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The map label for this gene is thrS [H]

Identifier: 160876899

GI number: 160876899

Start: 4472838

End: 4474034

Strand: Reverse

Name: thrS [H]

Synonym: Sbal195_3795

Alternate gene names: 160876899

Gene position: 4474034-4472838 (Counterclockwise)

Preceding gene: 160876900

Following gene: 160876896

Centisome position: 83.67

GC content: 45.78

Gene sequence:

>1197_bases
ATGAAAACACATCGCGAAATTGCAGAACAACTTGACCTATTTCACTCTGAACCACAGGCGCCGGGTATGATTTTTTGGCA
CCCGAATGGCTGGGCGTTATTCCGAGATATTCAAGAGCACATGAGGCGCGTGTATCGCCGAAATGGTTTTGAGGAAGTGA
ACACGCCTGTTTTTATGAAGAAGGAGTTATGGCAAACGTCTGGGCATATGGACATGTTTGCAGAGAACATGTTTTTCGGT
GGCGGAAAAGAAAACATGGGTGAATATGTTTTAAAACCTATGTCTTGTCCTGCCCATATTTTGCTGTTTAAGCGCGGGGT
ACATAGCCATAGAGATTTGCCACTGCGACTATTTGAGTTTGGCTTGGTGCACCGTAATGAATCCTCAGGCTCGTTAAATG
GTTGCCTGCGCGCACGCCAGTTCACTCAGGATGATGCCCATGTTTTTTGCTGTTGGTCTCAGGCAGAGGAGGAGATATGT
GGTTTTCTCCAACGGGCAAAGCAGGTTTATGGTGATTACGGCTACCATAATTTAGACGTAAAAATATCGACTAAACCTGA
GAAGGCATTAGGCAGCGATGAGCAATGGTGCCGAGCACAAAAGCTATTGGATTCAGCCTGCCAGATTGAGGGCTTTGATG
CCGAGTATCAAGCTGGCGAAGGGGCTTTTTATGGTCCCAAAATTGAATTGTCACTGACAGATTCTATGGGAAGACGCTGG
CAGTGTGGCACTATCCAGTTTGATTTCAATTTGCCTGAAAGATTCGATGTTCAGTACACAAATGAGCAAGGTGAATTCGA
ACGGCCAGTGATTATGCACCAAGCTATGTATGGCTCTATCGAGCGTTGGTTGGGGATTCTCTTGGAAGTCTCACAGGGTA
ACCTGCCGGATTGGTTGCATCCCGTGCCTGTTGCTATTGCCTCAGTGAATGAGTCAAACAGTCAATTTGTCCGTCAGTTA
GCCGCACTATGTTTAGCACGAGACATGCGAGTGTTGGTTGATATCAGTCAAAACTCAGTTGCCCGCAAAATGAAACGCTT
TCACAAGCAAAAAGTGCCTTACATCTTAACGGTTGGGGATAAGGAAGAAGCCGAGAAGTGTGTGTCAGTTAAGTGCCGCA
AAGATGGTGTTATGACTCACATCCCTCTTGCTGAATTAGCTGACTATCTTGCGGATAAACTAGTTCGAAAACAATAA

Upstream 100 bases:

>100_bases
CTTTAAAAAAACCCTCTGAGCGACATCGCCAGAGGGTTTTTTTATACCTTGGTTTTGTATTTCGCTCAGATTTTACTCAG
ATTTTACTCAGGAGAGAATG

Downstream 100 bases:

>100_bases
TATAAGGGGAATGGTGTTTGCTATTTAAGGCTAGCTAGGGTTCCCACGGCTAGCCTTTACTCATTTTATGATCCCGCTCT
TTGCGCTAAGGCTTAACGAT

Product: tRNA synthetase class II

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWALFRDIQEHMRRVYRRNGFEEVNTPVFMKKELWQTSGHMDMFAENMFFG
GGKENMGEYVLKPMSCPAHILLFKRGVHSHRDLPLRLFEFGLVHRNESSGSLNGCLRARQFTQDDAHVFCCWSQAEEEIC
GFLQRAKQVYGDYGYHNLDVKISTKPEKALGSDEQWCRAQKLLDSACQIEGFDAEYQAGEGAFYGPKIELSLTDSMGRRW
QCGTIQFDFNLPERFDVQYTNEQGEFERPVIMHQAMYGSIERWLGILLEVSQGNLPDWLHPVPVAIASVNESNSQFVRQL
AALCLARDMRVLVDISQNSVARKMKRFHKQKVPYILTVGDKEEAEKCVSVKCRKDGVMTHIPLAELADYLADKLVRKQ

Sequences:

>Translated_398_residues
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWALFRDIQEHMRRVYRRNGFEEVNTPVFMKKELWQTSGHMDMFAENMFFG
GGKENMGEYVLKPMSCPAHILLFKRGVHSHRDLPLRLFEFGLVHRNESSGSLNGCLRARQFTQDDAHVFCCWSQAEEEIC
GFLQRAKQVYGDYGYHNLDVKISTKPEKALGSDEQWCRAQKLLDSACQIEGFDAEYQAGEGAFYGPKIELSLTDSMGRRW
QCGTIQFDFNLPERFDVQYTNEQGEFERPVIMHQAMYGSIERWLGILLEVSQGNLPDWLHPVPVAIASVNESNSQFVRQL
AALCLARDMRVLVDISQNSVARKMKRFHKQKVPYILTVGDKEEAEKCVSVKCRKDGVMTHIPLAELADYLADKLVRKQ
>Mature_398_residues
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWALFRDIQEHMRRVYRRNGFEEVNTPVFMKKELWQTSGHMDMFAENMFFG
GGKENMGEYVLKPMSCPAHILLFKRGVHSHRDLPLRLFEFGLVHRNESSGSLNGCLRARQFTQDDAHVFCCWSQAEEEIC
GFLQRAKQVYGDYGYHNLDVKISTKPEKALGSDEQWCRAQKLLDSACQIEGFDAEYQAGEGAFYGPKIELSLTDSMGRRW
QCGTIQFDFNLPERFDVQYTNEQGEFERPVIMHQAMYGSIERWLGILLEVSQGNLPDWLHPVPVAIASVNESNSQFVRQL
AALCLARDMRVLVDISQNSVARKMKRFHKQKVPYILTVGDKEEAEKCVSVKCRKDGVMTHIPLAELADYLADKLVRKQ

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=393, Percent_Identity=37.6590330788804, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI38570111, Length=393, Percent_Identity=36.1323155216285, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI20070344, Length=408, Percent_Identity=35.0490196078431, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1788013, Length=393, Percent_Identity=41.7302798982188, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI71984184, Length=387, Percent_Identity=36.9509043927649, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI71984192, Length=387, Percent_Identity=36.9509043927649, Blast_Score=277, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6322112, Length=378, Percent_Identity=39.4179894179894, Blast_Score=284, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=35.0125944584383, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24583842, Length=391, Percent_Identity=38.1074168797954, Blast_Score=280, Evalue=9e-76,
Organism=Drosophila melanogaster, GI28574141, Length=391, Percent_Identity=38.1074168797954, Blast_Score=280, Evalue=9e-76,
Organism=Drosophila melanogaster, GI24583839, Length=391, Percent_Identity=38.1074168797954, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 45891; Mature: 45891

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWALFRDIQEHMRRVYRRNGFEEVNTPVFMK
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEH
KELWQTSGHMDMFAENMFFGGGKENMGEYVLKPMSCPAHILLFKRGVHSHRDLPLRLFEF
HHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHH
GLVHRNESSGSLNGCLRARQFTQDDAHVFCCWSQAEEEICGFLQRAKQVYGDYGYHNLDV
HHEECCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
KISTKPEKALGSDEQWCRAQKLLDSACQIEGFDAEYQAGEGAFYGPKIELSLTDSMGRRW
EEECCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCEEEEEEECCCCCEE
QCGTIQFDFNLPERFDVQYTNEQGEFERPVIMHQAMYGSIERWLGILLEVSQGNLPDWLH
ECEEEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
PVPVAIASVNESNSQFVRQLAALCLARDMRVLVDISQNSVARKMKRFHKQKVPYILTVGD
CCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECC
KEEAEKCVSVKCRKDGVMTHIPLAELADYLADKLVRKQ
HHHHHHHHHHEECCCCCEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTHREIAEQLDLFHSEPQAPGMIFWHPNGWALFRDIQEHMRRVYRRNGFEEVNTPVFMK
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEH
KELWQTSGHMDMFAENMFFGGGKENMGEYVLKPMSCPAHILLFKRGVHSHRDLPLRLFEF
HHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCHHHHHH
GLVHRNESSGSLNGCLRARQFTQDDAHVFCCWSQAEEEICGFLQRAKQVYGDYGYHNLDV
HHEECCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
KISTKPEKALGSDEQWCRAQKLLDSACQIEGFDAEYQAGEGAFYGPKIELSLTDSMGRRW
EEECCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCEEEEEEECCCCCEE
QCGTIQFDFNLPERFDVQYTNEQGEFERPVIMHQAMYGSIERWLGILLEVSQGNLPDWLH
ECEEEEEECCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCC
PVPVAIASVNESNSQFVRQLAALCLARDMRVLVDISQNSVARKMKRFHKQKVPYILTVGD
CCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCEEEEECC
KEEAEKCVSVKCRKDGVMTHIPLAELADYLADKLVRKQ
HHHHHHHHHHEECCCCCEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA