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The map label for this gene is proB

Identifier: 159899724

GI number: 159899724

Start: 4055329

End: 4056441

Strand: Direct

Name: proB

Synonym: Haur_3206

Alternate gene names: 159899724

Gene position: 4055329-4056441 (Clockwise)

Preceding gene: 159899723

Following gene: 159899725

Centisome position: 63.9

GC content: 54.27

Gene sequence:

>1113_bases
ATGGATAGAGATCTGCATATGCGAATTGTAGTTAAACTTGGGACGAGCGTTTTGACCGATGGAACCGACCGTTTACATCG
CCCGCGAATGGTCGATTTGGCGCGTCAGATGGCCCAATTACGTGAGGCTGGACACGAAGTTGTCTTAGTTTCTTCAGGCG
CAGTTTTGGCTGGCTGGGAGCGCTTGGGCTTTCCCAAACGGCGGCGTGAGTTGACCCATAAACAGGCTTTGGCGGCAGTT
GGGCAAGGTCGTTTGATGCATATTTATGGCCAATTATTTGAAATTTACGATGTGCCGGTGGCTCAAACGCTGCTAACCCG
CGCCGACCTGCGTGATCGAGTGCGCTACCTGAATGCGCGTGCCACCTTATTAACCTGCTTGGAAATTGGCGTATTGCCAA
TTATCAATGAAAATGATGCAGTGGCGGTTGATGAAATTCGGGTCGGCGATAACGACACACTTTCAGCCTTGGTTGCCAAT
TTGGTTGATGCCCAGTTGTTGGTTATTTTGAGCGATATTGCTGGCTTGTATAGCGCCGACCCGCGCCATAACCCCGAAGC
AACCCTCATCGACGACGTTGCAGCAGTCAATGAACAGGTTTATGCTTTGGCAGGAGCGGCGGGCTCGCATCGTGGCACTG
GTGGCATGTTCACCAAAATTCAGGCTGCCGAATTGGCAACTCGCGCTGGCACAACTATGGTGATTGCTGCTGGCAACGAG
CCGAATGTGCTGGTGCGCCTGGTTGCTGGCGAGAGTGTAGGCACCCGCTTTCGGCCAGTCAGCACCCGCTTGGAGAGCCG
CAAACGCTGGATTATGGCTGAGCGCGTGCGCCAAGCCCATATTGCAGTTGATGCTGGTGCCGTGCAAGCCCTCACTCAGC
AAGGCCGTAGCCTGTTGCCCGCTGGAATTGTGGCAGTTGAAGGCGAATGGCGACGGGGCCAAACCGTGGCGATTGTCGCA
CCCGACGGCCAAACGATCGCCTGTGGCTTATGCCAATATGCCGCCCACGAAGTTGCCCAAATCAAAGGATCACGTACCAG
CGACATCGAAAGCATTCTCGGCTATTCCTACGGCGCTGAAGTCATTCACCGCGATGATATGGTAGCCTTTTAA

Upstream 100 bases:

>100_bases
TGCTCTTCGTGCTCTTCGTGGTTTCAAAATGCAGATTTTATGGTTTGGTTAAAATCAATTTAGCGCCTCTGCGTTAAAAT
AGCTATTTGTAATAATCTTG

Downstream 100 bases:

>100_bases
AGTCAAAGGGCAGAAGGCAAAAGTCAAAATCTTGAAATTTAGCTTTATGTTGCCTTTAATCTGGATTTATGCTTAATATC
AAAGGATAGCAGATAGAACT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MDRDLHMRIVVKLGTSVLTDGTDRLHRPRMVDLARQMAQLREAGHEVVLVSSGAVLAGWERLGFPKRRRELTHKQALAAV
GQGRLMHIYGQLFEIYDVPVAQTLLTRADLRDRVRYLNARATLLTCLEIGVLPIINENDAVAVDEIRVGDNDTLSALVAN
LVDAQLLVILSDIAGLYSADPRHNPEATLIDDVAAVNEQVYALAGAAGSHRGTGGMFTKIQAAELATRAGTTMVIAAGNE
PNVLVRLVAGESVGTRFRPVSTRLESRKRWIMAERVRQAHIAVDAGAVQALTQQGRSLLPAGIVAVEGEWRRGQTVAIVA
PDGQTIACGLCQYAAHEVAQIKGSRTSDIESILGYSYGAEVIHRDDMVAF

Sequences:

>Translated_370_residues
MDRDLHMRIVVKLGTSVLTDGTDRLHRPRMVDLARQMAQLREAGHEVVLVSSGAVLAGWERLGFPKRRRELTHKQALAAV
GQGRLMHIYGQLFEIYDVPVAQTLLTRADLRDRVRYLNARATLLTCLEIGVLPIINENDAVAVDEIRVGDNDTLSALVAN
LVDAQLLVILSDIAGLYSADPRHNPEATLIDDVAAVNEQVYALAGAAGSHRGTGGMFTKIQAAELATRAGTTMVIAAGNE
PNVLVRLVAGESVGTRFRPVSTRLESRKRWIMAERVRQAHIAVDAGAVQALTQQGRSLLPAGIVAVEGEWRRGQTVAIVA
PDGQTIACGLCQYAAHEVAQIKGSRTSDIESILGYSYGAEVIHRDDMVAF
>Mature_370_residues
MDRDLHMRIVVKLGTSVLTDGTDRLHRPRMVDLARQMAQLREAGHEVVLVSSGAVLAGWERLGFPKRRRELTHKQALAAV
GQGRLMHIYGQLFEIYDVPVAQTLLTRADLRDRVRYLNARATLLTCLEIGVLPIINENDAVAVDEIRVGDNDTLSALVAN
LVDAQLLVILSDIAGLYSADPRHNPEATLIDDVAAVNEQVYALAGAAGSHRGTGGMFTKIQAAELATRAGTTMVIAAGNE
PNVLVRLVAGESVGTRFRPVSTRLESRKRWIMAERVRQAHIAVDAGAVQALTQQGRSLLPAGIVAVEGEWRRGQTVAIVA
PDGQTIACGLCQYAAHEVAQIKGSRTSDIESILGYSYGAEVIHRDDMVAF

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=288, Percent_Identity=34.7222222222222, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=34.6153846153846, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=50.8333333333333, Blast_Score=347, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17569967, Length=271, Percent_Identity=35.7933579335793, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17569969, Length=271, Percent_Identity=35.7933579335793, Blast_Score=144, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=414, Percent_Identity=35.024154589372, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=32.2966507177034, Blast_Score=208, Evalue=9e-55,
Organism=Drosophila melanogaster, GI21357643, Length=322, Percent_Identity=31.6770186335404, Blast_Score=131, Evalue=9e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_HERA2 (A9B6X5)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001545971.1
- ProteinModelPortal:   A9B6X5
- SMR:   A9B6X5
- GeneID:   5735074
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_3206
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   HAUR316274:HAUR_3206-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40088; Mature: 40088

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRDLHMRIVVKLGTSVLTDGTDRLHRPRMVDLARQMAQLREAGHEVVLVSSGAVLAGWE
CCCCEEEEEEEECCCHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEECHH
RLGFPKRRRELTHKQALAAVGQGRLMHIYGQLFEIYDVPVAQTLLTRADLRDRVRYLNAR
HHCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ATLLTCLEIGVLPIINENDAVAVDEIRVGDNDTLSALVANLVDAQLLVILSDIAGLYSAD
HHHHHHHHHCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PRHNPEATLIDDVAAVNEQVYALAGAAGSHRGTGGMFTKIQAAELATRAGTTMVIAAGNE
CCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEHHHHHHHHCCCEEEEEECCC
PNVLVRLVAGESVGTRFRPVSTRLESRKRWIMAERVRQAHIAVDAGAVQALTQQGRSLLP
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCCCCC
AGIVAVEGEWRRGQTVAIVAPDGQTIACGLCQYAAHEVAQIKGSRTSDIESILGYSYGAE
CCEEEECCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCH
VIHRDDMVAF
HEECCCCCCC
>Mature Secondary Structure
MDRDLHMRIVVKLGTSVLTDGTDRLHRPRMVDLARQMAQLREAGHEVVLVSSGAVLAGWE
CCCCEEEEEEEECCCHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEECHH
RLGFPKRRRELTHKQALAAVGQGRLMHIYGQLFEIYDVPVAQTLLTRADLRDRVRYLNAR
HHCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ATLLTCLEIGVLPIINENDAVAVDEIRVGDNDTLSALVANLVDAQLLVILSDIAGLYSAD
HHHHHHHHHCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PRHNPEATLIDDVAAVNEQVYALAGAAGSHRGTGGMFTKIQAAELATRAGTTMVIAAGNE
CCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEHHHHHHHHCCCEEEEEECCC
PNVLVRLVAGESVGTRFRPVSTRLESRKRWIMAERVRQAHIAVDAGAVQALTQQGRSLLP
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCCCCC
AGIVAVEGEWRRGQTVAIVAPDGQTIACGLCQYAAHEVAQIKGSRTSDIESILGYSYGAE
CCEEEECCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCH
VIHRDDMVAF
HEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA