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The map label for this gene is gcvA [H]

Identifier: 160877295

GI number: 160877295

Start: 4958107

End: 4958979

Strand: Direct

Name: gcvA [H]

Synonym: Sbal195_4192

Alternate gene names: 160877295

Gene position: 4958107-4958979 (Clockwise)

Preceding gene: 160877293

Following gene: 160877299

Centisome position: 92.72

GC content: 49.14

Gene sequence:

>873_bases
ATGAGAAAGCTACCACCATTACGCGCTCTGCAAGTGTTTGAGGCCGCCGCCAGACAGCAGCATTTTTCTAAGGCAGCCGA
TGAACTGTGCATCACCCAAAGTGCGGTTTCTCACCAAGTACGAGTATTAGAAGAGTATTTTGGCGAGCAACTGTTTGACC
GCCAGGGGCGTAAACTGCAGTTAACCCCGAAGGGACACGGACTATTTCTAGAGTTAGAGCGGATATTCAACGAGTTAAGC
GACCTCAACTTGAAAGTACGCGACACACCCAATCAGCAGCTGTGCTTAGCAGTTTACAGTTCCTTCTCGGTGAAATGGTT
GATCCCCAGACTCGCCGATTTTCGCCGCCAATATCCGAGCGTAAAAATCCGCCTTGAGATGATCACCCAAGATCCTGATC
TCAGCACTAGCACGGCAGATATCTTTATCGCAGGAAAACTGAATCAACGCGGCTATTGGCAACAGTTACTACACAAAGAA
CGGCTAATCCCAGTGTGCAGCCCAAATTTCTACCTTAAATGGCGCGACATAAGCCTGAATAATTTCCAGCAACAAGCCCT
GCTGGTTGTCGATGAAGGCCCACTCGGACTCGACTGGCACAAGTGGTCATCGGCGCACCAAATCCCACTCGATAACGAAC
AACAGCATGTGTTTAGTCATATTGTGATGGCAATTGAGGCGGCGATTGCAGGCCAAGGCTTAGCCCTAGCGCCGGACTTT
ATGGTGGCTGGCGATATCACCACAGGGCGTTTAGTTGCTTTGAATCTTCCCGATGTGCATACTGGATTTGAATTTACTTT
CTTTTGTAAGCAACGCCGTTTATCAGAACCTGCGATTGCCGCCTTCTCCTCTTGGCTAAAATCACAGGCCTAA

Upstream 100 bases:

>100_bases
CAATGGCGGCATGATAATCCTCCCAAAACTTAAGCTGAAATGATGCTTTCTCATGCTAGATGAATTAAATTCATACTAAA
GCCTTTAGATATTGAAAATA

Downstream 100 bases:

>100_bases
GGTGAACGTCCAGACACAAAAAAGCCGACGCTGACGTCGGCTTAATTTATCAATATCGATTGATTACACTAACAAACCAA
TCTTCTCGTACACTTTCTTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRKLPPLRALQVFEAAARQQHFSKAADELCITQSAVSHQVRVLEEYFGEQLFDRQGRKLQLTPKGHGLFLELERIFNELS
DLNLKVRDTPNQQLCLAVYSSFSVKWLIPRLADFRRQYPSVKIRLEMITQDPDLSTSTADIFIAGKLNQRGYWQQLLHKE
RLIPVCSPNFYLKWRDISLNNFQQQALLVVDEGPLGLDWHKWSSAHQIPLDNEQQHVFSHIVMAIEAAIAGQGLALAPDF
MVAGDITTGRLVALNLPDVHTGFEFTFFCKQRRLSEPAIAAFSSWLKSQA

Sequences:

>Translated_290_residues
MRKLPPLRALQVFEAAARQQHFSKAADELCITQSAVSHQVRVLEEYFGEQLFDRQGRKLQLTPKGHGLFLELERIFNELS
DLNLKVRDTPNQQLCLAVYSSFSVKWLIPRLADFRRQYPSVKIRLEMITQDPDLSTSTADIFIAGKLNQRGYWQQLLHKE
RLIPVCSPNFYLKWRDISLNNFQQQALLVVDEGPLGLDWHKWSSAHQIPLDNEQQHVFSHIVMAIEAAIAGQGLALAPDF
MVAGDITTGRLVALNLPDVHTGFEFTFFCKQRRLSEPAIAAFSSWLKSQA
>Mature_290_residues
MRKLPPLRALQVFEAAARQQHFSKAADELCITQSAVSHQVRVLEEYFGEQLFDRQGRKLQLTPKGHGLFLELERIFNELS
DLNLKVRDTPNQQLCLAVYSSFSVKWLIPRLADFRRQYPSVKIRLEMITQDPDLSTSTADIFIAGKLNQRGYWQQLLHKE
RLIPVCSPNFYLKWRDISLNNFQQQALLVVDEGPLGLDWHKWSSAHQIPLDNEQQHVFSHIVMAIEAAIAGQGLALAPDF
MVAGDITTGRLVALNLPDVHTGFEFTFFCKQRRLSEPAIAAFSSWLKSQA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=34.5890410958904, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=29.5138888888889, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=31.1258278145695, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790208, Length=295, Percent_Identity=27.4576271186441, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786401, Length=256, Percent_Identity=25.390625, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=23.8255033557047, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=127, Percent_Identity=33.8582677165354, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=25.2595155709343, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33142; Mature: 33142

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLPPLRALQVFEAAARQQHFSKAADELCITQSAVSHQVRVLEEYFGEQLFDRQGRKLQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTPKGHGLFLELERIFNELSDLNLKVRDTPNQQLCLAVYSSFSVKWLIPRLADFRRQYPS
ECCCCCCEEEEHHHHHHHHHHCEEEEECCCCHHHHHHHHHCCCEEEHHHHHHHHHHHCCC
VKIRLEMITQDPDLSTSTADIFIAGKLNQRGYWQQLLHKERLIPVCSPNFYLKWRDISLN
EEEEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEECCCEEEEEEECCCC
NFQQQALLVVDEGPLGLDWHKWSSAHQIPLDNEQQHVFSHIVMAIEAAIAGQGLALAPDF
CCCCEEEEEECCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCE
MVAGDITTGRLVALNLPDVHTGFEFTFFCKQRRLSEPAIAAFSSWLKSQA
EEECCCCCCEEEEEECCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLPPLRALQVFEAAARQQHFSKAADELCITQSAVSHQVRVLEEYFGEQLFDRQGRKLQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTPKGHGLFLELERIFNELSDLNLKVRDTPNQQLCLAVYSSFSVKWLIPRLADFRRQYPS
ECCCCCCEEEEHHHHHHHHHHCEEEEECCCCHHHHHHHHHCCCEEEHHHHHHHHHHHCCC
VKIRLEMITQDPDLSTSTADIFIAGKLNQRGYWQQLLHKERLIPVCSPNFYLKWRDISLN
EEEEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEECCCEEEEEEECCCC
NFQQQALLVVDEGPLGLDWHKWSSAHQIPLDNEQQHVFSHIVMAIEAAIAGQGLALAPDF
CCCCEEEEEECCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCE
MVAGDITTGRLVALNLPDVHTGFEFTFFCKQRRLSEPAIAAFSSWLKSQA
EEECCCCCCEEEEEECCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]