Click here to switch to the map view.

The map label for this gene is rotA [H]

Identifier: 160874474

GI number: 160874474

Start: 1612945

End: 1613574

Strand: Reverse

Name: rotA [H]

Synonym: Sbal195_1355

Alternate gene names: 160874474

Gene position: 1613574-1612945 (Counterclockwise)

Preceding gene: 160874484

Following gene: 160874467

Centisome position: 30.18

GC content: 45.87

Gene sequence:

>630_bases
ATGAAGAAGTTATCAGGATTACTGTTTGTCGGCTGCATAGCCTTAACCGGTTGTGAAGGAAGTACAGATGGTGATCTAAC
GCCGATGCCACCGACGCCCGATCCCACTCCGCCAGCCTTAGCTGCGGATGTGTGTTATTTGATGAAAACCACAAAAGGCG
ATATCACACTCGCCATTGACTTAACGAACATGCCTATCACAGGTAAAAACTTTAAGCAGTATGTTGATAAGTCTTTCTAT
AACGGCACCTTATTCCATAGAACCGTTAATAACTTTATGATCCAAGGCGGCGGTTTTACCACAGGGCTCAAAAGCAAACC
AGGCGATGCCCCCATCAAGAATGAAGCCGCAGTCGGTATTAGCAACAAACGTGGCACTATCTCCATGGCTCGCACGGATG
ACCCTAATTCTGCAACATCACAGTTCTTTATCAATGTGTTAGATAATCCACATTTGGATGCGTCTGCAAGTAGTTATGGA
TATGCCGTGTTTGGCAAAGTGGTTGACGGCATGGATGTGGCGGATCAGATCAGCATAGTACCAACAAAAAGTAGCGGTGG
TTTCGCCAACACGCCAGTACAAGAAATTCTGATTAACTCTGTGGCTGAAACAAGCTGTCCTGCTGCTTAA

Upstream 100 bases:

>100_bases
TTCATGGAGCCTGATATGCCCTCGCTTATCCGCCAGAGGCTCGCTCGATGGAACGCTAAAGCAGAAACTACCCAATGATG
ATTGAGAATAAGGATATGAA

Downstream 100 bases:

>100_bases
GTCTGTAAAAGACGCACAGCTAAACGACATCGAGTTAAAAGCAAAAAAGCACTGAATCTTCAGTGCTTTTTCATTTTAAA
AACTAATCGATTTTAACGTC

Product: cyclophilin type peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKKLSGLLFVGCIALTGCEGSTDGDLTPMPPTPDPTPPALAADVCYLMKTTKGDITLAIDLTNMPITGKNFKQYVDKSFY
NGTLFHRTVNNFMIQGGGFTTGLKSKPGDAPIKNEAAVGISNKRGTISMARTDDPNSATSQFFINVLDNPHLDASASSYG
YAVFGKVVDGMDVADQISIVPTKSSGGFANTPVQEILINSVAETSCPAA

Sequences:

>Translated_209_residues
MKKLSGLLFVGCIALTGCEGSTDGDLTPMPPTPDPTPPALAADVCYLMKTTKGDITLAIDLTNMPITGKNFKQYVDKSFY
NGTLFHRTVNNFMIQGGGFTTGLKSKPGDAPIKNEAAVGISNKRGTISMARTDDPNSATSQFFINVLDNPHLDASASSYG
YAVFGKVVDGMDVADQISIVPTKSSGGFANTPVQEILINSVAETSCPAA
>Mature_209_residues
MKKLSGLLFVGCIALTGCEGSTDGDLTPMPPTPDPTPPALAADVCYLMKTTKGDITLAIDLTNMPITGKNFKQYVDKSFY
NGTLFHRTVNNFMIQGGGFTTGLKSKPGDAPIKNEAAVGISNKRGTISMARTDDPNSATSQFFINVLDNPHLDASASSYG
YAVFGKVVDGMDVADQISIVPTKSSGGFANTPVQEILINSVAETSCPAA

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=165, Percent_Identity=32.1212121212121, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI22547215, Length=156, Percent_Identity=35.2564102564103, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI22547212, Length=156, Percent_Identity=34.6153846153846, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI7657473, Length=156, Percent_Identity=34.6153846153846, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI7706339, Length=155, Percent_Identity=30.3225806451613, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789763, Length=167, Percent_Identity=53.2934131736527, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI1786736, Length=157, Percent_Identity=50.9554140127389, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71980590, Length=137, Percent_Identity=35.036496350365, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71980594, Length=137, Percent_Identity=35.036496350365, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539496, Length=156, Percent_Identity=35.2564102564103, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71997782, Length=126, Percent_Identity=36.5079365079365, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17552780, Length=154, Percent_Identity=34.4155844155844, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17506311, Length=160, Percent_Identity=33.125, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6320510, Length=159, Percent_Identity=37.7358490566038, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321848, Length=150, Percent_Identity=35.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320359, Length=122, Percent_Identity=37.7049180327869, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922912, Length=135, Percent_Identity=38.5185185185185, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17986117, Length=138, Percent_Identity=31.8840579710145, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922376, Length=157, Percent_Identity=29.2993630573248, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 21935; Mature: 21935

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSGLLFVGCIALTGCEGSTDGDLTPMPPTPDPTPPALAADVCYLMKTTKGDITLAID
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
LTNMPITGKNFKQYVDKSFYNGTLFHRTVNNFMIQGGGFTTGLKSKPGDAPIKNEAAVGI
ECCCCCCCCHHHHHHHHHHCCCCHHEEECCCEEEECCCEECCCCCCCCCCCCCCCEEEEE
SNKRGTISMARTDDPNSATSQFFINVLDNPHLDASASSYGYAVFGKVVDGMDVADQISIV
CCCCCEEEEEECCCCCCCCHHEEEEECCCCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEE
PTKSSGGFANTPVQEILINSVAETSCPAA
EECCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKLSGLLFVGCIALTGCEGSTDGDLTPMPPTPDPTPPALAADVCYLMKTTKGDITLAID
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
LTNMPITGKNFKQYVDKSFYNGTLFHRTVNNFMIQGGGFTTGLKSKPGDAPIKNEAAVGI
ECCCCCCCCHHHHHHHHHHCCCCHHEEECCCEEEECCCEECCCCCCCCCCCCCCCEEEEE
SNKRGTISMARTDDPNSATSQFFINVLDNPHLDASASSYGYAVFGKVVDGMDVADQISIV
CCCCCEEEEEECCCCCCCCHHEEEEECCCCCCCCCCCCCCEEEEHHHHCCCCCCCCEEEE
PTKSSGGFANTPVQEILINSVAETSCPAA
EECCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]