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The map label for this gene is tgt [H]

Identifier: 160875992

GI number: 160875992

Start: 3434291

End: 3435415

Strand: Reverse

Name: tgt [H]

Synonym: Sbal195_2882

Alternate gene names: 160875992

Gene position: 3435415-3434291 (Counterclockwise)

Preceding gene: 160875993

Following gene: 160875991

Centisome position: 64.25

GC content: 46.58

Gene sequence:

>1125_bases
ATGAAATTTGAATTAGATACTACCGACGGCCGTGCGCGTCGTGGTCGGTTGATTTTTGACCGTGGCACAGTAGAAACCCC
AGCATTTATGCCTGTGGGAACCTACGGCACAGTTAAAGGTATGACGCCAGAAGAAGTACGTGCCACAGGCGCAGACATTC
TGCTCGGTAACACTTTCCATTTATGGTTGCGCCCAGGCGAAGAAATCATGCGCAAGCACGGTGACTTGCATGATTTTATG
AACTGGCAGCGTCCGATTTTAACTGACTCGGGTGGTTTCCAAGTATTCAGTCTTGGCGATATCCGTAAGATCACTGAAGA
AGGTGTGCATTTCCGTTCGCCAATCAATGGCGAGAAAATCTTCTTAGATCCTGAAAAATCAATGCAAATTCAAGATGCAT
TGGGCAGTGATGTAGTGATGATTTTTGACGAATGTACGCCATATCCCGCAACCGAAGACGAAGCGCGTAAGTCAATGCAG
ATGTCTCTGCGCTGGGCGAGACGTTCACGTGATGAATTTGACCGTTTAGAGAACCCTAACTCTCTGTTCGGTATTATTCA
GGGCGGTGTGTATGAAGATTTACGCGACGAAAGCTTAAAAGGCTTAGTCGATATTGGTTTCGACGGTTATGCCGTCGGTG
GTTTGGCCGTAGGAGAGCCTAAAGCGGATATGCATCGCATTCTTGAGCATATTTGCCCGCAAATTCCTGCAGACAAACCT
CGCTATCTGATGGGGGTCGGTAAACCAGAAGATTTAGTTGAAGGTGTACGTCGTGGTGTTGACATGTTTGACTGTGTGAT
GCCAACCCGTAATGCCCGCAACGGTCATCTGTTTACGAGTGAAGGTGTGATCAAAATACGCAATGCGCGTCATAGAGATG
ACACGTCACCACTTGATACTAAGTGTGATTGTTATACCTGTAAAAATTATTCACGGGCGTACCTTTACCACTTAGATCGT
TGTAATGAGATTCTGGGTGCGCGTTTAAACACCATTCACAACTTGAGATACTATCAAATGTTAATGGAAGGTTTGCGCGG
TGCGATTGAGACGGGTACATTAGACGCCTTTGTGGCGGACTTCTATACCAGTCAAGGTCGCGAAGTACCAGAGTTAGTCG
ATTGA

Upstream 100 bases:

>100_bases
AAAGGTTGAACCTTTTAACGCTGTCCCCATCTCTATTGTTAATCAAAATGGCATTAGCCATTAAAAATGTAGTCAGACTG
TTTATCTGGCGAGGTGAATT

Downstream 100 bases:

>100_bases
TTTTTTGCTAATAAGAAGAGAAAAATATGTTTATTTCAAATGCATACGCAAGCGCTGCCGGCGCACCACAAGGTGGCGGC
ACGATGGAATTGATTTTCAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKFELDTTDGRARRGRLIFDRGTVETPAFMPVGTYGTVKGMTPEEVRATGADILLGNTFHLWLRPGEEIMRKHGDLHDFM
NWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKIFLDPEKSMQIQDALGSDVVMIFDECTPYPATEDEARKSMQ
MSLRWARRSRDEFDRLENPNSLFGIIQGGVYEDLRDESLKGLVDIGFDGYAVGGLAVGEPKADMHRILEHICPQIPADKP
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDTKCDCYTCKNYSRAYLYHLDR
CNEILGARLNTIHNLRYYQMLMEGLRGAIETGTLDAFVADFYTSQGREVPELVD

Sequences:

>Translated_374_residues
MKFELDTTDGRARRGRLIFDRGTVETPAFMPVGTYGTVKGMTPEEVRATGADILLGNTFHLWLRPGEEIMRKHGDLHDFM
NWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKIFLDPEKSMQIQDALGSDVVMIFDECTPYPATEDEARKSMQ
MSLRWARRSRDEFDRLENPNSLFGIIQGGVYEDLRDESLKGLVDIGFDGYAVGGLAVGEPKADMHRILEHICPQIPADKP
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDTKCDCYTCKNYSRAYLYHLDR
CNEILGARLNTIHNLRYYQMLMEGLRGAIETGTLDAFVADFYTSQGREVPELVD
>Mature_374_residues
MKFELDTTDGRARRGRLIFDRGTVETPAFMPVGTYGTVKGMTPEEVRATGADILLGNTFHLWLRPGEEIMRKHGDLHDFM
NWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKIFLDPEKSMQIQDALGSDVVMIFDECTPYPATEDEARKSMQ
MSLRWARRSRDEFDRLENPNSLFGIIQGGVYEDLRDESLKGLVDIGFDGYAVGGLAVGEPKADMHRILEHICPQIPADKP
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDTKCDCYTCKNYSRAYLYHLDR
CNEILGARLNTIHNLRYYQMLMEGLRGAIETGTLDAFVADFYTSQGREVPELVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=361, Percent_Identity=44.3213296398892, Blast_Score=309, Evalue=3e-84,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=83.3333333333333, Blast_Score=660, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=42.2651933701657, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=168, Percent_Identity=30.3571428571429, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920510, Length=369, Percent_Identity=42.2764227642276, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI21355311, Length=252, Percent_Identity=25.7936507936508, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42414; Mature: 42414

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFELDTTDGRARRGRLIFDRGTVETPAFMPVGTYGTVKGMTPEEVRATGADILLGNTFH
CEEECCCCCCCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGEEIMRKHGDLHDFMNWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKI
EEECCCHHHHHHCCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCEE
FLDPEKSMQIQDALGSDVVMIFDECTPYPATEDEARKSMQMSLRWARRSRDEFDRLENPN
EECCCCCCHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
SLFGIIQGGVYEDLRDESLKGLVDIGFDGYAVGGLAVGEPKADMHRILEHICPQIPADKP
CEEEEECCCHHHHHHHHHHCCEEEECCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDT
CEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCCC
KCDCYTCKNYSRAYLYHLDRCNEILGARLNTIHNLRYYQMLMEGLRGAIETGTLDAFVAD
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FYTSQGREVPELVD
HHHHCCCCCHHHCC
>Mature Secondary Structure
MKFELDTTDGRARRGRLIFDRGTVETPAFMPVGTYGTVKGMTPEEVRATGADILLGNTFH
CEEECCCCCCCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGEEIMRKHGDLHDFMNWQRPILTDSGGFQVFSLGDIRKITEEGVHFRSPINGEKI
EEECCCHHHHHHCCCHHHHHCCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCEE
FLDPEKSMQIQDALGSDVVMIFDECTPYPATEDEARKSMQMSLRWARRSRDEFDRLENPN
EECCCCCCHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
SLFGIIQGGVYEDLRDESLKGLVDIGFDGYAVGGLAVGEPKADMHRILEHICPQIPADKP
CEEEEECCCHHHHHHHHHHCCEEEECCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTSEGVIKIRNARHRDDTSPLDT
CEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEECCCEEEECCCCCCCCCCCCCC
KCDCYTCKNYSRAYLYHLDRCNEILGARLNTIHNLRYYQMLMEGLRGAIETGTLDAFVAD
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FYTSQGREVPELVD
HHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA