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The map label for this gene is mltB [H]

Identifier: 160875817

GI number: 160875817

Start: 3213100

End: 3214416

Strand: Reverse

Name: mltB [H]

Synonym: Sbal195_2705

Alternate gene names: 160875817

Gene position: 3214416-3213100 (Counterclockwise)

Preceding gene: 160875820

Following gene: 160875816

Centisome position: 60.11

GC content: 49.73

Gene sequence:

>1317_bases
TTGATTAAAATTAAAGGGTTAACTTATCTGTTTGCGAGTATTGCCGCCTGTGTCAGTCTGAGTGCCTGTTCTAGCGTGCC
AGCGGCGAGTGCCAGTACTAAGGCAAGCACTAACGCGAGCAGTAGCAACGCAAGTGGCATAGTCGCTGAAGCCAACCCAA
TGCCCGATATCAGTGCTAGCGTACAAGCGAATGCCGAGGCATTTCCGAGTTGTGTGACTGGGCTACAAGCGCGGGCGCGC
ACTGAAGGGCTGTCAGAGGCGACGATTAACTCGACCATTGCAAATTTGAAATTTGTGCCTCGGGTGATTGAGCTCGATCA
GCAACAACCTGAATTCAGCCAGACTTTTGATAATTATTTCAGTAAGCGCGCCACCGAATGGCGCGTCAATGAAGGCCGTC
GCTTACTGCAAAAGCACAGCGTATTACTCAATCAATTAGCCAAAGAATATGGCGTGCCGCCGCAATATATCTTGTCTTTT
TGGGGGCTTGAAACGAACTATGGTTCCTATAAAGGCAAGATGTCGGTATTGAATTCGCTTGCGACTTTGGCCTGTGAGCC
ACGCCGTAGCAATTATTTTACTATAGAATTGATGCAAGCCTTAAAGCTGAAAGAAAAGTATCAGTTTGATGATGACACTA
TGGTGGGATCATGGGCTGGTGCTATGGGACACACGCAATTTATGCCGTCGGCCTATGCCAAATATGCCGTCGACGGTGAC
GGCGATGGTAAAGCCGATTTATGGAATAGCACCACAGACGCGCTGACTTCCGCCGCGAACTTTTTACAGCACTTAGGCTG
GCAGCGAAATGAACGCTGGGGCCGTGAAGTCTTATTACCAAAGGATTTTAGTTACAGCCATTTAGGGGCGAAACAGCCCT
TGGCGTTATCAGAGTGGGCCGCCCTTAATGTCACGCAAGCGAATGGTCAACCTTTGCCCGCCGTGGATATTCAAGCCGCG
TTGTATTTGCCAGCAGGCCATACAGGGCCTGCCTTTTTAGGCTATGAGAACTTCAATGTGATCATGCGTTGGAATCGCTC
CGAGTATTATGCCATTACAGTAGGGCATCTCGCCGATCGCATTAATGGTGGCTTACCTTTGACAGTGAGCCCGCCAGAAC
AGCCACGCTTAAGTCGCGAGCAAGTTAAGCAACTGCAAGCAAAACTGAATGAGGCCGGATTTGATGTTGGCAAGCCAGAT
GGTGTGTTGGGGCGTAACTCTGTGGCAGGGCTGCAAGCCTTTCAACGAAGCAAACAGATGATTGCCGATGGCTTCCCAAG
CCAAGAGGTGTTTAGCGCTTTAGGACTATCACTGTAG

Upstream 100 bases:

>100_bases
CTAGGGCAATGCCTTCTCGAAGAAGGAAGATTGTCGTGTTTAGCCACAGCAGTGTGATTACTGTGTCATTGTTGTGAAAT
CAAATATTGGAGTTGTTGAT

Downstream 100 bases:

>100_bases
CCACTGCCACTGTAGTTACTTTAGTCACTATAGCCAAAGAGCGCTCGCTGCGTGTTTTGAGCGCTTTTAGGCCGGATAAA
AAAGTGGAGTGAAAAGCTCA

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MIKIKGLTYLFASIAACVSLSACSSVPAASASTKASTNASSSNASGIVAEANPMPDISASVQANAEAFPSCVTGLQARAR
TEGLSEATINSTIANLKFVPRVIELDQQQPEFSQTFDNYFSKRATEWRVNEGRRLLQKHSVLLNQLAKEYGVPPQYILSF
WGLETNYGSYKGKMSVLNSLATLACEPRRSNYFTIELMQALKLKEKYQFDDDTMVGSWAGAMGHTQFMPSAYAKYAVDGD
GDGKADLWNSTTDALTSAANFLQHLGWQRNERWGREVLLPKDFSYSHLGAKQPLALSEWAALNVTQANGQPLPAVDIQAA
LYLPAGHTGPAFLGYENFNVIMRWNRSEYYAITVGHLADRINGGLPLTVSPPEQPRLSREQVKQLQAKLNEAGFDVGKPD
GVLGRNSVAGLQAFQRSKQMIADGFPSQEVFSALGLSL

Sequences:

>Translated_438_residues
MIKIKGLTYLFASIAACVSLSACSSVPAASASTKASTNASSSNASGIVAEANPMPDISASVQANAEAFPSCVTGLQARAR
TEGLSEATINSTIANLKFVPRVIELDQQQPEFSQTFDNYFSKRATEWRVNEGRRLLQKHSVLLNQLAKEYGVPPQYILSF
WGLETNYGSYKGKMSVLNSLATLACEPRRSNYFTIELMQALKLKEKYQFDDDTMVGSWAGAMGHTQFMPSAYAKYAVDGD
GDGKADLWNSTTDALTSAANFLQHLGWQRNERWGREVLLPKDFSYSHLGAKQPLALSEWAALNVTQANGQPLPAVDIQAA
LYLPAGHTGPAFLGYENFNVIMRWNRSEYYAITVGHLADRINGGLPLTVSPPEQPRLSREQVKQLQAKLNEAGFDVGKPD
GVLGRNSVAGLQAFQRSKQMIADGFPSQEVFSALGLSL
>Mature_438_residues
MIKIKGLTYLFASIAACVSLSACSSVPAASASTKASTNASSSNASGIVAEANPMPDISASVQANAEAFPSCVTGLQARAR
TEGLSEATINSTIANLKFVPRVIELDQQQPEFSQTFDNYFSKRATEWRVNEGRRLLQKHSVLLNQLAKEYGVPPQYILSF
WGLETNYGSYKGKMSVLNSLATLACEPRRSNYFTIELMQALKLKEKYQFDDDTMVGSWAGAMGHTQFMPSAYAKYAVDGD
GDGKADLWNSTTDALTSAANFLQHLGWQRNERWGREVLLPKDFSYSHLGAKQPLALSEWAALNVTQANGQPLPAVDIQAA
LYLPAGHTGPAFLGYENFNVIMRWNRSEYYAITVGHLADRINGGLPLTVSPPEQPRLSREQVKQLQAKLNEAGFDVGKPD
GVLGRNSVAGLQAFQRSKQMIADGFPSQEVFSALGLSL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=359, Percent_Identity=30.3621169916435, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 47738; Mature: 47738

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIKGLTYLFASIAACVSLSACSSVPAASASTKASTNASSSNASGIVAEANPMPDISAS
CEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
VQANAEAFPSCVTGLQARARTEGLSEATINSTIANLKFVPRVIELDQQQPEFSQTFDNYF
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SKRATEWRVNEGRRLLQKHSVLLNQLAKEYGVPPQYILSFWGLETNYGSYKGKMSVLNSL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHH
ATLACEPRRSNYFTIELMQALKLKEKYQFDDDTMVGSWAGAMGHTQFMPSAYAKYAVDGD
HHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCCEEHHHHCCCCCHHHCCHHHHHEEECCC
GDGKADLWNSTTDALTSAANFLQHLGWQRNERWGREVLLPKDFSYSHLGAKQPLALSEWA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHCCCCCCCCHHCCC
ALNVTQANGQPLPAVDIQAALYLPAGHTGPAFLGYENFNVIMRWNRSEYYAITVGHLADR
EEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCEEEEEECCCCEEEEEHHHHHHH
INGGLPLTVSPPEQPRLSREQVKQLQAKLNEAGFDVGKPDGVLGRNSVAGLQAFQRSKQM
HCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IADGFPSQEVFSALGLSL
HHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIKIKGLTYLFASIAACVSLSACSSVPAASASTKASTNASSSNASGIVAEANPMPDISAS
CEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
VQANAEAFPSCVTGLQARARTEGLSEATINSTIANLKFVPRVIELDQQQPEFSQTFDNYF
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SKRATEWRVNEGRRLLQKHSVLLNQLAKEYGVPPQYILSFWGLETNYGSYKGKMSVLNSL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHH
ATLACEPRRSNYFTIELMQALKLKEKYQFDDDTMVGSWAGAMGHTQFMPSAYAKYAVDGD
HHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCCEEHHHHCCCCCHHHCCHHHHHEEECCC
GDGKADLWNSTTDALTSAANFLQHLGWQRNERWGREVLLPKDFSYSHLGAKQPLALSEWA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHCCCCCCCCHHCCC
ALNVTQANGQPLPAVDIQAALYLPAGHTGPAFLGYENFNVIMRWNRSEYYAITVGHLADR
EEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCEEEEEECCCCEEEEEHHHHHHH
INGGLPLTVSPPEQPRLSREQVKQLQAKLNEAGFDVGKPDGVLGRNSVAGLQAFQRSKQM
HCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IADGFPSQEVFSALGLSL
HHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]