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The map label for this gene is leuC

Identifier: 159899819

GI number: 159899819

Start: 4168134

End: 4169399

Strand: Direct

Name: leuC

Synonym: Haur_3302

Alternate gene names: 159899819

Gene position: 4168134-4169399 (Clockwise)

Preceding gene: 159899818

Following gene: 159899820

Centisome position: 65.68

GC content: 57.03

Gene sequence:

>1266_bases
ATGGGTCAGACATTTGCCGAGCAAATTTTGGGGCATGCTTCCGGTCGCAGCGACGTGCAAGCAGGCGATATGGTGGTAGT
CAACGTCGATTTGGTGATGATGCACGATAGCCTCTCGCCTAGCATTATCGAAACCTTGCACAACGAATTAGGCGCTGAAC
GCGTGTGGGATCGCGACAAAGTTGCAGTGGTAATCGACCACGTTGCCCCAGCCGCCACCGTGCGCCAAGCCGAGCAGCAA
CAACAAGTGCGGCGTTGGGTCGCCCAACAAGGCATCAGCCATTTGTTTGATGTTGGTCGCGGCATCTCGCACCCCGTGTT
GATCGAAGAAGGGTTAGTGCAACCGGGCATGCTGGTGGTTGGCAGCGATTCGCATAGCACTGGCTATGGTGCAGCAGCGG
CCTTTGGCTCAGGTATGGGCACGACCGATATCGCCTTAGCACTGGCGACTGGCCAAACTTGGTTTCGCGTCCCAGAAACT
GTGCGGGTCAATGCGGTTGGCAATTTTCAACCAGGCGTGAGCGTCAAGGATTTTGGTTTGTGGGCGGCTCGCACGCTCCG
CGCTGATGGAGCAACCTATCAAAGCGTCGAGTGGCACGGGGTTGATTTTCTTTCGTGGCGCGAACGTATGACCTTGGCAA
CTTTATCAATTGAAGTTGGAGCCAAGGCCGGAATCGTTGCCCCAACTGGCTTGGGCGCTGAACATCCCGTGCCAGAATGG
TTGAGGGTTGAGGCCGATGCCAGCTACAGCCGCGTTGTCGAATGCGATTTGAGCACGCTCGAACCGCAAGTTAGCGTACC
GCATTATGTCGATAACGTAGTCGATTTGGCTGATGTCGGGCGGGTCGCGGTTGATGTCGTCTATCTTGGCACCTGCACCA
ATGGCCACTACGAAGATATGGCCGCCGCCGCCAGCATTCTCAAGGGACGGCGTTTGGCTCCAAATGTGCGAATGATTGTC
GTACCTGCTTCGAGCGAAAGTCTGCATCGCGCCGCCAGCGATGGCACATTAGCTACCTTGTTGGCGGCTGGGGCAACCAT
CGGTACACCTGGTTGTGGCGCATGCATTGGTCGCCATATGGGCGTGTTAGCGCCCGATGAAGTCTGCGTTTTCACTGGCA
ATCGCAACTTCCGCGGACGAATGGGCAGCCCAGGAGCCAATATCTACTTGGCCTCGCCTGAAGTTGCCGCCGCCACCGCC
GTCACGGGCTACATCACCCACCCGCGCAATGTGCTCGATAGCACTGAGCAAGCAGTTTTCGCCTAA

Upstream 100 bases:

>100_bases
CTAACGATTTTTCAAGCTGGCAATTGAAATTGCTATTTCAATGCCCTAGCTTGCTATACCTTGATGTGCCATTTTTCAGC
TATTTTTGGGAGTTTTCGTA

Downstream 100 bases:

>100_bases
TTTTTGCAATTGCACAGGAGTCAGCACTATGCCACGGATTTGGGTTTTTGGAAATGATGTCAACACCGACCAGATGGTTC
CAGGCCGTTATGCTCCCTAC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MGQTFAEQILGHASGRSDVQAGDMVVVNVDLVMMHDSLSPSIIETLHNELGAERVWDRDKVAVVIDHVAPAATVRQAEQQ
QQVRRWVAQQGISHLFDVGRGISHPVLIEEGLVQPGMLVVGSDSHSTGYGAAAAFGSGMGTTDIALALATGQTWFRVPET
VRVNAVGNFQPGVSVKDFGLWAARTLRADGATYQSVEWHGVDFLSWRERMTLATLSIEVGAKAGIVAPTGLGAEHPVPEW
LRVEADASYSRVVECDLSTLEPQVSVPHYVDNVVDLADVGRVAVDVVYLGTCTNGHYEDMAAAASILKGRRLAPNVRMIV
VPASSESLHRAASDGTLATLLAAGATIGTPGCGACIGRHMGVLAPDEVCVFTGNRNFRGRMGSPGANIYLASPEVAAATA
VTGYITHPRNVLDSTEQAVFA

Sequences:

>Translated_421_residues
MGQTFAEQILGHASGRSDVQAGDMVVVNVDLVMMHDSLSPSIIETLHNELGAERVWDRDKVAVVIDHVAPAATVRQAEQQ
QQVRRWVAQQGISHLFDVGRGISHPVLIEEGLVQPGMLVVGSDSHSTGYGAAAAFGSGMGTTDIALALATGQTWFRVPET
VRVNAVGNFQPGVSVKDFGLWAARTLRADGATYQSVEWHGVDFLSWRERMTLATLSIEVGAKAGIVAPTGLGAEHPVPEW
LRVEADASYSRVVECDLSTLEPQVSVPHYVDNVVDLADVGRVAVDVVYLGTCTNGHYEDMAAAASILKGRRLAPNVRMIV
VPASSESLHRAASDGTLATLLAAGATIGTPGCGACIGRHMGVLAPDEVCVFTGNRNFRGRMGSPGANIYLASPEVAAATA
VTGYITHPRNVLDSTEQAVFA
>Mature_420_residues
GQTFAEQILGHASGRSDVQAGDMVVVNVDLVMMHDSLSPSIIETLHNELGAERVWDRDKVAVVIDHVAPAATVRQAEQQQ
QVRRWVAQQGISHLFDVGRGISHPVLIEEGLVQPGMLVVGSDSHSTGYGAAAAFGSGMGTTDIALALATGQTWFRVPETV
RVNAVGNFQPGVSVKDFGLWAARTLRADGATYQSVEWHGVDFLSWRERMTLATLSIEVGAKAGIVAPTGLGAEHPVPEWL
RVEADASYSRVVECDLSTLEPQVSVPHYVDNVVDLADVGRVAVDVVYLGTCTNGHYEDMAAAASILKGRRLAPNVRMIVV
PASSESLHRAASDGTLATLLAAGATIGTPGCGACIGRHMGVLAPDEVCVFTGNRNFRGRMGSPGANIYLASPEVAAATAV
TGYITHPRNVLDSTEQAVFA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=371, Percent_Identity=27.4932614555256, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786259, Length=386, Percent_Identity=33.160621761658, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI2367097, Length=460, Percent_Identity=25, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI32564738, Length=365, Percent_Identity=26.8493150684932, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=26.8493150684932, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25149342, Length=282, Percent_Identity=27.3049645390071, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6320440, Length=455, Percent_Identity=31.2087912087912, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321429, Length=435, Percent_Identity=27.816091954023, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=338, Percent_Identity=30.4733727810651, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322261, Length=342, Percent_Identity=27.4853801169591, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28571643, Length=338, Percent_Identity=29.585798816568, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161076999, Length=371, Percent_Identity=26.9541778975741, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281365315, Length=371, Percent_Identity=26.9541778975741, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17864292, Length=371, Percent_Identity=26.9541778975741, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_HERA2 (A9B805)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001546066.1
- ProteinModelPortal:   A9B805
- SMR:   A9B805
- GeneID:   5735172
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_3302
- HOGENOM:   HBG330745
- OMA:   EGTKIDQ
- ProtClustDB:   PRK00402
- BioCyc:   HAUR316274:HAUR_3302-MONOMER
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 44523; Mature: 44392

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTFAEQILGHASGRSDVQAGDMVVVNVDLVMMHDSLSPSIIETLHNELGAERVWDRDK
CCHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCHHHCCCCCC
VAVVIDHVAPAATVRQAEQQQQVRRWVAQQGISHLFDVGRGISHPVLIEEGLVQPGMLVV
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEE
GSDSHSTGYGAAAAFGSGMGTTDIALALATGQTWFRVPETVRVNAVGNFQPGVSVKDFGL
ECCCCCCCCCHHHHHCCCCCCCCEEEEEECCCEEEECCCEEEEEEEECCCCCCCEECCCH
WAARTLRADGATYQSVEWHGVDFLSWRERMTLATLSIEVGAKAGIVAPTGLGAEHPVPEW
HHHHEEECCCCEEECEEECCCCHHHHHHHEEEEEEEEEECCCCCEECCCCCCCCCCCCHH
LRVEADASYSRVVECDLSTLEPQVSVPHYVDNVVDLADVGRVAVDVVYLGTCTNGHYEDM
EEEECCCCCCEEEEECHHHCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHH
AAAASILKGRRLAPNVRMIVVPASSESLHRAASDGTLATLLAAGATIGTPGCGACIGRHM
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHC
GVLAPDEVCVFTGNRNFRGRMGSPGANIYLASPEVAAATAVTGYITHPRNVLDSTEQAVF
CCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHEEEEECCCHHHHCCHHHHHC
A
C
>Mature Secondary Structure 
GQTFAEQILGHASGRSDVQAGDMVVVNVDLVMMHDSLSPSIIETLHNELGAERVWDRDK
CHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCHHHCCCCCC
VAVVIDHVAPAATVRQAEQQQQVRRWVAQQGISHLFDVGRGISHPVLIEEGLVQPGMLVV
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEE
GSDSHSTGYGAAAAFGSGMGTTDIALALATGQTWFRVPETVRVNAVGNFQPGVSVKDFGL
ECCCCCCCCCHHHHHCCCCCCCCEEEEEECCCEEEECCCEEEEEEEECCCCCCCEECCCH
WAARTLRADGATYQSVEWHGVDFLSWRERMTLATLSIEVGAKAGIVAPTGLGAEHPVPEW
HHHHEEECCCCEEECEEECCCCHHHHHHHEEEEEEEEEECCCCCEECCCCCCCCCCCCHH
LRVEADASYSRVVECDLSTLEPQVSVPHYVDNVVDLADVGRVAVDVVYLGTCTNGHYEDM
EEEECCCCCCEEEEECHHHCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHH
AAAASILKGRRLAPNVRMIVVPASSESLHRAASDGTLATLLAAGATIGTPGCGACIGRHM
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHC
GVLAPDEVCVFTGNRNFRGRMGSPGANIYLASPEVAAATAVTGYITHPRNVLDSTEQAVF
CCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHEEEEECCCHHHHCCHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA