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The map label for this gene is nadB [H]

Identifier: 160874390

GI number: 160874390

Start: 1509645

End: 1511258

Strand: Reverse

Name: nadB [H]

Synonym: Sbal195_1271

Alternate gene names: 160874390

Gene position: 1511258-1509645 (Counterclockwise)

Preceding gene: 160874403

Following gene: 160874379

Centisome position: 28.26

GC content: 49.38

Gene sequence:

>1614_bases
ATGAAACAAGTAGTTGAACACCAATCTGACATATTAGTCATAGGTAGCGGTGCCGCAGGTCTGACACTAGCTTTGCATCT
TGCTGAAAAAGCAAAAGTAATTCTGCTTTCAAAAGGCCCACTCTCGGAAGGGTCGACTTACTACGCCCAGGGCGGTATCG
CCTCCGTCTTCGACGAAAGTGACACTATCGAATCCCATGTTGCAGACACATTAATCGCAGGTGCGGGTTTATGCGACAAA
GACGTCGTCACTTTTACCGCTGAAAATGCTAAGAGTGCGATGCAATGGCTTATCGAATGTGGCGTCGCCTTCGATAAAGA
AGAAACCACTGACGGTAATGCAAAAGACGCACCTTACCATTTAACCCGTGAAGGCGGCCATAGCCACAGACGTATTCTGC
ACGCTGCCGATGCCACAGGTAAAGAAGTGCAAACAACGCTGCAAGAACGCGCACTCGCCCACCCCAATATTCAAGTTTTA
GAACGCTATAACGCCATCGACCTCATCACCACTCGTAAGTTAAATCGCCCAGGTAATCGTGTGTTAGGCGCTTATGTGTG
GAATCGCAACGAAGAACACGTCGAAACCGTTAAGGCCAAGTTTGTTGCGTTAGCAACAGGCGGTAGCTCTAAGGTTTATC
AATATACTTCAAACCCCGATGTCGCTAGCGGCGATGGTATTGCCATGGCATGGCGTGCAGGTTGCCGCGTGGCGAATATG
GAGTTCAACCAATTCCATCCGACATGTTTGTACCATGCCGATGCGCGCAACTTCTTACTCACCGAAGCCCTGCGTGGTGA
AGGTGCGTACCTGCGTCGTCCAGATGGCAGCCGTTTTATGCCGGACTTTGATGAACGCGCTGAACTCGCTCCCCGTGACA
TCGTTGCCCGCGCCATCGATTATGAGATGAAACGTCTCGGTGCCGATTGCGTGTATCTGGATATCAGCCATAAAGATGCT
GACTTTGTGATGAAGCATTTCCCGACCATCTATAGCCGTTGCATAGAGCTGGGAATTGATATCACTAAAGATGCGATCCC
TGTCGTGCCTGCCGCGCACTATACCTGCGGTGGTGTGATGACAGATATGCACGGTCAAACCGATCTCAATGGCCTCTATG
CCATTGGTGAAGTGGCCTATACGGGCTTACATGGCGCGAATCGTCTGGCGAGTAACTCGCTACTCGAATGTTTAGTGTTT
GCCCGTGCGGCGTCACATGATATAGAGAGCCAGCTGTATAAAATTCAAATGCCGGGACAAATTCCAGCATGGGATGAAAG
TAAAGTCTCTAACTCGGACGAAGAAGTGGTCATCGCCCATAACTGGCACGAACTGCGGTTATTCATGTGGGACTACGTCG
GGATTGTGCGCTCGGATAAACGTCTGGAGCGTGCACTGCGCCGCTGCCTGATGTTACAGCAAGAAATTCAGGAGTATTAC
AGTAACTTCAGAGTCAGCAATAACTTGCTCGAATTACGTAACTTAGTGCAAGTGGCCGAGTTGATTATTCGCTGTGCCAT
GGAACGTAAAGAAAGCCGTGGTCTGCATTTTAATATTGACCATCCAGAGAAATTAGACGCGCCGCGCCCAACAATTTTAT
TACCAGAAAAGTAA

Upstream 100 bases:

>100_bases
TCTAAAAAATATACAAAAAAGTAAGGTTAAAAGGAAAGTACCCATAATCCGTGAGTTGGTTTACCATAGTTGGCACTCTT
CAGTCACATCATGATACCCG

Downstream 100 bases:

>100_bases
CCGCTTAAGTTCTTATGTGTTTATAGGCTTAGGTTCTTATTTGGCTTGCTGAAAACAGACTAAATCTCGGTATGAGGTTT
AGTCTGTTTTATTTTAGCCC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKQVVEHQSDILVIGSGAAGLTLALHLAEKAKVILLSKGPLSEGSTYYAQGGIASVFDESDTIESHVADTLIAGAGLCDK
DVVTFTAENAKSAMQWLIECGVAFDKEETTDGNAKDAPYHLTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVL
ERYNAIDLITTRKLNRPGNRVLGAYVWNRNEEHVETVKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGADCVYLDISHKDA
DFVMKHFPTIYSRCIELGIDITKDAIPVVPAAHYTCGGVMTDMHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAASHDIESQLYKIQMPGQIPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHFNIDHPEKLDAPRPTILLPEK

Sequences:

>Translated_537_residues
MKQVVEHQSDILVIGSGAAGLTLALHLAEKAKVILLSKGPLSEGSTYYAQGGIASVFDESDTIESHVADTLIAGAGLCDK
DVVTFTAENAKSAMQWLIECGVAFDKEETTDGNAKDAPYHLTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVL
ERYNAIDLITTRKLNRPGNRVLGAYVWNRNEEHVETVKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGADCVYLDISHKDA
DFVMKHFPTIYSRCIELGIDITKDAIPVVPAAHYTCGGVMTDMHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAASHDIESQLYKIQMPGQIPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHFNIDHPEKLDAPRPTILLPEK
>Mature_537_residues
MKQVVEHQSDILVIGSGAAGLTLALHLAEKAKVILLSKGPLSEGSTYYAQGGIASVFDESDTIESHVADTLIAGAGLCDK
DVVTFTAENAKSAMQWLIECGVAFDKEETTDGNAKDAPYHLTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVL
ERYNAIDLITTRKLNRPGNRVLGAYVWNRNEEHVETVKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGADCVYLDISHKDA
DFVMKHFPTIYSRCIELGIDITKDAIPVVPAAHYTCGGVMTDMHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVF
ARAASHDIESQLYKIQMPGQIPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHFNIDHPEKLDAPRPTILLPEK

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=517, Percent_Identity=32.3017408123791, Blast_Score=193, Evalue=3e-49,
Organism=Escherichia coli, GI1788928, Length=536, Percent_Identity=71.2686567164179, Blast_Score=808, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=528, Percent_Identity=30.8712121212121, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1786942, Length=560, Percent_Identity=30.7142857142857, Blast_Score=185, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17550100, Length=571, Percent_Identity=32.0490367775832, Blast_Score=199, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17505833, Length=561, Percent_Identity=30.6595365418895, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71986328, Length=471, Percent_Identity=27.6008492569002, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=562, Percent_Identity=30.9608540925267, Blast_Score=207, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6322416, Length=570, Percent_Identity=30, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6322511, Length=493, Percent_Identity=25.7606490872211, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24663005, Length=565, Percent_Identity=30.6194690265487, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI17137288, Length=555, Percent_Identity=31.3513513513514, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24655642, Length=555, Percent_Identity=31.3513513513514, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24655647, Length=555, Percent_Identity=31.3513513513514, Blast_Score=197, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59895; Mature: 59895

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVVEHQSDILVIGSGAAGLTLALHLAEKAKVILLSKGPLSEGSTYYAQGGIASVFDES
CCCHHCCCCCEEEEECCCCCHHHEEEECCCCEEEEEECCCCCCCCEEEECCCCHHHCCCC
DTIESHVADTLIAGAGLCDKDVVTFTAENAKSAMQWLIECGVAFDKEETTDGNAKDAPYH
HHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
LTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVLERYNAIDLITTRKLNRPGNR
EECCCCCHHHHEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCCE
VLGAYVWNRNEEHVETVKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EEEEEEECCCHHHHHHHHHEEEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEECC
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAID
CCCCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCHHHHHHHHHH
YEMKRLGADCVYLDISHKDADFVMKHFPTIYSRCIELGIDITKDAIPVVPAAHYTCGGVM
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
TDMHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAASHDIESQLYKIQMPGQ
ECCCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEECCCC
IPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
CCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHFNIDHPEKLDAPRPTILLPEK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHCCCCCCEEECCCC
>Mature Secondary Structure
MKQVVEHQSDILVIGSGAAGLTLALHLAEKAKVILLSKGPLSEGSTYYAQGGIASVFDES
CCCHHCCCCCEEEEECCCCCHHHEEEECCCCEEEEEECCCCCCCCEEEECCCCHHHCCCC
DTIESHVADTLIAGAGLCDKDVVTFTAENAKSAMQWLIECGVAFDKEETTDGNAKDAPYH
HHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
LTREGGHSHRRILHAADATGKEVQTTLQERALAHPNIQVLERYNAIDLITTRKLNRPGNR
EECCCCCHHHHEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCCE
VLGAYVWNRNEEHVETVKAKFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANM
EEEEEEECCCHHHHHHHHHEEEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEECC
EFNQFHPTCLYHADARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAID
CCCCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCHHHHHHHHHH
YEMKRLGADCVYLDISHKDADFVMKHFPTIYSRCIELGIDITKDAIPVVPAAHYTCGGVM
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEE
TDMHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAASHDIESQLYKIQMPGQ
ECCCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEECCCC
IPAWDESKVSNSDEEVVIAHNWHELRLFMWDYVGIVRSDKRLERALRRCLMLQQEIQEYY
CCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SNFRVSNNLLELRNLVQVAELIIRCAMERKESRGLHFNIDHPEKLDAPRPTILLPEK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]