Click here to switch to the map view.

The map label for this gene is lptD [H]

Identifier: 160874202

GI number: 160874202

Start: 1279975

End: 1282272

Strand: Reverse

Name: lptD [H]

Synonym: Sbal195_1082

Alternate gene names: 160874202

Gene position: 1282272-1279975 (Counterclockwise)

Preceding gene: 160874209

Following gene: 160874201

Centisome position: 23.98

GC content: 44.91

Gene sequence:

>2298_bases
ATGCAGATCCGATACCTTCTGGCCCTGAGCCTGTTACCGCAATTAGTCCTGGCAGACGACTCTCCAACCGCTTCGGCATC
TCAATGCTTAATCGAACCGCCAGTACCCCGTATCGTATCTCAAACCCAAGGGATGAGTGCGGCTGAACTCAACAAAATTC
GTATCACGTCTGATAATACCAATGCCGAAATGGGCAAACAGGCGATTTTCCGTGGTGATGTGGTTTTTAGCCAAGGCGAT
AGACACATAGCGGCCGATGAGGCGATTCTAGATCAAGCGACCGAACAGTTTGATGCTAATGGTAATTTAGTCTTCCAAGA
CAGTAACTTCACTGTTACCGCCGAGTCATTACAAGCTCAAATGCGCTCCAATCGTGCCACCCTTAAAGGGGCGCAATATT
GGCTGCATGGACAACAGGTCCATGGCGATGCAGAAAAGCTGCAAATTACCATCAATAACAATTTAATTTTAACCAATACT
AACTTTACCACTTGTCCACCCGACAATGTCTCTTGGCTGCTCGAAGCCGAGAAAATCAAGATTAACAGTGAAGAAGAATG
GGGCGAAATTTGGAATGCCAAGTTAAGAGTCGGCGGCGTGCCTGTATTCTACATTCCCTACATGACAGTGCCGGTTTCTG
ATAAACGTAAGTCGGGTTTCCTCTACCCAAGCTTTAGTACCAGTACCACCAATGGCGTCGAAATAGCTGCACCCTATTAC
TGGAACATTGCCCCAGAATATGATTTAACCTTTACGCCTAACTATATGTCTAACCGTGGTTTATTTACTAAGACTGAGTT
TGGTTATTTAGCAGGCGAGGCTCAGAAAGGCCGAGTCAATTTTGAATATCTAGGCAACGATCAATTGATCAGTGGCAGCC
CTAATCGCTACCTGTACAACTGGCAACACCAAGGGTCAATCGACAAGAATTGGCGTGTATTAGCTAATTTTACCGACGTG
TCAGACAACAACTATTTCAATGACTTAAAGTCAGATGTTAACCGTGCTACCGACAACCAGCTGTCGCGCATAGGTGAAAT
CAGCTACTTCGAAAGTAACTGGGATATCAGCACCCGGGTTCAAGATATTAAAGTACTCGGTGAAGACGAAAAACCGTATC
AGGTGATGCCTCAGGTCAACTTTAACTATCGCGCCGCCGATTTTTGGAACAATTTAGATTTTGGCTTTAATTCAGAGCTG
ACGAATTTTGCCCATCAAGACAACAATGTGAACACGGCGACGCGTTTACATATGTCACCGAGCGTTACCCTACCAATTCA
CGGACCTTCGGGCTCACTCACCAGCCAAGTGAAGCTGATGCAAACTAATTATTGGCAACAGAATAACAATCCTAATGAAG
AACTAGACGACACGGTAAGCCGTACCATTCCGCAGGTACGGATTAATGGTCAGATTAACTTCGAGCGTTTTACTGAGCTT
TTCGATGAAAACTATCGCCAAACGCTGGAACCGCAATTCCAATATCTTTATGTTGGCTACGAAGACCAACGTGGCATAGG
TATTTATGATACGGCCCAATTACAGGATGACTATTTTGGTCTATTCCGTGATCGCCGTTTCTCGGGACTGGATCGTATTT
CCGATGCGAACCAAGTCACGCTAGGTTTAACAACACGCTTGTTCGACGATCACAACCAAGAAGCGACTAAATTCAGCCTA
GGGCAAATTTTTTACTTGCAAGACAGTAAAGTCGGCTACGAAGACAATATTTTTGAATCAAATCAATCGACATCTGTACT
CGCTGCCGAGTTAGATACCCGTTTGACGAATGATTGGTATATGGGTGCGGCGATTCAGTACGACACCAACACCAGTGAAA
ATAAAAAAACGGAAGTCACCTTAGACTTCAGACCCGAAGCGAACAAATTGCTGCAATTTAGCTATCGCTACGTCCCCGAT
TTATTGAACTCCAATACCAATGATACTGTGAACATCTCCCAAGCGGGTGTTCGTGGCGCTTGGCCCATCAGTGACGATCT
CTACTTCGTGGGTAATTGGTATTATGACTTAAACGAAAGCCGCAGCATTGAAACCTACACAGGTATTCAATACGAATCAT
GTTGTTATGCGGTGCGATTTAGTTATCACTACCGCATTAAAACTAACTATGACGACAACATAGGCTCTGCTGCTATCGAC
TCCCGTGAGCAGTTTGAAAGCGGCTTCTACCTGAATTTAGTCATTAAAGGACTCGGTGGTTCTGGCCCATTAGGCGTAAC
CGATATGCTCAATGACGGCCTGTTTAACTACAGAAAACCGCTTTATCTGAGGAATTAG

Upstream 100 bases:

>100_bases
AAGAAATTGAGAGATTTATTAAGCGGCGTTGCGTTTGTAAATTGCAACTTTCCCCATCCATACTTGTCCAGAATAAGAAA
TCATAATTACTGACCTAAAG

Downstream 100 bases:

>100_bases
CAAAGAATTGTGATAGATTGCTGAACCTCAACAAGGACTGAGAGTCCAAACAGGTTAATGAGTGCTTTATCGTTTGCCTT
TTGCTAACGCTAATAAAAGC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 765; Mature: 765

Protein sequence:

>765_residues
MQIRYLLALSLLPQLVLADDSPTASASQCLIEPPVPRIVSQTQGMSAAELNKIRITSDNTNAEMGKQAIFRGDVVFSQGD
RHIAADEAILDQATEQFDANGNLVFQDSNFTVTAESLQAQMRSNRATLKGAQYWLHGQQVHGDAEKLQITINNNLILTNT
NFTTCPPDNVSWLLEAEKIKINSEEEWGEIWNAKLRVGGVPVFYIPYMTVPVSDKRKSGFLYPSFSTSTTNGVEIAAPYY
WNIAPEYDLTFTPNYMSNRGLFTKTEFGYLAGEAQKGRVNFEYLGNDQLISGSPNRYLYNWQHQGSIDKNWRVLANFTDV
SDNNYFNDLKSDVNRATDNQLSRIGEISYFESNWDISTRVQDIKVLGEDEKPYQVMPQVNFNYRAADFWNNLDFGFNSEL
TNFAHQDNNVNTATRLHMSPSVTLPIHGPSGSLTSQVKLMQTNYWQQNNNPNEELDDTVSRTIPQVRINGQINFERFTEL
FDENYRQTLEPQFQYLYVGYEDQRGIGIYDTAQLQDDYFGLFRDRRFSGLDRISDANQVTLGLTTRLFDDHNQEATKFSL
GQIFYLQDSKVGYEDNIFESNQSTSVLAAELDTRLTNDWYMGAAIQYDTNTSENKKTEVTLDFRPEANKLLQFSYRYVPD
LLNSNTNDTVNISQAGVRGAWPISDDLYFVGNWYYDLNESRSIETYTGIQYESCCYAVRFSYHYRIKTNYDDNIGSAAID
SREQFESGFYLNLVIKGLGGSGPLGVTDMLNDGLFNYRKPLYLRN

Sequences:

>Translated_765_residues
MQIRYLLALSLLPQLVLADDSPTASASQCLIEPPVPRIVSQTQGMSAAELNKIRITSDNTNAEMGKQAIFRGDVVFSQGD
RHIAADEAILDQATEQFDANGNLVFQDSNFTVTAESLQAQMRSNRATLKGAQYWLHGQQVHGDAEKLQITINNNLILTNT
NFTTCPPDNVSWLLEAEKIKINSEEEWGEIWNAKLRVGGVPVFYIPYMTVPVSDKRKSGFLYPSFSTSTTNGVEIAAPYY
WNIAPEYDLTFTPNYMSNRGLFTKTEFGYLAGEAQKGRVNFEYLGNDQLISGSPNRYLYNWQHQGSIDKNWRVLANFTDV
SDNNYFNDLKSDVNRATDNQLSRIGEISYFESNWDISTRVQDIKVLGEDEKPYQVMPQVNFNYRAADFWNNLDFGFNSEL
TNFAHQDNNVNTATRLHMSPSVTLPIHGPSGSLTSQVKLMQTNYWQQNNNPNEELDDTVSRTIPQVRINGQINFERFTEL
FDENYRQTLEPQFQYLYVGYEDQRGIGIYDTAQLQDDYFGLFRDRRFSGLDRISDANQVTLGLTTRLFDDHNQEATKFSL
GQIFYLQDSKVGYEDNIFESNQSTSVLAAELDTRLTNDWYMGAAIQYDTNTSENKKTEVTLDFRPEANKLLQFSYRYVPD
LLNSNTNDTVNISQAGVRGAWPISDDLYFVGNWYYDLNESRSIETYTGIQYESCCYAVRFSYHYRIKTNYDDNIGSAAID
SREQFESGFYLNLVIKGLGGSGPLGVTDMLNDGLFNYRKPLYLRN
>Mature_765_residues
MQIRYLLALSLLPQLVLADDSPTASASQCLIEPPVPRIVSQTQGMSAAELNKIRITSDNTNAEMGKQAIFRGDVVFSQGD
RHIAADEAILDQATEQFDANGNLVFQDSNFTVTAESLQAQMRSNRATLKGAQYWLHGQQVHGDAEKLQITINNNLILTNT
NFTTCPPDNVSWLLEAEKIKINSEEEWGEIWNAKLRVGGVPVFYIPYMTVPVSDKRKSGFLYPSFSTSTTNGVEIAAPYY
WNIAPEYDLTFTPNYMSNRGLFTKTEFGYLAGEAQKGRVNFEYLGNDQLISGSPNRYLYNWQHQGSIDKNWRVLANFTDV
SDNNYFNDLKSDVNRATDNQLSRIGEISYFESNWDISTRVQDIKVLGEDEKPYQVMPQVNFNYRAADFWNNLDFGFNSEL
TNFAHQDNNVNTATRLHMSPSVTLPIHGPSGSLTSQVKLMQTNYWQQNNNPNEELDDTVSRTIPQVRINGQINFERFTEL
FDENYRQTLEPQFQYLYVGYEDQRGIGIYDTAQLQDDYFGLFRDRRFSGLDRISDANQVTLGLTTRLFDDHNQEATKFSL
GQIFYLQDSKVGYEDNIFESNQSTSVLAAELDTRLTNDWYMGAAIQYDTNTSENKKTEVTLDFRPEANKLLQFSYRYVPD
LLNSNTNDTVNISQAGVRGAWPISDDLYFVGNWYYDLNESRSIETYTGIQYESCCYAVRFSYHYRIKTNYDDNIGSAAID
SREQFESGFYLNLVIKGLGGSGPLGVTDMLNDGLFNYRKPLYLRN

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=791, Percent_Identity=33.7547408343869, Blast_Score=441, Evalue=1e-125,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 87314; Mature: 87314

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRYLLALSLLPQLVLADDSPTASASQCLIEPPVPRIVSQTQGMSAAELNKIRITSDNT
CEEHHHHHHHHHHHEEEECCCCCCCCCCEEECCCCHHHHHHHCCCCHHHCCEEEEECCCC
NAEMGKQAIFRGDVVFSQGDRHIAADEAILDQATEQFDANGNLVFQDSNFTVTAESLQAQ
CHHHHHHHEEECCEEEECCCCEEEHHHHHHHHHHHHHCCCCCEEEECCCEEEEHHHHHHH
MRSNRATLKGAQYWLHGQQVHGDAEKLQITINNNLILTNTNFTTCPPDNVSWLLEAEKIK
HHCCCCEEECCEEEEECCEECCCCEEEEEEECCCEEEECCCCCCCCCCCCEEEEEEEEEE
INSEEEWGEIWNAKLRVGGVPVFYIPYMTVPVSDKRKSGFLYPSFSTSTTNGVEIAAPYY
ECCCHHHHHHCCCEEEECCEEEEEECEEEEECCCCCCCCEEEECCCCCCCCCEEEECCEE
WNIAPEYDLTFTPNYMSNRGLFTKTEFGYLAGEAQKGRVNFEYLGNDQLISGSPNRYLYN
ECCCCCCCEEECCCCCCCCCCEEECCCCEEECCCCCCEEEEEEECCCEEECCCCCCEEEE
WQHQGSIDKNWRVLANFTDVSDNNYFNDLKSDVNRATDNQLSRIGEISYFESNWDISTRV
EECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEE
QDIKVLGEDEKPYQVMPQVNFNYRAADFWNNLDFGFNSELTNFAHQDNNVNTATRLHMSP
EEEEEECCCCCCEEEECCCCCCEEEHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECC
SVTLPIHGPSGSLTSQVKLMQTNYWQQNNNPNEELDDTVSRTIPQVRINGQINFERFTEL
CEEEEEECCCCCCCCEEEEEEHHCCCCCCCCCHHHHHHHHHCCCEEEECCEECHHHHHHH
FDENYRQTLEPQFQYLYVGYEDQRGIGIYDTAQLQDDYFGLFRDRRFSGLDRISDANQVT
HHHHHHHHCCCCEEEEEEEECCCCCCCEEECHHCCCHHHHHHHHCCCCCHHHCCCCCEEE
LGLTTRLFDDHNQEATKFSLGQIFYLQDSKVGYEDNIFESNQSTSVLAAELDTRLTNDWY
EEEEEEECCCCCCCCCEEECEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEE
MGAAIQYDTNTSENKKTEVTLDFRPEANKLLQFSYRYVPDLLNSNTNDTVNISQAGVRGA
EEEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
WPISDDLYFVGNWYYDLNESRSIETYTGIQYESCCYAVRFSYHYRIKTNYDDNIGSAAID
CCCCCCEEEEEEEEEECCCCCCEEEEECCCHHCEEEEEEEEEEEEEECCCCCCCCCHHCC
SREQFESGFYLNLVIKGLGGSGPLGVTDMLNDGLFNYRKPLYLRN
CHHHHCCCEEEEEEEEECCCCCCCCHHHHHHCCHHHCCCCEEECC
>Mature Secondary Structure
MQIRYLLALSLLPQLVLADDSPTASASQCLIEPPVPRIVSQTQGMSAAELNKIRITSDNT
CEEHHHHHHHHHHHEEEECCCCCCCCCCEEECCCCHHHHHHHCCCCHHHCCEEEEECCCC
NAEMGKQAIFRGDVVFSQGDRHIAADEAILDQATEQFDANGNLVFQDSNFTVTAESLQAQ
CHHHHHHHEEECCEEEECCCCEEEHHHHHHHHHHHHHCCCCCEEEECCCEEEEHHHHHHH
MRSNRATLKGAQYWLHGQQVHGDAEKLQITINNNLILTNTNFTTCPPDNVSWLLEAEKIK
HHCCCCEEECCEEEEECCEECCCCEEEEEEECCCEEEECCCCCCCCCCCCEEEEEEEEEE
INSEEEWGEIWNAKLRVGGVPVFYIPYMTVPVSDKRKSGFLYPSFSTSTTNGVEIAAPYY
ECCCHHHHHHCCCEEEECCEEEEEECEEEEECCCCCCCCEEEECCCCCCCCCEEEECCEE
WNIAPEYDLTFTPNYMSNRGLFTKTEFGYLAGEAQKGRVNFEYLGNDQLISGSPNRYLYN
ECCCCCCCEEECCCCCCCCCCEEECCCCEEECCCCCCEEEEEEECCCEEECCCCCCEEEE
WQHQGSIDKNWRVLANFTDVSDNNYFNDLKSDVNRATDNQLSRIGEISYFESNWDISTRV
EECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEE
QDIKVLGEDEKPYQVMPQVNFNYRAADFWNNLDFGFNSELTNFAHQDNNVNTATRLHMSP
EEEEEECCCCCCEEEECCCCCCEEEHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECC
SVTLPIHGPSGSLTSQVKLMQTNYWQQNNNPNEELDDTVSRTIPQVRINGQINFERFTEL
CEEEEEECCCCCCCCEEEEEEHHCCCCCCCCCHHHHHHHHHCCCEEEECCEECHHHHHHH
FDENYRQTLEPQFQYLYVGYEDQRGIGIYDTAQLQDDYFGLFRDRRFSGLDRISDANQVT
HHHHHHHHCCCCEEEEEEEECCCCCCCEEECHHCCCHHHHHHHHCCCCCHHHCCCCCEEE
LGLTTRLFDDHNQEATKFSLGQIFYLQDSKVGYEDNIFESNQSTSVLAAELDTRLTNDWY
EEEEEEECCCCCCCCCEEECEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEE
MGAAIQYDTNTSENKKTEVTLDFRPEANKLLQFSYRYVPDLLNSNTNDTVNISQAGVRGA
EEEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
WPISDDLYFVGNWYYDLNESRSIETYTGIQYESCCYAVRFSYHYRIKTNYDDNIGSAAID
CCCCCCEEEEEEEEEECCCCCCEEEEECCCHHCEEEEEEEEEEEEEECCCCCCCCCHHCC
SREQFESGFYLNLVIKGLGGSGPLGVTDMLNDGLFNYRKPLYLRN
CHHHHCCCEEEEEEEEECCCCCCCCHHHHHHCCHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]