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The map label for this gene is phaZ1 [H]

Identifier: 159899289

GI number: 159899289

Start: 3527026

End: 3528660

Strand: Direct

Name: phaZ1 [H]

Synonym: Haur_2770

Alternate gene names: 159899289

Gene position: 3527026-3528660 (Clockwise)

Preceding gene: 159899288

Following gene: 159899290

Centisome position: 55.57

GC content: 50.89

Gene sequence:

>1635_bases
ATGAAACGAGGATGGCTGATCGTACTGCTGTTACTTGGCATTTTGGCAAGTTTTTGGAGTCCACAGGCGGCGAGTGCAGG
TCAATGGATTACCGGTTCAGTTACAACGAGCGCCGGTTCGCGCAATTACAAACTATGGGTTCCAACTAATTATTCGACCT
CAACGCCTACCGCCTTAGTGGTGCTGTTACATGGCTGTACTCAAACGCCCGATGATTTTGCCCGTGGCACCGAAATGAAC
AATTTGGCCGATAACCAAACGTTTTTGGTACTCTACCCTGAACAACCAACCAGCAGTAATGCCAATCGTTGCTGGCAATG
GTGGGATAGCCAACATCAAACACGGGGGGCAGGCGAACCCAGCATTATCGCCGCAATGGTGACCAGCATTCAAGCGAGCT
ATAACGTTGATCCAAGTCGGCGTTATGTTGCAGGGATTTCGGCTGGTGCAGCGATGAGCGTCATTATGGGCGCGACCTAT
CCCGATATCTTTTCAGCAATTGGGGTTGTTGGTGGCTTGGAATACAAGGCCGCAACCAGCGTGTTGAATGTTTCGACGGC
CATGCAATCGGGTGGCCCCAACCCCGAAGCTCAAGGCTTGGCCGCCTATCAATCGATCGGTACGCGAGCCAATTTGGTGC
GGGTGATGGTGATTCATGGAACGGCGGACAGCGTGGTTACGCCGATCAACGGCCAACAAGTTGTCCAGCAGTGGCTCACC
ACCAACGATTATCTTGATGATACTCAGCGCAATTATTCAGTCGATGCAACCGTTGATCAAACAATTTCGGGTACTGTGCC
TAACGGTCGGAGCTACACCCGCACTATCTACAACAATGCAACCAACCAACCAATTATTGAACATTGGGCAATTAATGGGA
TGGGGCATGCATGGTCAGGTGGCAGCAGTTCAGGTTCCTACACCGATCCTCAAGGCCCAAAAGCCAGCAATGAATTTTGG
CGGTTCTTTAGCCAAACCTCGGCCAGCCCCACCCCAACAACTACGCCAGCAGCCAGTGCCACCAGCACTCCACCCAGCAT
AACGCCAACAAATCCAACCTCGACCCCGATTGCTCCTAGCCCTACACCCAGTGCAGGCCAAAGCATGCAATTCCCTTCGT
GGGGCTTGGAAGATGGCTTGGTTGCCCAAACAACGTTTGGTGGATATGTATTGATTCCAAGTATGTATGTTGGTGATAGT
GGCAGCACCAGTCTGCGCGGAATCCTCTCATTTGACACCTCGGCCATTCCCGATGGGGCGACGATTACCAGCGTCAACCT
AAGCTTAGCGTATGAACAAAGCGCGATTGGCACTCCGTGGACAGGTCTTGGAAGCTTAGTTGCAGATCTAAATCAAGGTT
GTTTAAGTCTGACCTGTTATCTTGAATTGAGTGATTTTGGCACAACGACCACAGCCAGCAGTGCCATGAGCTTTCAGCAA
ACCCCCAATGGTTTGAATGGTAGCGCCAATTCAACCGGATTAAGTGCTATCAATAAACAAGGCCGTACCCAAATTCGGCT
GCGCTTTGAGAACTTAACCAATGCTAACAGCCAAGCTGATTATGTGCGGATTGCTGGTGGCGAAGCAATGTTGACAAGCA
ATCGCCCAGTCTTGACTGTAACCTATCAACCATAA

Upstream 100 bases:

>100_bases
GCGTGTGTAACTCAAGTGTAACTGCTTGCTGCTATGCTTAAAATCCTTCCTGCCCCATTGCCCACGTCGCCCAACTAGGT
TCAATCAAAGGAGATTGCTC

Downstream 100 bases:

>100_bases
TCCAAAGTTTATTGGATAAGAGCCATCCATCGCAGTTCAGATGGATGGCTCTTATCGTTCATAGCAATTCAATACAGCAA
TTATGGTAATAAACTGCGTT

Product: PHB depolymerase family esterase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MKRGWLIVLLLLGILASFWSPQAASAGQWITGSVTTSAGSRNYKLWVPTNYSTSTPTALVVLLHGCTQTPDDFARGTEMN
NLADNQTFLVLYPEQPTSSNANRCWQWWDSQHQTRGAGEPSIIAAMVTSIQASYNVDPSRRYVAGISAGAAMSVIMGATY
PDIFSAIGVVGGLEYKAATSVLNVSTAMQSGGPNPEAQGLAAYQSIGTRANLVRVMVIHGTADSVVTPINGQQVVQQWLT
TNDYLDDTQRNYSVDATVDQTISGTVPNGRSYTRTIYNNATNQPIIEHWAINGMGHAWSGGSSSGSYTDPQGPKASNEFW
RFFSQTSASPTPTTTPAASATSTPPSITPTNPTSTPIAPSPTPSAGQSMQFPSWGLEDGLVAQTTFGGYVLIPSMYVGDS
GSTSLRGILSFDTSAIPDGATITSVNLSLAYEQSAIGTPWTGLGSLVADLNQGCLSLTCYLELSDFGTTTTASSAMSFQQ
TPNGLNGSANSTGLSAINKQGRTQIRLRFENLTNANSQADYVRIAGGEAMLTSNRPVLTVTYQP

Sequences:

>Translated_544_residues
MKRGWLIVLLLLGILASFWSPQAASAGQWITGSVTTSAGSRNYKLWVPTNYSTSTPTALVVLLHGCTQTPDDFARGTEMN
NLADNQTFLVLYPEQPTSSNANRCWQWWDSQHQTRGAGEPSIIAAMVTSIQASYNVDPSRRYVAGISAGAAMSVIMGATY
PDIFSAIGVVGGLEYKAATSVLNVSTAMQSGGPNPEAQGLAAYQSIGTRANLVRVMVIHGTADSVVTPINGQQVVQQWLT
TNDYLDDTQRNYSVDATVDQTISGTVPNGRSYTRTIYNNATNQPIIEHWAINGMGHAWSGGSSSGSYTDPQGPKASNEFW
RFFSQTSASPTPTTTPAASATSTPPSITPTNPTSTPIAPSPTPSAGQSMQFPSWGLEDGLVAQTTFGGYVLIPSMYVGDS
GSTSLRGILSFDTSAIPDGATITSVNLSLAYEQSAIGTPWTGLGSLVADLNQGCLSLTCYLELSDFGTTTTASSAMSFQQ
TPNGLNGSANSTGLSAINKQGRTQIRLRFENLTNANSQADYVRIAGGEAMLTSNRPVLTVTYQP
>Mature_544_residues
MKRGWLIVLLLLGILASFWSPQAASAGQWITGSVTTSAGSRNYKLWVPTNYSTSTPTALVVLLHGCTQTPDDFARGTEMN
NLADNQTFLVLYPEQPTSSNANRCWQWWDSQHQTRGAGEPSIIAAMVTSIQASYNVDPSRRYVAGISAGAAMSVIMGATY
PDIFSAIGVVGGLEYKAATSVLNVSTAMQSGGPNPEAQGLAAYQSIGTRANLVRVMVIHGTADSVVTPINGQQVVQQWLT
TNDYLDDTQRNYSVDATVDQTISGTVPNGRSYTRTIYNNATNQPIIEHWAINGMGHAWSGGSSSGSYTDPQGPKASNEFW
RFFSQTSASPTPTTTPAASATSTPPSITPTNPTSTPIAPSPTPSAGQSMQFPSWGLEDGLVAQTTFGGYVLIPSMYVGDS
GSTSLRGILSFDTSAIPDGATITSVNLSLAYEQSAIGTPWTGLGSLVADLNQGCLSLTCYLELSDFGTTTTASSAMSFQQ
TPNGLNGSANSTGLSAINKQGRTQIRLRFENLTNANSQADYVRIAGGEAMLTSNRPVLTVTYQP

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 57524; Mature: 57524

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRGWLIVLLLLGILASFWSPQAASAGQWITGSVTTSAGSRNYKLWVPTNYSTSTPTALV
CCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCEEE
VLLHGCTQTPDDFARGTEMNNLADNQTFLVLYPEQPTSSNANRCWQWWDSQHQTRGAGEP
EEECCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCC
SIIAAMVTSIQASYNVDPSRRYVAGISAGAAMSVIMGATYPDIFSAIGVVGGLEYKAATS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
VLNVSTAMQSGGPNPEAQGLAAYQSIGTRANLVRVMVIHGTADSVVTPINGQQVVQQWLT
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCEECCCCHHHHHHHHHH
TNDYLDDTQRNYSVDATVDQTISGTVPNGRSYTRTIYNNATNQPIIEHWAINGMGHAWSG
CCCCCCCCCCCEEEECEECCCCCCCCCCCCCEEEEEECCCCCCCCHHHEEECCCCCCCCC
GSSSGSYTDPQGPKASNEFWRFFSQTSASPTPTTTPAASATSTPPSITPTNPTSTPIAPS
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PTPSAGQSMQFPSWGLEDGLVAQTTFGGYVLIPSMYVGDSGSTSLRGILSFDTSAIPDGA
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECEEEECCCCCCCEEEEEECCCCCCCCCC
TITSVNLSLAYEQSAIGTPWTGLGSLVADLNQGCLSLTCYLELSDFGTTTTASSAMSFQQ
EEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHC
TPNGLNGSANSTGLSAINKQGRTQIRLRFENLTNANSQADYVRIAGGEAMLTSNRPVLTV
CCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEECCCEEEECCCCEEEE
TYQP
EECC
>Mature Secondary Structure
MKRGWLIVLLLLGILASFWSPQAASAGQWITGSVTTSAGSRNYKLWVPTNYSTSTPTALV
CCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCEEE
VLLHGCTQTPDDFARGTEMNNLADNQTFLVLYPEQPTSSNANRCWQWWDSQHQTRGAGEP
EEECCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCC
SIIAAMVTSIQASYNVDPSRRYVAGISAGAAMSVIMGATYPDIFSAIGVVGGLEYKAATS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
VLNVSTAMQSGGPNPEAQGLAAYQSIGTRANLVRVMVIHGTADSVVTPINGQQVVQQWLT
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCEECCCCHHHHHHHHHH
TNDYLDDTQRNYSVDATVDQTISGTVPNGRSYTRTIYNNATNQPIIEHWAINGMGHAWSG
CCCCCCCCCCCEEEECEECCCCCCCCCCCCCEEEEEECCCCCCCCHHHEEECCCCCCCCC
GSSSGSYTDPQGPKASNEFWRFFSQTSASPTPTTTPAASATSTPPSITPTNPTSTPIAPS
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PTPSAGQSMQFPSWGLEDGLVAQTTFGGYVLIPSMYVGDSGSTSLRGILSFDTSAIPDGA
CCCCCCCCCCCCCCCCCCCEEEEEECCCEEEECEEEECCCCCCCEEEEEECCCCCCCCCC
TITSVNLSLAYEQSAIGTPWTGLGSLVADLNQGCLSLTCYLELSDFGTTTTASSAMSFQQ
EEEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHC
TPNGLNGSANSTGLSAINKQGRTQIRLRFENLTNANSQADYVRIAGGEAMLTSNRPVLTV
CCCCCCCCCCCCCHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEECCCEEEECCCCEEEE
TYQP
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]