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The map label for this gene is truC [H]

Identifier: 160874589

GI number: 160874589

Start: 1772425

End: 1773288

Strand: Direct

Name: truC [H]

Synonym: Sbal195_1472

Alternate gene names: 160874589

Gene position: 1772425-1773288 (Clockwise)

Preceding gene: 160874588

Following gene: 160874591

Centisome position: 33.15

GC content: 48.84

Gene sequence:

>864_bases
ATGAATCAAGATGATATCTGGCTTGAAACGCCTGAAAATGCACTGAATGCATCCCAAGAGGATGACTCAGAAGAGCAGGC
GCCTGAGATTGAAATTCTTTATGAAGATGAGCACTTAGTGGCAATTCATAAACCTGCAGGCTTATTGGTCCACCGCAGTT
ATTTAGCGCGTCGGGAGCGATTTTTTGCCATGCAGCTGACCCGCGACTTAGTCGGCTGTCATGTGTTCCCTGTTCATCGT
CTCGACAGGCCGACATCTGGCGTGCTTTTGTTTGCCAAGAGCAGCGAAGTCGCCAATGCACTGTGTGAGCAATTTGCCTC
CCATACGATTGAAAAACAATATTTGGCTTTAGTGCGCGGCAATATGCATGAGAGCGGTATTTTAGACTATGCGCTTAAGG
TTGAGTTGGATGAATTAGGCGATAAGAATGCCGATCCCAACAAAGCCGCGCAGGAAGCTGTGACGGCTTATGAGCCATTA
CTCAATACTGAAATTCCGTATCCCTCGGGCCGATATCCCACTAGCCGATTTGCACTGGTACGCTTGAGCCCTAAAACGGG
GCGTAAGCATCAGCTTAGAAGACATATGGCGCATTTACGTCACCCTATTGTCGGTGATACCACCCATGGTGACGGTAAGC
AAAATGCGTTTTTCCGCGAGCATTTTGGCATTAATCGCCTGTGGCTTATCGCGAAAAAGCTCAGCTTCACCCATCCAGTG
ACTCAAGTTCGGCTCAGTATAGAAACTGAGCTCGAGCCTCAGTGGGAAACCGTGTTTGCCGAGCTAGGTTGGGACGAGGC
GACCTTAAGTCAACAGGCTGAGTTGTTAATCGCCACGGATGAAATACCAGCTGGAAATGCGTAA

Upstream 100 bases:

>100_bases
ACGCAAATTGCGATAGCCCCTATGGCTGAACATGTTTGGCAGGATATGCCCCCGCGCCAAGCTCTGATTGGCGTATTGAA
TGAATTGGATATGTTGTTAA

Downstream 100 bases:

>100_bases
CTCATGAGCTTAACTCAGACAATCTCAACTGAGACAATCTGGACATAATTGGCCGTCAGCCAAGTGAGTGGCATCAAAAG
TCATTCGCTTGGCTTGGTTA

Product: pseudouridine synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNQDDIWLETPENALNASQEDDSEEQAPEIEILYEDEHLVAIHKPAGLLVHRSYLARRERFFAMQLTRDLVGCHVFPVHR
LDRPTSGVLLFAKSSEVANALCEQFASHTIEKQYLALVRGNMHESGILDYALKVELDELGDKNADPNKAAQEAVTAYEPL
LNTEIPYPSGRYPTSRFALVRLSPKTGRKHQLRRHMAHLRHPIVGDTTHGDGKQNAFFREHFGINRLWLIAKKLSFTHPV
TQVRLSIETELEPQWETVFAELGWDEATLSQQAELLIATDEIPAGNA

Sequences:

>Translated_287_residues
MNQDDIWLETPENALNASQEDDSEEQAPEIEILYEDEHLVAIHKPAGLLVHRSYLARRERFFAMQLTRDLVGCHVFPVHR
LDRPTSGVLLFAKSSEVANALCEQFASHTIEKQYLALVRGNMHESGILDYALKVELDELGDKNADPNKAAQEAVTAYEPL
LNTEIPYPSGRYPTSRFALVRLSPKTGRKHQLRRHMAHLRHPIVGDTTHGDGKQNAFFREHFGINRLWLIAKKLSFTHPV
TQVRLSIETELEPQWETVFAELGWDEATLSQQAELLIATDEIPAGNA
>Mature_287_residues
MNQDDIWLETPENALNASQEDDSEEQAPEIEILYEDEHLVAIHKPAGLLVHRSYLARRERFFAMQLTRDLVGCHVFPVHR
LDRPTSGVLLFAKSSEVANALCEQFASHTIEKQYLALVRGNMHESGILDYALKVELDELGDKNADPNKAAQEAVTAYEPL
LNTEIPYPSGRYPTSRFALVRLSPKTGRKHQLRRHMAHLRHPIVGDTTHGDGKQNAFFREHFGINRLWLIAKKLSFTHPV
TQVRLSIETELEPQWETVFAELGWDEATLSQQAELLIATDEIPAGNA

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs [H]

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family [H]

Homologues:

Organism=Homo sapiens, GI23308689, Length=182, Percent_Identity=31.8681318681319, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI17158025, Length=159, Percent_Identity=32.7044025157233, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI221316667, Length=186, Percent_Identity=32.7956989247312, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI221316663, Length=205, Percent_Identity=29.7560975609756, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789155, Length=235, Percent_Identity=54.8936170212766, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI1787327, Length=237, Percent_Identity=35.4430379746835, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786244, Length=221, Percent_Identity=33.0316742081448, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788946, Length=259, Percent_Identity=27.4131274131274, Blast_Score=87, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321608, Length=216, Percent_Identity=30.5555555555556, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320168, Length=195, Percent_Identity=29.2307692307692, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324506, Length=191, Percent_Identity=29.8429319371728, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583449, Length=177, Percent_Identity=27.683615819209, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583447, Length=177, Percent_Identity=27.683615819209, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583445, Length=177, Percent_Identity=27.683615819209, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921090, Length=178, Percent_Identity=28.0898876404494, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 32547; Mature: 32547

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS01129 PSI_RLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQDDIWLETPENALNASQEDDSEEQAPEIEILYEDEHLVAIHKPAGLLVHRSYLARRER
CCCCCEEEECCHHHHCCCCCCCCHHCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHH
FFAMQLTRDLVGCHVFPVHRLDRPTSGVLLFAKSSEVANALCEQFASHTIEKQYLALVRG
HHHHHHHHHHHCCEEEEHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHC
NMHESGILDYALKVELDELGDKNADPNKAAQEAVTAYEPLLNTEIPYPSGRYPTSRFALV
CCCCCCCEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
RLSPKTGRKHQLRRHMAHLRHPIVGDTTHGDGKQNAFFREHFGINRLWLIAKKLSFTHPV
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCHHEEHHHHHCCCCCC
TQVRLSIETELEPQWETVFAELGWDEATLSQQAELLIATDEIPAGNA
EEEEEEEECCCCCHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MNQDDIWLETPENALNASQEDDSEEQAPEIEILYEDEHLVAIHKPAGLLVHRSYLARRER
CCCCCEEEECCHHHHCCCCCCCCHHCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHH
FFAMQLTRDLVGCHVFPVHRLDRPTSGVLLFAKSSEVANALCEQFASHTIEKQYLALVRG
HHHHHHHHHHHCCEEEEHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHC
NMHESGILDYALKVELDELGDKNADPNKAAQEAVTAYEPLLNTEIPYPSGRYPTSRFALV
CCCCCCCEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
RLSPKTGRKHQLRRHMAHLRHPIVGDTTHGDGKQNAFFREHFGINRLWLIAKKLSFTHPV
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCHHEEHHHHHCCCCCC
TQVRLSIETELEPQWETVFAELGWDEATLSQQAELLIATDEIPAGNA
EEEEEEEECCCCCHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]