Click here to switch to the map view.

The map label for this gene is aglA [H]

Identifier: 160901617

GI number: 160901617

Start: 127349

End: 128803

Strand: Direct

Name: aglA [H]

Synonym: Pmob_0127

Alternate gene names: 160901617

Gene position: 127349-128803 (Clockwise)

Preceding gene: 160901616

Following gene: 160901618

Centisome position: 5.87

GC content: 37.11

Gene sequence:

>1455_bases
ATGACAGCTATCAAGGTAGGGATCATTGGAGCAGGAAGCGCAGCATTTTCTTTAAGATTGGTTAGCGATCTTTGTAAAAC
AAAGGGATTATCAGGGAGTCTAGTATCCTTAATGGATATAGATAAAGACAGATTGAACGCAGTACACATGCTTGCTATGA
AATTTGCAGAAGAGTTCGGAGCGGATTTGAGGTTTGAAACTACAACAAACGTTGAAGACGCAATAAAAGACTCAAGTTTT
GTTGTAAATACTGCCCTTGTTGGAGGGCACAGTTACTTTGAACAAGTAAGAAAAATTTCAGAAAAATACGGATATTACAG
AGGAATAGACTCACAAGAATTCAACATGGTTTCAGATTATTACACAATAAGCAATTTCAACCAATTAAAGTTTATGCACG
ATGTTGCAAAAGCTATTGAAAGAATATCCCCTAAGGCATGGCTTTTGCAGGCGGCAAATCCTGTCTTTGAATTGACAAAT
TTAATAACAAGAACGGTTCCAATAAACATGGTTGGAATATGCCACGGCCATCATGGAGTAGACCACATAATTGAAAAATT
AGGCTTAGATGCAGAAAAAGTTGAATGGCAAGTAGCAGGTGTAAACCATGGAATTTGGTTGACAAAATTCATGTATGAAG
GAAAAGATGCCTATCCACTCATTGATGAATTATTAGAAAAAGAGGTTGAAAACTTCAAACCCACCAATCCATTCGACGAT
CAACTCTCTCCAGTTGCAAAAGATATGTATGAATTCTATGGAAAAATGCCCATTGGAGACACAGTCAGGAACGGAAGTTG
GAAATATCATTACAACCTCGAAACGAAGAAAAAGTGGTTTGGAGAACCATGGGGAGGCGTTGATTCAGAGTTAGGATGGA
AATGGTATCAAGACAGACAAGCAGAGATTGCAATAGCAATGCAAAAAGTTGCCAAATATTTCCAAGAGAATAAGAACGCC
AAATTACTTTCAAAAGATTCACTCAATGAAATTATCTCTCAAACCAAAAACGATGTAAAAGAAGAACTCACAAAAGAAAT
ATACAACTTGTTAGATCCTCAAAGGAAAAGCGGCGAACAACATATATTGTTGGCAAATGCCTTATTAAACAACGAAAAAG
TAGATCTCGTTCTCAATTTACCAAATAACGGTACTATACCTGGAATCCCAGACGATGTAGCCGTGGAGATTCCTGTGTAT
GCAGACAAAGATGGAATCCATCGTTACAAAATAGATCCACCACTTCCAGAGAGAATTAAAAAGATGTACTTATACCCAAG
AATTATGAGAATGGAATGGGCATTAGAAGCATTCCTAACTGGAGATAAAAAAGTACTCGAAGAATTTTTGATCAGAGATC
CACGTACCAAATCTTACGACCAGGTAGTAAAAGTTTTAGAGGAAATCCTTGCTTTGCCAGGTAATGAAGAAATGAGGAAG
CACTACAGCAAATAA

Upstream 100 bases:

>100_bases
ATTAAAAAAATAGATAACAATATCATAACGATTTACACTATAAACAACATTATAGCAACAAAAAAGTTCAATAGTTGATA
AAAGAGGAGGAAAGATGTAA

Downstream 100 bases:

>100_bases
TAGTTTAATTAAGGGGGCTAATGCCCCCTTAATTTAATAAACAAATCTATCTAAATTTTGTATAAATCTTCGTTATAAGT
GTATTTTTACGGTCGATTAT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Maltase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTAIKVGIIGAGSAAFSLRLVSDLCKTKGLSGSLVSLMDIDKDRLNAVHMLAMKFAEEFGADLRFETTTNVEDAIKDSSF
VVNTALVGGHSYFEQVRKISEKYGYYRGIDSQEFNMVSDYYTISNFNQLKFMHDVAKAIERISPKAWLLQAANPVFELTN
LITRTVPINMVGICHGHHGVDHIIEKLGLDAEKVEWQVAGVNHGIWLTKFMYEGKDAYPLIDELLEKEVENFKPTNPFDD
QLSPVAKDMYEFYGKMPIGDTVRNGSWKYHYNLETKKKWFGEPWGGVDSELGWKWYQDRQAEIAIAMQKVAKYFQENKNA
KLLSKDSLNEIISQTKNDVKEELTKEIYNLLDPQRKSGEQHILLANALLNNEKVDLVLNLPNNGTIPGIPDDVAVEIPVY
ADKDGIHRYKIDPPLPERIKKMYLYPRIMRMEWALEAFLTGDKKVLEEFLIRDPRTKSYDQVVKVLEEILALPGNEEMRK
HYSK

Sequences:

>Translated_484_residues
MTAIKVGIIGAGSAAFSLRLVSDLCKTKGLSGSLVSLMDIDKDRLNAVHMLAMKFAEEFGADLRFETTTNVEDAIKDSSF
VVNTALVGGHSYFEQVRKISEKYGYYRGIDSQEFNMVSDYYTISNFNQLKFMHDVAKAIERISPKAWLLQAANPVFELTN
LITRTVPINMVGICHGHHGVDHIIEKLGLDAEKVEWQVAGVNHGIWLTKFMYEGKDAYPLIDELLEKEVENFKPTNPFDD
QLSPVAKDMYEFYGKMPIGDTVRNGSWKYHYNLETKKKWFGEPWGGVDSELGWKWYQDRQAEIAIAMQKVAKYFQENKNA
KLLSKDSLNEIISQTKNDVKEELTKEIYNLLDPQRKSGEQHILLANALLNNEKVDLVLNLPNNGTIPGIPDDVAVEIPVY
ADKDGIHRYKIDPPLPERIKKMYLYPRIMRMEWALEAFLTGDKKVLEEFLIRDPRTKSYDQVVKVLEEILALPGNEEMRK
HYSK
>Mature_483_residues
TAIKVGIIGAGSAAFSLRLVSDLCKTKGLSGSLVSLMDIDKDRLNAVHMLAMKFAEEFGADLRFETTTNVEDAIKDSSFV
VNTALVGGHSYFEQVRKISEKYGYYRGIDSQEFNMVSDYYTISNFNQLKFMHDVAKAIERISPKAWLLQAANPVFELTNL
ITRTVPINMVGICHGHHGVDHIIEKLGLDAEKVEWQVAGVNHGIWLTKFMYEGKDAYPLIDELLEKEVENFKPTNPFDDQ
LSPVAKDMYEFYGKMPIGDTVRNGSWKYHYNLETKKKWFGEPWGGVDSELGWKWYQDRQAEIAIAMQKVAKYFQENKNAK
LLSKDSLNEIISQTKNDVKEELTKEIYNLLDPQRKSGEQHILLANALLNNEKVDLVLNLPNNGTIPGIPDDVAVEIPVYA
DKDGIHRYKIDPPLPERIKKMYLYPRIMRMEWALEAFLTGDKKVLEEFLIRDPRTKSYDQVVKVLEEILALPGNEEMRKH
YSK

Specific function: Alpha-glycosidase with a very broad specificity. Hydrolyzes maltose and other small maltooligosaccharides but is inactive against the polymeric substrate starch. AglA is not specific with respect to the configuration at the C-4 position of its substrates

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=487, Percent_Identity=22.3819301848049, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788029, Length=410, Percent_Identity=22.6829268292683, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 55356; Mature: 55225

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIKVGIIGAGSAAFSLRLVSDLCKTKGLSGSLVSLMDIDKDRLNAVHMLAMKFAEEFG
CCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHC
ADLRFETTTNVEDAIKDSSFVVNTALVGGHSYFEQVRKISEKYGYYRGIDSQEFNMVSDY
CCEEEECCCCHHHHHCCCCEEEEHHEECCHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHH
YTISNFNQLKFMHDVAKAIERISPKAWLLQAANPVFELTNLITRTVPINMVGICHGHHGV
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCH
DHIIEKLGLDAEKVEWQVAGVNHGIWLTKFMYEGKDAYPLIDELLEKEVENFKPTNPFDD
HHHHHHHCCCHHHCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCH
QLSPVAKDMYEFYGKMPIGDTVRNGSWKYHYNLETKKKWFGEPWGGVDSELGWKWYQDRQ
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHCCH
AEIAIAMQKVAKYFQENKNAKLLSKDSLNEIISQTKNDVKEELTKEIYNLLDPQRKSGEQ
HHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCC
HILLANALLNNEKVDLVLNLPNNGTIPGIPDDVAVEIPVYADKDGIHRYKIDPPLPERIK
EEEEEHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCHHHHH
KMYLYPRIMRMEWALEAFLTGDKKVLEEFLIRDPRTKSYDQVVKVLEEILALPGNEEMRK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
HYSK
HCCC
>Mature Secondary Structure 
TAIKVGIIGAGSAAFSLRLVSDLCKTKGLSGSLVSLMDIDKDRLNAVHMLAMKFAEEFG
CEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHC
ADLRFETTTNVEDAIKDSSFVVNTALVGGHSYFEQVRKISEKYGYYRGIDSQEFNMVSDY
CCEEEECCCCHHHHHCCCCEEEEHHEECCHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHH
YTISNFNQLKFMHDVAKAIERISPKAWLLQAANPVFELTNLITRTVPINMVGICHGHHGV
HHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCH
DHIIEKLGLDAEKVEWQVAGVNHGIWLTKFMYEGKDAYPLIDELLEKEVENFKPTNPFDD
HHHHHHHCCCHHHCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCH
QLSPVAKDMYEFYGKMPIGDTVRNGSWKYHYNLETKKKWFGEPWGGVDSELGWKWYQDRQ
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHCCH
AEIAIAMQKVAKYFQENKNAKLLSKDSLNEIISQTKNDVKEELTKEIYNLLDPQRKSGEQ
HHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCC
HILLANALLNNEKVDLVLNLPNNGTIPGIPDDVAVEIPVYADKDGIHRYKIDPPLPERIK
EEEEEHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCHHHHH
KMYLYPRIMRMEWALEAFLTGDKKVLEEFLIRDPRTKSYDQVVKVLEEILALPGNEEMRK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
HYSK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9453151; 10360571; 10972187; 12062450; 12588867 [H]