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The map label for this gene is ybjS [C]

Identifier: 160874700

GI number: 160874700

Start: 1907112

End: 1908311

Strand: Direct

Name: ybjS [C]

Synonym: Sbal195_1583

Alternate gene names: 160874700

Gene position: 1907112-1908311 (Clockwise)

Preceding gene: 160874699

Following gene: 160874701

Centisome position: 35.67

GC content: 49.67

Gene sequence:

>1200_bases
ATGACGATAAACCCAATCAAGAGCACGACGCTTGCGCAATTTGATGCGGCTGAACAAACTGCACTCGCGCAGCTCGCCCT
TAAGGTGCAACACGCCTTTGTGACGGGAGCGGGGGGATTTTTAGGTAAAGCGATTTGTTTGAGATTGTTAGCCGCGGGCA
TTAAGGTCACAGGTTTTGCCCGTGGGCATTATCCCGAACTTGAAGCGCTGGGCGTAGTCATGTTGCAAGGGGATTTAGTC
AATAAAGACCAGTTGCAACAGGCAATGCAAGGCTGCGATATCGTGTTTCATGTGGCGTCTAAAGCCGGCGTTTGGGGCGA
TAGAGACAGCTATTTTTGCCCGAATGTGAAGGGCGCAGCGAATGTGATTGCAGCTTGTAAAGCATTAAAAATAAATAAGT
TAGTCTACACCAGTACCCCGAGTGTAACCTTTGCGGGGCAGGATGAATCCGGCATAGATGAATCCACTCCCTATGCCACA
TCCTTCCTTAATTATTACGCCCATTCCAAAGCCATCGCCGAAAAGATGATGCTTGATGCCAATCAAGTGGGTGACGTATC
GGTTGAAAGTGCCGCTGCAACTCAAGCCACTCAAGCCACGACAGCCACTATTTCAGACAATAATCTAGCCACTACTCAAG
TCGCTATCCCGAACACATCTCAAGTCACTTCACCCTACGCGTTAAAAACTGTCGCGCTCAGGCCGCATTTGATCTGGGGT
CCTGGCGATCCCCATCTTGTACCTCGAGTACTGGCGCGTGGCCGTTTAGATAAGCTTAAACTGGTTGGCCGTGAAGATAA
GCTGGTCGATACCATCTACATAGACAACGCCGCCTATGCCCATGTGCTGGCCGCGCTAGAACTGTGCCAAGCAAAGCCTA
AGTGTCAGGGGAAAGCCTATTTTTTGAGTAACGATGAACCCATCACTATGGCCAAAATGCTCAATCTTATTTTGGCCTGC
GATGCTTTGCCGCCAGTCACTAACCGCGTGCCGCAATCTGTGGCCTATGTGGCAGGCGCCGTGTTGGAAATTGTGTATTT
TCTACTAAAGAAGCAAGAAGAGCCCATGATGACGCGCTTCGTGGCACGGCAGTTATCCTGCAGCCATTATTTTGATATCA
GCGCCGCCAAGCGAGATTTAGGCTATCGTGCACTTATCTCTATCAATGAAGGAATGGCAAGACTTAAAGCGTCACTCTAG

Upstream 100 bases:

>100_bases
TTCATTTGAACAAACTCGCTTATTTTTAAGCCCTTGTGCTTATTTTAAACTTCTTACACCGACTTTAAGTTCATGTAAAT
GTGCTGAAATGGATACCTAA

Downstream 100 bases:

>100_bases
TGTTATTAGGCGTGAGTTTCTGAGTGTGCTTGATATTCAATATGCCTGCTCTTATTTGCACTCTTATTGGCACTATTATT
GTGGGCATAGTTTAGCTGGG

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTINPIKSTTLAQFDAAEQTALAQLALKVQHAFVTGAGGFLGKAICLRLLAAGIKVTGFARGHYPELEALGVVMLQGDLV
NKDQLQQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKALKINKLVYTSTPSVTFAGQDESGIDESTPYAT
SFLNYYAHSKAIAEKMMLDANQVGDVSVESAAATQATQATTATISDNNLATTQVAIPNTSQVTSPYALKTVALRPHLIWG
PGDPHLVPRVLARGRLDKLKLVGREDKLVDTIYIDNAAYAHVLAALELCQAKPKCQGKAYFLSNDEPITMAKMLNLILAC
DALPPVTNRVPQSVAYVAGAVLEIVYFLLKKQEEPMMTRFVARQLSCSHYFDISAAKRDLGYRALISINEGMARLKASL

Sequences:

>Translated_399_residues
MTINPIKSTTLAQFDAAEQTALAQLALKVQHAFVTGAGGFLGKAICLRLLAAGIKVTGFARGHYPELEALGVVMLQGDLV
NKDQLQQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKALKINKLVYTSTPSVTFAGQDESGIDESTPYAT
SFLNYYAHSKAIAEKMMLDANQVGDVSVESAAATQATQATTATISDNNLATTQVAIPNTSQVTSPYALKTVALRPHLIWG
PGDPHLVPRVLARGRLDKLKLVGREDKLVDTIYIDNAAYAHVLAALELCQAKPKCQGKAYFLSNDEPITMAKMLNLILAC
DALPPVTNRVPQSVAYVAGAVLEIVYFLLKKQEEPMMTRFVARQLSCSHYFDISAAKRDLGYRALISINEGMARLKASL
>Mature_398_residues
TINPIKSTTLAQFDAAEQTALAQLALKVQHAFVTGAGGFLGKAICLRLLAAGIKVTGFARGHYPELEALGVVMLQGDLVN
KDQLQQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAANVIAACKALKINKLVYTSTPSVTFAGQDESGIDESTPYATS
FLNYYAHSKAIAEKMMLDANQVGDVSVESAAATQATQATTATISDNNLATTQVAIPNTSQVTSPYALKTVALRPHLIWGP
GDPHLVPRVLARGRLDKLKLVGREDKLVDTIYIDNAAYAHVLAALELCQAKPKCQGKAYFLSNDEPITMAKMLNLILACD
ALPPVTNRVPQSVAYVAGAVLEIVYFLLKKQEEPMMTRFVARQLSCSHYFDISAAKRDLGYRALISINEGMARLKASL

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=371, Percent_Identity=29.6495956873315, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI8393516, Length=371, Percent_Identity=29.6495956873315, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI116268111, Length=381, Percent_Identity=28.0839895013123, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI239745448, Length=366, Percent_Identity=28.1420765027322, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI310132178, Length=366, Percent_Identity=28.1420765027322, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI310113012, Length=366, Percent_Identity=28.1420765027322, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4504507, Length=386, Percent_Identity=29.7927461139896, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI260763931, Length=384, Percent_Identity=28.6458333333333, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4504509, Length=384, Percent_Identity=28.6458333333333, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321437, Length=387, Percent_Identity=29.4573643410853, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 42952; Mature: 42821

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTINPIKSTTLAQFDAAEQTALAQLALKVQHAFVTGAGGFLGKAICLRLLAAGIKVTGFA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCEEEEEE
RGHYPELEALGVVMLQGDLVNKDQLQQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAA
CCCCCCHHHHEEEEEECCCCCHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCCCCHHH
NVIAACKALKINKLVYTSTPSVTFAGQDESGIDESTPYATSFLNYYAHSKAIAEKMMLDA
HHHHHHHHHEECEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NQVGDVSVESAAATQATQATTATISDNNLATTQVAIPNTSQVTSPYALKTVALRPHLIWG
CCCCCCCCHHHHHHHHHHCEEEEECCCCEEEEEEECCCCCCCCCCHHEEEEEECCEEEEC
PGDPHLVPRVLARGRLDKLKLVGREDKLVDTIYIDNAAYAHVLAALELCQAKPKCQGKAY
CCCCHHHHHHHHCCCCCHHEECCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEE
FLSNDEPITMAKMLNLILACDALPPVTNRVPQSVAYVAGAVLEIVYFLLKKQEEPMMTRF
EECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VARQLSCSHYFDISAAKRDLGYRALISINEGMARLKASL
HHHHHCCCCEEECHHHHHCCCCEEEEEEHHHHHHHHHCC
>Mature Secondary Structure 
TINPIKSTTLAQFDAAEQTALAQLALKVQHAFVTGAGGFLGKAICLRLLAAGIKVTGFA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCEEEEEE
RGHYPELEALGVVMLQGDLVNKDQLQQAMQGCDIVFHVASKAGVWGDRDSYFCPNVKGAA
CCCCCCHHHHEEEEEECCCCCHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCCCCHHH
NVIAACKALKINKLVYTSTPSVTFAGQDESGIDESTPYATSFLNYYAHSKAIAEKMMLDA
HHHHHHHHHEECEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NQVGDVSVESAAATQATQATTATISDNNLATTQVAIPNTSQVTSPYALKTVALRPHLIWG
CCCCCCCCHHHHHHHHHHCEEEEECCCCEEEEEEECCCCCCCCCCHHEEEEEECCEEEEC
PGDPHLVPRVLARGRLDKLKLVGREDKLVDTIYIDNAAYAHVLAALELCQAKPKCQGKAY
CCCCHHHHHHHHCCCCCHHEECCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEE
FLSNDEPITMAKMLNLILACDALPPVTNRVPQSVAYVAGAVLEIVYFLLKKQEEPMMTRF
EECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VARQLSCSHYFDISAAKRDLGYRALISINEGMARLKASL
HHHHHCCCCEEECHHHHHCCCCEEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]