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The map label for this gene is purT

Identifier: 160874212

GI number: 160874212

Start: 1293129

End: 1294304

Strand: Reverse

Name: purT

Synonym: Sbal195_1092

Alternate gene names: 160874212

Gene position: 1294304-1293129 (Counterclockwise)

Preceding gene: 160874216

Following gene: 160874211

Centisome position: 24.2

GC content: 51.36

Gene sequence:

>1176_bases
ATGATAGGCACTCCCTACACTGAGGGCGCACGACGCGCCATGTTGCTCGGCTGCGGTGAACTTGGCAAAGAAGTGGCCAT
TGAACTGCAACGCTTAGGCGTTGAAGTGATTGGCGTTGACCGTTACGCCAACGCCCCCGCCATGCAAGTCGCGCATCGCT
CCCATGTGATCAATATGCTCGATGCCAACGCCCTGCGTGCAGTTATCGAGTTAGAAAAACCGCACTTAGTTATCCCAGAG
ATTGAAGCCATCGCTACCCAAACCTTAGTGGACATGGAAGCCGAAGGGGTGAATATCATCCCAACAGCTCGGGCAACTAA
GCTCACGATGGACAGAGAAGGGATTCGTCGCCTCGCCGCTGAGACCTTAGGTTTACCGACTTCTCCGTATTTTTTCTGCG
ATACTGAAACCGAATTTAATCAAGCAATTAGCGAAATCGGTGTGCCCTGTGTGGTTAAGCCAGTCATGAGCTCATCGGGC
AAAGGTCAGAGCGTTATTCGCGATATCGCCCTCAGCCATAAAGCATGGCAATACGCTCAAGAAGGTGGCCGCGCCGGCGG
CGGTCGAGTGATAGTCGAAGGTTTTATTCCCTTTGATTATGAAATCACCCTGTTAACCATTAGCGCAGTCAATGGCATTC
ATTTTTGTGCGCCAATTGGCCACAGACAAGAAGATGGCGATTACCGTGAGTCATGGCAGCCACAAGCCATGTCCGATGAA
GTGTTAGCAAAATCCCAAGCGATTGCGAGCAAAGTCGTCGAAGCCCTTGGCGGTTATGGATTATTTGGCGTAGAGCTGTT
TGTTAAAGGCCATGAAGTGTATTTCTCTGAGGTCTCACCAAGGCCACATGACACAGGTCTAGTGACACTCATCAGCCAAG
ACTTATCGGAATTTGCCCTGCATGTTAGAGCCATTCTGGGTCTGCCAATCCCCAATATCCATCAGCATGGGCCAAGCGCC
TCGGCGGTGATTTTGGCCGAAGGCACCTCAAGCAATATTCGCTACCAAGGTATTGCCGCTGCATTAGAGGCGGTCAACAC
TCAACTCAGATTATTTGCCAAACCCGACATTGATGGCCGCCGCCGCTTAGGTGTCGCGCTTGCCCGTGATATCGATATCG
ACAGCGCAGTCAGCAAAGCACTCGATAGCGCCAGTAAAGTGAAGGTCATCTTTTAA

Upstream 100 bases:

>100_bases
GTTTATTTACACCATGCTCTAATGTGCCGTTCGCGTTAAACTCATGGCTAAATTGCGCGCCATGACGCAATACCTACAAC
TGCTAATAAAGGAATCACAC

Downstream 100 bases:

>100_bases
GCGGGTTTAGCTGCTTAAAATTGGCTTCAAGCACAAGCTACAAATCAAAGGGAAATGCGCTTCAACGCCATTTCCCTTTT
TCAATGCACACAGTACAGCA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MIGTPYTEGARRAMLLGCGELGKEVAIELQRLGVEVIGVDRYANAPAMQVAHRSHVINMLDANALRAVIELEKPHLVIPE
IEAIATQTLVDMEAEGVNIIPTARATKLTMDREGIRRLAAETLGLPTSPYFFCDTETEFNQAISEIGVPCVVKPVMSSSG
KGQSVIRDIALSHKAWQYAQEGGRAGGGRVIVEGFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSDE
VLAKSQAIASKVVEALGGYGLFGVELFVKGHEVYFSEVSPRPHDTGLVTLISQDLSEFALHVRAILGLPIPNIHQHGPSA
SAVILAEGTSSNIRYQGIAAALEAVNTQLRLFAKPDIDGRRRLGVALARDIDIDSAVSKALDSASKVKVIF

Sequences:

>Translated_391_residues
MIGTPYTEGARRAMLLGCGELGKEVAIELQRLGVEVIGVDRYANAPAMQVAHRSHVINMLDANALRAVIELEKPHLVIPE
IEAIATQTLVDMEAEGVNIIPTARATKLTMDREGIRRLAAETLGLPTSPYFFCDTETEFNQAISEIGVPCVVKPVMSSSG
KGQSVIRDIALSHKAWQYAQEGGRAGGGRVIVEGFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSDE
VLAKSQAIASKVVEALGGYGLFGVELFVKGHEVYFSEVSPRPHDTGLVTLISQDLSEFALHVRAILGLPIPNIHQHGPSA
SAVILAEGTSSNIRYQGIAAALEAVNTQLRLFAKPDIDGRRRLGVALARDIDIDSAVSKALDSASKVKVIF
>Mature_391_residues
MIGTPYTEGARRAMLLGCGELGKEVAIELQRLGVEVIGVDRYANAPAMQVAHRSHVINMLDANALRAVIELEKPHLVIPE
IEAIATQTLVDMEAEGVNIIPTARATKLTMDREGIRRLAAETLGLPTSPYFFCDTETEFNQAISEIGVPCVVKPVMSSSG
KGQSVIRDIALSHKAWQYAQEGGRAGGGRVIVEGFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSDE
VLAKSQAIASKVVEALGGYGLFGVELFVKGHEVYFSEVSPRPHDTGLVTLISQDLSEFALHVRAILGLPIPNIHQHGPSA
SAVILAEGTSSNIRYQGIAAALEAVNTQLRLFAKPDIDGRRRLGVALARDIDIDSAVSKALDSASKVKVIF

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=68.1233933161954, Blast_Score=543, Evalue=1e-156,
Organism=Escherichia coli, GI1786733, Length=243, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=312, Percent_Identity=25.9615384615385, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_SHEB5 (A3D1A0)

Other databases:

- EMBL:   CP000563
- RefSeq:   YP_001049382.1
- ProteinModelPortal:   A3D1A0
- SMR:   A3D1A0
- STRING:   A3D1A0
- GeneID:   4841841
- GenomeReviews:   CP000563_GR
- KEGG:   sbl:Sbal_0989
- NMPDR:   fig|325240.3.peg.2359
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   A3D1A0
- ProtClustDB:   PRK09288
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42184; Mature: 42184

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 80-80 BINDING 112-112 BINDING 153-153 BINDING 201-201 BINDING 265-265 BINDING 277-277 BINDING 284-284 BINDING 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGTPYTEGARRAMLLGCGELGKEVAIELQRLGVEVIGVDRYANAPAMQVAHRSHVINML
CCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHH
DANALRAVIELEKPHLVIPEIEAIATQTLVDMEAEGVNIIPTARATKLTMDREGIRRLAA
HHHHHHHHHEECCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECHHHHHHHHH
ETLGLPTSPYFFCDTETEFNQAISEIGVPCVVKPVMSSSGKGQSVIRDIALSHKAWQYAQ
HHHCCCCCCEEEECCCHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EGGRAGGGRVIVEGFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSDE
HCCCCCCCEEEEEECCCCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCCCCCHH
VLAKSQAIASKVVEALGGYGLFGVELFVKGHEVYFSEVSPRPHDTGLVTLISQDLSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
HVRAILGLPIPNIHQHGPSASAVILAEGTSSNIRYQGIAAALEAVNTQLRLFAKPDIDGR
HHHHHHCCCCCCHHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHCCEEEEEECCCCCCC
RRLGVALARDIDIDSAVSKALDSASKVKVIF
HHHHHHHEECCCHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure
MIGTPYTEGARRAMLLGCGELGKEVAIELQRLGVEVIGVDRYANAPAMQVAHRSHVINML
CCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHH
DANALRAVIELEKPHLVIPEIEAIATQTLVDMEAEGVNIIPTARATKLTMDREGIRRLAA
HHHHHHHHHEECCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECHHHHHHHHH
ETLGLPTSPYFFCDTETEFNQAISEIGVPCVVKPVMSSSGKGQSVIRDIALSHKAWQYAQ
HHHCCCCCCEEEECCCHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EGGRAGGGRVIVEGFIPFDYEITLLTISAVNGIHFCAPIGHRQEDGDYRESWQPQAMSDE
HCCCCCCCEEEEEECCCCCEEEEEEEEEECCCEEEECCCCCCCCCCCHHHCCCCCCCCHH
VLAKSQAIASKVVEALGGYGLFGVELFVKGHEVYFSEVSPRPHDTGLVTLISQDLSEFAL
HHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
HVRAILGLPIPNIHQHGPSASAVILAEGTSSNIRYQGIAAALEAVNTQLRLFAKPDIDGR
HHHHHHCCCCCCHHCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHCCEEEEEECCCCCCC
RRLGVALARDIDIDSAVSKALDSASKVKVIF
HHHHHHHEECCCHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA