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The map label for this gene is comEC [H]

Identifier: 159899290

GI number: 159899290

Start: 3528808

End: 3529911

Strand: Direct

Name: comEC [H]

Synonym: Haur_2771

Alternate gene names: 159899290

Gene position: 3528808-3529911 (Clockwise)

Preceding gene: 159899289

Following gene: 159899291

Centisome position: 55.6

GC content: 46.2

Gene sequence:

>1104_bases
ATGACAGCACCATCTTTCCCAGCTTTTTGCAATCGTTGTGGCAACCGGCGGAGTCCTCAAGCGCGGTTTTGCAGATACTG
TGGTAATGCCTTTCAGCATTGGCAGGCCACAGCAACCAAGTCGATCCCTTCAACTGAGGTAGGCGTAAAGGCTGTGCAGC
GTAGCATAAAATGGCGTTATTGGTTTGCGTTAACGCTTGTTTTATTCATTGGTGCACGACTTAGCCTAAAACCATTGATG
GGATTGGTACTAGGTGGTTTAAATTTTAGCAACGCACCAATTGCTGGTACTCCTAACCAGAGTCCGCCTAATCAACGCCT
TGCCACAGGCCAAACGCTCCGAATTCACGTGATCGATGTTGATCAGGGGGACAGTATCTTAATTCAAACTCCACAGGGGA
TGAACGCCTTAATTGACGGCGGCTATGACAATGGTTTGGCATTAGAATACTTACACCAACAAGGCATTCAACGAATCGAT
CATATGATCGTATCACACCCTCATGCTGATCATATTGGCGGGCTGGTTGAGGTGCTGAATTCTCTCGAAGTTGGCAGTAT
TTGGACATCGGGTGCAGTTCACTCCACCAGTTATTTTGAGAATTTGCTTGATGCGATTGATCGTCAACAAGTGCCATATC
GCGAAGTTAAAACCCAAGAGTCTATTCCTCTGGGCGATTTGAGTTTATTAGTTGTCCGCAGCGAACCACAGGCTGGTGAT
TTAAATGATACCTCCCTGATTTTGCGTTTAGAGTATGGTCAATTTAGTATGCTTTTTACTGGTGATGCTGAAGCGAGTAG
CGAGGTCGATGTTTTACAAACAACGCCCAATTTGTTACCAGCTACGGTGCTTAAGGTTGGCCATCATGGGTCATATACCT
CAACGACTCCTGCATTTGCCCTAGCGGTTGATCCAACTCTAGCGATCTATTCAGCAGGTCGTAATAATTCTTATGGCCAT
CCACATCAACAAACATTAACAACATTGTGCAATCTCGGGGCAACGGTTTATGGCACTGATCGTCATGGGACAGTCGTTAT
AACCTCCGATGGACTTGAGTATCAGGTGCAAACGGCCCAGCGAACAACAGCACTGTGTCATTAA

Upstream 100 bases:

>100_bases
GCTCTTATCGTTCATAGCAATTCAATACAGCAATTATGGTAATAAACTGCGTTATAATGAAGCTAGTACCTAGAATTTTA
GCGTATGGCTAGATTTGCCT

Downstream 100 bases:

>100_bases
GGAGCCAATAATGCTTGTAGTTTTTGATCGCTATGAAGGTGAGCATGCGGTATTAATTGATCAGCATAATCACCAAATCT
ATAATATTGTCATCGGTTGC

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MTAPSFPAFCNRCGNRRSPQARFCRYCGNAFQHWQATATKSIPSTEVGVKAVQRSIKWRYWFALTLVLFIGARLSLKPLM
GLVLGGLNFSNAPIAGTPNQSPPNQRLATGQTLRIHVIDVDQGDSILIQTPQGMNALIDGGYDNGLALEYLHQQGIQRID
HMIVSHPHADHIGGLVEVLNSLEVGSIWTSGAVHSTSYFENLLDAIDRQQVPYREVKTQESIPLGDLSLLVVRSEPQAGD
LNDTSLILRLEYGQFSMLFTGDAEASSEVDVLQTTPNLLPATVLKVGHHGSYTSTTPAFALAVDPTLAIYSAGRNNSYGH
PHQQTLTTLCNLGATVYGTDRHGTVVITSDGLEYQVQTAQRTTALCH

Sequences:

>Translated_367_residues
MTAPSFPAFCNRCGNRRSPQARFCRYCGNAFQHWQATATKSIPSTEVGVKAVQRSIKWRYWFALTLVLFIGARLSLKPLM
GLVLGGLNFSNAPIAGTPNQSPPNQRLATGQTLRIHVIDVDQGDSILIQTPQGMNALIDGGYDNGLALEYLHQQGIQRID
HMIVSHPHADHIGGLVEVLNSLEVGSIWTSGAVHSTSYFENLLDAIDRQQVPYREVKTQESIPLGDLSLLVVRSEPQAGD
LNDTSLILRLEYGQFSMLFTGDAEASSEVDVLQTTPNLLPATVLKVGHHGSYTSTTPAFALAVDPTLAIYSAGRNNSYGH
PHQQTLTTLCNLGATVYGTDRHGTVVITSDGLEYQVQTAQRTTALCH
>Mature_366_residues
TAPSFPAFCNRCGNRRSPQARFCRYCGNAFQHWQATATKSIPSTEVGVKAVQRSIKWRYWFALTLVLFIGARLSLKPLMG
LVLGGLNFSNAPIAGTPNQSPPNQRLATGQTLRIHVIDVDQGDSILIQTPQGMNALIDGGYDNGLALEYLHQQGIQRIDH
MIVSHPHADHIGGLVEVLNSLEVGSIWTSGAVHSTSYFENLLDAIDRQQVPYREVKTQESIPLGDLSLLVVRSEPQAGDL
NDTSLILRLEYGQFSMLFTGDAEASSEVDVLQTTPNLLPATVLKVGHHGSYTSTTPAFALAVDPTLAIYSAGRNNSYGHP
HQQTLTTLCNLGATVYGTDRHGTVVITSDGLEYQVQTAQRTTALCH

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=263, Percent_Identity=26.9961977186312, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 39951; Mature: 39819

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPSFPAFCNRCGNRRSPQARFCRYCGNAFQHWQATATKSIPSTEVGVKAVQRSIKWRY
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
WFALTLVLFIGARLSLKPLMGLVLGGLNFSNAPIAGTPNQSPPNQRLATGQTLRIHVIDV
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEE
DQGDSILIQTPQGMNALIDGGYDNGLALEYLHQQGIQRIDHMIVSHPHADHIGGLVEVLN
CCCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SLEVGSIWTSGAVHSTSYFENLLDAIDRQQVPYREVKTQESIPLGDLSLLVVRSEPQAGD
CCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEECCCCCCC
LNDTSLILRLEYGQFSMLFTGDAEASSEVDVLQTTPNLLPATVLKVGHHGSYTSTTPAFA
CCCCEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCEEE
LAVDPTLAIYSAGRNNSYGHPHQQTLTTLCNLGATVYGTDRHGTVVITSDGLEYQVQTAQ
EEECCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEECCCCEEEEEHHH
RTTALCH
HHHHCCC
>Mature Secondary Structure 
TAPSFPAFCNRCGNRRSPQARFCRYCGNAFQHWQATATKSIPSTEVGVKAVQRSIKWRY
CCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
WFALTLVLFIGARLSLKPLMGLVLGGLNFSNAPIAGTPNQSPPNQRLATGQTLRIHVIDV
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEE
DQGDSILIQTPQGMNALIDGGYDNGLALEYLHQQGIQRIDHMIVSHPHADHIGGLVEVLN
CCCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SLEVGSIWTSGAVHSTSYFENLLDAIDRQQVPYREVKTQESIPLGDLSLLVVRSEPQAGD
CCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEECCCCCCC
LNDTSLILRLEYGQFSMLFTGDAEASSEVDVLQTTPNLLPATVLKVGHHGSYTSTTPAFA
CCCCEEEEEEECCCEEEEEECCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCEEE
LAVDPTLAIYSAGRNNSYGHPHQQTLTTLCNLGATVYGTDRHGTVVITSDGLEYQVQTAQ
EEECCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCEEEEECCCCEEEEEHHH
RTTALCH
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]