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The map label for this gene is fadI

Identifier: 160875966

GI number: 160875966

Start: 3403884

End: 3405194

Strand: Reverse

Name: fadI

Synonym: Sbal195_2856

Alternate gene names: 160875966

Gene position: 3405194-3403884 (Counterclockwise)

Preceding gene: 160875983

Following gene: 160875965

Centisome position: 63.68

GC content: 50.72

Gene sequence:

>1311_bases
ATGAGTGATAGACAACAGGTTACGAACGCCAAGGGTGAGCGTATCGCAATTGTAGCGGGTTTAAGAACGCCATTTGCAAA
GCAAGCTACGGCATTCCACGGTGTATCGGCGCTTGATATGGGCAAAATGGTGGTCAATGAATTATTGGCGCGCTCTGAAC
TTGACCCTAAACTGATTGAGCAGCTGGTCTATGGACAAGTGGTGCAAATGCCCGCTGCACCTAACATCGCCCGTGAAATC
GTACTCGGTACTGGCATGAATGTGTCGACCGATGCCTATAGTGTCACTCGTGCCTGTGCGACTAGCTTCCAATCTGCCGT
AAACGTGGCCGAATCTATCATGACAGGCAATATCGAAATCGGTATTGCGGGCGGCGCCGACTCTTCTTCTGTCTTGCCTA
TCGGCGTGTCGAAAAAATTAGCCCATGCGTTAGTCGATCTGAATAAAGCCCGTAGCTTTGGGCAAAAATTACAAATTTTC
CGTCGTTTAGGCATTAAAGATTTATTACCTGTACCGCCTGCGGTAGCCGAGTACTCAACGGGCTTGTCTATGGGACAAAC
CGCCGAGCAAATGGCAAAAACCTACAATATTAGCCGCGCCGATCAAGATGCTTTAGCGCACCGCTCGCACACCTTAGCGA
GTGAAACTTGGGCTTCGGGCCATCTTCGTGACGAAGTGATGGTTGCCCATGTGCCGCCGTATAAACAGTTTATCGATCGC
GATAACAATATCCGCGAAAACTCGGTACTGGAGTCATATGCTAAGTTGCGCCCTGCTTTCGATAAACAGCATGGTACTGT
GACGGCGGCCAACAGTACGCCACTGACTGACGGTGCCTCAGCGATTATCCTGATGAGCGAAGGTCGAGCGAAAGCCTTAG
GTTATCAACCAATTGGTTACATTAAGAGCTATGCCTTTAGCGCCATAGATGTGTGGCAAGACATGCTGATGGGGCCCTCT
TACGCGACGCCACTGGCGCTGAAACGTGCTGGAATGGAGCTTGAAGATCTTACCCTGATTGAAATGCACGAAGCTTTTGC
AGCGCAGACATTAGCGAACATGCAGATGTTTGCTTCGAAAAAATTTGCCGAAGAAAAACTCGGTCGTAACCGCGCGATTG
GTGAAATCGACATGAGCAAGTTTAACGTGCTCGGCGGCTCGCTTGCCTACGGCCATCCTTTTGCTGCAACGGGAACCCGT
TTGATCACGCAAGTGTGTCGTGAGCTTAAGCGCCGTGGTGGTGGAACTGGCCTAACAACGGCCTGTGCGGCTGGTGGTTT
GGGCGTGGCAATGATAGTGGAAGTGGAGTAA

Upstream 100 bases:

>100_bases
GTTATTAGGCATTTTACGCCGTAAAACAATGGGTCAAACACGGAAATAGCTAGCGCCGCCTGAAATCTCTCAGCGATTGC
GCACATAAAGAGGGTGACAA

Downstream 100 bases:

>100_bases
TCGGCATGGAAAAGACCTTTAATTTAACCCGCCGCGACGACGGCATTGCTATTTTGACTATGGATGTTCCTGGCGAAACC
ATGAACACCCTAAAAGCGCA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIEQLVYGQVVQMPAAPNIAREI
VLGTGMNVSTDAYSVTRACATSFQSAVNVAESIMTGNIEIGIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIF
RRLGIKDLLPVPPAVAEYSTGLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHVPPYKQFIDR
DNNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGYIKSYAFSAIDVWQDMLMGPS
YATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASKKFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTR
LITQVCRELKRRGGGTGLTTACAAGGLGVAMIVEVE

Sequences:

>Translated_436_residues
MSDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIEQLVYGQVVQMPAAPNIAREI
VLGTGMNVSTDAYSVTRACATSFQSAVNVAESIMTGNIEIGIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIF
RRLGIKDLLPVPPAVAEYSTGLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHVPPYKQFIDR
DNNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGYIKSYAFSAIDVWQDMLMGPS
YATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASKKFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTR
LITQVCRELKRRGGGTGLTTACAAGGLGVAMIVEVE
>Mature_435_residues
SDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIEQLVYGQVVQMPAAPNIAREIV
LGTGMNVSTDAYSVTRACATSFQSAVNVAESIMTGNIEIGIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIFR
RLGIKDLLPVPPAVAEYSTGLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHVPPYKQFIDRD
NNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGYIKSYAFSAIDVWQDMLMGPSY
ATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASKKFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTRL
ITQVCRELKRRGGGTGLTTACAAGGLGVAMIVEVE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI4504327, Length=420, Percent_Identity=41.1904761904762, Blast_Score=333, Evalue=1e-91,
Organism=Homo sapiens, GI167614485, Length=423, Percent_Identity=32.1513002364066, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI4557237, Length=434, Percent_Identity=27.8801843317972, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI148539872, Length=438, Percent_Identity=29.6803652968037, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=28.8372093023256, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=72.7699530516432, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI87082165, Length=427, Percent_Identity=32.3185011709602, Blast_Score=199, Evalue=4e-52,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=34.0425531914894, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI48994986, Length=433, Percent_Identity=33.0254041570439, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1787663, Length=424, Percent_Identity=31.1320754716981, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=40.855106888361, Blast_Score=306, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=30.9859154929577, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI25147385, Length=420, Percent_Identity=28.0952380952381, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17535921, Length=426, Percent_Identity=27.2300469483568, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17535917, Length=430, Percent_Identity=23.0232558139535, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6325229, Length=430, Percent_Identity=28.6046511627907, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6322031, Length=432, Percent_Identity=29.8611111111111, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17137578, Length=434, Percent_Identity=42.1658986175115, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24655093, Length=424, Percent_Identity=29.2452830188679, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI17648125, Length=429, Percent_Identity=28.9044289044289, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24640423, Length=430, Percent_Identity=24.1860465116279, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FADI_SHEB2 (B8EE97)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002357525.1
- ProteinModelPortal:   B8EE97
- SMR:   B8EE97
- GeneID:   7089998
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_1597
- HOGENOM:   HBG370930
- OMA:   RDEVMTA
- ProtClustDB:   PRK08963
- GO:   GO:0005737
- HAMAP:   MF_01618
- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930
- TIGRFAMs:   TIGR02446

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: =2.3.1.16

Molecular weight: Translated: 46750; Mature: 46619

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 99-99 ACT_SITE 392-392 ACT_SITE 422-422

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIE
CCCHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QLVYGQVVQMPAAPNIAREIVLGTGMNVSTDAYSVTRACATSFQSAVNVAESIMTGNIEI
HHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIFRRLGIKDLLPVPPAVAEYST
EEECCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHC
GLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHVPPYKQFIDR
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCC
DNNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGY
CCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCHHCCCCCHHH
IKSYAFSAIDVWQDMLMGPSYATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTRLITQVCRELKRRGGGTGLTT
HHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGVAMIVEVE
HHHCCCCCEEEEEEEC
>Mature Secondary Structure 
SDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIE
CCHHHHHCCCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QLVYGQVVQMPAAPNIAREIVLGTGMNVSTDAYSVTRACATSFQSAVNVAESIMTGNIEI
HHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIFRRLGIKDLLPVPPAVAEYST
EEECCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHC
GLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHVPPYKQFIDR
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCC
DNNIRENSVLESYAKLRPAFDKQHGTVTAANSTPLTDGASAIILMSEGRAKALGYQPIGY
CCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEEEEEECCCCHHCCCCCHHH
IKSYAFSAIDVWQDMLMGPSYATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASK
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTRLITQVCRELKRRGGGTGLTT
HHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGVAMIVEVE
HHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA