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The map label for this gene is lys20 [H]

Identifier: 159899818

GI number: 159899818

Start: 4166904

End: 4168037

Strand: Direct

Name: lys20 [H]

Synonym: Haur_3301

Alternate gene names: 159899818

Gene position: 4166904-4168037 (Clockwise)

Preceding gene: 159899817

Following gene: 159899819

Centisome position: 65.66

GC content: 55.11

Gene sequence:

>1134_bases
ATGACACGGCATTTTGCAATTGTTGATACGACATTACGCGAAGGCGAACAATGGGCCAATGCTCATTTCAGCAGTGAAGA
TCGCCTCGCCATCGCCAATTTGCTGGTTCAGTTTGGCGTTGAATACATCGAAATGACCTCTCCCTGCGCCTCGCCGCAAA
GTACGGCAGATTTGCGCAGCATCGCAGCCTTGCCGCTCGGCAACACCAAGCTGCTGACCCACACCCGTTGTAACCTTGAT
GACGTGCGGCTCGCTGCTGAATGTGGCGTGGCTGGCGTAAATATCCTCTTTGGCACATCACCCTATCTGCGCCAATGGAG
CCATGGTCGCTCAATTGAAGCGATTTTGGAAGAAGTTGGCCCAGTTGTGCGTTTCCTCCAAGAACGAAATATCGAAGTTC
GGTTCTCCTGCGAAGATTCATTCCGCACCCCACTGCCCGATTTATTGCGCGTCTACCAAGCTGTCGATTTGCTTGGCGTG
CAGCGGGTTGGCATCGCCGATACGGTGGGCATTGCCACGCCACGCGATGTTGAACGTGTGGTTGGTGCAGTCCGCAATGC
AGTTCGCTGCGACATTGAATTTCATGGCCACAACGATGGCGGCTGTGCAATTGCCAATGCCTATGCAGCGCTCGAAGCAG
GCGCAACCCACATCGATACGACCATTCTTGGCATTGGCGAACGCAACGGCATCGCCGCGCTCAGCGGCTTAATTGCCCGC
CTCTACCTCTCCGAACCTGAAAGTGTTGCAGGCTATGCCTTGCCAATGTTGGCCCAACTTGATCACACAGTTGCGGCAAT
CTTGGGCATTCAAGTGCCATTCAATGCCTGTATCACCAGCGAAACCGCCTTTGCTCACAAGGCTGGCCTGCATACCAAGG
CTGTTTTGGCCAATCCCAGCACCTATGAAGCGATCGATCCCAAGGCTTTTGGGCGCGAACGCAATGTGCTGATTGGTCAT
CGCCTGACTGGTCGCCATGCCTTGCAAAGCCGCGCCAACACCCTAGGTTTGGATCTGAGCGACACCACGATTATCGCGAT
TGCAGCCCAACTCAAAGCTGCTGCCGATGATCAGCCGTTGACGCTCGATGAGGTGGATGCGCTGCTGCGCTCAAGCACCC
TCCAAGCAGCCTAA

Upstream 100 bases:

>100_bases
CCATACTCAAGCAACTTAAACGCATCAGCCATATCCACCGCCCTGCGGCTGTTCGCCCTGAATTGTTCACCGGATGTTCT
TGCGGAAGGATTGTGCTTTC

Downstream 100 bases:

>100_bases
CGATTTTTCAAGCTGGCAATTGAAATTGCTATTTCAATGCCCTAGCTTGCTATACCTTGATGTGCCATTTTTCAGCTATT
TTTGGGAGTTTTCGTAATGG

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTRHFAIVDTTLREGEQWANAHFSSEDRLAIANLLVQFGVEYIEMTSPCASPQSTADLRSIAALPLGNTKLLTHTRCNLD
DVRLAAECGVAGVNILFGTSPYLRQWSHGRSIEAILEEVGPVVRFLQERNIEVRFSCEDSFRTPLPDLLRVYQAVDLLGV
QRVGIADTVGIATPRDVERVVGAVRNAVRCDIEFHGHNDGGCAIANAYAALEAGATHIDTTILGIGERNGIAALSGLIAR
LYLSEPESVAGYALPMLAQLDHTVAAILGIQVPFNACITSETAFAHKAGLHTKAVLANPSTYEAIDPKAFGRERNVLIGH
RLTGRHALQSRANTLGLDLSDTTIIAIAAQLKAAADDQPLTLDEVDALLRSSTLQAA

Sequences:

>Translated_377_residues
MTRHFAIVDTTLREGEQWANAHFSSEDRLAIANLLVQFGVEYIEMTSPCASPQSTADLRSIAALPLGNTKLLTHTRCNLD
DVRLAAECGVAGVNILFGTSPYLRQWSHGRSIEAILEEVGPVVRFLQERNIEVRFSCEDSFRTPLPDLLRVYQAVDLLGV
QRVGIADTVGIATPRDVERVVGAVRNAVRCDIEFHGHNDGGCAIANAYAALEAGATHIDTTILGIGERNGIAALSGLIAR
LYLSEPESVAGYALPMLAQLDHTVAAILGIQVPFNACITSETAFAHKAGLHTKAVLANPSTYEAIDPKAFGRERNVLIGH
RLTGRHALQSRANTLGLDLSDTTIIAIAAQLKAAADDQPLTLDEVDALLRSSTLQAA
>Mature_376_residues
TRHFAIVDTTLREGEQWANAHFSSEDRLAIANLLVQFGVEYIEMTSPCASPQSTADLRSIAALPLGNTKLLTHTRCNLDD
VRLAAECGVAGVNILFGTSPYLRQWSHGRSIEAILEEVGPVVRFLQERNIEVRFSCEDSFRTPLPDLLRVYQAVDLLGVQ
RVGIADTVGIATPRDVERVVGAVRNAVRCDIEFHGHNDGGCAIANAYAALEAGATHIDTTILGIGERNGIAALSGLIARL
YLSEPESVAGYALPMLAQLDHTVAAILGIQVPFNACITSETAFAHKAGLHTKAVLANPSTYEAIDPKAFGRERNVLIGHR
LTGRHALQSRANTLGLDLSDTTIIAIAAQLKAAADDQPLTLDEVDALLRSSTLQAA

Specific function: Catalyzes The Condensation Of The Acetyl Group Of Acetyl-CoA With 3-Methyl-2-Oxobutanoate (2-Oxoisovalerate) To Form 3-Carboxy-3-Hydroxy-4-Methylpentanoate (2-Isopropylmalate). [C]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=376, Percent_Identity=29.7872340425532, Blast_Score=152, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6320071, Length=369, Percent_Identity=49.8644986449864, Blast_Score=386, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6320019, Length=369, Percent_Identity=49.0514905149051, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011872
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 40487; Mature: 40356

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRHFAIVDTTLREGEQWANAHFSSEDRLAIANLLVQFGVEYIEMTSPCASPQSTADLRS
CCCEEEEEEHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHHHH
IAALPLGNTKLLTHTRCNLDDVRLAAECGVAGVNILFGTSPYLRQWSHGRSIEAILEEVG
HHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHH
PVVRFLQERNIEVRFSCEDSFRTPLPDLLRVYQAVDLLGVQRVGIADTVGIATPRDVERV
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHH
VGAVRNAVRCDIEFHGHNDGGCAIANAYAALEAGATHIDTTILGIGERNGIAALSGLIAR
HHHHHHHEEEEEEEECCCCCCEEHHHHHHHHHCCCCEECEEEEECCCCCCHHHHHHHHHH
LYLSEPESVAGYALPMLAQLDHTVAAILGIQVPFNACITSETAFAHKAGLHTKAVLANPS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHCCCCEEEEEECCC
TYEAIDPKAFGRERNVLIGHRLTGRHALQSRANTLGLDLSDTTIIAIAAQLKAAADDQPL
CCCCCCCHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCCCCC
TLDEVDALLRSSTLQAA
CHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRHFAIVDTTLREGEQWANAHFSSEDRLAIANLLVQFGVEYIEMTSPCASPQSTADLRS
CCEEEEEEHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCCHHHHHH
IAALPLGNTKLLTHTRCNLDDVRLAAECGVAGVNILFGTSPYLRQWSHGRSIEAILEEVG
HHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCHHHHHHHHHH
PVVRFLQERNIEVRFSCEDSFRTPLPDLLRVYQAVDLLGVQRVGIADTVGIATPRDVERV
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHH
VGAVRNAVRCDIEFHGHNDGGCAIANAYAALEAGATHIDTTILGIGERNGIAALSGLIAR
HHHHHHHEEEEEEEECCCCCCEEHHHHHHHHHCCCCEECEEEEECCCCCCHHHHHHHHHH
LYLSEPESVAGYALPMLAQLDHTVAAILGIQVPFNACITSETAFAHKAGLHTKAVLANPS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHCCCCEEEEEECCC
TYEAIDPKAFGRERNVLIGHRLTGRHALQSRANTLGLDLSDTTIIAIAAQLKAAADDQPL
CCCCCCCHHCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCCCCCC
TLDEVDALLRSSTLQAA
CHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9868782 [H]