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The map label for this gene is aspS [H]

Identifier: 160875535

GI number: 160875535

Start: 2862680

End: 2864458

Strand: Reverse

Name: aspS [H]

Synonym: Sbal195_2423

Alternate gene names: 160875535

Gene position: 2864458-2862680 (Counterclockwise)

Preceding gene: 160875536

Following gene: 160875534

Centisome position: 53.57

GC content: 48.74

Gene sequence:

>1779_bases
ATGCGCAGTCATTATTGTGGAGACGTCAATAAGTCTCACGTTGGACAAGAAGTTACCTTGGTAGGTTGGGTTAATCGTAG
CCGTGATTTGGGTGGCGTTGTCTTTTTAGATTTAAGAGACAGAGAAGGTCTGATCCAAGTGGTCTATGATCCAGACTTAC
CTGAGGTGTTCAATGTCGCCAGCACGCTGCGCGCCGAATTTTGCGTTCAGGTTAAAGGCCTAGTTCGCGCCCGTCCTGAC
AGCCAAGTCAATGGTCAAATGAAGACGGGTGAGATTGAAGTCTTAGGCCAAGCCTTGACTATCATCAACGCTGCCGATCC
ACTGCCATTGAGCATGGATAACTACCAGAATAACAGTGAAGAGCAACGTCTTAAGTATCGTTACTTAGATTTACGTCGTC
CAGAAATGGCGCAGCGATTAATTTTCCGCGCTAAAGTGACCAGTTCTGTGCGTCGTTTCCTCGACTCAAACGGTTTCTTA
GACATAGAAACACCGATCCTGACCAAGGCAACCCCAGAAGGTGCCCGCGATTATTTAGTACCAAGCCGGACTTATAAAGG
TCAGTTCTTTGCCTTGCCACAATCGCCACAGCTGTTTAAACAGCTGCTGATGATGTCAGGCTTTGATCGTTACTACCAAA
TCGTAAAATGCTTCCGTGATGAAGACTTACGTGCCGACCGTCAGCCAGAATTTACCCAAATCGATATCGAAACGTCTTTC
ATGAGCTCTGAGCAAGTGATGGCGAAAACCGAAGAGATGATGCGTGGTCTGTTCCTTGAAATGCTGAACGTCGATCTCGG
CGAATTCCCACGCATGACCTACAACGAAGCGATGCGTCGTTTCGGTTCAGATAAGCCAGATTTACGTAACCCATTAGAGT
TGGTGGACATTGCCGATTTACTAAAAGAAGTCGAATTTGCGGTATTCTCAGGCCCTGCTAACGATGAAGAAGGCCGTGTT
GCTGCACTGCGTATTCCAGGCGGCGCTGCACTGTCTCGTAAGCAAATAGATGATTATACGAAGTTTGTTGGCATCTATGG
TGCTAAAGGCTTAGCGTGGATGAAGATTAATGACCTAAGCCTTGGCTTAGAGGGCATTCAATCACCTGTGCTGAAATTCT
TAAACGAAAGCATAGTCAATGAAATCGTGAGCCGCACGGCAGCGCAAACCGGTGACATTATTCTGTTTGGTGCTGACCAA
GCGACAGTGGTTGCCGAATCTATGGGCGCACTGCGTCTTAAAGCGGGCGAAGATTTTAACTTACTGCAAGGCGAATGGCG
TCCACTGTGGGTGGTTGACTTCCCAATGTTTGAGAAAATCAACGGCAACTTCCACGCTGTTCACCATCCGTTCACCGCAC
CGCGTGGCGTAACCGCAGCAGAACTTGAAGCTAACCCAGCTAATCGCGTTTCTGATGCCTACGATATGGTATTAAACGGC
TGCGAATTAGGCGGTGGTTCAGTGCGTATCCACAACCAAGAAATGCAGTCTGCGGTATTCCGTATTCTGGGTATTACCGA
CGAAGAAGCCAAAGAGAAGTTCGGCTTCCTGTTAGAAGCGCTGCGTTACGGTACTCCGCCACATGCAGGTTTAGCGTTTG
GTTTAGACCGCATCATCATGCTGATGACGGGCGCAAGCTCAATCCGCGATGTGATGGCGTTCCCGAAAACCACTACAGCC
GCTTGCCCGCTGACTAACGCACCAGGATTTGCAAATCCGCAGCAGTTAGCTGAACTTGGCATTGCCGTGGTCGAGAAAGC
CGTTAAAACGGAAGACTGA

Upstream 100 bases:

>100_bases
TGAAGTACTTTGGTAATCGGCATTATTTTACTATAATGCCGCTTTTATCTCGTTTTGATTATTTCCAAAGCACCGGTTTC
CGGATAAAGGATAGAGTTAG

Downstream 100 bases:

>100_bases
TCTTTCGTGAAAGGATTTTTGTAAATCTATCTTATCTAAGTCGATTGGCAATAATCCTTGTCAGTTACTGTTTAAATAGT
ACCGAAAACCGAGGCGCAAG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLIQVVYDPDLPEVFNVASTLRAEFCVQVKGLVRARPD
SQVNGQMKTGEIEVLGQALTIINAADPLPLSMDNYQNNSEEQRLKYRYLDLRRPEMAQRLIFRAKVTSSVRRFLDSNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MSSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDIADLLKEVEFAVFSGPANDEEGRV
AALRIPGGAALSRKQIDDYTKFVGIYGAKGLAWMKINDLSLGLEGIQSPVLKFLNESIVNEIVSRTAAQTGDIILFGADQ
ATVVAESMGALRLKAGEDFNLLQGEWRPLWVVDFPMFEKINGNFHAVHHPFTAPRGVTAAELEANPANRVSDAYDMVLNG
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIMLMTGASSIRDVMAFPKTTTA
ACPLTNAPGFANPQQLAELGIAVVEKAVKTED

Sequences:

>Translated_592_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLIQVVYDPDLPEVFNVASTLRAEFCVQVKGLVRARPD
SQVNGQMKTGEIEVLGQALTIINAADPLPLSMDNYQNNSEEQRLKYRYLDLRRPEMAQRLIFRAKVTSSVRRFLDSNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MSSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDIADLLKEVEFAVFSGPANDEEGRV
AALRIPGGAALSRKQIDDYTKFVGIYGAKGLAWMKINDLSLGLEGIQSPVLKFLNESIVNEIVSRTAAQTGDIILFGADQ
ATVVAESMGALRLKAGEDFNLLQGEWRPLWVVDFPMFEKINGNFHAVHHPFTAPRGVTAAELEANPANRVSDAYDMVLNG
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIMLMTGASSIRDVMAFPKTTTA
ACPLTNAPGFANPQQLAELGIAVVEKAVKTED
>Mature_592_residues
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLIQVVYDPDLPEVFNVASTLRAEFCVQVKGLVRARPD
SQVNGQMKTGEIEVLGQALTIINAADPLPLSMDNYQNNSEEQRLKYRYLDLRRPEMAQRLIFRAKVTSSVRRFLDSNGFL
DIETPILTKATPEGARDYLVPSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MSSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDIADLLKEVEFAVFSGPANDEEGRV
AALRIPGGAALSRKQIDDYTKFVGIYGAKGLAWMKINDLSLGLEGIQSPVLKFLNESIVNEIVSRTAAQTGDIILFGADQ
ATVVAESMGALRLKAGEDFNLLQGEWRPLWVVDFPMFEKINGNFHAVHHPFTAPRGVTAAELEANPANRVSDAYDMVLNG
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIMLMTGASSIRDVMAFPKTTTA
ACPLTNAPGFANPQQLAELGIAVVEKAVKTED

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=39.3989983305509, Blast_Score=408, Evalue=1e-113,
Organism=Homo sapiens, GI5031815, Length=249, Percent_Identity=28.1124497991968, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI194272210, Length=249, Percent_Identity=28.1124497991968, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI45439306, Length=265, Percent_Identity=27.9245283018868, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI226491502, Length=262, Percent_Identity=27.4809160305344, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4758762, Length=283, Percent_Identity=25.4416961130742, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788173, Length=589, Percent_Identity=68.7606112054329, Blast_Score=850, Evalue=0.0,
Organism=Escherichia coli, GI1790571, Length=293, Percent_Identity=27.9863481228669, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789256, Length=293, Percent_Identity=27.6450511945392, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=612, Percent_Identity=33.4967320261438, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI71994340, Length=179, Percent_Identity=27.9329608938547, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=179, Percent_Identity=27.9329608938547, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=179, Percent_Identity=27.9329608938547, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=306, Percent_Identity=26.1437908496732, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=275, Percent_Identity=26.1818181818182, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=323, Percent_Identity=37.4613003095975, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321807, Length=249, Percent_Identity=27.710843373494, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323011, Length=268, Percent_Identity=27.9850746268657, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324256, Length=178, Percent_Identity=25.8426966292135, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=262, Percent_Identity=24.8091603053435, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24584738, Length=608, Percent_Identity=35.1973684210526, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24640849, Length=246, Percent_Identity=27.2357723577236, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24640851, Length=246, Percent_Identity=27.2357723577236, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17136276, Length=248, Percent_Identity=28.6290322580645, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66003; Mature: 66003

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLIQVVYDPDLPEVFNVA
CCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHH
STLRAEFCVQVKGLVRARPDSQVNGQMKTGEIEVLGQALTIINAADPLPLSMDNYQNNSE
HHHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHEEECCCCCCCCCCCCCCCCH
EQRLKYRYLDLRRPEMAQRLIFRAKVTSSVRRFLDSNGFLDIETPILTKATPEGARDYLV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCC
PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
CCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHH
MSSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDIADL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
LKEVEFAVFSGPANDEEGRVAALRIPGGAALSRKQIDDYTKFVGIYGAKGLAWMKINDLS
HHHHHHHEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHH
LGLEGIQSPVLKFLNESIVNEIVSRTAAQTGDIILFGADQATVVAESMGALRLKAGEDFN
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCEEEECCCCCC
LLQGEWRPLWVVDFPMFEKINGNFHAVHHPFTAPRGVTAAELEANPANRVSDAYDMVLNG
CCCCCCCEEEEECCCHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHC
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIM
CCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LMTGASSIRDVMAFPKTTTAACPLTNAPGFANPQQLAELGIAVVEKAVKTED
HHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLIQVVYDPDLPEVFNVA
CCCCCCCCCCHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHH
STLRAEFCVQVKGLVRARPDSQVNGQMKTGEIEVLGQALTIINAADPLPLSMDNYQNNSE
HHHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHHHHHHHHEEECCCCCCCCCCCCCCCCH
EQRLKYRYLDLRRPEMAQRLIFRAKVTSSVRRFLDSNGFLDIETPILTKATPEGARDYLV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCC
PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
CCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHH
MSSEQVMAKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDIADL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHH
LKEVEFAVFSGPANDEEGRVAALRIPGGAALSRKQIDDYTKFVGIYGAKGLAWMKINDLS
HHHHHHHEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHH
LGLEGIQSPVLKFLNESIVNEIVSRTAAQTGDIILFGADQATVVAESMGALRLKAGEDFN
HCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCCEEEECCCCCC
LLQGEWRPLWVVDFPMFEKINGNFHAVHHPFTAPRGVTAAELEANPANRVSDAYDMVLNG
CCCCCCCEEEEECCCHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHC
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIM
CCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LMTGASSIRDVMAFPKTTTAACPLTNAPGFANPQQLAELGIAVVEKAVKTED
HHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA