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The map label for this gene is mrcB [H]

Identifier: 160876972

GI number: 160876972

Start: 4556620

End: 4558944

Strand: Reverse

Name: mrcB [H]

Synonym: Sbal195_3868

Alternate gene names: 160876972

Gene position: 4558944-4556620 (Counterclockwise)

Preceding gene: 160876973

Following gene: 160876971

Centisome position: 85.26

GC content: 50.67

Gene sequence:

>2325_bases
ATGAATGATGAAGTAGATATGACGACTAAGACGACAAAAAAAACACCCGCTTCCCGTAGGGCCAACAATGGCCGCGGTTT
ATTTCGACGAATGTGGTCAATCACTTGGAAATTAGCCTTAGCCGGATTAGCTGTAGTGACGTTCTACAGCATTTATCTCG
ATCAAATTATTGCGCAAAAATTTGAAGGCCAAAAATGGCACTTGCCCGCGCAGGTGTTCAGCCGTTCTATGGCGCTGTAT
CCCGGCGCGGCGGTCAGTCATCCGCAAATGATGGCTGAGCTTAAGTTGCTCGGCTATCGCAAAGTCGCGAATCCACGCCA
AGTGGGAGAATTTTCGGCATCGAGCACTCGTATCGAATTGTGGCGTCGACCATTTTTACATCCTGAAGGCGATCAAGTCG
AACAAAGAGTCATGATCAGCTTCGACAGCAATGGCGTCAGTTCAGTCTCCCGAGTGTCGGATAAGCGCCAACTTGCCGTA
TTCCATCTTGAGCCTGTGTTGCTCGATCGCATTATTACTGGCGACGGTGAAGACAGGCTGTTTGTGCCGACTGAAGAAAT
GCCTAAGTCGATTGTGCAGGCGTTATTGCTGGTGGAAGACCGCAGCTTTTACGAACATCATGGGGTGAATCCCTTCGCGA
TTGCGCGCGCCGCGTTTGTAAACATTAGCGCCGGTCGCACAGTGCAAGGTGGCTCGACGTTAACCCAGCAGTTAGCGAAA
AACTTTTTCCTATCGAGTGAGCGTTCTTTACTGCGTAAGGTGCGCGAAGCCTTGATGGCAGTGATTATCGATTTTCGTTA
CAGCAAGGACGAAATCCTCGAAGCCTATCTTAACGAAGTGTATATGGGGCAGGATAAATCCCGCGCCGTTCATGGTATGG
GTTTGGCATCGCGTTTCTATTTCGGCCGTCCTATCAGTGAGTTGACTATCGCCCAGCAGGCATTTTTAGTGGCTGCGATT
AAGGGACCGTCTTACTACAATCCATGGCGATATCCGGATCGCATTCAAGAGCGTCGCGATCTGGTATTGCGATTACTCAT
GGATGCGGGCGATTTAACCACGGCCGAGTACAAGGCGGCGGTAGAGTCACCTCTGGCGATCCGTAATCTTAATAAGCCAG
CACACCAGAAATTACCTTCTTTCTTCGCCCTGGTAAAACAGGAAGTGAACGAGCGTTATGGCGATGCGTTACTGAAGCAA
TCTGGGGTAAAAATCTATACCACGCTCGATCCTATGGCCCAAGAAGCCGCCGAAATTGCGGTGACACAAACCCTTAAAAG
CTTAGATAAGAGCAACAAGTCATTGCAGGTAGGCATGGTGGTTACCGACAAATACACGGGCGGTATCGCGGCAATGGTCG
GCGATAAAACCCCTGGATTTGATGGTTTTAACCGCGCGGTTGAAATTCGTCGTCCAATTGGCTCGTTAATTAAGCCCTTT
GTGTACGCCACGGCACTGGCACCCAATAGCCAATTTACCTTAGCGACTCCCCTTAAAGATCAGCCCATTACCTTAAAGAG
TGAGCAAGGTAAGACATGGTCACCGCAAAACTTCGATAAGAAGTTCAGCGGCCAAGTGCCTTTGTTAACGGCGCTGAAAA
AGTCGATGAACGTCCCGACAGTGAATCTTGGGATTGCGGTGGGCGTGGATGCCGTCGCGACGACTTTGGCTAAGTCGGGC
TGGCAGGAGCCGTTAAACGAGTATCCTTCTATGTTACTTGGCGCCGTCAATGGCTCGCCATTAATGGTGGCGCAGGTGTA
CCAAACCTTAGCCGATAGCGGGGTTTACCGTAAATTAACCACAGTGACGGCTGTGTTGGATGCTGATAAAAATCCATTAC
CAGTGACGCGATTGCCTAAAGAGCAGGCCATCTCACCCGATACTGACTTTTTGATCCAGTACGCGATGCAGCAAGTGGTC
AATACGGGCACAGCGACGCGCTTAGGCAATGCCTTCCCAGGCGTTGGGCTGGCGGGTAAAACAGGTACAAGTAACGACAG
CCGCGACTCTTGGTTTGCGGGCTTTGATGAGCGTAACGTTGCCGCTATTTGGGTGGGGCGTGATGATAACGGCAAAACCA
GCTTGTATGGCAGCAGTGGCGCCATGGCCGTGTATCTTGCCTTCTTGAAGGAACGTCCACCCATTAGTTTGCGTTCTATC
CAAGTGAATGGCGTCGTGAAGGGCTTTTTTGACCGAGATACCGGCGTGGCGAAGGAATCGGATTGCAGCAATGTTGATGC
GCTTCCTGCATTAACCGCGACTTATCGTCCGGTGAATAATTGCGGCGAGCCTTTGCAATGGTGGCAGAAAATACTCGGCC
AATAG

Upstream 100 bases:

>100_bases
CTCAGCCATAGCGCAGTGTTGCTGGTTTTAAATAGCGTGATAACACGCCTTGATTCGCACCTCGCTCACTGTTGTGCCTG
TGGCTGATGTCTTTAAATGC

Downstream 100 bases:

>100_bases
GCTCGATAATCGGCCAATCACGTACTTGTACTTTGAATATAAAATCTTAAGTAGTAGAACCTATGCTAATGGAAGTGGCG
TTATCGAGAGTACAGGCGTG

Product: penicillin-binding protein 1B

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 774; Mature: 774

Protein sequence:

>774_residues
MNDEVDMTTKTTKKTPASRRANNGRGLFRRMWSITWKLALAGLAVVTFYSIYLDQIIAQKFEGQKWHLPAQVFSRSMALY
PGAAVSHPQMMAELKLLGYRKVANPRQVGEFSASSTRIELWRRPFLHPEGDQVEQRVMISFDSNGVSSVSRVSDKRQLAV
FHLEPVLLDRIITGDGEDRLFVPTEEMPKSIVQALLLVEDRSFYEHHGVNPFAIARAAFVNISAGRTVQGGSTLTQQLAK
NFFLSSERSLLRKVREALMAVIIDFRYSKDEILEAYLNEVYMGQDKSRAVHGMGLASRFYFGRPISELTIAQQAFLVAAI
KGPSYYNPWRYPDRIQERRDLVLRLLMDAGDLTTAEYKAAVESPLAIRNLNKPAHQKLPSFFALVKQEVNERYGDALLKQ
SGVKIYTTLDPMAQEAAEIAVTQTLKSLDKSNKSLQVGMVVTDKYTGGIAAMVGDKTPGFDGFNRAVEIRRPIGSLIKPF
VYATALAPNSQFTLATPLKDQPITLKSEQGKTWSPQNFDKKFSGQVPLLTALKKSMNVPTVNLGIAVGVDAVATTLAKSG
WQEPLNEYPSMLLGAVNGSPLMVAQVYQTLADSGVYRKLTTVTAVLDADKNPLPVTRLPKEQAISPDTDFLIQYAMQQVV
NTGTATRLGNAFPGVGLAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTSLYGSSGAMAVYLAFLKERPPISLRSI
QVNGVVKGFFDRDTGVAKESDCSNVDALPALTATYRPVNNCGEPLQWWQKILGQ

Sequences:

>Translated_774_residues
MNDEVDMTTKTTKKTPASRRANNGRGLFRRMWSITWKLALAGLAVVTFYSIYLDQIIAQKFEGQKWHLPAQVFSRSMALY
PGAAVSHPQMMAELKLLGYRKVANPRQVGEFSASSTRIELWRRPFLHPEGDQVEQRVMISFDSNGVSSVSRVSDKRQLAV
FHLEPVLLDRIITGDGEDRLFVPTEEMPKSIVQALLLVEDRSFYEHHGVNPFAIARAAFVNISAGRTVQGGSTLTQQLAK
NFFLSSERSLLRKVREALMAVIIDFRYSKDEILEAYLNEVYMGQDKSRAVHGMGLASRFYFGRPISELTIAQQAFLVAAI
KGPSYYNPWRYPDRIQERRDLVLRLLMDAGDLTTAEYKAAVESPLAIRNLNKPAHQKLPSFFALVKQEVNERYGDALLKQ
SGVKIYTTLDPMAQEAAEIAVTQTLKSLDKSNKSLQVGMVVTDKYTGGIAAMVGDKTPGFDGFNRAVEIRRPIGSLIKPF
VYATALAPNSQFTLATPLKDQPITLKSEQGKTWSPQNFDKKFSGQVPLLTALKKSMNVPTVNLGIAVGVDAVATTLAKSG
WQEPLNEYPSMLLGAVNGSPLMVAQVYQTLADSGVYRKLTTVTAVLDADKNPLPVTRLPKEQAISPDTDFLIQYAMQQVV
NTGTATRLGNAFPGVGLAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTSLYGSSGAMAVYLAFLKERPPISLRSI
QVNGVVKGFFDRDTGVAKESDCSNVDALPALTATYRPVNNCGEPLQWWQKILGQ
>Mature_774_residues
MNDEVDMTTKTTKKTPASRRANNGRGLFRRMWSITWKLALAGLAVVTFYSIYLDQIIAQKFEGQKWHLPAQVFSRSMALY
PGAAVSHPQMMAELKLLGYRKVANPRQVGEFSASSTRIELWRRPFLHPEGDQVEQRVMISFDSNGVSSVSRVSDKRQLAV
FHLEPVLLDRIITGDGEDRLFVPTEEMPKSIVQALLLVEDRSFYEHHGVNPFAIARAAFVNISAGRTVQGGSTLTQQLAK
NFFLSSERSLLRKVREALMAVIIDFRYSKDEILEAYLNEVYMGQDKSRAVHGMGLASRFYFGRPISELTIAQQAFLVAAI
KGPSYYNPWRYPDRIQERRDLVLRLLMDAGDLTTAEYKAAVESPLAIRNLNKPAHQKLPSFFALVKQEVNERYGDALLKQ
SGVKIYTTLDPMAQEAAEIAVTQTLKSLDKSNKSLQVGMVVTDKYTGGIAAMVGDKTPGFDGFNRAVEIRRPIGSLIKPF
VYATALAPNSQFTLATPLKDQPITLKSEQGKTWSPQNFDKKFSGQVPLLTALKKSMNVPTVNLGIAVGVDAVATTLAKSG
WQEPLNEYPSMLLGAVNGSPLMVAQVYQTLADSGVYRKLTTVTAVLDADKNPLPVTRLPKEQAISPDTDFLIQYAMQQVV
NTGTATRLGNAFPGVGLAGKTGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTSLYGSSGAMAVYLAFLKERPPISLRSI
QVNGVVKGFFDRDTGVAKESDCSNVDALPALTATYRPVNNCGEPLQWWQKILGQ

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=702, Percent_Identity=43.5897435897436, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI87082258, Length=271, Percent_Identity=38.3763837638376, Blast_Score=182, Evalue=1e-46,
Organism=Escherichia coli, GI1788867, Length=531, Percent_Identity=30.3201506591337, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1789601, Length=170, Percent_Identity=31.1764705882353, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 85506; Mature: 85506

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDEVDMTTKTTKKTPASRRANNGRGLFRRMWSITWKLALAGLAVVTFYSIYLDQIIAQK
CCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEGQKWHLPAQVFSRSMALYPGAAVSHPQMMAELKLLGYRKVANPRQVGEFSASSTRIEL
HCCCCEECCHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHH
WRRPFLHPEGDQVEQRVMISFDSNGVSSVSRVSDKRQLAVFHLEPVLLDRIITGDGEDRL
HHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCEE
FVPTEEMPKSIVQALLLVEDRSFYEHHGVNPFAIARAAFVNISAGRTVQGGSTLTQQLAK
EECHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHEECCCCCEECCCHHHHHHHHH
NFFLSSERSLLRKVREALMAVIIDFRYSKDEILEAYLNEVYMGQDKSRAVHGMGLASRFY
HHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHH
FGRPISELTIAQQAFLVAAIKGPSYYNPWRYPDRIQERRDLVLRLLMDAGDLTTAEYKAA
CCCCHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VESPLAIRNLNKPAHQKLPSFFALVKQEVNERYGDALLKQSGVKIYTTLDPMAQEAAEIA
HHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
VTQTLKSLDKSNKSLQVGMVVTDKYTGGIAAMVGDKTPGFDGFNRAVEIRRPIGSLIKPF
HHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
VYATALAPNSQFTLATPLKDQPITLKSEQGKTWSPQNFDKKFSGQVPLLTALKKSMNVPT
HHHHHCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCCE
VNLGIAVGVDAVATTLAKSGWQEPLNEYPSMLLGAVNGSPLMVAQVYQTLADSGVYRKLT
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHH
TVTAVLDADKNPLPVTRLPKEQAISPDTDFLIQYAMQQVVNTGTATRLGNAFPGVGLAGK
HHHHHHCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCC
TGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTSLYGSSGAMAVYLAFLKERPPISLRSI
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEE
QVNGVVKGFFDRDTGVAKESDCSNVDALPALTATYRPVNNCGEPLQWWQKILGQ
EECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNDEVDMTTKTTKKTPASRRANNGRGLFRRMWSITWKLALAGLAVVTFYSIYLDQIIAQK
CCCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEGQKWHLPAQVFSRSMALYPGAAVSHPQMMAELKLLGYRKVANPRQVGEFSASSTRIEL
HCCCCEECCHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHH
WRRPFLHPEGDQVEQRVMISFDSNGVSSVSRVSDKRQLAVFHLEPVLLDRIITGDGEDRL
HHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCEE
FVPTEEMPKSIVQALLLVEDRSFYEHHGVNPFAIARAAFVNISAGRTVQGGSTLTQQLAK
EECHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHEECCCCCEECCCHHHHHHHHH
NFFLSSERSLLRKVREALMAVIIDFRYSKDEILEAYLNEVYMGQDKSRAVHGMGLASRFY
HHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHH
FGRPISELTIAQQAFLVAAIKGPSYYNPWRYPDRIQERRDLVLRLLMDAGDLTTAEYKAA
CCCCHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VESPLAIRNLNKPAHQKLPSFFALVKQEVNERYGDALLKQSGVKIYTTLDPMAQEAAEIA
HHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHH
VTQTLKSLDKSNKSLQVGMVVTDKYTGGIAAMVGDKTPGFDGFNRAVEIRRPIGSLIKPF
HHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
VYATALAPNSQFTLATPLKDQPITLKSEQGKTWSPQNFDKKFSGQVPLLTALKKSMNVPT
HHHHHCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCCCE
VNLGIAVGVDAVATTLAKSGWQEPLNEYPSMLLGAVNGSPLMVAQVYQTLADSGVYRKLT
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCHHHHHH
TVTAVLDADKNPLPVTRLPKEQAISPDTDFLIQYAMQQVVNTGTATRLGNAFPGVGLAGK
HHHHHHCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCC
TGTSNDSRDSWFAGFDERNVAAIWVGRDDNGKTSLYGSSGAMAVYLAFLKERPPISLRSI
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEE
QVNGVVKGFFDRDTGVAKESDCSNVDALPALTATYRPVNNCGEPLQWWQKILGQ
EECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]