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The map label for this gene is rimK1

Identifier: 160875608

GI number: 160875608

Start: 2948178

End: 2949083

Strand: Reverse

Name: rimK1

Synonym: Sbal195_2496

Alternate gene names: 160875608

Gene position: 2949083-2948178 (Counterclockwise)

Preceding gene: 160875609

Following gene: 160875607

Centisome position: 55.15

GC content: 48.79

Gene sequence:

>906_bases
ATGAGAATAGCGATCTTATCGCAAGGGCCTGAGTTATATTCAACGAAGCGACTCGTTGAAGCGGCTCAATTACGTGGTCA
TGAAGTACACGTAATCAATCCCCTTGAATGTTATATGAATATCAACATGCGGCAATCGAGTATCCACATTGGTGGGCGAG
AGTTACCCGCATTTGATGCTGTGATCCCCCGTATTGGGGCGTCGATTACCTTTTATGGCTCGGCGGTGTTGCGCCAATTT
GAGATGATGGGCGTCTATGCTTTAAATGATTCTGTAGGGATCTCGCGCTCACGTGACAAATTGCGTTCTATGCAGTTGAT
GTCACGCCGCGGCATTGGCTTGCCCATTACGGGTTTTGCCAATAAACCGAGTGATATTCCCGACTTAATTGATATGGTGG
GTGGCGCGCCTCTGGTGATTAAATTACTCGAAGGCACCCAAGGTATTGGCGTGGTATTAGCCGAGACCCGTAAAGCGGCT
GAGAGCGTGATTGAAGCCTTTATGGGCCTTAAAGCCAACATTATGGTGCAGGAATATATCAAAGAGGCTAATGGTGCAGA
TATTCGCTGCTTTGTGCTCGGCGATAAAGTGATTGCCGCAATGAAGCGTCAAGCTATGCCCGGTGAGTTCCGCTCTAACT
TGCACCGTGGTGGCACGGCAAGTTTAGTCAAATTAACCCCAGAGGAACGCTCAGTCGCCATTCGTGCGGCTAAGACCATG
GGGCTCAATGTCGCTGGCGTTGACTTGCTACGCTCAAACCACGGTCCTGTGATCATGGAAGTGAACTCTTCACCAGGGCT
TGAAGGAATTGAAGGCGCCACCACGAAAGATGTGGCGGGTGCTATTATTGATTTTGTTGAAAAAAATGCGATTAAAGTGA
AAAAGGTGACGCAAGCACAGGGTTAG

Upstream 100 bases:

>100_bases
GAGCATTTGTGCAACTAATCTTGTCTATGCCTCTAGTTGTTTGATTTATCCCAGCGCAGGCGTATTATGCGCTCAAAATT
AATGGCTGCCGGAGACCAGA

Downstream 100 bases:

>100_bases
TGCCCTTGCTGTCGAGTATTGACTGCGGAATATCAACACTTGATTCACCTCCTTATGGGTAACGCCGTTTAGCAAGAACT
TACCCATTAAAGCGAACCGA

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRIAILSQGPELYSTKRLVEAAQLRGHEVHVINPLECYMNINMRQSSIHIGGRELPAFDAVIPRIGASITFYGSAVLRQF
EMMGVYALNDSVGISRSRDKLRSMQLMSRRGIGLPITGFANKPSDIPDLIDMVGGAPLVIKLLEGTQGIGVVLAETRKAA
ESVIEAFMGLKANIMVQEYIKEANGADIRCFVLGDKVIAAMKRQAMPGEFRSNLHRGGTASLVKLTPEERSVAIRAAKTM
GLNVAGVDLLRSNHGPVIMEVNSSPGLEGIEGATTKDVAGAIIDFVEKNAIKVKKVTQAQG

Sequences:

>Translated_301_residues
MRIAILSQGPELYSTKRLVEAAQLRGHEVHVINPLECYMNINMRQSSIHIGGRELPAFDAVIPRIGASITFYGSAVLRQF
EMMGVYALNDSVGISRSRDKLRSMQLMSRRGIGLPITGFANKPSDIPDLIDMVGGAPLVIKLLEGTQGIGVVLAETRKAA
ESVIEAFMGLKANIMVQEYIKEANGADIRCFVLGDKVIAAMKRQAMPGEFRSNLHRGGTASLVKLTPEERSVAIRAAKTM
GLNVAGVDLLRSNHGPVIMEVNSSPGLEGIEGATTKDVAGAIIDFVEKNAIKVKKVTQAQG
>Mature_301_residues
MRIAILSQGPELYSTKRLVEAAQLRGHEVHVINPLECYMNINMRQSSIHIGGRELPAFDAVIPRIGASITFYGSAVLRQF
EMMGVYALNDSVGISRSRDKLRSMQLMSRRGIGLPITGFANKPSDIPDLIDMVGGAPLVIKLLEGTQGIGVVLAETRKAA
ESVIEAFMGLKANIMVQEYIKEANGADIRCFVLGDKVIAAMKRQAMPGEFRSNLHRGGTASLVKLTPEERSVAIRAAKTM
GLNVAGVDLLRSNHGPVIMEVNSSPGLEGIEGATTKDVAGAIIDFVEKNAIKVKKVTQAQG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI164663810, Length=215, Percent_Identity=29.3023255813954, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI134254462, Length=214, Percent_Identity=29.4392523364486, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1787076, Length=290, Percent_Identity=67.5862068965517, Blast_Score=414, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMK1_SHEB8 (A6WNX9)

Other databases:

- EMBL:   CP000753
- RefSeq:   YP_001366581.1
- STRING:   A6WNX9
- GeneID:   5370816
- GenomeReviews:   CP000753_GR
- KEGG:   sbm:Shew185_2380
- eggNOG:   COG0189
- HOGENOM:   HBG679591
- OMA:   CREPRLY
- ProtClustDB:   CLSK906665
- BioCyc:   SBAL402882:SHEW185_2380-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_01552
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 32423; Mature: 32423

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAILSQGPELYSTKRLVEAAQLRGHEVHVINPLECYMNINMRQSSIHIGGRELPAFDA
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHEEEECCEEECEEEECCCCCCHHHH
VIPRIGASITFYGSAVLRQFEMMGVYALNDSVGISRSRDKLRSMQLMSRRGIGLPITGFA
HHHHHCCEEEEHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEECCCC
NKPSDIPDLIDMVGGAPLVIKLLEGTQGIGVVLAETRKAAESVIEAFMGLKANIMVQEYI
CCCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHH
KEANGADIRCFVLGDKVIAAMKRQAMPGEFRSNLHRGGTASLVKLTPEERSVAIRAAKTM
HHCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCHHHEEEEEHHHH
GLNVAGVDLLRSNHGPVIMEVNSSPGLEGIEGATTKDVAGAIIDFVEKNAIKVKKVTQAQ
CCCEEHHHHEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHCCC
G
C
>Mature Secondary Structure
MRIAILSQGPELYSTKRLVEAAQLRGHEVHVINPLECYMNINMRQSSIHIGGRELPAFDA
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHEEEECCEEECEEEECCCCCCHHHH
VIPRIGASITFYGSAVLRQFEMMGVYALNDSVGISRSRDKLRSMQLMSRRGIGLPITGFA
HHHHHCCEEEEHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEECCCC
NKPSDIPDLIDMVGGAPLVIKLLEGTQGIGVVLAETRKAAESVIEAFMGLKANIMVQEYI
CCCCCCHHHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHH
KEANGADIRCFVLGDKVIAAMKRQAMPGEFRSNLHRGGTASLVKLTPEERSVAIRAAKTM
HHCCCCEEEEEEECHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCHHHEEEEEHHHH
GLNVAGVDLLRSNHGPVIMEVNSSPGLEGIEGATTKDVAGAIIDFVEKNAIKVKKVTQAQ
CCCEEHHHHEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA