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The map label for this gene is pspA [H]

Identifier: 160874757

GI number: 160874757

Start: 1982107

End: 1982790

Strand: Direct

Name: pspA [H]

Synonym: Sbal195_1640

Alternate gene names: 160874757

Gene position: 1982107-1982790 (Clockwise)

Preceding gene: 160874748

Following gene: 160874758

Centisome position: 37.07

GC content: 44.3

Gene sequence:

>684_bases
ATGGGAATTTTCTCTCGTTTCGCCGATATCATTAACTCTAATATCAGCGCATTGCTGGATAAAGCCGAAGACCCAGAAAA
AATGGTTCGACTGATTATCCAAGAAATGGAAGACACACTCGTTGAAGTGCGTTCTACCTCAGCTAAAGTGTTAGCTGAAA
AGAAAGAGCTGCAACGTCGTATCAACCGCGTGTTAGAGCAAGTGCAAGATTGGCAGGATAAGGCTGAACTGGCACTGTCC
AAAGACCGTGAAGACTTAGCGAAAGCCGCTTTAGTTGAAAAACAAAAAGCGACCAGTTTAGCAGACACGCTAAATCAAGA
GTTAGCCGTTATCGATGAACATATCGCACGCTTGAAAGATGAAGTGGGCATGTTACAAGAAAAGCTGCTCGATGCAAAAG
CGCGCCAAAAGACTATTATTTTGCGCACTCAAACAGCGTCATCACGCTTAGAAGTGAAAAAGCAGTTAGATTCAAGCAAG
ATCGACAACGCTATGCTCAAGTTTGAGCAATATGAGCGCCGCGTCGAAGGCTTAGAAGCCCAAGTTGAATCTTACGATCT
TGGCAGCAAAAAAACCTTAGCCGATGAGTTTGCGGCGCTAGAAGCGGAAGATTCTGTGAATGCAGAACTGGCAGCGCTAA
AAGCCAAGGTAAAAGGTAAAGCGCCAACTAAGTCAAAAGAATAA

Upstream 100 bases:

>100_bases
TAAAATTTTGTTTAAGCTGCTGTATTTAAAAAAGAAGTAAAAGTTGGCACGGTTAGTGTATTACCTTATACGAGACCAAC
ATCAATTTGAGGATAGGATT

Downstream 100 bases:

>100_bases
CAATTCAGAGGTGAGTTATGGATATGGACGTGCTAATGGCGCCGATTATTATTTTCATGGTGATAGTCGCGCCAATATGG
TTAGTTCTTCATTATCGCAG

Product: phage shock protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKELQRRINRVLEQVQDWQDKAELALS
KDREDLAKAALVEKQKATSLADTLNQELAVIDEHIARLKDEVGMLQEKLLDAKARQKTIILRTQTASSRLEVKKQLDSSK
IDNAMLKFEQYERRVEGLEAQVESYDLGSKKTLADEFAALEAEDSVNAELAALKAKVKGKAPTKSKE

Sequences:

>Translated_227_residues
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKELQRRINRVLEQVQDWQDKAELALS
KDREDLAKAALVEKQKATSLADTLNQELAVIDEHIARLKDEVGMLQEKLLDAKARQKTIILRTQTASSRLEVKKQLDSSK
IDNAMLKFEQYERRVEGLEAQVESYDLGSKKTLADEFAALEAEDSVNAELAALKAKVKGKAPTKSKE
>Mature_226_residues
GIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKELQRRINRVLEQVQDWQDKAELALSK
DREDLAKAALVEKQKATSLADTLNQELAVIDEHIARLKDEVGMLQEKLLDAKARQKTIILRTQTASSRLEVKKQLDSSKI
DNAMLKFEQYERRVEGLEAQVESYDLGSKKTLADEFAALEAEDSVNAELAALKAKVKGKAPTKSKE

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=218, Percent_Identity=57.7981651376147, Blast_Score=256, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25540; Mature: 25408

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKELQRR
CCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INRVLEQVQDWQDKAELALSKDREDLAKAALVEKQKATSLADTLNQELAVIDEHIARLKD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGMLQEKLLDAKARQKTIILRTQTASSRLEVKKQLDSSKIDNAMLKFEQYERRVEGLEA
HHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVESYDLGSKKTLADEFAALEAEDSVNAELAALKAKVKGKAPTKSKE
HHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKELQRR
CHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INRVLEQVQDWQDKAELALSKDREDLAKAALVEKQKATSLADTLNQELAVIDEHIARLKD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGMLQEKLLDAKARQKTIILRTQTASSRLEVKKQLDSSKIDNAMLKFEQYERRVEGLEA
HHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVESYDLGSKKTLADEFAALEAEDSVNAELAALKAKVKGKAPTKSKE
HHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]