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The map label for this gene is pksE [H]

Identifier: 160874568

GI number: 160874568

Start: 1738578

End: 1740224

Strand: Reverse

Name: pksE [H]

Synonym: Sbal195_1450

Alternate gene names: 160874568

Gene position: 1740224-1738578 (Counterclockwise)

Preceding gene: 160874569

Following gene: 160874567

Centisome position: 32.54

GC content: 51.73

Gene sequence:

>1647_bases
ATGACTAGCCATACTCTAGATCAATTCAATAGTAATAACGAAAAACTTAGCCCTTGGCCGTGGCAAGTCAACGATGCAGA
GCTGAGCTTTGATATCGACTCATTAGGCAAAAAACTCAAAGATTTAAGCCAAGCCTGCTATTTAGTGAATCACAGCGAAA
AAGGCTTAGGCATAGCGCAAACGGCCGAAGTGACCACCAGTGACAGCCAAGCGCCACTAGGCTCACACCCTGTCAGCGCC
TTTGCCCCTGCCCTTGGCACCCAAAGTTTAGGCGACAGTAATTTTCGCCGCGTCCACGGCGTTAAATACGCTTACTACGC
CGGCGCTATGGCAAACGGTATTGCCTCGGAAGAACTGGTTATCGCCCTTGGCCAAGCGGGCATTTTGTGCTCATTTGGCG
CGGCGGGGTTAATTCCATCCCGTGTTGAAGCGGCCATTACCCGCATTCAAGCAGCGCTGCCCAATGGCCCTTACGCTTTT
AACTTAATTCACAGCCCAAGCGAGCCCGCATTAGAGCGCGGCAGCGTTGAGCTGTTTTTAAAACATAAAGTGCGCACGGT
CGAAGCCTCGGCATTTTTAGGTTTAACACCGCAAATCGTCTATTACCGCGCAGCAGGTTTGAGCCGCGACGAACATGGCG
AGATAGTCATTGGCAATAAAGTCATCGCAAAAATCAGCCGCACCGAAGTCGCGACTAAGTTTATGGAGCCGGCGCCTGCC
AAAATCCTCCAGCAATTAGTGAGTGATGGTCTTATCAGCCAAGATCAAATGGCGATGGCGCAACTGGTGCCAATGGCGGA
CGACATCACGGCCGAAGCCGACTCAGGCGGCCATACCGACAATCGTCCATTGGTCACACTATTGCCGACGATTTTGGCGC
TCAAAGATGAAATCCAAGCTAAGTATCAATATAAAACCCCCATCCGTGTGGGCGCAGGCGGCGGCGTCGGCACACCCGAT
GCAGCATTAGCCACCTTCAACATGGGTGCGGCCTTTATCGTCACAGGTTCAATCAACCAAGCCTGCGTCGAAGCGGGCGC
CAGCGAACACACCCGTAAGTTACTCGCCACCACAGAAATGGCCGATGTGACTATGGCGCCTGCCGCCGATATGTTCGAAA
TGGGCGTGAAATTACAAGTGGTTAAGCGCGGTACTTTGTTCCCGATGCGCGCCAATAAGCTTTATGAAATCTACACCCGT
TACGATTCGATTGATGCCATCCCTGCGGACGAGCGTAAAAAGCTCGAAGAGCAAGTGTTCCGCTCATCACTCGATGACAT
TTGGGCGGGCACTGTCGCCCACTTTAACGAGCGCGATCCTAAGCAAATCGAGCGCGCATTGGATAACCCAAAACGTAAGA
TGGCGTTGATTTTCCGCTGGTATTTGGGTCTGTCTAGCCGCTGGTCAAACACAGGTGAAGTCGGCCGCGAAATGGATTAC
CAAATCTGGGCAGGCCCAGCGCTGGGTGCATTTAACGCTTGGGCGAAAGGCAGTTATTTAGATGACTATAAAGCCCGTAA
TGCAGTAGATTTAGCCAAACATTTAATGGTGGGTGCGGCGTATCAATCTCGGATTAACTTGCTGTTATCCCAAGGCGCGA
GCATTCCGGTCACACTACTACGCTGGAAACCGCTGAATCGTTTTTAA

Upstream 100 bases:

>100_bases
TTGAATCACTTAAAAATAATCCTGCCGCTGCGTTGGCTTTTACACCCGAACTTTTCACTCAAGTAATAGCAACGCAGCAG
CAATAAAAACGAGACCCAAG

Downstream 100 bases:

>100_bases
GCACTACGGGACAACAGATACATCAAGTAGGGACTCAACTCCCTGCTTGATGCAGTCTTCTTCACTACCTCGTTAAATCG
AAACCGCCTACGTTATTGCC

Product: PfaD family protein

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MTSHTLDQFNSNNEKLSPWPWQVNDAELSFDIDSLGKKLKDLSQACYLVNHSEKGLGIAQTAEVTTSDSQAPLGSHPVSA
FAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELVIALGQAGILCSFGAAGLIPSRVEAAITRIQAALPNGPYAF
NLIHSPSEPALERGSVELFLKHKVRTVEASAFLGLTPQIVYYRAAGLSRDEHGEIVIGNKVIAKISRTEVATKFMEPAPA
KILQQLVSDGLISQDQMAMAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKYQYKTPIRVGAGGGVGTPD
AALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTR
YDSIDAIPADERKKLEEQVFRSSLDDIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
QIWAGPALGAFNAWAKGSYLDDYKARNAVDLAKHLMVGAAYQSRINLLLSQGASIPVTLLRWKPLNRF

Sequences:

>Translated_548_residues
MTSHTLDQFNSNNEKLSPWPWQVNDAELSFDIDSLGKKLKDLSQACYLVNHSEKGLGIAQTAEVTTSDSQAPLGSHPVSA
FAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELVIALGQAGILCSFGAAGLIPSRVEAAITRIQAALPNGPYAF
NLIHSPSEPALERGSVELFLKHKVRTVEASAFLGLTPQIVYYRAAGLSRDEHGEIVIGNKVIAKISRTEVATKFMEPAPA
KILQQLVSDGLISQDQMAMAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKYQYKTPIRVGAGGGVGTPD
AALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTR
YDSIDAIPADERKKLEEQVFRSSLDDIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
QIWAGPALGAFNAWAKGSYLDDYKARNAVDLAKHLMVGAAYQSRINLLLSQGASIPVTLLRWKPLNRF
>Mature_547_residues
TSHTLDQFNSNNEKLSPWPWQVNDAELSFDIDSLGKKLKDLSQACYLVNHSEKGLGIAQTAEVTTSDSQAPLGSHPVSAF
APALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELVIALGQAGILCSFGAAGLIPSRVEAAITRIQAALPNGPYAFN
LIHSPSEPALERGSVELFLKHKVRTVEASAFLGLTPQIVYYRAAGLSRDEHGEIVIGNKVIAKISRTEVATKFMEPAPAK
ILQQLVSDGLISQDQMAMAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKYQYKTPIRVGAGGGVGTPDA
ALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRY
DSIDAIPADERKKLEEQVFRSSLDDIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDYQ
IWAGPALGAFNAWAKGSYLDDYKARNAVDLAKHLMVGAAYQSRINLLLSQGASIPVTLLRWKPLNRF

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 59632; Mature: 59500

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHTLDQFNSNNEKLSPWPWQVNDAELSFDIDSLGKKLKDLSQACYLVNHSEKGLGIAQ
CCCCCCHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHEEEECCCCCCCEEE
TAEVTTSDSQAPLGSHPVSAFAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELV
ECEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEE
IALGQAGILCSFGAAGLIPSRVEAAITRIQAALPNGPYAFNLIHSPSEPALERGSVELFL
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCEEEEE
KHKVRTVEASAFLGLTPQIVYYRAAGLSRDEHGEIVIGNKVIAKISRTEVATKFMEPAPA
EHHHEEEEHHHEECCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHH
KILQQLVSDGLISQDQMAMAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQA
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KYQYKTPIRVGAGGGVGTPDAALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEM
EEEECCCEEEECCCCCCCCCHHEEEECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHH
ADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIDAIPADERKKLEEQVF
HHEEECCCHHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
RSSLDDIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
QIWAGPALGAFNAWAKGSYLDDYKARNAVDLAKHLMVGAAYQSRINLLLSQGASIPVTLL
EEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
RWKPLNRF
ECCCCCCC
>Mature Secondary Structure 
TSHTLDQFNSNNEKLSPWPWQVNDAELSFDIDSLGKKLKDLSQACYLVNHSEKGLGIAQ
CCCCCHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHEEEECCCCCCCEEE
TAEVTTSDSQAPLGSHPVSAFAPALGTQSLGDSNFRRVHGVKYAYYAGAMANGIASEELV
ECEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCCCCEE
IALGQAGILCSFGAAGLIPSRVEAAITRIQAALPNGPYAFNLIHSPSEPALERGSVELFL
EEECCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCCEEEEE
KHKVRTVEASAFLGLTPQIVYYRAAGLSRDEHGEIVIGNKVIAKISRTEVATKFMEPAPA
EHHHEEEEHHHEECCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHH
KILQQLVSDGLISQDQMAMAQLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQA
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KYQYKTPIRVGAGGGVGTPDAALATFNMGAAFIVTGSINQACVEAGASEHTRKLLATTEM
EEEECCCEEEECCCCCCCCCHHEEEECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHH
ADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIDAIPADERKKLEEQVF
HHEEECCCHHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHH
RSSLDDIWAGTVAHFNERDPKQIERALDNPKRKMALIFRWYLGLSSRWSNTGEVGREMDY
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCC
QIWAGPALGAFNAWAKGSYLDDYKARNAVDLAKHLMVGAAYQSRINLLLSQGASIPVTLL
EEECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
RWKPLNRF
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]