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The map label for this gene is mrcA [H]

Identifier: 160877310

GI number: 160877310

Start: 4971881

End: 4974349

Strand: Direct

Name: mrcA [H]

Synonym: Sbal195_4208

Alternate gene names: 160877310

Gene position: 4971881-4974349 (Clockwise)

Preceding gene: 160877300

Following gene: 160877316

Centisome position: 92.98

GC content: 47.95

Gene sequence:

>2469_bases
GTGGCAGCATACTTTTATGTTTTGCCGGATTTGCCGGATGTTTCAACACTAAAAAATGTGCAACTTCAAACACCTTTACG
CATCTACAGCAGTGACGGCAAACTTATTTCACAATTTGGTGAGAAACGTCGCATTCCATTGAAGTTAGACGAAGTGCCAA
AACCCCTACTTCAAGCGTTTATCGCAACTGAAGATGCGCGTTTTTACGAGCATGGTGGTATAGATCCTGTAGGTGTACTT
CGCGCCGCATTAGTGATGTTGACCACAGGTGAGAAAAAGCAAGGTGCCAGTACCATTACCATGCAGGTTGCGCGTAACTT
CTTCTTAACCCGCGATAAAACCATTATTCGTAAAGTGAAGGAGATTTTTATCTCCTATCACATTGAAGAATTACTCACCA
AAGACGAGATTTTAGAACTCTATGTGAACCGCATTTACTTAGGCCAACGCGCCTATGGTGTTGGTGCCGCTGCACAAGTT
TATTTCGGTAAAGATGTTAAAGACCTTACACTCTCAGAAATGAGTATGATCGCGGGCTTACCCAAAGCGCCTTCGACACT
CAACCCTGTGACCTCACCTTCTCGTGCCGTCGCTCGCCGTAATGTCGTCCTCATGCGGATGAATGAAGTAGGTTACATCA
GCCAAGCCGAATACCAAGCCGCACTACAAGAACCGCTGGTCGCTAAATACCACGGCGCCGAAATTGATCTCTATGCGCCG
TATATTTCTGAAATGGCACGAGATTATATGGTGCAAAAGTACGGAGAAGAAGAAGCTTATACCAATGGCTACAACGTCTA
CACAACCATTTCATCGGATATACAGCTGAAAGCTCAGCAAGCATTACGTAATAACGTTTATGCCTATGATGAGCGTCATG
GCTACCGTGGTCCTGCCGCCGTTCTGTGGACAGATGAAAAACCAGATCAAGCTCAAATCAAGGCTGAACTGGCCAAGGTG
GCTTCAATTCAAGAACTGCAACCCGCAGCAGTCATCAGCGTGGCAGATCAACAAGCCAGTGTCATGACCGCCAAGGGCGA
GACTAAAGTCATTACTTGGGATGGTTTGAAGTGGGCGCGTAAATTTATTACCGATAAGCGTCAAGGCAGTGTACCTAAAT
CAGCTTCTGACATGCTCAAGGTTGGTGAGCGTATTTGGATCCGTGATAACGGCCAATATTTGCAGTTATCGCAAGTACCA
GAAGTTGCCAGCGCGACTGTGTCACTCGACCCACAAAATGGTGCGATTCGTTCTTTAGTCGGCGGATATAGCTTTAGCCA
AAGTCAGTACAACCGTGTCACTCAAGCAAAACGCCAATTAGGTTCGAATATCAAACCTTTCATCTATGCTGGCGCATTAG
AGAAAGGCTTTACCTTAGCGACCCTCATCAACAATGCACCGATCAATAAACCCGATATCAGCCAAGGAACCGCATGGCGC
CCTAAAAACTCGCCTGATATCTATGGCGGCCCAACCCGTTTACGCGTGGGCTTAGCGCAATCAATCAACGTGATGTCTGT
GCGTGCAATGCGCCATGTTGGACTCGATGCCACCATTGCAGAATTGATTAAATTTGGTTTCGACCCTAATGACTTACCGC
GTAACGAGTCGATTGCATTAGGTTCGTCTTCAGTAACGCCATTACAAGTGGTCACGGCATTTAACGTGTTTGCTAACGGC
GGTTTCTTACTCGAACCTTTCTATATCGATCGCGTCGAAGATGCCTATGGTAATGTGATTGAAAAAGCGAATCCAACATT
GGCCTGTAAGCCTAACCAAAGTCTTAGCCAACCAGTTACACCGAGCGATCCTATGGCGTACAGTGTTAGTGGAGACTCTA
GTGCTCAACCGAGTGCACCTGTTGCCCAGTGTTTCGAGCAAACGGGTCGCTTTGCGCCACAGATCATTTCAGAGCAAACG
GCATTCCTCATCACCGAGGCGTTAAAGAGCGTGATCTGGGGTGGCGGTGATTGGAGTCACGGCACTGGCTGGCAAGGTAC
GGCTTGGCGTGCTGCAAGATTGATTAAACGCCATGATATTGCGGGTAAAACAGGTACAACCAACGAATCTCGCGATACGT
GGTTCAGTGGATTTAGCCCTGTGCTCACCAGTACGTTCTGGGTCGGCTTCGACGATCACGGTCGACAACTGGGCAGAACT
GCATGGAATGCCAGCGCAGAGAAAGATCAGATCACCGGCGCCGAAGCCGGCGCGAAAACGGCAGGTCCGGGTTGGAACGA
GTTTATGAACAGTGTCTTACAAGGCACACCTGAAACCACATCGATTCCACCAGAAGGCATTGTCTCGGTACGGATCGATT
TAGCAACGGGTAAACTCACGCGTAAAACTGACTATACCAGCGCATTCGAATACTTCATCTCGGGCACTGAGCCGAAGGAA
TACATCAGCGAAACTCAGGATGAGAACAACATCTTCATCGATGCGCCAACTGACGATCTATTCCAATAA

Upstream 100 bases:

>100_bases
TGTCGCACTTAACGGAACATTAGGATAAATTTTGGTGAAGTGGTTAAAACGTATTGTTATAGCACTATTCAGTCTAGCCC
TTTTGGGAGTAGGCGCCATT

Downstream 100 bases:

>100_bases
ACGACTCATAGAAAGTCATTGCCCATACGGATAATGACCCAATAAAAAACGCGACTTAACGTCGCGTTTTTTATTGGCTT
GTTGATTTGAACAACTTGCC

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 822; Mature: 821

Protein sequence:

>822_residues
MAAYFYVLPDLPDVSTLKNVQLQTPLRIYSSDGKLISQFGEKRRIPLKLDEVPKPLLQAFIATEDARFYEHGGIDPVGVL
RAALVMLTTGEKKQGASTITMQVARNFFLTRDKTIIRKVKEIFISYHIEELLTKDEILELYVNRIYLGQRAYGVGAAAQV
YFGKDVKDLTLSEMSMIAGLPKAPSTLNPVTSPSRAVARRNVVLMRMNEVGYISQAEYQAALQEPLVAKYHGAEIDLYAP
YISEMARDYMVQKYGEEEAYTNGYNVYTTISSDIQLKAQQALRNNVYAYDERHGYRGPAAVLWTDEKPDQAQIKAELAKV
ASIQELQPAAVISVADQQASVMTAKGETKVITWDGLKWARKFITDKRQGSVPKSASDMLKVGERIWIRDNGQYLQLSQVP
EVASATVSLDPQNGAIRSLVGGYSFSQSQYNRVTQAKRQLGSNIKPFIYAGALEKGFTLATLINNAPINKPDISQGTAWR
PKNSPDIYGGPTRLRVGLAQSINVMSVRAMRHVGLDATIAELIKFGFDPNDLPRNESIALGSSSVTPLQVVTAFNVFANG
GFLLEPFYIDRVEDAYGNVIEKANPTLACKPNQSLSQPVTPSDPMAYSVSGDSSAQPSAPVAQCFEQTGRFAPQIISEQT
AFLITEALKSVIWGGGDWSHGTGWQGTAWRAARLIKRHDIAGKTGTTNESRDTWFSGFSPVLTSTFWVGFDDHGRQLGRT
AWNASAEKDQITGAEAGAKTAGPGWNEFMNSVLQGTPETTSIPPEGIVSVRIDLATGKLTRKTDYTSAFEYFISGTEPKE
YISETQDENNIFIDAPTDDLFQ

Sequences:

>Translated_822_residues
MAAYFYVLPDLPDVSTLKNVQLQTPLRIYSSDGKLISQFGEKRRIPLKLDEVPKPLLQAFIATEDARFYEHGGIDPVGVL
RAALVMLTTGEKKQGASTITMQVARNFFLTRDKTIIRKVKEIFISYHIEELLTKDEILELYVNRIYLGQRAYGVGAAAQV
YFGKDVKDLTLSEMSMIAGLPKAPSTLNPVTSPSRAVARRNVVLMRMNEVGYISQAEYQAALQEPLVAKYHGAEIDLYAP
YISEMARDYMVQKYGEEEAYTNGYNVYTTISSDIQLKAQQALRNNVYAYDERHGYRGPAAVLWTDEKPDQAQIKAELAKV
ASIQELQPAAVISVADQQASVMTAKGETKVITWDGLKWARKFITDKRQGSVPKSASDMLKVGERIWIRDNGQYLQLSQVP
EVASATVSLDPQNGAIRSLVGGYSFSQSQYNRVTQAKRQLGSNIKPFIYAGALEKGFTLATLINNAPINKPDISQGTAWR
PKNSPDIYGGPTRLRVGLAQSINVMSVRAMRHVGLDATIAELIKFGFDPNDLPRNESIALGSSSVTPLQVVTAFNVFANG
GFLLEPFYIDRVEDAYGNVIEKANPTLACKPNQSLSQPVTPSDPMAYSVSGDSSAQPSAPVAQCFEQTGRFAPQIISEQT
AFLITEALKSVIWGGGDWSHGTGWQGTAWRAARLIKRHDIAGKTGTTNESRDTWFSGFSPVLTSTFWVGFDDHGRQLGRT
AWNASAEKDQITGAEAGAKTAGPGWNEFMNSVLQGTPETTSIPPEGIVSVRIDLATGKLTRKTDYTSAFEYFISGTEPKE
YISETQDENNIFIDAPTDDLFQ
>Mature_821_residues
AAYFYVLPDLPDVSTLKNVQLQTPLRIYSSDGKLISQFGEKRRIPLKLDEVPKPLLQAFIATEDARFYEHGGIDPVGVLR
AALVMLTTGEKKQGASTITMQVARNFFLTRDKTIIRKVKEIFISYHIEELLTKDEILELYVNRIYLGQRAYGVGAAAQVY
FGKDVKDLTLSEMSMIAGLPKAPSTLNPVTSPSRAVARRNVVLMRMNEVGYISQAEYQAALQEPLVAKYHGAEIDLYAPY
ISEMARDYMVQKYGEEEAYTNGYNVYTTISSDIQLKAQQALRNNVYAYDERHGYRGPAAVLWTDEKPDQAQIKAELAKVA
SIQELQPAAVISVADQQASVMTAKGETKVITWDGLKWARKFITDKRQGSVPKSASDMLKVGERIWIRDNGQYLQLSQVPE
VASATVSLDPQNGAIRSLVGGYSFSQSQYNRVTQAKRQLGSNIKPFIYAGALEKGFTLATLINNAPINKPDISQGTAWRP
KNSPDIYGGPTRLRVGLAQSINVMSVRAMRHVGLDATIAELIKFGFDPNDLPRNESIALGSSSVTPLQVVTAFNVFANGG
FLLEPFYIDRVEDAYGNVIEKANPTLACKPNQSLSQPVTPSDPMAYSVSGDSSAQPSAPVAQCFEQTGRFAPQIISEQTA
FLITEALKSVIWGGGDWSHGTGWQGTAWRAARLIKRHDIAGKTGTTNESRDTWFSGFSPVLTSTFWVGFDDHGRQLGRTA
WNASAEKDQITGAEAGAKTAGPGWNEFMNSVLQGTPETTSIPPEGIVSVRIDLATGKLTRKTDYTSAFEYFISGTEPKEY
ISETQDENNIFIDAPTDDLFQ

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=818, Percent_Identity=51.5892420537897, Blast_Score=833, Evalue=0.0,
Organism=Escherichia coli, GI1788867, Length=260, Percent_Identity=33.8461538461538, Blast_Score=133, Evalue=5e-32,
Organism=Escherichia coli, GI1786343, Length=275, Percent_Identity=31.6363636363636, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1789601, Length=166, Percent_Identity=34.3373493975904, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 90536; Mature: 90405

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAYFYVLPDLPDVSTLKNVQLQTPLRIYSSDGKLISQFGEKRRIPLKLDEVPKPLLQAF
CCEEEEECCCCCCHHHHCCCEECCCEEEECCCCHHHHHHHHCCCCCEEHHHCCHHHHHHH
IATEDARFYEHGGIDPVGVLRAALVMLTTGEKKQGASTITMQVARNFFLTRDKTIIRKVK
HHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
EIFISYHIEELLTKDEILELYVNRIYLGQRAYGVGAAAQVYFGKDVKDLTLSEMSMIAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
PKAPSTLNPVTSPSRAVARRNVVLMRMNEVGYISQAEYQAALQEPLVAKYHGAEIDLYAP
CCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCHHHCCCCEEEEECH
YISEMARDYMVQKYGEEEAYTNGYNVYTTISSDIQLKAQQALRNNVYAYDERHGYRGPAA
HHHHHHHHHHHHHCCCCHHCCCCCEEEEEECCCHHHHHHHHHHCCCEEEECCCCCCCCEE
VLWTDEKPDQAQIKAELAKVASIQELQPAAVISVADQQASVMTAKGETKVITWDGLKWAR
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCEEEEEECCHHHHH
KFITDKRQGSVPKSASDMLKVGERIWIRDNGQYLQLSQVPEVASATVSLDPQNGAIRSLV
HHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCEEEECCCCCHHHCEEEECCCCCHHHHHH
GGYSFSQSQYNRVTQAKRQLGSNIKPFIYAGALEKGFTLATLINNAPINKPDISQGTAWR
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCC
PKNSPDIYGGPTRLRVGLAQSINVMSVRAMRHVGLDATIAELIKFGFDPNDLPRNESIAL
CCCCCCCCCCCHHEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEE
GSSSVTPLQVVTAFNVFANGGFLLEPFYIDRVEDAYGNVIEKANPTLACKPNQSLSQPVT
CCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
PSDPMAYSVSGDSSAQPSAPVAQCFEQTGRFAPQIISEQTAFLITEALKSVIWGGGDWSH
CCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GTGWQGTAWRAARLIKRHDIAGKTGTTNESRDTWFSGFSPVLTSTFWVGFDDHGRQLGRT
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHHHHEEECCCHHHHHHHHH
AWNASAEKDQITGAEAGAKTAGPGWNEFMNSVLQGTPETTSIPPEGIVSVRIDLATGKLT
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCEE
RKTDYTSAFEYFISGTEPKEYISETQDENNIFIDAPTDDLFQ
CCCCHHHHHHHHHCCCCHHHHHHHCCCCCCEEEECCCHHHCC
>Mature Secondary Structure 
AAYFYVLPDLPDVSTLKNVQLQTPLRIYSSDGKLISQFGEKRRIPLKLDEVPKPLLQAF
CEEEEECCCCCCHHHHCCCEECCCEEEECCCCHHHHHHHHCCCCCEEHHHCCHHHHHHH
IATEDARFYEHGGIDPVGVLRAALVMLTTGEKKQGASTITMQVARNFFLTRDKTIIRKVK
HHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHH
EIFISYHIEELLTKDEILELYVNRIYLGQRAYGVGAAAQVYFGKDVKDLTLSEMSMIAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
PKAPSTLNPVTSPSRAVARRNVVLMRMNEVGYISQAEYQAALQEPLVAKYHGAEIDLYAP
CCCCCCCCCCCCCHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCHHHCCCCEEEEECH
YISEMARDYMVQKYGEEEAYTNGYNVYTTISSDIQLKAQQALRNNVYAYDERHGYRGPAA
HHHHHHHHHHHHHCCCCHHCCCCCEEEEEECCCHHHHHHHHHHCCCEEEECCCCCCCCEE
VLWTDEKPDQAQIKAELAKVASIQELQPAAVISVADQQASVMTAKGETKVITWDGLKWAR
EEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCEEEEEECCHHHHH
KFITDKRQGSVPKSASDMLKVGERIWIRDNGQYLQLSQVPEVASATVSLDPQNGAIRSLV
HHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCEEEECCCCCHHHCEEEECCCCCHHHHHH
GGYSFSQSQYNRVTQAKRQLGSNIKPFIYAGALEKGFTLATLINNAPINKPDISQGTAWR
CCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCC
PKNSPDIYGGPTRLRVGLAQSINVMSVRAMRHVGLDATIAELIKFGFDPNDLPRNESIAL
CCCCCCCCCCCHHEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEE
GSSSVTPLQVVTAFNVFANGGFLLEPFYIDRVEDAYGNVIEKANPTLACKPNQSLSQPVT
CCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCC
PSDPMAYSVSGDSSAQPSAPVAQCFEQTGRFAPQIISEQTAFLITEALKSVIWGGGDWSH
CCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GTGWQGTAWRAARLIKRHDIAGKTGTTNESRDTWFSGFSPVLTSTFWVGFDDHGRQLGRT
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHHHHHEEECCCHHHHHHHHH
AWNASAEKDQITGAEAGAKTAGPGWNEFMNSVLQGTPETTSIPPEGIVSVRIDLATGKLT
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCEE
RKTDYTSAFEYFISGTEPKEYISETQDENNIFIDAPTDDLFQ
CCCCHHHHHHHHHCCCCHHHHHHHCCCCCCEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]