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The map label for this gene is vat [H]

Identifier: 160874717

GI number: 160874717

Start: 1928148

End: 1928804

Strand: Reverse

Name: vat [H]

Synonym: Sbal195_1600

Alternate gene names: 160874717

Gene position: 1928804-1928148 (Counterclockwise)

Preceding gene: 160874718

Following gene: 160874716

Centisome position: 36.07

GC content: 47.95

Gene sequence:

>657_bases
ATGCCTGACACGACAATCCACGCCCAATTAGGCCCAAACCCTAACGACAAAGAACCGATGAAAGGCTTCCCTCAGGTTGG
ATTTCTTAAAAACTTTATTACCCGCGAGAACATTATCGTCGGCGATTATACCTATTATGACGATCCTGCTGGGCCTGAAA
GATTCGAGTCGAATGTGCTTTATCACTTTGACTTTATTGGCGATAAACTGATTATCGGTAAATTCTGCGCCATTGCTAAA
GACGTGAAATTCATTATGAATGGCGCCAATCATCAAGTGTCTGGCTTCTCGACTTATCCCTTTTATATCTTCGGCAATGG
CTGGGAAAAGGTAATGCCAGATCCCACCGATTTGCCCCATAAAGGCGACACCTGTATCGGTAACGATGTGTGGATTGGTT
ATAACGCCACTATCATGCCTGGAGTGAAGATTGGCCACGGCGCAATTGTTGCCAGTCAATCCGTGGTGACAAGAGATGTG
CCGCCCTACGCCGTGGTTGGCGGTAATCCTGCCACTGTCATCAAGCTCAGGTTCGAGCAAGATGTGATAGATAAGCTCAT
TGCCATCGCTTGGTGGGATTGGCCGATAGAGAAAATCACTCAGCATCTACACGCCATCGCGGGCGCGGATCTGACTCAAT
TACAGCAGGCGACCTAA

Upstream 100 bases:

>100_bases
CAGGCACCACAATCAACATCGCAATAGGCACCACAAATTCGCACATCAATCTCATAAAAGAATGCACGAGTTAAGACGAA
CTTTAACGATGGAGACCGTT

Downstream 100 bases:

>100_bases
GCAAAGCAGTCGAAAGGCAAGACTGAGCCCTCAAATATCATGTAACTAATATCTCGAGCGGCTAATATATCAAGAGACAA
ATATATCAACTGGCTAATAT

Product: hexapaptide repeat-containing transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPDTTIHAQLGPNPNDKEPMKGFPQVGFLKNFITRENIIVGDYTYYDDPAGPERFESNVLYHFDFIGDKLIIGKFCAIAK
DVKFIMNGANHQVSGFSTYPFYIFGNGWEKVMPDPTDLPHKGDTCIGNDVWIGYNATIMPGVKIGHGAIVASQSVVTRDV
PPYAVVGGNPATVIKLRFEQDVIDKLIAIAWWDWPIEKITQHLHAIAGADLTQLQQAT

Sequences:

>Translated_218_residues
MPDTTIHAQLGPNPNDKEPMKGFPQVGFLKNFITRENIIVGDYTYYDDPAGPERFESNVLYHFDFIGDKLIIGKFCAIAK
DVKFIMNGANHQVSGFSTYPFYIFGNGWEKVMPDPTDLPHKGDTCIGNDVWIGYNATIMPGVKIGHGAIVASQSVVTRDV
PPYAVVGGNPATVIKLRFEQDVIDKLIAIAWWDWPIEKITQHLHAIAGADLTQLQQAT
>Mature_217_residues
PDTTIHAQLGPNPNDKEPMKGFPQVGFLKNFITRENIIVGDYTYYDDPAGPERFESNVLYHFDFIGDKLIIGKFCAIAKD
VKFIMNGANHQVSGFSTYPFYIFGNGWEKVMPDPTDLPHKGDTCIGNDVWIGYNATIMPGVKIGHGAIVASQSVVTRDVP
PYAVVGGNPATVIKLRFEQDVIDKLIAIAWWDWPIEKITQHLHAIAGADLTQLQQAT

Specific function: Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=50, Percent_Identity=64, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 24192; Mature: 24061

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTTIHAQLGPNPNDKEPMKGFPQVGFLKNFITRENIIVGDYTYYDDPAGPERFESNVL
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCEECCCCCCHHHHHCCEE
YHFDFIGDKLIIGKFCAIAKDVKFIMNGANHQVSGFSTYPFYIFGNGWEKVMPDPTDLPH
EEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCCHHCCCCCCCCCC
KGDTCIGNDVWIGYNATIMPGVKIGHGAIVASQSVVTRDVPPYAVVGGNPATVIKLRFEQ
CCCEECCCCEEECCCCEECCCCEECCCEEEECCCEEECCCCCEEEECCCCEEEEEEECCH
DVIDKLIAIAWWDWPIEKITQHLHAIAGADLTQLQQAT
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure 
PDTTIHAQLGPNPNDKEPMKGFPQVGFLKNFITRENIIVGDYTYYDDPAGPERFESNVL
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCEECCCCCCHHHHHCCEE
YHFDFIGDKLIIGKFCAIAKDVKFIMNGANHQVSGFSTYPFYIFGNGWEKVMPDPTDLPH
EEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCCHHCCCCCCCCCC
KGDTCIGNDVWIGYNATIMPGVKIGHGAIVASQSVVTRDVPPYAVVGGNPATVIKLRFEQ
CCCEECCCCEEECCCCEECCCCEECCCEEEECCCEEECCCCCEEEECCCCEEEEEEECCH
DVIDKLIAIAWWDWPIEKITQHLHAIAGADLTQLQQAT
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 90 {maltose}} 62 {glucose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344533; 3149758 [H]