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The map label for this gene is kefB [H]

Identifier: 160874616

GI number: 160874616

Start: 1804900

End: 1806846

Strand: Direct

Name: kefB [H]

Synonym: Sbal195_1499

Alternate gene names: 160874616

Gene position: 1804900-1806846 (Clockwise)

Preceding gene: 160874614

Following gene: 160874618

Centisome position: 33.75

GC content: 48.13

Gene sequence:

>1947_bases
ATGGAACATAGCTTCTTAATCCAAGTACTACTTATGCTAGTGATTGCCATTGTGGCTATTGCAGTGCTGCGCCGTTTGGG
CTTACCCGCAATTCTGGCTTATTTACTCACTGGTGTCGTGAGTGGACCGAGCGGTTTTCATTGGTTTACCCAGCATCAAA
TGCAATCCGTCGCTGAACTTGGGGTGGTGCTGCTGATGTTTACTTTAGGCTTAGAGTTCTCCGTGCCGCGGCTGTGGGCC
ATGCGCCGTACCGTATTTGGCTTAGGCAGTGCACAGATGGTAATAACGACCCTGTTGACTATGGGCGTGGCCCTGTTGTG
TGGTCTTAATTGGGTAGAAGCCTTAGTTATCGGAGCTGCCATTGCCTTATCATCCACTGCCATCGTCCTTAAATTACTCA
ATGAACAGGGATGGTTGCGTCGTCGTCATGGTGAGCTATCCGTCAGTGTCTTATTATTCCAAGATTTAGCGGTTGTACCG
CTGTTGATCCTATTGCCATTATTGGCCAATGGCGGCGAACCTTTAATGGTCGCGGATATCGCCTTTGCCTTGCTCAAAGG
TATTTTGGCTTTTTTCCTCTTGATGGCTTTTGGTAAATGGGCATTGCCGCGGATTTTTGACGAAGTTGCCCGCTCCCGCT
CTAACGAACTCTTCGTGTTATCAACACTGGTGGTGGCCTTAGTGACAGGCGCATTTACCCAATGGTTGGGGTTATCTATG
GCGCTGGGGGCATTTATGGCGGGTATGCTACTAGGGGAAAGCCAGTACCGTCGCCAACTTGAGGCGGATATCCGGCCGTT
TCGCGATCTATTGATGGGGCTGTTTTTCATCTCCATCGGCATGATGCTGGACTTTACCTTAGTGATGCGTTTCTGGTGGC
AGATATTGTTGATCTTATTGGCCGTCGTACTAGGTAAGGCGCTGATCATTCATGGGATCTTAAGATTGGTTGGTGAACCA
TTTCGGATTGCGATGAGCACCGCCATGAGCCTAGCCCAAGTGGGCGAGTTCAGTTTTGTGGTGTTGGCTTTAGCGGTTAA
CTATCAACTTCTAGATAATGAAATCAGTACCATGTTAGTCATGGTCGCCGTGCTTTCCATGACGATAGCCCCGTGGCTAG
TTCGCCATAGTGTGGATATTGCTAAGTGGGTGTTAGGAATAAGGCAGGCAAAACATGTGGATGAAGCTGTGCCACTCTTG
ATGGATGAGCATGATCTGGTGATGATTTTTGGTTACGGCCGAGTCGGGCAGACCATTGCCCGTTTTTTGAAAACTGAAGC
TGTACCTTATCTGGTCATGGATCTCGATCCGACTCGTGTCTCTGAGGCGCGTCGTGCTGGCGAACCTATTTATTTTGGTG
ATGTGTGTAAACGGGCGATGTTAAAACAAATTGGCGTTAAGCAGGCCAAGATGATCGTCATCACTTTTTGTGAGTCGCGT
AGTTTAGAAGAAGTTCTACCCTTATGTAAGTTATTGGCGCCAGACGCTAAAATTTTAGTCAGAACTAAAGACGACAGTGA
TTTAGACTTACTGAAAAAAGCCGGTGCCAATCAAGTGATCCCAGAATCTTTGGAAGGCAGCCTGATGTTAGTGTCACAGG
TGTTACATCAATGTGGTGTGCCTTTGGCGCGCATTTTAAAACGCTTGGAGTCAGAGCGGCGTAATCATTATCAATTTTTA
CATGGCTTCTTTTCCGGTACTGAAACCGATTTCACCTTAGAGTCATTGCATGCGGTGGCGTTACCGCGTGGTGCAGATGC
GATTGGTAAAACCGTTGCTGAGATCATGTGGGAAACATTGAGGGTGGAGTTAAGGGCGATTCGTCGCAGTGGCGCAGAAG
TGGAACATCCTGAGCAGGACTGGGTTTTTCGTGCAGGGGATATCTTACTGATAGTGGGTAAACCACGAAGACTTGAGAAA
GCGGAAGCTAAACTGCTGCACGGCTAA

Upstream 100 bases:

>100_bases
TTTGTTAATAAAATTTGTCTTTGTTAGTCATTCAGTTAAGCTTAAAACTCAGCGTTAGTTTTTTTGACACGTCAGCATTT
TGACAGGAGTCATCGCCCCA

Downstream 100 bases:

>100_bases
AGGCTAAAGGTTAATGGTTAATGGTTAATGGTTAATGGTTAATGGTTAATGGTTAATGGTCAGCGTGAGTGATGGATGAA
AACGCAGATATAAAAATGCC

Product: potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MEHSFLIQVLLMLVIAIVAIAVLRRLGLPAILAYLLTGVVSGPSGFHWFTQHQMQSVAELGVVLLMFTLGLEFSVPRLWA
MRRTVFGLGSAQMVITTLLTMGVALLCGLNWVEALVIGAAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVP
LLILLPLLANGGEPLMVADIAFALLKGILAFFLLMAFGKWALPRIFDEVARSRSNELFVLSTLVVALVTGAFTQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMMLDFTLVMRFWWQILLILLAVVLGKALIIHGILRLVGEP
FRIAMSTAMSLAQVGEFSFVVLALAVNYQLLDNEISTMLVMVAVLSMTIAPWLVRHSVDIAKWVLGIRQAKHVDEAVPLL
MDEHDLVMIFGYGRVGQTIARFLKTEAVPYLVMDLDPTRVSEARRAGEPIYFGDVCKRAMLKQIGVKQAKMIVITFCESR
SLEEVLPLCKLLAPDAKILVRTKDDSDLDLLKKAGANQVIPESLEGSLMLVSQVLHQCGVPLARILKRLESERRNHYQFL
HGFFSGTETDFTLESLHAVALPRGADAIGKTVAEIMWETLRVELRAIRRSGAEVEHPEQDWVFRAGDILLIVGKPRRLEK
AEAKLLHG

Sequences:

>Translated_648_residues
MEHSFLIQVLLMLVIAIVAIAVLRRLGLPAILAYLLTGVVSGPSGFHWFTQHQMQSVAELGVVLLMFTLGLEFSVPRLWA
MRRTVFGLGSAQMVITTLLTMGVALLCGLNWVEALVIGAAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVP
LLILLPLLANGGEPLMVADIAFALLKGILAFFLLMAFGKWALPRIFDEVARSRSNELFVLSTLVVALVTGAFTQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMMLDFTLVMRFWWQILLILLAVVLGKALIIHGILRLVGEP
FRIAMSTAMSLAQVGEFSFVVLALAVNYQLLDNEISTMLVMVAVLSMTIAPWLVRHSVDIAKWVLGIRQAKHVDEAVPLL
MDEHDLVMIFGYGRVGQTIARFLKTEAVPYLVMDLDPTRVSEARRAGEPIYFGDVCKRAMLKQIGVKQAKMIVITFCESR
SLEEVLPLCKLLAPDAKILVRTKDDSDLDLLKKAGANQVIPESLEGSLMLVSQVLHQCGVPLARILKRLESERRNHYQFL
HGFFSGTETDFTLESLHAVALPRGADAIGKTVAEIMWETLRVELRAIRRSGAEVEHPEQDWVFRAGDILLIVGKPRRLEK
AEAKLLHG
>Mature_648_residues
MEHSFLIQVLLMLVIAIVAIAVLRRLGLPAILAYLLTGVVSGPSGFHWFTQHQMQSVAELGVVLLMFTLGLEFSVPRLWA
MRRTVFGLGSAQMVITTLLTMGVALLCGLNWVEALVIGAAIALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVP
LLILLPLLANGGEPLMVADIAFALLKGILAFFLLMAFGKWALPRIFDEVARSRSNELFVLSTLVVALVTGAFTQWLGLSM
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMMLDFTLVMRFWWQILLILLAVVLGKALIIHGILRLVGEP
FRIAMSTAMSLAQVGEFSFVVLALAVNYQLLDNEISTMLVMVAVLSMTIAPWLVRHSVDIAKWVLGIRQAKHVDEAVPLL
MDEHDLVMIFGYGRVGQTIARFLKTEAVPYLVMDLDPTRVSEARRAGEPIYFGDVCKRAMLKQIGVKQAKMIVITFCESR
SLEEVLPLCKLLAPDAKILVRTKDDSDLDLLKKAGANQVIPESLEGSLMLVSQVLHQCGVPLARILKRLESERRNHYQFL
HGFFSGTETDFTLESLHAVALPRGADAIGKTVAEIMWETLRVELRAIRRSGAEVEHPEQDWVFRAGDILLIVGKPRRLEK
AEAKLLHG

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=407, Percent_Identity=26.044226044226, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1789749, Length=545, Percent_Identity=35.5963302752294, Blast_Score=289, Evalue=5e-79,
Organism=Escherichia coli, GI1786232, Length=569, Percent_Identity=33.0404217926186, Blast_Score=277, Evalue=2e-75,
Organism=Escherichia coli, GI1786685, Length=523, Percent_Identity=30.019120458891, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322367, Length=409, Percent_Identity=22.7383863080685, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 71886; Mature: 71886

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHSFLIQVLLMLVIAIVAIAVLRRLGLPAILAYLLTGVVSGPSGFHWFTQHQMQSVAEL
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GVVLLMFTLGLEFSVPRLWAMRRTVFGLGSAQMVITTLLTMGVALLCGLNWVEALVIGAA
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVPLLILLPLLANGGEPLMVADI
HHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AFALLKGILAFFLLMAFGKWALPRIFDEVARSRSNELFVLSTLVVALVTGAFTQWLGLSM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMMLDFTLVMRFWWQILLILL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVVLGKALIIHGILRLVGEPFRIAMSTAMSLAQVGEFSFVVLALAVNYQLLDNEISTMLV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
MVAVLSMTIAPWLVRHSVDIAKWVLGIRQAKHVDEAVPLLMDEHDLVMIFGYGRVGQTIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
RFLKTEAVPYLVMDLDPTRVSEARRAGEPIYFGDVCKRAMLKQIGVKQAKMIVITFCESR
HHHHHCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCC
SLEEVLPLCKLLAPDAKILVRTKDDSDLDLLKKAGANQVIPESLEGSLMLVSQVLHQCGV
CHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHCCC
PLARILKRLESERRNHYQFLHGFFSGTETDFTLESLHAVALPRGADAIGKTVAEIMWETL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RVELRAIRRSGAEVEHPEQDWVFRAGDILLIVGKPRRLEKAEAKLLHG
HHHHHHHHHCCCCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHHCCC
>Mature Secondary Structure
MEHSFLIQVLLMLVIAIVAIAVLRRLGLPAILAYLLTGVVSGPSGFHWFTQHQMQSVAEL
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GVVLLMFTLGLEFSVPRLWAMRRTVFGLGSAQMVITTLLTMGVALLCGLNWVEALVIGAA
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IALSSTAIVLKLLNEQGWLRRRHGELSVSVLLFQDLAVVPLLILLPLLANGGEPLMVADI
HHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AFALLKGILAFFLLMAFGKWALPRIFDEVARSRSNELFVLSTLVVALVTGAFTQWLGLSM
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALGAFMAGMLLGESQYRRQLEADIRPFRDLLMGLFFISIGMMLDFTLVMRFWWQILLILL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVVLGKALIIHGILRLVGEPFRIAMSTAMSLAQVGEFSFVVLALAVNYQLLDNEISTMLV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
MVAVLSMTIAPWLVRHSVDIAKWVLGIRQAKHVDEAVPLLMDEHDLVMIFGYGRVGQTIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
RFLKTEAVPYLVMDLDPTRVSEARRAGEPIYFGDVCKRAMLKQIGVKQAKMIVITFCESR
HHHHHCCCCEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCC
SLEEVLPLCKLLAPDAKILVRTKDDSDLDLLKKAGANQVIPESLEGSLMLVSQVLHQCGV
CHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHCCC
PLARILKRLESERRNHYQFLHGFFSGTETDFTLESLHAVALPRGADAIGKTVAEIMWETL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RVELRAIRRSGAEVEHPEQDWVFRAGDILLIVGKPRRLEKAEAKLLHG
HHHHHHHHHCCCCCCCCCCCCEEECCCEEEEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA