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The map label for this gene is yafC [C]

Identifier: 160876066

GI number: 160876066

Start: 3507810

End: 3508748

Strand: Direct

Name: yafC [C]

Synonym: Sbal195_2956

Alternate gene names: 160876066

Gene position: 3507810-3508748 (Clockwise)

Preceding gene: 160876063

Following gene: 160876068

Centisome position: 65.6

GC content: 44.2

Gene sequence:

>939_bases
ATGAATGATATAAACTCAATCAACATGCGAGCGTTAAAGTTTTTTATCGGCGTTTATGACAGCAAAAGCTATTCAGTTGT
GGCTCGAAAGGAAGGCGTCTCTCCCTCGATGATTTCCAGAGTCATCAGCCAACTAGAAGATTCTCTGGGACAGCAGCTGT
TTTATCGAAATACCAGGGCAATCATACCCACGGAATCGGGCAGGATTTTCATTGAATATGCGCGTTCCATGGCAGAGCAA
CTGAATGAAGTCCGCAATGAATTATTAGATAGGACGATAGAACCTAAGGGATTGATCAGAATTAATGCCCCGGTTTTTTT
CGGGCAGCGACATATTGCGCCTTGGTTAGCGGGGTTTTTAAGGCGATATCCGCAGGTACAAGTGGAACTCACACAAACAG
ACGATTATATCGATCCGCATGTTAGTGCCGCCGATATTATCTTTCGTATTGGCACTTTGGTTGATTCATCACTCCACTCA
AGAATACTGGGAAGCCAAACCTATCATCTTGCGGCGTCCAAAGCGTATATAGCACTGCATGGCATGCCCACTGAACCCGC
CGATCTAAAAAACCATCAATGCCTAGTCTATAGCGGATCTTCGGGTCAAAATCGCTGGTTTTTTAACAAGAACAAGACGA
CATGGCTGCATCAACCCATCACCCCAATCCTCACCTCAAATAATGCGGAAACATTACTCATAGCCGCATTAAATGATATG
GGAATCGTCCTCTTTCCTGACTGGTTAATCGGTGACTCTCTTAAAAATGGACAACTTATTTCATTACTCACGGACTGCGA
ATCAGCGATAAAAATTGATCAGCATTACATTACTGCCATTTATCCAAACTCCCGCACTCCCTCATTGAATGTCCGCGCCG
TAATTGACTACTTTCTTGAGGTGTATGGCAATCCGCCCTACTGGAAATATCAAGGTTAG

Upstream 100 bases:

>100_bases
GCATACATAAGCTCCTGTGATTAAGGGCAGCAAGAGTACCCTAGTCGACAAAACCAATAAATGAGACAATACAGGAAACA
CCTGTGCAGAATTTGCAAAA

Downstream 100 bases:

>100_bases
TGAATCGCTGTACTTAAGCTGCAACAAATACATTAAATCGGGCACTTAATGGGTGATCTAAATTATCGACCATAAGACTC
AAGGCATGAAACTCAGCCCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNDINSINMRALKFFIGVYDSKSYSVVARKEGVSPSMISRVISQLEDSLGQQLFYRNTRAIIPTESGRIFIEYARSMAEQ
LNEVRNELLDRTIEPKGLIRINAPVFFGQRHIAPWLAGFLRRYPQVQVELTQTDDYIDPHVSAADIIFRIGTLVDSSLHS
RILGSQTYHLAASKAYIALHGMPTEPADLKNHQCLVYSGSSGQNRWFFNKNKTTWLHQPITPILTSNNAETLLIAALNDM
GIVLFPDWLIGDSLKNGQLISLLTDCESAIKIDQHYITAIYPNSRTPSLNVRAVIDYFLEVYGNPPYWKYQG

Sequences:

>Translated_312_residues
MNDINSINMRALKFFIGVYDSKSYSVVARKEGVSPSMISRVISQLEDSLGQQLFYRNTRAIIPTESGRIFIEYARSMAEQ
LNEVRNELLDRTIEPKGLIRINAPVFFGQRHIAPWLAGFLRRYPQVQVELTQTDDYIDPHVSAADIIFRIGTLVDSSLHS
RILGSQTYHLAASKAYIALHGMPTEPADLKNHQCLVYSGSSGQNRWFFNKNKTTWLHQPITPILTSNNAETLLIAALNDM
GIVLFPDWLIGDSLKNGQLISLLTDCESAIKIDQHYITAIYPNSRTPSLNVRAVIDYFLEVYGNPPYWKYQG
>Mature_312_residues
MNDINSINMRALKFFIGVYDSKSYSVVARKEGVSPSMISRVISQLEDSLGQQLFYRNTRAIIPTESGRIFIEYARSMAEQ
LNEVRNELLDRTIEPKGLIRINAPVFFGQRHIAPWLAGFLRRYPQVQVELTQTDDYIDPHVSAADIIFRIGTLVDSSLHS
RILGSQTYHLAASKAYIALHGMPTEPADLKNHQCLVYSGSSGQNRWFFNKNKTTWLHQPITPILTSNNAETLLIAALNDM
GIVLFPDWLIGDSLKNGQLISLLTDCESAIKIDQHYITAIYPNSRTPSLNVRAVIDYFLEVYGNPPYWKYQG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=30.5084745762712, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=30.6397306397306, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=30.7167235494881, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87081978, Length=304, Percent_Identity=27.9605263157895, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=25.6055363321799, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=26.9360269360269, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=22.8187919463087, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787589, Length=255, Percent_Identity=24.3137254901961, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790208, Length=146, Percent_Identity=30.8219178082192, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786448, Length=298, Percent_Identity=23.1543624161074, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI157672245, Length=123, Percent_Identity=30.8943089430894, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35358; Mature: 35358

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50931 HTH_LYSR ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDINSINMRALKFFIGVYDSKSYSVVARKEGVSPSMISRVISQLEDSLGQQLFYRNTRA
CCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
IIPTESGRIFIEYARSMAEQLNEVRNELLDRTIEPKGLIRINAPVFFGQRHIAPWLAGFL
EEECCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECCHHHHHHHHHHH
RRYPQVQVELTQTDDYIDPHVSAADIIFRIGTLVDSSLHSRILGSQTYHLAASKAYIALH
HHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEE
GMPTEPADLKNHQCLVYSGSSGQNRWFFNKNKTTWLHQPITPILTSNNAETLLIAALNDM
CCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEEECCCCEEEECCCCCEEEEEEECCC
GIVLFPDWLIGDSLKNGQLISLLTDCESAIKIDQHYITAIYPNSRTPSLNVRAVIDYFLE
CEEEECCHHCCCCCCCCCEEEEHHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHHHH
VYGNPPYWKYQG
HCCCCCCEEECC
>Mature Secondary Structure
MNDINSINMRALKFFIGVYDSKSYSVVARKEGVSPSMISRVISQLEDSLGQQLFYRNTRA
CCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
IIPTESGRIFIEYARSMAEQLNEVRNELLDRTIEPKGLIRINAPVFFGQRHIAPWLAGFL
EEECCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECCHHHHHHHHHHH
RRYPQVQVELTQTDDYIDPHVSAADIIFRIGTLVDSSLHSRILGSQTYHLAASKAYIALH
HHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEE
GMPTEPADLKNHQCLVYSGSSGQNRWFFNKNKTTWLHQPITPILTSNNAETLLIAALNDM
CCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEEECCCCEEEECCCCCEEEEEEECCC
GIVLFPDWLIGDSLKNGQLISLLTDCESAIKIDQHYITAIYPNSRTPSLNVRAVIDYFLE
CEEEECCHHCCCCCCCCCEEEEHHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHHHH
VYGNPPYWKYQG
HCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]