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The map label for this gene is putP [H]

Identifier: 160875975

GI number: 160875975

Start: 3414284

End: 3415735

Strand: Direct

Name: putP [H]

Synonym: Sbal195_2865

Alternate gene names: 160875975

Gene position: 3414284-3415735 (Clockwise)

Preceding gene: 160875974

Following gene: 160875976

Centisome position: 63.85

GC content: 47.8

Gene sequence:

>1452_bases
ATGACGATTGAAACCCCGATTTTAATCACATTTGTTGGTTACTTAGTATTGATGATGGGCATAGGTTTTTGGGCTTACCG
TGCTACCGATACTGTTGATGATTATATTTTAGGTGGCCGCAAAATGGGCCCTGCGGTAACCGCACTCAGTGTGGGTGCAT
CCGATATGTCAGGTTGGCTGTTACTGGGTTTACCCGGCGCAGTTTACTTAGGCGGCTTAGGTGAAGCTTGGATTGGCATA
GGATTAATTTTTGGCGCTTGGCTAAACTGGCTATTTGTTGCCAGACGACTGCGTATTTACACTCAACTCGCCGATAACGC
CCTCACCTTACCGGATTTCTTCGAGAAACGTTTCCACGATACCCAGGGCTATCTCAAGCTCGTCTCCGCAATCACTATTT
TAGTGTTTTTCACTTTCTATGCTTCCTCAGGCATGGTCGGTGGCGCGATTCTGTTTGAAAAAGTTTTTGGCCTCGATTAC
ACAGTGGCGCTGGTGATTGGCTCAGCCATCATAGTCGGTTACACCTTTATTGGTGGCTTCTTTGCTGTGTGTTGGACTGA
CTTCTTTCAAGGCTGTTTGATGCTTGTCGCCCTCTTAATCGTCCCCTTTGCCGTTTTCTCTCACCCTGAAAGCCACGCCG
GAATTGAAACTATCGATCCTGCGATGTTGGCCTTGGTCAGTGACAAAACCACAGTGATAGGCATGTTGTCTTTACTCGCA
TGGGGTCTTGGCTATTTTGGTCAGCCGCATATTTTGTCGCGCTTTATGGCGATAGGCAGTGCCGATGCCCTGCCCTTGTC
CCGCCGTATTGCCATGAGCTGGATGGTCTTATCTTTAATTGGCGCATTAGCCACAGGTATTGCCGGTTCTCTGTATTTCG
CCAATGCGCCACTGGCGAATTCAGAAACGGTATTTATTCATTTAGCCCAAGCCGCGTTTAACCCTTGGATTGGTGGTCTA
CTTATTGCGGCCATTTTGTCGGCCATCATGAGCACTATCGATTCACAGTTGCTGGTGTGCTCAAGTGTTATCACCGAAGA
TTTTTACCGTAAATGGTTACGCCCTCAAGCGGATGATCGCGAGTTGATGATGGTCGGTCGCATGGGCGTGCTGGCGATTG
CCGTGATCGCAGGCATCATTGCCCTTAATCCTGAAAGCAGTGTATTAAGCCTTGTGAGTTATGCTTGGGCAGGCTTTGGT
GCGGCCTTTGGTCCTGTGGTCTTATTATCACTATTTTGGAAGCAATACAGCCGTAATGGTGCCATCGCCACTATTATTGT
CGGCGCATTAACAGTCGTGATTTGGAAGCAACTGACTGGGGGTATTTTCGAGTTATACGAAATCCTCCCAGGATTTGTAT
TCGCCACATTCGCCGGTATTTTGGTGAGCAAATTGTCAGCACCGAGTGAAAATGTAACAGCAGAGTTCGAACAATTTAAG
TCTGCACTTTAG

Upstream 100 bases:

>100_bases
GTTAGATTTTAATCACACTTCTATCTGCTAATGGGCGATTGTTCAAACATCTGGTAGCATGCTTGCCACTTTTAATAATA
ACAAGATGGGAAGAGGCAAG

Downstream 100 bases:

>100_bases
TTTCGACACGCTTGTATAACTTAAAAAGCGCACACTTGTGCGCTTTTTACATTTTGTATCCTTTTAAATACTCAACACAC
AATTACAAACCGTTAACGTC

Product: sodium/proline symporter

Products: NA

Alternate protein names: Proline permease; Propionate transporter [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MTIETPILITFVGYLVLMMGIGFWAYRATDTVDDYILGGRKMGPAVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGI
GLIFGAWLNWLFVARRLRIYTQLADNALTLPDFFEKRFHDTQGYLKLVSAITILVFFTFYASSGMVGGAILFEKVFGLDY
TVALVIGSAIIVGYTFIGGFFAVCWTDFFQGCLMLVALLIVPFAVFSHPESHAGIETIDPAMLALVSDKTTVIGMLSLLA
WGLGYFGQPHILSRFMAIGSADALPLSRRIAMSWMVLSLIGALATGIAGSLYFANAPLANSETVFIHLAQAAFNPWIGGL
LIAAILSAIMSTIDSQLLVCSSVITEDFYRKWLRPQADDRELMMVGRMGVLAIAVIAGIIALNPESSVLSLVSYAWAGFG
AAFGPVVLLSLFWKQYSRNGAIATIIVGALTVVIWKQLTGGIFELYEILPGFVFATFAGILVSKLSAPSENVTAEFEQFK
SAL

Sequences:

>Translated_483_residues
MTIETPILITFVGYLVLMMGIGFWAYRATDTVDDYILGGRKMGPAVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGI
GLIFGAWLNWLFVARRLRIYTQLADNALTLPDFFEKRFHDTQGYLKLVSAITILVFFTFYASSGMVGGAILFEKVFGLDY
TVALVIGSAIIVGYTFIGGFFAVCWTDFFQGCLMLVALLIVPFAVFSHPESHAGIETIDPAMLALVSDKTTVIGMLSLLA
WGLGYFGQPHILSRFMAIGSADALPLSRRIAMSWMVLSLIGALATGIAGSLYFANAPLANSETVFIHLAQAAFNPWIGGL
LIAAILSAIMSTIDSQLLVCSSVITEDFYRKWLRPQADDRELMMVGRMGVLAIAVIAGIIALNPESSVLSLVSYAWAGFG
AAFGPVVLLSLFWKQYSRNGAIATIIVGALTVVIWKQLTGGIFELYEILPGFVFATFAGILVSKLSAPSENVTAEFEQFK
SAL
>Mature_482_residues
TIETPILITFVGYLVLMMGIGFWAYRATDTVDDYILGGRKMGPAVTALSVGASDMSGWLLLGLPGAVYLGGLGEAWIGIG
LIFGAWLNWLFVARRLRIYTQLADNALTLPDFFEKRFHDTQGYLKLVSAITILVFFTFYASSGMVGGAILFEKVFGLDYT
VALVIGSAIIVGYTFIGGFFAVCWTDFFQGCLMLVALLIVPFAVFSHPESHAGIETIDPAMLALVSDKTTVIGMLSLLAW
GLGYFGQPHILSRFMAIGSADALPLSRRIAMSWMVLSLIGALATGIAGSLYFANAPLANSETVFIHLAQAAFNPWIGGLL
IAAILSAIMSTIDSQLLVCSSVITEDFYRKWLRPQADDRELMMVGRMGVLAIAVIAGIIALNPESSVLSLVSYAWAGFGA
AFGPVVLLSLFWKQYSRNGAIATIIVGALTVVIWKQLTGGIFELYEILPGFVFATFAGILVSKLSAPSENVTAEFEQFKS
AL

Specific function: Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=484, Percent_Identity=46.900826446281, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI4507035, Length=376, Percent_Identity=24.7340425531915, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI11141885, Length=402, Percent_Identity=26.6169154228856, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI17941285, Length=461, Percent_Identity=24.9457700650759, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787251, Length=488, Percent_Identity=52.8688524590164, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI87082237, Length=461, Percent_Identity=27.114967462039, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790503, Length=468, Percent_Identity=25.2136752136752, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1790113, Length=413, Percent_Identity=23.728813559322, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539284, Length=413, Percent_Identity=24.2130750605327, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI115533094, Length=367, Percent_Identity=23.9782016348774, Blast_Score=66, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24645928, Length=381, Percent_Identity=28.3464566929134, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221459584, Length=374, Percent_Identity=25.1336898395722, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24648033, Length=400, Percent_Identity=23.75, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356865, Length=400, Percent_Identity=23.75, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24640370, Length=362, Percent_Identity=25.414364640884, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24651739, Length=466, Percent_Identity=22.3175965665236, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI221459586, Length=378, Percent_Identity=23.2804232804233, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI221459588, Length=378, Percent_Identity=23.2804232804233, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 52034; Mature: 51903

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCC
>Mature Secondary Structure 
TIETPILITFVGYLVLMMGIGFWAYRATDTVDDYILGGRKMGPAVTALSVGASDMSGWL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHCCCCCCCCEE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HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3302614; 8905232; 9278503; 1400239; 3325781; 3053687; 3053649; 1567896; 8119910 [H]