Click here to switch to the map view.

The map label for this gene is tvaI [H]

Identifier: 160901853

GI number: 160901853

Start: 397388

End: 399367

Strand: Direct

Name: tvaI [H]

Synonym: Pmob_0369

Alternate gene names: 160901853

Gene position: 397388-399367 (Clockwise)

Preceding gene: 160901852

Following gene: 160901872

Centisome position: 18.32

GC content: 33.79

Gene sequence:

>1980_bases
ATGAAAGGCATATATTCTGATCAAACTAGCTGTTATCTTTATCCCGAGGAGCCAAGCATAGATGACAAGGTAACGATTAA
ACTGAGAATTCCAAAATACTTAGGTAAAAGTATCGGAAGTGTTATTTTTACTCCTCAAAAAAACTTAAAAAATTATCAAC
ATAAACCAATGCAACTCTCAAAAGAAACTACTTATTTTTATTTTTTTGAAAGTACTTTCAAAATGCCAGACAGAATTGTT
AGGTACCATTTCGAAATAGATTTAATAGAAAAGGGCAAAAAAATGTTCTATGACGCTATGGGGATTGTAGAAAATCGCAC
TATTCATGACTTTGTACTCGTTGCAGATTTTAAAACTCCAAAATGGTCCCATGGTTCAATATATTATCAAATATTTGTTG
ATCGATTTAAAAACGGAGATGAAACAAACGATCCAGTATCTCACGAGTATCAATACGATGGTCAAGAAGTTTTGAAAAAA
GATTGGAACTCACTGCCAGATCCTAAAAATGGTCATAGAGAGTTTTATGGCGGAGATTTACAAGGAGTTTTAGAAAAGAT
TGACTACTTAAAAGATTTAGGAGTGGAAACGATTTACCTAAACCCTATCTTTGTTTCTCCAAGTCCGCACAAATATGATA
CTCAAGATTACGAACATGTAGACCCTCATTTTGGGGTTATAGAGGAAGATTCTGAAGATCTTAACGAAAAATACAAAGTC
AGAACTACCTCTATAAAGAACCTCGAAAAAAGCGATGAAATTCTAAAAAGTCTTATTCAAAAAGCCCATGAAAAAGGTAT
AAAAGTTATTTTAGATGGCGTTTTCAACCATTGTGGCTCTTTTCATAAGTGGGTAGACGAAATGGATTTGTATGGTGAAG
GTAGTCTACATAGGGATGATTCTCCCTACAAAAGTTATTTTTATTGGGACGGTACCCAAAAGAGCTATGAGGGATGGTGG
GGTTTTCACACCCTACCAAAATTGAATTATGGAAATATTAGTTTATGGAAATACATAGCTGATATTGGGAAAAAATGGGT
AAGTGAACCTTTCAATGCGGATGGATGGAGACTCGATGTTGCGGATGATTTAGGTAAATCTTTTGAAATGAACACAGCTT
TTTGGAGATTCTTCTACAAAGTTGTAAAAAAGTCAAATCCAGAATCGATAATATTTGCAGAAATTTATAAATCACCTCTT
GCTTGGTTAGAAAGACAATGCTGGGACTCTATAATGAATTATATAACCTGTATGGATCCGGTTAGTTATTTTCTTACAGG
CATGGAAAAACACAACGAGCATTATAAACCTGAGCTCTTAAAAAATGCTGAGTATTTCGTTAACTCAGTGAGATGGGCTT
TATCTCAATTACCCATGAATAGTAAATTTATTGCTTTAAACCAACTAAGCAACCATGATCATTCTAGATGGATGACAAGA
ACCACACAAAAAGTTGGGAGATTGGGACCAAAAACTCATGAAGAAGCTTCCCTTGGAAAGGATTTAGATGTTTTCAAAAT
AGGGTTAGTCACAATGTTTACTCTTCCAGGATCACCCGGATTATTCTACGGTGATGAAATAGGTTTAGAAGGTTGGACAG
ACCCAGATAATAGAAGACCTTACCCTTGGGGAAAAGAAAGTGAAGAAAATAAAATGTTGTTTAATTTCACAAAAGAATTA
ATTAAAATGTACAAAGAACATCTTGCATTGAGAAAGGGGTCTTTTGATTTTCTTGATTGGAATGGAGGATACGTATCTTA
CGCTTCATGGAATGAAAGTGAAAATATAATCACCGTTATAAATAGAGAAGAAAAAGAGATCGATGTTGAACTGCCATTAT
GGCTACTCGATAAAAAAGAAGGGAAAATAGATCTTTTATTCGCTACAAAAGATTTCAATTTAGAAGATAATTCTTATAAC
GACGGCAAAAAATCTCTAAAAATCCCAGAGAAAATTGCCCTTGTATTTAAAATTAATTGA

Upstream 100 bases:

>100_bases
TAGAAAAGGAACTTACAAAACTACAAAAACTGGAAAGTAAATATGTAGAAGAATTAAATCAACATACTAAAATCTCAAAC
AATAATTACGGAGGATAATT

Downstream 100 bases:

>100_bases
TTCCCCTTGCTTGGATATATGGCTTTTTTTAATGTTCTGTAATCTCACCCTTTAAAATATTAATTCCGTGTGGGATAAGA
TCTTTAACAGCTTCAAAACA

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: Alpha-amylase I; TVA I [H]

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MKGIYSDQTSCYLYPEEPSIDDKVTIKLRIPKYLGKSIGSVIFTPQKNLKNYQHKPMQLSKETTYFYFFESTFKMPDRIV
RYHFEIDLIEKGKKMFYDAMGIVENRTIHDFVLVADFKTPKWSHGSIYYQIFVDRFKNGDETNDPVSHEYQYDGQEVLKK
DWNSLPDPKNGHREFYGGDLQGVLEKIDYLKDLGVETIYLNPIFVSPSPHKYDTQDYEHVDPHFGVIEEDSEDLNEKYKV
RTTSIKNLEKSDEILKSLIQKAHEKGIKVILDGVFNHCGSFHKWVDEMDLYGEGSLHRDDSPYKSYFYWDGTQKSYEGWW
GFHTLPKLNYGNISLWKYIADIGKKWVSEPFNADGWRLDVADDLGKSFEMNTAFWRFFYKVVKKSNPESIIFAEIYKSPL
AWLERQCWDSIMNYITCMDPVSYFLTGMEKHNEHYKPELLKNAEYFVNSVRWALSQLPMNSKFIALNQLSNHDHSRWMTR
TTQKVGRLGPKTHEEASLGKDLDVFKIGLVTMFTLPGSPGLFYGDEIGLEGWTDPDNRRPYPWGKESEENKMLFNFTKEL
IKMYKEHLALRKGSFDFLDWNGGYVSYASWNESENIITVINREEKEIDVELPLWLLDKKEGKIDLLFATKDFNLEDNSYN
DGKKSLKIPEKIALVFKIN

Sequences:

>Translated_659_residues
MKGIYSDQTSCYLYPEEPSIDDKVTIKLRIPKYLGKSIGSVIFTPQKNLKNYQHKPMQLSKETTYFYFFESTFKMPDRIV
RYHFEIDLIEKGKKMFYDAMGIVENRTIHDFVLVADFKTPKWSHGSIYYQIFVDRFKNGDETNDPVSHEYQYDGQEVLKK
DWNSLPDPKNGHREFYGGDLQGVLEKIDYLKDLGVETIYLNPIFVSPSPHKYDTQDYEHVDPHFGVIEEDSEDLNEKYKV
RTTSIKNLEKSDEILKSLIQKAHEKGIKVILDGVFNHCGSFHKWVDEMDLYGEGSLHRDDSPYKSYFYWDGTQKSYEGWW
GFHTLPKLNYGNISLWKYIADIGKKWVSEPFNADGWRLDVADDLGKSFEMNTAFWRFFYKVVKKSNPESIIFAEIYKSPL
AWLERQCWDSIMNYITCMDPVSYFLTGMEKHNEHYKPELLKNAEYFVNSVRWALSQLPMNSKFIALNQLSNHDHSRWMTR
TTQKVGRLGPKTHEEASLGKDLDVFKIGLVTMFTLPGSPGLFYGDEIGLEGWTDPDNRRPYPWGKESEENKMLFNFTKEL
IKMYKEHLALRKGSFDFLDWNGGYVSYASWNESENIITVINREEKEIDVELPLWLLDKKEGKIDLLFATKDFNLEDNSYN
DGKKSLKIPEKIALVFKIN
>Mature_659_residues
MKGIYSDQTSCYLYPEEPSIDDKVTIKLRIPKYLGKSIGSVIFTPQKNLKNYQHKPMQLSKETTYFYFFESTFKMPDRIV
RYHFEIDLIEKGKKMFYDAMGIVENRTIHDFVLVADFKTPKWSHGSIYYQIFVDRFKNGDETNDPVSHEYQYDGQEVLKK
DWNSLPDPKNGHREFYGGDLQGVLEKIDYLKDLGVETIYLNPIFVSPSPHKYDTQDYEHVDPHFGVIEEDSEDLNEKYKV
RTTSIKNLEKSDEILKSLIQKAHEKGIKVILDGVFNHCGSFHKWVDEMDLYGEGSLHRDDSPYKSYFYWDGTQKSYEGWW
GFHTLPKLNYGNISLWKYIADIGKKWVSEPFNADGWRLDVADDLGKSFEMNTAFWRFFYKVVKKSNPESIIFAEIYKSPL
AWLERQCWDSIMNYITCMDPVSYFLTGMEKHNEHYKPELLKNAEYFVNSVRWALSQLPMNSKFIALNQLSNHDHSRWMTR
TTQKVGRLGPKTHEEASLGKDLDVFKIGLVTMFTLPGSPGLFYGDEIGLEGWTDPDNRRPYPWGKESEENKMLFNFTKEL
IKMYKEHLALRKGSFDFLDWNGGYVSYASWNESENIITVINREEKEIDVELPLWLLDKKEGKIDLLFATKDFNLEDNSYN
DGKKSLKIPEKIALVFKIN

Specific function: Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Also hydrolyzes cyclodextrins [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=615, Percent_Identity=29.2682926829268, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI1790687, Length=133, Percent_Identity=32.3308270676692, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789995, Length=236, Percent_Identity=24.5762711864407, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322245, Length=133, Percent_Identity=32.3308270676692, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319776, Length=133, Percent_Identity=32.3308270676692, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321731, Length=133, Percent_Identity=32.3308270676692, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321726, Length=191, Percent_Identity=26.7015706806283, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324416, Length=133, Percent_Identity=30.0751879699248, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322241, Length=133, Percent_Identity=30.0751879699248, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=133, Percent_Identity=30.0751879699248, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24586591, Length=212, Percent_Identity=26.4150943396226, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586589, Length=393, Percent_Identity=22.64631043257, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586587, Length=402, Percent_Identity=23.134328358209, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583745, Length=204, Percent_Identity=25.4901960784314, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 77336; Mature: 77336

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIYSDQTSCYLYPEEPSIDDKVTIKLRIPKYLGKSIGSVIFTPQKNLKNYQHKPMQLS
CCCCCCCCCEEEEECCCCCCCCEEEEEEECCHHHHHHHCCEEECCCHHHHHCCCCCCEEC
KETTYFYFFESTFKMPDRIVRYHFEIDLIEKGKKMFYDAMGIVENRTIHDFVLVADFKTP
CCCEEEEEEECCCCCCHHHEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
KWSHGSIYYQIFVDRFKNGDETNDPVSHEYQYDGQEVLKKDWNSLPDPKNGHREFYGGDL
CCCCCCEEEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCH
QGVLEKIDYLKDLGVETIYLNPIFVSPSPHKYDTQDYEHVDPHFGVIEEDSEDLNEKYKV
HHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCHHHCCCEEE
RTTSIKNLEKSDEILKSLIQKAHEKGIKVILDGVFNHCGSFHKWVDEMDLYGEGSLHRDD
EEHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCC
SPYKSYFYWDGTQKSYEGWWGFHTLPKLNYGNISLWKYIADIGKKWVSEPFNADGWRLDV
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEC
ADDLGKSFEMNTAFWRFFYKVVKKSNPESIIFAEIYKSPLAWLERQCWDSIMNYITCMDP
HHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSYFLTGMEKHNEHYKPELLKNAEYFVNSVRWALSQLPMNSKFIALNQLSNHDHSRWMTR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
TTQKVGRLGPKTHEEASLGKDLDVFKIGLVTMFTLPGSPGLFYGDEIGLEGWTDPDNRRP
HHHHHHCCCCCCCCHHHCCCCCHHHEEEEEEEEECCCCCCCEECCCCCCCCCCCCCCCCC
YPWGKESEENKMLFNFTKELIKMYKEHLALRKGSFDFLDWNGGYVSYASWNESENIITVI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCCEEEEE
NREEKEIDVELPLWLLDKKEGKIDLLFATKDFNLEDNSYNDGKKSLKIPEKIALVFKIN
ECCCCEEEEECCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MKGIYSDQTSCYLYPEEPSIDDKVTIKLRIPKYLGKSIGSVIFTPQKNLKNYQHKPMQLS
CCCCCCCCCEEEEECCCCCCCCEEEEEEECCHHHHHHHCCEEECCCHHHHHCCCCCCEEC
KETTYFYFFESTFKMPDRIVRYHFEIDLIEKGKKMFYDAMGIVENRTIHDFVLVADFKTP
CCCEEEEEEECCCCCCHHHEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
KWSHGSIYYQIFVDRFKNGDETNDPVSHEYQYDGQEVLKKDWNSLPDPKNGHREFYGGDL
CCCCCCEEEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCH
QGVLEKIDYLKDLGVETIYLNPIFVSPSPHKYDTQDYEHVDPHFGVIEEDSEDLNEKYKV
HHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCHHHCCCEEE
RTTSIKNLEKSDEILKSLIQKAHEKGIKVILDGVFNHCGSFHKWVDEMDLYGEGSLHRDD
EEHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCC
SPYKSYFYWDGTQKSYEGWWGFHTLPKLNYGNISLWKYIADIGKKWVSEPFNADGWRLDV
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEC
ADDLGKSFEMNTAFWRFFYKVVKKSNPESIIFAEIYKSPLAWLERQCWDSIMNYITCMDP
HHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSYFLTGMEKHNEHYKPELLKNAEYFVNSVRWALSQLPMNSKFIALNQLSNHDHSRWMTR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
TTQKVGRLGPKTHEEASLGKDLDVFKIGLVTMFTLPGSPGLFYGDEIGLEGWTDPDNRRP
HHHHHHCCCCCCCCHHHCCCCCHHHEEEEEEEEECCCCCCCEECCCCCCCCCCCCCCCCC
YPWGKESEENKMLFNFTKELIKMYKEHLALRKGSFDFLDWNGGYVSYASWNESENIITVI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCCCCCEEEEE
NREEKEIDVELPLWLLDKKEGKIDLLFATKDFNLEDNSYNDGKKSLKIPEKIALVFKIN
ECCCCEEEEECCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7548164; 12051850 [H]