQK
CCCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECEECCCCCCCCCCCC
PIDEKCDCYTCKNHSRGYIHHLFKKDEVLGKILLSIHNLRFNISLVEKMREAIENDEFKF
CCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHCCCCCEE
FKDEFFSSSTYSLTN
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]