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The map label for this gene is tilS [H]

Identifier: 160874614

GI number: 160874614

Start: 1802108

End: 1803583

Strand: Direct

Name: tilS [H]

Synonym: Sbal195_1497

Alternate gene names: 160874614

Gene position: 1802108-1803583 (Clockwise)

Preceding gene: 160874613

Following gene: 160874616

Centisome position: 33.7

GC content: 48.51

Gene sequence:

>1476_bases
ATGTCGGTTAAGTTTGATGAGCCATTGGCAGCTTGCGATCTCAGCGCGCATATTAGTGCTTTTTTGACGGCGCAGTCGTT
AGCGCCTGACTGCAAGTTAGTGCTGGCTTACAGTGGCGGTGTTGATTCCGAAGTTTTGGCCTTTGGCTTAAGTCAGTTTG
CGCGGCAACATCCCGAGTTTCATTATCAACTGGTGTATGTGCACCATGGCCTCAGTGATAACGCTGATTCTTGGGCGCAG
CATTGTCTTGAGCGCGGTCAACATTATGGTTTGCCCGTGACGGTTGAGCGAGTGACAGTTAATCGCGGACCCAGATTAAG
CCTTGAAGCCGAAGCACGAAAAGTCCGTTATCAAGCGATTGAACGGCATTTAAGTGCAAATGATGTGTTGCTGACGGCCC
ATCATGAAGATGATCAGCTGGAAACTATTCTGCTGGCGCTGAAACGTGGTCAGGGCCCGAAGGGGCTGGCGGCCATGGGG
CAAGTACAAGCATTAGCACAAGGCAGACAACTGCGGCCGTTATTGGATATTAGCCGTGAGCAAATTGAAGCCTACGCAAA
ACAGCAAGGCTTAGTGCATATCGAAGATGAAAGTAATCAAGACGATAAGTACGATCGTAATTTTTTACGTTTAGAAATCA
TTCCACGCTTGAAAGCGCGTTGGCCGAGTATTGCGACAACCGCGAGTCGCAGCGCGGCATTGTGCGCTAAGCAACAGGCA
ACGTTGGATGTGCAGGTCAGTGAACGTTTACCTTTATGGTTAACCCAAGCGCCTTACCATACTCAAAGTGTATTCAAGCT
CGATGGTTTTGCCGAGCAGTCAGCCGATTGGCAAGCATTACTCTTGCGGGGTTTTATCGAATCACAAGGTTTTGCCTTGC
CATCAAAGGTGCAGCTTAATCAGATCATCACTCAGCTTTTAACCGCGAAAGATGATGCCAAAGTGCATATTCGTGTCGGT
GAATGTGAAATCCGCCGCTTTGCTAATCAAGTGTATTTATTTAAGTATCAAGCTTGTCCGCGTCATGTTAAGCCTGCGAG
CTGGGCATTTCCTTCGCAGCTCCTAAATCCTTTAGTGCAGGGCGATGTTACTCAAGTGGACGTGACTCAATCAGACGTTA
CTCAATTGGATGTGACTCAATTAGACGTGAAACCTGCGGGTGTTACTTGTGGTGAGACGATACGTCTCAGTGCCTCTAGA
CAAGGGCCTAGAGCTCGCTTTCCTCGGCCAGATGAAGTGTTGAGTGTGCGATATCAACTTGCGGGGCAATATCGCTGTCA
TCCCCATTCCCGTGGCAAGGGCCGAGAATTAAAGAAACTGTGGCAAGAGTTTGCCGTTCCGCCTTGGGTACGTGAAAAGA
TCGGTTTCGTGTTCTATAACGAGCAACTGGTGATGGCGATTGGGCTGTGGGTTGAAAAAAGCTTTATTCTTGAGTTGAAT
GGACTCGGTTTGCAGGTCTCTCAAACTTCTGTTTAA

Upstream 100 bases:

>100_bases
ACTTGATGCTCTCGCTCGAATCGCTACTCGGGCCAAACAAGGTGAGAATTTTATTCTAATGTTGGCGAATGTCTTAGGTG
CAAACAGATGAAGTTAACCT

Downstream 100 bases:

>100_bases
GTGCTTGTTTAAGTGCCTGTTGGCGAAGCAAGGGGTTTAGATTGGGAGTTATCGCTGGAGTTTTTGTGAGTGGTTGCAGT
GTTATTCTTTTTAGTAGGAA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSVKFDEPLAACDLSAHISAFLTAQSLAPDCKLVLAYSGGVDSEVLAFGLSQFARQHPEFHYQLVYVHHGLSDNADSWAQ
HCLERGQHYGLPVTVERVTVNRGPRLSLEAEARKVRYQAIERHLSANDVLLTAHHEDDQLETILLALKRGQGPKGLAAMG
QVQALAQGRQLRPLLDISREQIEAYAKQQGLVHIEDESNQDDKYDRNFLRLEIIPRLKARWPSIATTASRSAALCAKQQA
TLDVQVSERLPLWLTQAPYHTQSVFKLDGFAEQSADWQALLLRGFIESQGFALPSKVQLNQIITQLLTAKDDAKVHIRVG
ECEIRRFANQVYLFKYQACPRHVKPASWAFPSQLLNPLVQGDVTQVDVTQSDVTQLDVTQLDVKPAGVTCGETIRLSASR
QGPRARFPRPDEVLSVRYQLAGQYRCHPHSRGKGRELKKLWQEFAVPPWVREKIGFVFYNEQLVMAIGLWVEKSFILELN
GLGLQVSQTSV

Sequences:

>Translated_491_residues
MSVKFDEPLAACDLSAHISAFLTAQSLAPDCKLVLAYSGGVDSEVLAFGLSQFARQHPEFHYQLVYVHHGLSDNADSWAQ
HCLERGQHYGLPVTVERVTVNRGPRLSLEAEARKVRYQAIERHLSANDVLLTAHHEDDQLETILLALKRGQGPKGLAAMG
QVQALAQGRQLRPLLDISREQIEAYAKQQGLVHIEDESNQDDKYDRNFLRLEIIPRLKARWPSIATTASRSAALCAKQQA
TLDVQVSERLPLWLTQAPYHTQSVFKLDGFAEQSADWQALLLRGFIESQGFALPSKVQLNQIITQLLTAKDDAKVHIRVG
ECEIRRFANQVYLFKYQACPRHVKPASWAFPSQLLNPLVQGDVTQVDVTQSDVTQLDVTQLDVKPAGVTCGETIRLSASR
QGPRARFPRPDEVLSVRYQLAGQYRCHPHSRGKGRELKKLWQEFAVPPWVREKIGFVFYNEQLVMAIGLWVEKSFILELN
GLGLQVSQTSV
>Mature_490_residues
SVKFDEPLAACDLSAHISAFLTAQSLAPDCKLVLAYSGGVDSEVLAFGLSQFARQHPEFHYQLVYVHHGLSDNADSWAQH
CLERGQHYGLPVTVERVTVNRGPRLSLEAEARKVRYQAIERHLSANDVLLTAHHEDDQLETILLALKRGQGPKGLAAMGQ
VQALAQGRQLRPLLDISREQIEAYAKQQGLVHIEDESNQDDKYDRNFLRLEIIPRLKARWPSIATTASRSAALCAKQQAT
LDVQVSERLPLWLTQAPYHTQSVFKLDGFAEQSADWQALLLRGFIESQGFALPSKVQLNQIITQLLTAKDDAKVHIRVGE
CEIRRFANQVYLFKYQACPRHVKPASWAFPSQLLNPLVQGDVTQVDVTQSDVTQLDVTQLDVKPAGVTCGETIRLSASRQ
GPRARFPRPDEVLSVRYQLAGQYRCHPHSRGKGRELKKLWQEFAVPPWVREKIGFVFYNEQLVMAIGLWVEKSFILELNG
LGLQVSQTSV

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=447, Percent_Identity=38.255033557047, Blast_Score=280, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 55166; Mature: 55034

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKFDEPLAACDLSAHISAFLTAQSLAPDCKLVLAYSGGVDSEVLAFGLSQFARQHPEF
CCCCCCCCHHHHCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCE
HYQLVYVHHGLSDNADSWAQHCLERGQHYGLPVTVERVTVNRGPRLSLEAEARKVRYQAI
EEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHH
ERHLSANDVLLTAHHEDDQLETILLALKRGQGPKGLAAMGQVQALAQGRQLRPLLDISRE
HHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
QIEAYAKQQGLVHIEDESNQDDKYDRNFLRLEIIPRLKARWPSIATTASRSAALCAKQQA
HHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECHHHCCCCCHHHHCCCHHHHHHHCCC
TLDVQVSERLPLWLTQAPYHTQSVFKLDGFAEQSADWQALLLRGFIESQGFALPSKVQLN
EEEEEECCCCCEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
QIITQLLTAKDDAKVHIRVGECEIRRFANQVYLFKYQACPRHVKPASWAFPSQLLNPLVQ
HHHHHHHCCCCCCEEEEEECHHHHHHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHC
GDVTQVDVTQSDVTQLDVTQLDVKPAGVTCGETIRLSASRQGPRARFPRPDEVLSVRYQL
CCCEEEEECHHHCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
AGQYRCHPHSRGKGRELKKLWQEFAVPPWVREKIGFVFYNEQLVMAIGLWVEKSFILELN
CCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHHCCEEEECCEEEEEEHHHHCCEEEEEEE
GLGLQVSQTSV
CCEEEEECCCC
>Mature Secondary Structure 
SVKFDEPLAACDLSAHISAFLTAQSLAPDCKLVLAYSGGVDSEVLAFGLSQFARQHPEF
CCCCCCCHHHHCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCE
HYQLVYVHHGLSDNADSWAQHCLERGQHYGLPVTVERVTVNRGPRLSLEAEARKVRYQAI
EEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHH
ERHLSANDVLLTAHHEDDQLETILLALKRGQGPKGLAAMGQVQALAQGRQLRPLLDISRE
HHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
QIEAYAKQQGLVHIEDESNQDDKYDRNFLRLEIIPRLKARWPSIATTASRSAALCAKQQA
HHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEECHHHCCCCCHHHHCCCHHHHHHHCCC
TLDVQVSERLPLWLTQAPYHTQSVFKLDGFAEQSADWQALLLRGFIESQGFALPSKVQLN
EEEEEECCCCCEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHH
QIITQLLTAKDDAKVHIRVGECEIRRFANQVYLFKYQACPRHVKPASWAFPSQLLNPLVQ
HHHHHHHCCCCCCEEEEEECHHHHHHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHC
GDVTQVDVTQSDVTQLDVTQLDVKPAGVTCGETIRLSASRQGPRARFPRPDEVLSVRYQL
CCCEEEEECHHHCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHH
AGQYRCHPHSRGKGRELKKLWQEFAVPPWVREKIGFVFYNEQLVMAIGLWVEKSFILELN
CCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHHCCEEEECCEEEEEEHHHHCCEEEEEEE
GLGLQVSQTSV
CCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]