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The map label for this gene is lysX [H]

Identifier: 159899823

GI number: 159899823

Start: 4171352

End: 4172215

Strand: Direct

Name: lysX [H]

Synonym: Haur_3306

Alternate gene names: 159899823

Gene position: 4171352-4172215 (Clockwise)

Preceding gene: 159899822

Following gene: 159899824

Centisome position: 65.73

GC content: 48.61

Gene sequence:

>864_bases
ATGCGTGTAGGTATGTTATGTTCCCGAATTCGGGTTGAAGAAAAGCTGCTGATCACTGAGTTTGAGCGGCGCAATGTCCA
ATTTGAGCGGATTGATGATGATCAGACATGGTTTGATTTGAATGCTTTGCAAGCCAATCGCCAAGTGCGCGAACAATTTC
CTGATGTGATTATTGAGCGTTCGTTGCACCATGGCCGAGCGTTGTACACACTCAAAACCTTGAACGATGCTGGCATTCCA
ACCGTCAATAATTATGATGTGGCATTAACCTGTGGCGATAAATTTTTGACTACCCAAGCCTTATTATGCAACGGCGTGCC
ATCGCCCCGTTGTTTACTGGCTTACACTCAAGATTCAGCGCTCGAAGCGATTGAAAGTTTGGGCTATCCGGTAGTGTTGA
AGCCAGTAATTGGCTCGTGGGGGCGCTTGGTCTCAAAGATCAACGATCGCGAAGCTGCCGAGGCGGTGCTTGAGCATCGC
GATACCTTGGGCAATTATCAGCATGCCATTTTCTATATTCAAGAATATGTCAACAAACCAGGTGGTCGCGATATTCGGGC
GTTTGTGGTTGGCGATGAGTGCATTGCGGCGATTTATCGCACCAGCGGACACTGGATCACCAATACGGCTCGCGGCGGCC
AAGCATCAAACTGCCCAATCACGCCAGCCTTAGCCGATATTTGTATTGGCGCGGCCAATGCAGTTGGTGGCGGCGTAGTT
GCAATCGATGTGTTCGAGACTGCCGAAGGTCGTTATTTGGTCAATGAAGTCAATTACACCATGGAATTTCGCAACAGCAT
CAGCACAACTGGAGTGAATATTCCCGAACGAATTGTTGATTATGTGCTAGCTCAAGCGCAATAG

Upstream 100 bases:

>100_bases
GATTGCGGCGCAGAACTCGAAGTTGTCAGCCTGAACCCAGTTGTCTTGGAATTAGCACCCGAAGTCGAAGAAGACTGGGG
CGAGTAAGGGAGTTATGTCA

Downstream 100 bases:

>100_bases
GCGGCGGGCTGTAGCCATTTGAGGTTGTTATGCTTTCAGTTTCGATAGTTGGTGGCTCGGGCTATGTCGGTGGTGAATTG
CTGCGTTTATTGTTGGGGCA

Product: lysine biosynthesis enzyme LysX

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MRVGMLCSRIRVEEKLLITEFERRNVQFERIDDDQTWFDLNALQANRQVREQFPDVIIERSLHHGRALYTLKTLNDAGIP
TVNNYDVALTCGDKFLTTQALLCNGVPSPRCLLAYTQDSALEAIESLGYPVVLKPVIGSWGRLVSKINDREAAEAVLEHR
DTLGNYQHAIFYIQEYVNKPGGRDIRAFVVGDECIAAIYRTSGHWITNTARGGQASNCPITPALADICIGAANAVGGGVV
AIDVFETAEGRYLVNEVNYTMEFRNSISTTGVNIPERIVDYVLAQAQ

Sequences:

>Translated_287_residues
MRVGMLCSRIRVEEKLLITEFERRNVQFERIDDDQTWFDLNALQANRQVREQFPDVIIERSLHHGRALYTLKTLNDAGIP
TVNNYDVALTCGDKFLTTQALLCNGVPSPRCLLAYTQDSALEAIESLGYPVVLKPVIGSWGRLVSKINDREAAEAVLEHR
DTLGNYQHAIFYIQEYVNKPGGRDIRAFVVGDECIAAIYRTSGHWITNTARGGQASNCPITPALADICIGAANAVGGGVV
AIDVFETAEGRYLVNEVNYTMEFRNSISTTGVNIPERIVDYVLAQAQ
>Mature_287_residues
MRVGMLCSRIRVEEKLLITEFERRNVQFERIDDDQTWFDLNALQANRQVREQFPDVIIERSLHHGRALYTLKTLNDAGIP
TVNNYDVALTCGDKFLTTQALLCNGVPSPRCLLAYTQDSALEAIESLGYPVVLKPVIGSWGRLVSKINDREAAEAVLEHR
DTLGNYQHAIFYIQEYVNKPGGRDIRAFVVGDECIAAIYRTSGHWITNTARGGQASNCPITPALADICIGAANAVGGGVV
AIDVFETAEGRYLVNEVNYTMEFRNSISTTGVNIPERIVDYVLAQAQ

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of 'Glu-54' of lysW [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=253, Percent_Identity=28.8537549407115, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1787076, Length=193, Percent_Identity=34.1968911917098, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 31869; Mature: 31869

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGMLCSRIRVEEKLLITEFERRNVQFERIDDDQTWFDLNALQANRQVREQFPDVIIER
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHH
SLHHGRALYTLKTLNDAGIPTVNNYDVALTCGDKFLTTQALLCNGVPSPRCLLAYTQDSA
HHCCCCEEEEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEECCHHH
LEAIESLGYPVVLKPVIGSWGRLVSKINDREAAEAVLEHRDTLGNYQHAIFYIQEYVNKP
HHHHHHCCCCEEEHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
GGRDIRAFVVGDECIAAIYRTSGHWITNTARGGQASNCPITPALADICIGAANAVGGGVV
CCCCEEEEEECHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEE
AIDVFETAEGRYLVNEVNYTMEFRNSISTTGVNIPERIVDYVLAQAQ
EEEEEECCCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRVGMLCSRIRVEEKLLITEFERRNVQFERIDDDQTWFDLNALQANRQVREQFPDVIIER
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHH
SLHHGRALYTLKTLNDAGIPTVNNYDVALTCGDKFLTTQALLCNGVPSPRCLLAYTQDSA
HHCCCCEEEEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEECCHHH
LEAIESLGYPVVLKPVIGSWGRLVSKINDREAAEAVLEHRDTLGNYQHAIFYIQEYVNKP
HHHHHHCCCCEEEHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
GGRDIRAFVVGDECIAAIYRTSGHWITNTARGGQASNCPITPALADICIGAANAVGGGVV
CCCCEEEEEECHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEE
AIDVFETAEGRYLVNEVNYTMEFRNSISTTGVNIPERIVDYVLAQAQ
EEEEEECCCCCEEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12963379 [H]