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The map label for this gene is queA

Identifier: 160875993

GI number: 160875993

Start: 3435578

End: 3436615

Strand: Reverse

Name: queA

Synonym: Sbal195_2883

Alternate gene names: 160875993

Gene position: 3436615-3435578 (Counterclockwise)

Preceding gene: 160875994

Following gene: 160875992

Centisome position: 64.27

GC content: 49.81

Gene sequence:

>1038_bases
ATGCGCGTTGCAGACTTTTCTTTCGATCTCCCCGATGAGCTGATCGCTCGTTATCCTATGGCGCAGCGTAATGCATCACG
TCTGTTAACCTTAGACGGCAATTCTGGCGCGTTAGGCGACAAACAGTTTACCGATCTGCTGGGGATGATTAATCCCGGCG
ATTTAATGGTGTTCAACAATACCCGAGTGATCCCCGCGCGTATGTTTGGCCAAAAGGCCAGCGGCGGTAAGTTAGAAATC
TTAGTTGAGCGTATGCTCGATGATAAGCGCATTCTTGCTCACGTTCGCAGCTCTAAATCCCCAAAAGTCGATTCGTTAAT
TCTGCTCGACGGTGGCTATCAAATGAAGATGGTCGCCCGCCACGATACCTTGTTTGAACTTGAACTGTTATCCGAGTTGA
CCATTCTAGAGGTGCTCGAAGCCGTAGGGCATATGCCGCTACCGCCTTATATCGACAGACCCGATGAAGATGCTGACAAA
GAGCGCTATCAAACCGTGTATAACCAAAACCCAGGCGCCGTTGCTGCGCCAACGGCGGGATTACATTTTGACGATGCCAT
GCTCGATGCATTAAAAGCCAAGGGTGTGAATATCGCGTTTGTGACCTTGCATGTGGGCGCGGGAACGTTTCAACCGGTGC
GTGTTGATACCATTCTTGAGCACAAAATGCATTCAGAGTGGGCCAATGTGCCGCAGGATGTGGTCGATTTAATCGCGCAA
ACCAAAGCGGCGGGTAAACGCGTCGTGGCTGTTGGTACAACATCTGTACGTTCCTTAGAAAGTGCGGCACGCGCCAGCCA
AGGTGAACTCAAAGCCTTTAGCGGTGATACCGACATCTTTATTTATCCCGGTTATCAATTCCAAGTGGTCGATGCCATGG
TGACGAACTTCCACCTCCCTGAATCGACCCTCATCATGTTAGTCAGTGCATTTGCGGGTTTTGATCATGTTATGGCGGCG
TATCAACATGCTATCGCGCAAAAATATCGCTTCTTTAGCTATGGCGATGCCATGTTTGTGACGAAAAAAGCCCACTAG

Upstream 100 bases:

>100_bases
GGTTCCTGAGCAAGAAGTGGACGCTGGCAATAAGTAATCTTGATATTCTTGGCCTCTAACCATAGACTAGCCGCCGTTCT
AAGCTACCTAGAGGCCTATC

Downstream 100 bases:

>100_bases
ACCAAGATTTTGTTTTAAAATAGCCGCCCAAAGTGAGTATGGGCGGCAACTTTTTTTCGTCAAAAGGTTGAACCTTTTAA
CGCTGTCCCCATCTCTATTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLGMINPGDLMVFNNTRVIPARMFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKVDSLILLDGGYQMKMVARHDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLDALKAKGVNIAFVTLHVGAGTFQPVRVDTILEHKMHSEWANVPQDVVDLIAQ
TKAAGKRVVAVGTTSVRSLESAARASQGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLVSAFAGFDHVMAA
YQHAIAQKYRFFSYGDAMFVTKKAH

Sequences:

>Translated_345_residues
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLGMINPGDLMVFNNTRVIPARMFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKVDSLILLDGGYQMKMVARHDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLDALKAKGVNIAFVTLHVGAGTFQPVRVDTILEHKMHSEWANVPQDVVDLIAQ
TKAAGKRVVAVGTTSVRSLESAARASQGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLVSAFAGFDHVMAA
YQHAIAQKYRFFSYGDAMFVTKKAH
>Mature_345_residues
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLGMINPGDLMVFNNTRVIPARMFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKVDSLILLDGGYQMKMVARHDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLDALKAKGVNIAFVTLHVGAGTFQPVRVDTILEHKMHSEWANVPQDVVDLIAQ
TKAAGKRVVAVGTTSVRSLESAARASQGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLVSAFAGFDHVMAA
YQHAIAQKYRFFSYGDAMFVTKKAH

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=68.8953488372093, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_SHEB2 (B8EDJ6)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357497.1
- ProteinModelPortal:   B8EDJ6
- SMR:   B8EDJ6
- GeneID:   7088259
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_1569
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38064; Mature: 38064

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLGMINPGDLMVFNN
CEECCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCEEEECC
TRVIPARMFGQKASGGKLEILVERMLDDKRILAHVRSSKSPKVDSLILLDGGYQMKMVAR
CEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEC
HDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADKERYQTVYNQNPGAVAAPTAG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCC
LHFDDAMLDALKAKGVNIAFVTLHVGAGTFQPVRVDTILEHKMHSEWANVPQDVVDLIAQ
CCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHH
TKAAGKRVVAVGTTSVRSLESAARASQGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLP
HHHCCCEEEEEECHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEEHHHHHHHCCCC
ESTLIMLVSAFAGFDHVMAAYQHAIAQKYRFFSYGDAMFVTKKAH
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCEEEEEECCC
>Mature Secondary Structure
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLGMINPGDLMVFNN
CEECCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCEEEECC
TRVIPARMFGQKASGGKLEILVERMLDDKRILAHVRSSKSPKVDSLILLDGGYQMKMVAR
CEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEC
HDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADKERYQTVYNQNPGAVAAPTAG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCC
LHFDDAMLDALKAKGVNIAFVTLHVGAGTFQPVRVDTILEHKMHSEWANVPQDVVDLIAQ
CCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHH
TKAAGKRVVAVGTTSVRSLESAARASQGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLP
HHHCCCEEEEEECHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEEHHHHHHHCCCC
ESTLIMLVSAFAGFDHVMAAYQHAIAQKYRFFSYGDAMFVTKKAH
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA