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The map label for this gene is mexA [H]

Identifier: 160877487

GI number: 160877487

Start: 5183021

End: 5184166

Strand: Reverse

Name: mexA [H]

Synonym: Sbal195_4385

Alternate gene names: 160877487

Gene position: 5184166-5183021 (Counterclockwise)

Preceding gene: 160877491

Following gene: 160877486

Centisome position: 96.95

GC content: 47.64

Gene sequence:

>1146_bases
ATGCGGCAGACAATTAAACTTGCCTCAGTTATAAGCGTGGCGTTATGGATGGCAGCTTGCAGCCCTCAAGAAGAGAAAGC
TCAGGCGGGCGCTGGCCCACGTAGTATGGAAGTGGGAATCCTTAATGTTGTTGCCCAGCCACAAGTGATTCAGGTGGAGT
TACCAGGACGCAGTAAAGCTTTCCTCGAGGCCGAAGTTCGTCCACAGGTGAGCGGTATTATCACTAAGCGTAGTTTTGTT
GAAGGTGGTAATGTGAAACAGGGTGAATCCTTGTATCAAATTGACTCTGCAACTTATAAAGCCGCGTTAGTGAGTGCCAA
TGCCGATTTAGCCAGAGCCAATGCGGGTTTAGCCTCAGCAAAAGCTAAGGCTGCACGTTATCAGCAACTGGTGAAAACCA
ACGCCATCAGTAAGCAAGAATTTGATGAAGCTGAAGCGGCATATAAAGAAGCATTAGCGAATGTCACCGTGGCAGAAGCT
GCGATTAACACCGCTAAAATCAACCTGCAATACACTGAAGTATTAGCGCCTATCTCTGGTCGTATCGGTAAATCGAGTGT
GACTGCGGGTGCGTTAGTGACAGCGAACCAAAGCCAAACGCTGGCGACGATTCAACAACTTGATCCAATCAATGTGGATA
TCGCGCAATCGAGTGCGCAGTTGTTACGTCTTAAAGCTAAGTTGAAGCAAGGTAAGCTACAAGCTTCAGAAAATGCTGAT
GTGCAGCTGTTGCTCGAAGATGGCACACAATACAGCCATCCAGGTAAGTTGCAGTTCGCCGAAGTGAGTGTGGACCCAAA
TACTGGTTCAGTAATTCTGCGCGCCGAGTTCCCGAACCCTGAAGGTATCTTGTTACCTGGTATGTATGTTCGCGCTATGT
TGAATGCGGGCACCGATCCACAGGCTATCTTGGTTCCACAAAAAGCCATCACGCGTAACTCTAAGGGTCAAGCAGTGGCT
ATGGTTGTAAATGACCAAGGTTTAGTCGAATTGAAAATTGTGACGACCGCTGAAGTGATTGACAACCAATGGCGAGTCAC
TTCTGGTTTAGCTGTGGGCGATAAACTGATTGTGGAAGGATTGCAGAAAATTCGTCCAGGCGCTCCAGTAACCACCAAAG
TTATTTCTGAAACGCAAGCACAATAG

Upstream 100 bases:

>100_bases
TATGAGATAAATAACGGATTTGATTTTAGTTACAATTACGGCTCGTGTCAGCGCAATAAGTGTACTGGTATGTGGGCAGT
TAATATTTGAAGGAGTTCTC

Downstream 100 bases:

>100_bases
GGCATAGCTATGGCACGTTTTTTTATTGATCGCCCCATCTTTGCGTGGGTGATCGCCTTGATTATTATGCTGGCGGGGAT
CCTCTCGATCCGCTCGCTGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRQTIKLASVISVALWMAACSPQEEKAQAGAGPRSMEVGILNVVAQPQVIQVELPGRSKAFLEAEVRPQVSGIITKRSFV
EGGNVKQGESLYQIDSATYKAALVSANADLARANAGLASAKAKAARYQQLVKTNAISKQEFDEAEAAYKEALANVTVAEA
AINTAKINLQYTEVLAPISGRIGKSSVTAGALVTANQSQTLATIQQLDPINVDIAQSSAQLLRLKAKLKQGKLQASENAD
VQLLLEDGTQYSHPGKLQFAEVSVDPNTGSVILRAEFPNPEGILLPGMYVRAMLNAGTDPQAILVPQKAITRNSKGQAVA
MVVNDQGLVELKIVTTAEVIDNQWRVTSGLAVGDKLIVEGLQKIRPGAPVTTKVISETQAQ

Sequences:

>Translated_381_residues
MRQTIKLASVISVALWMAACSPQEEKAQAGAGPRSMEVGILNVVAQPQVIQVELPGRSKAFLEAEVRPQVSGIITKRSFV
EGGNVKQGESLYQIDSATYKAALVSANADLARANAGLASAKAKAARYQQLVKTNAISKQEFDEAEAAYKEALANVTVAEA
AINTAKINLQYTEVLAPISGRIGKSSVTAGALVTANQSQTLATIQQLDPINVDIAQSSAQLLRLKAKLKQGKLQASENAD
VQLLLEDGTQYSHPGKLQFAEVSVDPNTGSVILRAEFPNPEGILLPGMYVRAMLNAGTDPQAILVPQKAITRNSKGQAVA
MVVNDQGLVELKIVTTAEVIDNQWRVTSGLAVGDKLIVEGLQKIRPGAPVTTKVISETQAQ
>Mature_381_residues
MRQTIKLASVISVALWMAACSPQEEKAQAGAGPRSMEVGILNVVAQPQVIQVELPGRSKAFLEAEVRPQVSGIITKRSFV
EGGNVKQGESLYQIDSATYKAALVSANADLARANAGLASAKAKAARYQQLVKTNAISKQEFDEAEAAYKEALANVTVAEA
AINTAKINLQYTEVLAPISGRIGKSSVTAGALVTANQSQTLATIQQLDPINVDIAQSSAQLLRLKAKLKQGKLQASENAD
VQLLLEDGTQYSHPGKLQFAEVSVDPNTGSVILRAEFPNPEGILLPGMYVRAMLNAGTDPQAILVPQKAITRNSKGQAVA
MVVNDQGLVELKIVTTAEVIDNQWRVTSGLAVGDKLIVEGLQKIRPGAPVTTKVISETQAQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=364, Percent_Identity=49.4505494505495, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI1789929, Length=374, Percent_Identity=45.7219251336898, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI1786668, Length=362, Percent_Identity=50, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI87082036, Length=360, Percent_Identity=28.0555555555556, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081796, Length=332, Percent_Identity=25.3012048192771, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789637, Length=149, Percent_Identity=31.5436241610738, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081951, Length=149, Percent_Identity=32.8859060402685, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40385; Mature: 40385

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQTIKLASVISVALWMAACSPQEEKAQAGAGPRSMEVGILNVVAQPQVIQVELPGRSKA
CCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEEECCCEEEEEECCCCCCE
FLEAEVRPQVSGIITKRSFVEGGNVKQGESLYQIDSATYKAALVSANADLARANAGLASA
EEEEECCCCHHHEEEHHHCCCCCCCCCCCCEEEECCCCEEEEEEECCCCHHHCCCCHHHH
KAKAARYQQLVKTNAISKQEFDEAEAAYKEALANVTVAEAAINTAKINLQYTEVLAPISG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHCEEEEEEEEHHHHHHCCCC
RIGKSSVTAGALVTANQSQTLATIQQLDPINVDIAQSSAQLLRLKAKLKQGKLQASENAD
CCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCC
VQLLLEDGTQYSHPGKLQFAEVSVDPNTGSVILRAEFPNPEGILLPGMYVRAMLNAGTDP
EEEEEECCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCEEECCHHHHHHHHCCCCC
QAILVPQKAITRNSKGQAVAMVVNDQGLVELKIVTTAEVIDNQWRVTSGLAVGDKLIVEG
CEEEECHHHHCCCCCCCEEEEEECCCCEEEEEEEEEHHHHCCCEEEECCCCCCHHHHHHH
LQKIRPGAPVTTKVISETQAQ
HHHHCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MRQTIKLASVISVALWMAACSPQEEKAQAGAGPRSMEVGILNVVAQPQVIQVELPGRSKA
CCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEEECCCEEEEEECCCCCCE
FLEAEVRPQVSGIITKRSFVEGGNVKQGESLYQIDSATYKAALVSANADLARANAGLASA
EEEEECCCCHHHEEEHHHCCCCCCCCCCCCEEEECCCCEEEEEEECCCCHHHCCCCHHHH
KAKAARYQQLVKTNAISKQEFDEAEAAYKEALANVTVAEAAINTAKINLQYTEVLAPISG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHCEEEEEEEEHHHHHHCCCC
RIGKSSVTAGALVTANQSQTLATIQQLDPINVDIAQSSAQLLRLKAKLKQGKLQASENAD
CCCCCCCCCCEEEECCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCC
VQLLLEDGTQYSHPGKLQFAEVSVDPNTGSVILRAEFPNPEGILLPGMYVRAMLNAGTDP
EEEEEECCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCEEECCHHHHHHHHCCCCC
QAILVPQKAITRNSKGQAVAMVVNDQGLVELKIVTTAEVIDNQWRVTSGLAVGDKLIVEG
CEEEECHHHHCCCCCCCEEEEEECCCCEEEEEEEEEHHHHCCCEEEECCCCCCHHHHHHH
LQKIRPGAPVTTKVISETQAQ
HHHHCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]