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The map label for this gene is lgrD [H]

Identifier: 159899645

GI number: 159899645

Start: 3947286

End: 3949175

Strand: Direct

Name: lgrD [H]

Synonym: Haur_3127

Alternate gene names: 159899645

Gene position: 3947286-3949175 (Clockwise)

Preceding gene: 159899644

Following gene: 159899646

Centisome position: 62.2

GC content: 52.43

Gene sequence:

>1890_bases
ATGTCAATAGCGACATTAGCGCCGATCACCGCCGACCGCACAGGCACAGTTTTTGTGCATGATGTTATCAGCACGCACGC
CCAGCATTCGCCACAGGCTATTGCCATTGCTACCAGCACATTCAAGTTGAGTTACGCTGAATTCGAACAGCGCACCAATC
AGCTTGCCCATTATTTACACCGCCAAGGAGTGCATCGTGGCCACACAGTTGGCGCGTGCTTTGAGCGCTCAGTCGAGGCA
ATGATCGCCGCCGTGGCGATTTGGAAGGCTGGCGCAGTCTACTTGCCGCTTGATCCAGGCTATCCCCAAGAACGGCTTAA
ATATATGTTGGGCAATAGTGGCGCGAGTTTGGTACTGGCAACCCAACTCACCGCCAGCCAGTTTCCTGAGCAACAATTGC
ATATTTTTGAGCAATTAGCCGCTGAGCTTGCACAGCAACCAAGCCATGCCCCTGAACATCAACTTACACCCGACGATCTG
GCCTATATCATTTATACCTCTGGCTCAACTGGCAAGCCCAAAGGCGTGCTCGTGCCGCATCGCGGTTTGGCCAATTTGGC
GGCTGCTCAAACCGAGCGCTTTGGCATCAACAGCCAATCACGAATTTTGCAGTTTGCCTCGCCAAGCTTCGATGCTTCGA
TTTCTGAGATGCTGACGGCTTTTTTTCAGGCTACAACTTTGTTTGTAGCCCCAACGAACGATCTTTTGCCCGGCCCAGAC
TTATTGACAACCCTGCGTGATCACCACATCACGGTTGCCACCCTGCCGCCTTCGGTGCTGGCGTTGCTCGATCCACGCGA
CTTGCCCAATTTACAAACCATCGTTTCGGCAGGCGAGGCTTGCACCGCTGAAATTGTGGCCCGTTGGGGGACAAACCGCC
GTTTTATCAATGCCTATGGCCCGACCGAAGTCACCGTTTGCGCCACCATGAGCCAGTCTTTACGCTACGGTATGGCCGTC
AGCATTGGCAACGCTATCAGCAATAGCCAAACCTATATCGTTGATGAGCACTTAAATTTGGTCGAAGGCGAGGCGGTTGG
CGAATTATTGGTCAGCAGTGTTGGCTTAGCCCATGGCTACCTTGGCCTTGGCGATCAAACTGCCGAGCGCTTTTTGCCCA
ATCCATGGAGCGACCAAGCGGGCAGTCGGATGTATCGCACTGGCGATTTGGTGCGGCGCTTGAGCGATGGCAGCCTTGAG
TTTCGCGGACGCATTGATCATCAAATTAAGCATCGCGGCTATCGCATCGATCCAGGCGAAATTGAAATGCTCTTGATGGA
ATATCCTAATGTGCGCCACGCCGTCGTCACGCTGCATCACGACCATAACCAGACCGAGCGGTTGGTCTCGTATTTGGTGT
TACACGGCGAAGTTATGCCCTACTATCGCGATATTTACCGCTATTTGGAGAGCATGCTGCCCAAATATATGGTACCGCTC
TCGTATACGGTCGTGAAAGAATTGCCACGCACGCCCAATGGCAAACTCGATTTAGCAGCCTTGCCTGAGCCAGATTTTGC
CTTGCTGACCGTTAGTGAAAATTATGTTGCCCCACGCACACCGCTTGAACAGCAGATCGCCGCGATTTGGGAAAATATTT
TGGATACTCCCAATATTGGTGTGCTTGATGATTTCTTTGATGCTGGCGGCCACTCGCTGTTAGCAACCCAAATTGTTTCA
GCGATTCGCAGCACGTTTGCGGTGGAAATTCCACTTTCAGTCTTGCTTGGCGTTGAGCCAACCATCGCCGCCACGGCCCA
ATTGATCGAGCAATATCAAATTGCTAATGCCGATGATGCCGAACTCGCCGACCTGTTGAACGAACTTGATGGCCTCTCCG
ATGAAGAAATTCAAGCCTTGTTAGCTGATGAAGGAGCGCTCACCGCATGA

Upstream 100 bases:

>100_bases
CTACGGGGCTATTTGGCGGTTGCGCTGAGGCTATCGCTGCTCCATAGGCATCGCAACAATTCTAAAACTACAATCTAAAA
CGATGCGAAGGAGCCGTGTT

Downstream 100 bases:

>100_bases
ACTTTTTGCTAATTCAGCCGCTTGATTATTTGTTCGTCTCTGGCGGGGCGCATAAAGCCAATCGTTTTTTGATGGAAGGC
CTCGTCGAGCGCGGACACAA

Product: amino acid adenylation domain-containing protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent D-leucine adenylase; D-LeuA; D-Leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent glycine adenylase; GlyA; Glycine activase; Linear gramicidin--PCP reductase [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSIATLAPITADRTGTVFVHDVISTHAQHSPQAIAIATSTFKLSYAEFEQRTNQLAHYLHRQGVHRGHTVGACFERSVEA
MIAAVAIWKAGAVYLPLDPGYPQERLKYMLGNSGASLVLATQLTASQFPEQQLHIFEQLAAELAQQPSHAPEHQLTPDDL
AYIIYTSGSTGKPKGVLVPHRGLANLAAAQTERFGINSQSRILQFASPSFDASISEMLTAFFQATTLFVAPTNDLLPGPD
LLTTLRDHHITVATLPPSVLALLDPRDLPNLQTIVSAGEACTAEIVARWGTNRRFINAYGPTEVTVCATMSQSLRYGMAV
SIGNAISNSQTYIVDEHLNLVEGEAVGELLVSSVGLAHGYLGLGDQTAERFLPNPWSDQAGSRMYRTGDLVRRLSDGSLE
FRGRIDHQIKHRGYRIDPGEIEMLLMEYPNVRHAVVTLHHDHNQTERLVSYLVLHGEVMPYYRDIYRYLESMLPKYMVPL
SYTVVKELPRTPNGKLDLAALPEPDFALLTVSENYVAPRTPLEQQIAAIWENILDTPNIGVLDDFFDAGGHSLLATQIVS
AIRSTFAVEIPLSVLLGVEPTIAATAQLIEQYQIANADDAELADLLNELDGLSDEEIQALLADEGALTA

Sequences:

>Translated_629_residues
MSIATLAPITADRTGTVFVHDVISTHAQHSPQAIAIATSTFKLSYAEFEQRTNQLAHYLHRQGVHRGHTVGACFERSVEA
MIAAVAIWKAGAVYLPLDPGYPQERLKYMLGNSGASLVLATQLTASQFPEQQLHIFEQLAAELAQQPSHAPEHQLTPDDL
AYIIYTSGSTGKPKGVLVPHRGLANLAAAQTERFGINSQSRILQFASPSFDASISEMLTAFFQATTLFVAPTNDLLPGPD
LLTTLRDHHITVATLPPSVLALLDPRDLPNLQTIVSAGEACTAEIVARWGTNRRFINAYGPTEVTVCATMSQSLRYGMAV
SIGNAISNSQTYIVDEHLNLVEGEAVGELLVSSVGLAHGYLGLGDQTAERFLPNPWSDQAGSRMYRTGDLVRRLSDGSLE
FRGRIDHQIKHRGYRIDPGEIEMLLMEYPNVRHAVVTLHHDHNQTERLVSYLVLHGEVMPYYRDIYRYLESMLPKYMVPL
SYTVVKELPRTPNGKLDLAALPEPDFALLTVSENYVAPRTPLEQQIAAIWENILDTPNIGVLDDFFDAGGHSLLATQIVS
AIRSTFAVEIPLSVLLGVEPTIAATAQLIEQYQIANADDAELADLLNELDGLSDEEIQALLADEGALTA
>Mature_628_residues
SIATLAPITADRTGTVFVHDVISTHAQHSPQAIAIATSTFKLSYAEFEQRTNQLAHYLHRQGVHRGHTVGACFERSVEAM
IAAVAIWKAGAVYLPLDPGYPQERLKYMLGNSGASLVLATQLTASQFPEQQLHIFEQLAAELAQQPSHAPEHQLTPDDLA
YIIYTSGSTGKPKGVLVPHRGLANLAAAQTERFGINSQSRILQFASPSFDASISEMLTAFFQATTLFVAPTNDLLPGPDL
LTTLRDHHITVATLPPSVLALLDPRDLPNLQTIVSAGEACTAEIVARWGTNRRFINAYGPTEVTVCATMSQSLRYGMAVS
IGNAISNSQTYIVDEHLNLVEGEAVGELLVSSVGLAHGYLGLGDQTAERFLPNPWSDQAGSRMYRTGDLVRRLSDGSLEF
RGRIDHQIKHRGYRIDPGEIEMLLMEYPNVRHAVVTLHHDHNQTERLVSYLVLHGEVMPYYRDIYRYLESMLPKYMVPLS
YTVVKELPRTPNGKLDLAALPEPDFALLTVSENYVAPRTPLEQQIAAIWENILDTPNIGVLDDFFDAGGHSLLATQIVSA
IRSTFAVEIPLSVLLGVEPTIAATAQLIEQYQIANADDAELADLLNELDGLSDEEIQALLADEGALTA

Specific function: Activates the 13th to the 16th (Trp, D-Leu, Trp and Gly) amino acids in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 14th (D-Leu) amino acid. It also catalyzes

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=439, Percent_Identity=27.7904328018223, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI187761345, Length=558, Percent_Identity=23.4767025089606, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI187761343, Length=558, Percent_Identity=23.4767025089606, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI58082049, Length=505, Percent_Identity=23.3663366336634, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI157311624, Length=508, Percent_Identity=22.6377952755905, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311622, Length=508, Percent_Identity=22.6377952755905, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI38505220, Length=547, Percent_Identity=25.4113345521024, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI42544132, Length=509, Percent_Identity=24.75442043222, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI122937307, Length=504, Percent_Identity=23.4126984126984, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI115511026, Length=504, Percent_Identity=24.0079365079365, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1786801, Length=592, Percent_Identity=32.2635135135135, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI145693145, Length=528, Percent_Identity=23.2954545454545, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1788107, Length=513, Percent_Identity=23.0019493177388, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786810, Length=516, Percent_Identity=24.2248062015504, Blast_Score=76, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17556356, Length=556, Percent_Identity=27.158273381295, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17550940, Length=595, Percent_Identity=23.1932773109244, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=536, Percent_Identity=22.9477611940299, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=536, Percent_Identity=22.2014925373134, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=521, Percent_Identity=22.8406909788868, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=507, Percent_Identity=20.3155818540434, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17560308, Length=521, Percent_Identity=23.0326295585413, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32564422, Length=413, Percent_Identity=25.4237288135593, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI32564420, Length=416, Percent_Identity=25.2403846153846, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17560140, Length=524, Percent_Identity=21.3740458015267, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17559526, Length=307, Percent_Identity=27.0358306188925, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319591, Length=684, Percent_Identity=26.4619883040936, Blast_Score=204, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24648676, Length=498, Percent_Identity=29.5180722891566, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24581924, Length=529, Percent_Identity=20.9829867674858, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI281365686, Length=522, Percent_Identity=21.455938697318, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR013120
- InterPro:   IPR016040
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR010080 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF07993 NAD_binding_4; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 68856; Mature: 68725

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIATLAPITADRTGTVFVHDVISTHAQHSPQAIAIATSTFKLSYAEFEQRTNQLAHYLH
CCCCEECCCCCCCCCCEEEHHHHHHHCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHH
RQGVHRGHTVGACFERSVEAMIAAVAIWKAGAVYLPLDPGYPQERLKYMLGNSGASLVLA
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEE
TQLTASQFPEQQLHIFEQLAAELAQQPSHAPEHQLTPDDLAYIIYTSGSTGKPKGVLVPH
EEHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECC
RGLANLAAAQTERFGINSQSRILQFASPSFDASISEMLTAFFQATTLFVAPTNDLLPGPD
CCHHHHHHHHHHHCCCCCHHHHEEECCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCH
LLTTLRDHHITVATLPPSVLALLDPRDLPNLQTIVSAGEACTAEIVARWGTNRRFINAYG
HHHHHHHCCEEEEECCHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCEEEEECC
PTEVTVCATMSQSLRYGMAVSIGNAISNSQTYIVDEHLNLVEGEAVGELLVSSVGLAHGY
CCEEEEEHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
LGLGDQTAERFLPNPWSDQAGSRMYRTGDLVRRLSDGSLEFRGRIDHQIKHRGYRIDPGE
CCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHCCEECCCHH
IEMLLMEYPNVRHAVVTLHHDHNQTERLVSYLVLHGEVMPYYRDIYRYLESMLPKYMVPL
EEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
SYTVVKELPRTPNGKLDLAALPEPDFALLTVSENYVAPRTPLEQQIAAIWENILDTPNIG
HHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCC
VLDDFFDAGGHSLLATQIVSAIRSTFAVEIPLSVLLGVEPTIAATAQLIEQYQIANADDA
CHHHHHHCCCCHHHHHHHHHHHHHHEEEEECHHHHHCCCCHHHHHHHHHHHHHCCCCCHH
ELADLLNELDGLSDEEIQALLADEGALTA
HHHHHHHHHCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SIATLAPITADRTGTVFVHDVISTHAQHSPQAIAIATSTFKLSYAEFEQRTNQLAHYLH
CCCEECCCCCCCCCCEEEHHHHHHHCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHH
RQGVHRGHTVGACFERSVEAMIAAVAIWKAGAVYLPLDPGYPQERLKYMLGNSGASLVLA
HCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEE
TQLTASQFPEQQLHIFEQLAAELAQQPSHAPEHQLTPDDLAYIIYTSGSTGKPKGVLVPH
EEHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECC
RGLANLAAAQTERFGINSQSRILQFASPSFDASISEMLTAFFQATTLFVAPTNDLLPGPD
CCHHHHHHHHHHHCCCCCHHHHEEECCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCH
LLTTLRDHHITVATLPPSVLALLDPRDLPNLQTIVSAGEACTAEIVARWGTNRRFINAYG
HHHHHHHCCEEEEECCHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCEEEEECC
PTEVTVCATMSQSLRYGMAVSIGNAISNSQTYIVDEHLNLVEGEAVGELLVSSVGLAHGY
CCEEEEEHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
LGLGDQTAERFLPNPWSDQAGSRMYRTGDLVRRLSDGSLEFRGRIDHQIKHRGYRIDPGE
CCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHCCEECCCHH
IEMLLMEYPNVRHAVVTLHHDHNQTERLVSYLVLHGEVMPYYRDIYRYLESMLPKYMVPL
EEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
SYTVVKELPRTPNGKLDLAALPEPDFALLTVSENYVAPRTPLEQQIAAIWENILDTPNIG
HHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCC
VLDDFFDAGGHSLLATQIVSAIRSTFAVEIPLSVLLGVEPTIAATAQLIEQYQIANADDA
CHHHHHHCCCCHHHHHHHHHHHHHHEEEEECHHHHHCCCCHHHHHHHHHHHHHCCCCCHH
ELADLLNELDGLSDEEIQALLADEGALTA
HHHHHHHHHCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA