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The map label for this gene is pleD [H]

Identifier: 160877438

GI number: 160877438

Start: 5121471

End: 5122403

Strand: Reverse

Name: pleD [H]

Synonym: Sbal195_4336

Alternate gene names: 160877438

Gene position: 5122403-5121471 (Counterclockwise)

Preceding gene: 160877439

Following gene: 160877437

Centisome position: 95.79

GC content: 45.98

Gene sequence:

>933_bases
ATGCATCTTGCAGAATTAATTGCTCGGCCCCAAAGTGAGAAAGGTAAGATCCTCATCGTTGATGATCAGCCGCTCAATAT
CAAAATACTGCATCAACTCTTCAATGAAGAGTATGAGTTGTTTATGGCGACCAATGGTGAGCAGGCGATAGCCATATGCC
AGAAGGTACAGCCAGATTTAGTGCTGTTAGACATTGAAATGCCTGGTATGTCGGGTTTTGACGTTTGCCAACATATTAAA
GCCGATCCTGAAACCGCGACTATTGGGGTTATTTTTGTCACGGCTCATTTTGATGAAGTGCAAGAAGTTAAAGGTTTTCA
GCTGGGTGCAGTCGATTTTATCCATAAACCTATCAATCCAATTATCACCACTGCGCGGGTAAAGAATCAGTTTACCCTCA
AGCGACAATCCGATCTGTTGCATTCCATCGCGCTGCTTGACAGTTTAACGGGCGTCGCCAATCGACGTCAGTTTGAGCAG
CGACTTCCTGAAATATGGAAACATTGCTGCCGCAACGAGTTAGCCTTATCTGTGGTGATGCTAGATGTGGATTTTTTTAA
ACGTTTTAATGATCGTTATGGTCATCAGGAAGGTGACCAATGCTTGCGGCATGTTGCAAAAGCCATAAGCGATTCTCTGC
TGCGCGCGACTGACTTTGTTGCACGTTATGGCGGGGAGGAGTTTATTTGTATTCTGCCAGAAACCAAGCTTGCCGGTGCC
ATTCATACGGCACAGAAGATAGTAAATGCAGTACAAGCTTTGCATCTTGAACATCTCGAGTCGAGCTTCCAAGAAGTGAC
GATTAGTGCGGGTGTCGCGAGCGTGCTACCCAAAAGTGATTTGACTTGGCAAGCACTGATCGAAACAGCGGACCAGCAAT
TGTACTTGGCGAAAGAGAGTGGACGTAACCAAGTAGTGGGCCTAGGGCTTTAG

Upstream 100 bases:

>100_bases
CAGGCCATCCACAATGGTCCATATTGCATGGGCAAGTGCAAGCGCTCGATTTTAATCAGGCATTAGATACGCTACGGATT
ATCATGTTGGAGGAGGCGTA

Downstream 100 bases:

>100_bases
CCTGTGAGTTTGTATATGATTTTTAGCGTGCATTTTATGAAGTTGCCGCTGTTTTTTGCTCGGCATGTGGATAATAAAGA
GTTATGAAAGCGCATTTGAT

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MHLAELIARPQSEKGKILIVDDQPLNIKILHQLFNEEYELFMATNGEQAIAICQKVQPDLVLLDIEMPGMSGFDVCQHIK
ADPETATIGVIFVTAHFDEVQEVKGFQLGAVDFIHKPINPIITTARVKNQFTLKRQSDLLHSIALLDSLTGVANRRQFEQ
RLPEIWKHCCRNELALSVVMLDVDFFKRFNDRYGHQEGDQCLRHVAKAISDSLLRATDFVARYGGEEFICILPETKLAGA
IHTAQKIVNAVQALHLEHLESSFQEVTISAGVASVLPKSDLTWQALIETADQQLYLAKESGRNQVVGLGL

Sequences:

>Translated_310_residues
MHLAELIARPQSEKGKILIVDDQPLNIKILHQLFNEEYELFMATNGEQAIAICQKVQPDLVLLDIEMPGMSGFDVCQHIK
ADPETATIGVIFVTAHFDEVQEVKGFQLGAVDFIHKPINPIITTARVKNQFTLKRQSDLLHSIALLDSLTGVANRRQFEQ
RLPEIWKHCCRNELALSVVMLDVDFFKRFNDRYGHQEGDQCLRHVAKAISDSLLRATDFVARYGGEEFICILPETKLAGA
IHTAQKIVNAVQALHLEHLESSFQEVTISAGVASVLPKSDLTWQALIETADQQLYLAKESGRNQVVGLGL
>Mature_310_residues
MHLAELIARPQSEKGKILIVDDQPLNIKILHQLFNEEYELFMATNGEQAIAICQKVQPDLVLLDIEMPGMSGFDVCQHIK
ADPETATIGVIFVTAHFDEVQEVKGFQLGAVDFIHKPINPIITTARVKNQFTLKRQSDLLHSIALLDSLTGVANRRQFEQ
RLPEIWKHCCRNELALSVVMLDVDFFKRFNDRYGHQEGDQCLRHVAKAISDSLLRATDFVARYGGEEFICILPETKLAGA
IHTAQKIVNAVQALHLEHLESSFQEVTISAGVASVLPKSDLTWQALIETADQQLYLAKESGRNQVVGLGL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=37.1951219512195, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082007, Length=225, Percent_Identity=32.4444444444444, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=175, Percent_Identity=35.4285714285714, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=30.9090909090909, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=36.7469879518072, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=163, Percent_Identity=36.1963190184049, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=34.7826086956522, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=35.7954545454545, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787802, Length=185, Percent_Identity=30.8108108108108, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788394, Length=115, Percent_Identity=33.9130434782609, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790863, Length=114, Percent_Identity=34.2105263157895, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789809, Length=129, Percent_Identity=31.0077519379845, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787229, Length=125, Percent_Identity=32, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=29.1139240506329, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=169, Percent_Identity=29.585798816568, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082052, Length=134, Percent_Identity=32.8358208955224, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=112, Percent_Identity=29.4642857142857, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789149, Length=103, Percent_Identity=33.9805825242718, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34689; Mature: 34689

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLAELIARPQSEKGKILIVDDQPLNIKILHQLFNEEYELFMATNGEQAIAICQKVQPDL
CCHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCE
VLLDIEMPGMSGFDVCQHIKADPETATIGVIFVTAHFDEVQEVKGFQLGAVDFIHKPINP
EEEEEECCCCCHHHHHHHCCCCCCCEEEEEEEEEECHHHHHHHCCCCCCHHHHHHCCCHH
IITTARVKNQFTLKRQSDLLHSIALLDSLTGVANRRQFEQRLPEIWKHCCRNELALSVVM
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHE
LDVDFFKRFNDRYGHQEGDQCLRHVAKAISDSLLRATDFVARYGGEEFICILPETKLAGA
EEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
IHTAQKIVNAVQALHLEHLESSFQEVTISAGVASVLPKSDLTWQALIETADQQLYLAKES
HHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECC
GRNQVVGLGL
CCCCEEEECC
>Mature Secondary Structure
MHLAELIARPQSEKGKILIVDDQPLNIKILHQLFNEEYELFMATNGEQAIAICQKVQPDL
CCHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCE
VLLDIEMPGMSGFDVCQHIKADPETATIGVIFVTAHFDEVQEVKGFQLGAVDFIHKPINP
EEEEEECCCCCHHHHHHHCCCCCCCEEEEEEEEEECHHHHHHHCCCCCCHHHHHHCCCHH
IITTARVKNQFTLKRQSDLLHSIALLDSLTGVANRRQFEQRLPEIWKHCCRNELALSVVM
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHE
LDVDFFKRFNDRYGHQEGDQCLRHVAKAISDSLLRATDFVARYGGEEFICILPETKLAGA
EEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHH
IHTAQKIVNAVQALHLEHLESSFQEVTISAGVASVLPKSDLTWQALIETADQQLYLAKES
HHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECC
GRNQVVGLGL
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA