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The map label for this gene is malE [H]

Identifier: 159900976

GI number: 159900976

Start: 5707502

End: 5708737

Strand: Direct

Name: malE [H]

Synonym: Haur_4463

Alternate gene names: 159900976

Gene position: 5707502-5708737 (Clockwise)

Preceding gene: 159900971

Following gene: 159900977

Centisome position: 89.93

GC content: 51.62

Gene sequence:

>1236_bases
ATGAGGGCAGTATGGCGGTATTTTAGCCTTTTTATGATTGGGATTCTCATTGTTGCTGGTTGTAGCACGACAAGCTATGA
TAACACTTTGTTAACCCCAGGGGCTACCGCTAATCCTCCCGCACGCACGCAACTGGTCATTTGGCATGGAGCCGATGCCC
AACGGAGTGAACTTTTCACCCGATTGCTGTTAGAATATCAACGTGAGCATCCAAACGTTGTCATTCAAATCGTCAATCGT
GGGGCAAACCTGCTACACGATTATCGTGCGGCCCTGCTTGAAGGCACACCACCAGACCTGATCTGGCTCAACGAAAATCG
TTGGGTAGGGGAACTGGCAGATCAACAGCTTATCATCGATCTGACAGAACGACTAAGCGACGAGAACCTTGAGTCAATTG
CGCCAGCTGCGCTTGATGGTGCCCGCTATGGCGAGAAATTATATGGCTTGCCATTGACGCTAGATCTACCTGTGCTGTAC
TATAATCGTGCAAACTTTGTGAGCACACCGCCGCAAAGCACTGCTGAATGGCTTGAGATCGCTCGCGGGTTTAGCGATGA
TCAAGGACAGTACGGATTAGCGTATAATTTATCGCTATACTTTACCCAACCCTACCTCCCAGCCTTCGGAGGCGCAATCT
TCGATACTACTGGCGAGGTCGTGCTGGGAACCCAAAGCTATACCCCAACATTACAGTGGTTGACGTGGGTTGACGAATTA
GCCCAAGACCCACGCTTGTTAGCCCGTGATGATCATCGACTGGTAGCTCGCAGCGTGAGCCAAAACAGTGCGATCATGAC
GATTGACTGGGCAGATCAAATCGGAACCTATCGTCAATTGTGGGGGGAGAACGTTGGCGTGCAACCATTGCCACGCCTGA
GCCAAACCGGCCAAGAACCGCAACCCTTTGTTCGGAGCAGCGTGCTTGTGATCAGCCCACGCAGCACTGAACAACAGCAA
AACGCCGCGCTTGACGTGATGCGGTTTCTTGTGGAGATGAAAGCGCAAACGGCTTTTCAAGCCGCTGATATACCAAGCGT
CCGAATCGACTTAGCCAGTGTCGATCCACTTTACACTCAAATTCAACTGGCGGTTAGTCGAGCCAGCGCTTGGCCCACCA
CCCTTCGTTTCAACAACGGATGGGATATCCTGATCGCTTTGGTGCGTAATAGCTTAAACGGCGCACCCTTGGAAGAAAGT
ATCGCGAATGCCGATCGCCTGCTACGGAGCGAGTAG

Upstream 100 bases:

>100_bases
GTCAGTAATGCCTGAGCATGGTATACTTTTGGTGATTCGTTGGCGTTTTATGCCTTTATAATCAGATAAAACGCAATTAA
CGAAAGAACGGGATATACCC

Downstream 100 bases:

>100_bases
CCAACCCAGCATATTACAGTAACCGCTTGACAAGCAAGGGCTAAATTAGCCCGTTGTGGTGCGATCTGTAGTTGATATTT
GGCAAAAGATGTAGTAGAAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MRAVWRYFSLFMIGILIVAGCSTTSYDNTLLTPGATANPPARTQLVIWHGADAQRSELFTRLLLEYQREHPNVVIQIVNR
GANLLHDYRAALLEGTPPDLIWLNENRWVGELADQQLIIDLTERLSDENLESIAPAALDGARYGEKLYGLPLTLDLPVLY
YNRANFVSTPPQSTAEWLEIARGFSDDQGQYGLAYNLSLYFTQPYLPAFGGAIFDTTGEVVLGTQSYTPTLQWLTWVDEL
AQDPRLLARDDHRLVARSVSQNSAIMTIDWADQIGTYRQLWGENVGVQPLPRLSQTGQEPQPFVRSSVLVISPRSTEQQQ
NAALDVMRFLVEMKAQTAFQAADIPSVRIDLASVDPLYTQIQLAVSRASAWPTTLRFNNGWDILIALVRNSLNGAPLEES
IANADRLLRSE

Sequences:

>Translated_411_residues
MRAVWRYFSLFMIGILIVAGCSTTSYDNTLLTPGATANPPARTQLVIWHGADAQRSELFTRLLLEYQREHPNVVIQIVNR
GANLLHDYRAALLEGTPPDLIWLNENRWVGELADQQLIIDLTERLSDENLESIAPAALDGARYGEKLYGLPLTLDLPVLY
YNRANFVSTPPQSTAEWLEIARGFSDDQGQYGLAYNLSLYFTQPYLPAFGGAIFDTTGEVVLGTQSYTPTLQWLTWVDEL
AQDPRLLARDDHRLVARSVSQNSAIMTIDWADQIGTYRQLWGENVGVQPLPRLSQTGQEPQPFVRSSVLVISPRSTEQQQ
NAALDVMRFLVEMKAQTAFQAADIPSVRIDLASVDPLYTQIQLAVSRASAWPTTLRFNNGWDILIALVRNSLNGAPLEES
IANADRLLRSE
>Mature_411_residues
MRAVWRYFSLFMIGILIVAGCSTTSYDNTLLTPGATANPPARTQLVIWHGADAQRSELFTRLLLEYQREHPNVVIQIVNR
GANLLHDYRAALLEGTPPDLIWLNENRWVGELADQQLIIDLTERLSDENLESIAPAALDGARYGEKLYGLPLTLDLPVLY
YNRANFVSTPPQSTAEWLEIARGFSDDQGQYGLAYNLSLYFTQPYLPAFGGAIFDTTGEVVLGTQSYTPTLQWLTWVDEL
AQDPRLLARDDHRLVARSVSQNSAIMTIDWADQIGTYRQLWGENVGVQPLPRLSQTGQEPQPFVRSSVLVISPRSTEQQQ
NAALDVMRFLVEMKAQTAFQAADIPSVRIDLASVDPLYTQIQLAVSRASAWPTTLRFNNGWDILIALVRNSLNGAPLEES
IANADRLLRSE

Specific function: Involved in an abc transport system for maltotriose [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45991; Mature: 45991

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVWRYFSLFMIGILIVAGCSTTSYDNTLLTPGATANPPARTQLVIWHGADAQRSELFT
CCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCHHHHHHH
RLLLEYQREHPNVVIQIVNRGANLLHDYRAALLEGTPPDLIWLNENRWVGELADQQLIID
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHCCCHHHHH
LTERLSDENLESIAPAALDGARYGEKLYGLPLTLDLPVLYYNRANFVSTPPQSTAEWLEI
HHHHCCCCCHHHHCHHHHHHHHHCHHHCCCCEEEECCEEEECCCCCCCCCCCHHHHHHHH
ARGFSDDQGQYGLAYNLSLYFTQPYLPAFGGAIFDTTGEVVLGTQSYTPTLQWLTWVDEL
HCCCCCCCCCEEEEEEEEEEEECCCCHHHCCEEECCCCCEEECCCCCCCHHHHHHHHHHH
AQDPRLLARDDHRLVARSVSQNSAIMTIDWADQIGTYRQLWGENVGVQPLPRLSQTGQEP
HCCCCEECCCCHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCHHHHCCCCC
QPFVRSSVLVISPRSTEQQQNAALDVMRFLVEMKAQTAFQAADIPSVRIDLASVDPLYTQ
CCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
IQLAVSRASAWPTTLRFNNGWDILIALVRNSLNGAPLEESIANADRLLRSE
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRAVWRYFSLFMIGILIVAGCSTTSYDNTLLTPGATANPPARTQLVIWHGADAQRSELFT
CCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEEEECCCCHHHHHHH
RLLLEYQREHPNVVIQIVNRGANLLHDYRAALLEGTPPDLIWLNENRWVGELADQQLIID
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHCCCHHHHH
LTERLSDENLESIAPAALDGARYGEKLYGLPLTLDLPVLYYNRANFVSTPPQSTAEWLEI
HHHHCCCCCHHHHCHHHHHHHHHCHHHCCCCEEEECCEEEECCCCCCCCCCCHHHHHHHH
ARGFSDDQGQYGLAYNLSLYFTQPYLPAFGGAIFDTTGEVVLGTQSYTPTLQWLTWVDEL
HCCCCCCCCCEEEEEEEEEEEECCCCHHHCCEEECCCCCEEECCCCCCCHHHHHHHHHHH
AQDPRLLARDDHRLVARSVSQNSAIMTIDWADQIGTYRQLWGENVGVQPLPRLSQTGQEP
HCCCCEECCCCHHHHHHHHCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCCHHHHCCCCC
QPFVRSSVLVISPRSTEQQQNAALDVMRFLVEMKAQTAFQAADIPSVRIDLASVDPLYTQ
CCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
IQLAVSRASAWPTTLRFNNGWDILIALVRNSLNGAPLEESIANADRLLRSE
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]