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The map label for this gene is nirA [H]

Identifier: 159898270

GI number: 159898270

Start: 2032546

End: 2034207

Strand: Reverse

Name: nirA [H]

Synonym: Haur_1746

Alternate gene names: 159898270

Gene position: 2034207-2032546 (Counterclockwise)

Preceding gene: 159898271

Following gene: 159898269

Centisome position: 32.05

GC content: 51.74

Gene sequence:

>1662_bases
ATGACTGCTACAACCCCAAAACTAAACCCTGTCGAACTGCAAAAGCTGGAAAAAGATGGCTTAGCGGTGCTTGAAGATAT
TTATCGCTACGCCCAAAGTGGCGATATCAATGAAGTTACCGAAAGTGATATGAACCGTTTCAAATGGTATGGTTTGTATC
ACGATAAGCCCAAAGATGGCTTTTTCATGTTGCGGGTGCGCTTGCCTGGCGGGATTATGACTGCCGATCAAGCTGATGCA
ATTGTGCACTTGGCTGAAACCGTCGCCCCTGGTCGGGTTGAAATTACCACTCGCCAAACCTTCCAGTTGCATACAATTGC
GCTGCGCGATATTCCAACCGTATTTGAAACTTTGGAAAGCGTTGGGCTTTCGTCGATTGAGGCTTGTGGTGATGTGCCAC
GCAACGTTGTGAGTAGCCCAGTTGCTGGGATTGCCGCCGACGAATGGATCGATCCACGGCCATTCTTCAAGGCACTCGAT
GAGCACTTTGCCTATAACACCGATTACTCAAACTTGCCCCGCAAGTATAAAGTTTCGGTCGCTGGCGGCGGGCTTGACGC
GACCCAAGCACCAATCAATGATCTTTCGTTCACTCCTGGCCGCAAGGAAATTGATGGCGAGATTGTGCTGGGCTACAACG
TTTGGGTTGGGGGCGGGCTTTCGCACGAGCCACATCTAGCTCAAAACATTGATGTGTTTGTGCCAGCCGATCTCGATGCA
GTGGTTGAAATTGCTCGTGCCATCACCTTGGTCTATCGCGATTTTGGCTACCGCGAAAAGCGTAACCATGCCCGGCTAAA
ATATCTGATCGCCGATTGGGGTGCAGAGCGTTTCCGCGAAGAAGTTGTGAACTACCTTGGCCATCCCTTGGAACGAGCGG
CAACCGATGTGCCACCAGCAGTTTACAGTGGCGATGTCGTTGGAGTATTTCGCCAAAAGCAGCCAAATCTCTACTGGGTT
GGCTTGCTTGTACCAACTGGACGCATTACGCCAGCCCAAATTCGCGAAGCAGCTCGCTTATCACGTGAATATGGCCAAAG
CGAAATTCGCCTAACCCACAGCCAAAACTTGCTGTTGCCCTACATTCCTGAAGCGCGGTTAGATGCCTTACTGGCTGAGC
CGTTGTTGCAAGAATTGCAGCCAAATGGCCCACGTATCCAACGCACAGTCGTTGCTTGTACTGGGTTGCCCTACTGCAAC
TTTGCTACAATCGAAACCAAAGAAGCTGCTTGGCAATTGGCTGGAGCATTGGATCAAAAAGTTGAGCTTGATGTGCCGTT
GCGCATCCATCTTTCGGCTTGCCCACACTCGTGTAGCCAACATAGCATCGCCGATATTGGATTACAGGGCGGCGTGATTC
GCAACCCTGATGGCAGCAAAAATAAGCTACCAGCCACCGATATTCTGCTCGGCGGAGCTTTGGGCGACGATGCAACAATT
GGCCGACGGGTGGCAACCAAAGTGCCATGGTCAGAATTGGCCGATCGTTTGGGCAACTTGGTCACCACCTACCAAGCCCA
ACGTACCAGCCCTGAAGATCAGTTCCGCCATTGGGCCAAGCGCCAAACCGACGACCAACTGCGGGTTTATCTTGGCTTTG
GCGAGCCAGAAACCGAAGAAGGCGTGTGGACGGAAGAAGGCTCGTGGAATAAAAGTCGCTAG

Upstream 100 bases:

>100_bases
CAAGCCACGCTGAATCGCTCGATGCTAGCGTTGCCCGCATTGTCAACACATTGGAAGAACTCGGCTATCTACCGACGGTT
GCCGCCGTCGAGGAGGGCGC

Downstream 100 bases:

>100_bases
CAGATTCCCTCACCCCCAACCCCTCTCCCACTGCGGCGGGAGAGGGGAGTTTACAAGCGTAGGTCGCTACCCTTTTACCA
ATGATCTGGTAGTTCATCGG

Product: ferredoxin--nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 552

Protein sequence:

>553_residues
MTATTPKLNPVELQKLEKDGLAVLEDIYRYAQSGDINEVTESDMNRFKWYGLYHDKPKDGFFMLRVRLPGGIMTADQADA
IVHLAETVAPGRVEITTRQTFQLHTIALRDIPTVFETLESVGLSSIEACGDVPRNVVSSPVAGIAADEWIDPRPFFKALD
EHFAYNTDYSNLPRKYKVSVAGGGLDATQAPINDLSFTPGRKEIDGEIVLGYNVWVGGGLSHEPHLAQNIDVFVPADLDA
VVEIARAITLVYRDFGYREKRNHARLKYLIADWGAERFREEVVNYLGHPLERAATDVPPAVYSGDVVGVFRQKQPNLYWV
GLLVPTGRITPAQIREAARLSREYGQSEIRLTHSQNLLLPYIPEARLDALLAEPLLQELQPNGPRIQRTVVACTGLPYCN
FATIETKEAAWQLAGALDQKVELDVPLRIHLSACPHSCSQHSIADIGLQGGVIRNPDGSKNKLPATDILLGGALGDDATI
GRRVATKVPWSELADRLGNLVTTYQAQRTSPEDQFRHWAKRQTDDQLRVYLGFGEPETEEGVWTEEGSWNKSR

Sequences:

>Translated_553_residues
MTATTPKLNPVELQKLEKDGLAVLEDIYRYAQSGDINEVTESDMNRFKWYGLYHDKPKDGFFMLRVRLPGGIMTADQADA
IVHLAETVAPGRVEITTRQTFQLHTIALRDIPTVFETLESVGLSSIEACGDVPRNVVSSPVAGIAADEWIDPRPFFKALD
EHFAYNTDYSNLPRKYKVSVAGGGLDATQAPINDLSFTPGRKEIDGEIVLGYNVWVGGGLSHEPHLAQNIDVFVPADLDA
VVEIARAITLVYRDFGYREKRNHARLKYLIADWGAERFREEVVNYLGHPLERAATDVPPAVYSGDVVGVFRQKQPNLYWV
GLLVPTGRITPAQIREAARLSREYGQSEIRLTHSQNLLLPYIPEARLDALLAEPLLQELQPNGPRIQRTVVACTGLPYCN
FATIETKEAAWQLAGALDQKVELDVPLRIHLSACPHSCSQHSIADIGLQGGVIRNPDGSKNKLPATDILLGGALGDDATI
GRRVATKVPWSELADRLGNLVTTYQAQRTSPEDQFRHWAKRQTDDQLRVYLGFGEPETEEGVWTEEGSWNKSR
>Mature_552_residues
TATTPKLNPVELQKLEKDGLAVLEDIYRYAQSGDINEVTESDMNRFKWYGLYHDKPKDGFFMLRVRLPGGIMTADQADAI
VHLAETVAPGRVEITTRQTFQLHTIALRDIPTVFETLESVGLSSIEACGDVPRNVVSSPVAGIAADEWIDPRPFFKALDE
HFAYNTDYSNLPRKYKVSVAGGGLDATQAPINDLSFTPGRKEIDGEIVLGYNVWVGGGLSHEPHLAQNIDVFVPADLDAV
VEIARAITLVYRDFGYREKRNHARLKYLIADWGAERFREEVVNYLGHPLERAATDVPPAVYSGDVVGVFRQKQPNLYWVG
LLVPTGRITPAQIREAARLSREYGQSEIRLTHSQNLLLPYIPEARLDALLAEPLLQELQPNGPRIQRTVVACTGLPYCNF
ATIETKEAAWQLAGALDQKVELDVPLRIHLSACPHSCSQHSIADIGLQGGVIRNPDGSKNKLPATDILLGGALGDDATIG
RRVATKVPWSELADRLGNLVTTYQAQRTSPEDQFRHWAKRQTDDQLRVYLGFGEPETEEGVWTEEGSWNKSR

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=428, Percent_Identity=28.2710280373832, Blast_Score=133, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322597, Length=598, Percent_Identity=26.4214046822742, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 61468; Mature: 61337

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATTPKLNPVELQKLEKDGLAVLEDIYRYAQSGDINEVTESDMNRFKWYGLYHDKPKDG
CCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEECCCCCCC
FFMLRVRLPGGIMTADQADAIVHLAETVAPGRVEITTRQTFQLHTIALRDIPTVFETLES
EEEEEEECCCCEEECCCHHHHHHHHHHCCCCEEEEEECCEEEEEEEEHHHHHHHHHHHHH
VGLSSIEACGDVPRNVVSSPVAGIAADEWIDPRPFFKALDEHFAYNTDYSNLPRKYKVSV
CCCHHHHHHHCCHHHHHCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEE
AGGGLDATQAPINDLSFTPGRKEIDGEIVLGYNVWVGGGLSHEPHLAQNIDVFVPADLDA
ECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEECCCHHH
VVEIARAITLVYRDFGYREKRNHARLKYLIADWGAERFREEVVNYLGHPLERAATDVPPA
HHHHHHHHHHHHHHCCCHHCCCCCCEEEEHHHHCHHHHHHHHHHHHCCCHHHHHCCCCCC
VYSGDVVGVFRQKQPNLYWVGLLVPTGRITPAQIREAARLSREYGQSEIRLTHSQNLLLP
CCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEC
YIPEARLDALLAEPLLQELQPNGPRIQRTVVACTGLPYCNFATIETKEAAWQLAGALDQK
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCC
VELDVPLRIHLSACPHSCSQHSIADIGLQGGVIRNPDGSKNKLPATDILLGGALGDDATI
EEECCCEEEEEECCCCCCCCCCHHHCCCCCCEEECCCCCCCCCCHHHEEEECCCCCCCHH
GRRVATKVPWSELADRLGNLVTTYQAQRTSPEDQFRHWAKRQTDDQLRVYLGFGEPETEE
CCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCC
GVWTEEGSWNKSR
CCCCCCCCCCCCC
>Mature Secondary Structure 
TATTPKLNPVELQKLEKDGLAVLEDIYRYAQSGDINEVTESDMNRFKWYGLYHDKPKDG
CCCCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEECCCCCCC
FFMLRVRLPGGIMTADQADAIVHLAETVAPGRVEITTRQTFQLHTIALRDIPTVFETLES
EEEEEEECCCCEEECCCHHHHHHHHHHCCCCEEEEEECCEEEEEEEEHHHHHHHHHHHHH
VGLSSIEACGDVPRNVVSSPVAGIAADEWIDPRPFFKALDEHFAYNTDYSNLPRKYKVSV
CCCHHHHHHHCCHHHHHCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEE
AGGGLDATQAPINDLSFTPGRKEIDGEIVLGYNVWVGGGLSHEPHLAQNIDVFVPADLDA
ECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEECCCHHH
VVEIARAITLVYRDFGYREKRNHARLKYLIADWGAERFREEVVNYLGHPLERAATDVPPA
HHHHHHHHHHHHHHCCCHHCCCCCCEEEEHHHHCHHHHHHHHHHHHCCCHHHHHCCCCCC
VYSGDVVGVFRQKQPNLYWVGLLVPTGRITPAQIREAARLSREYGQSEIRLTHSQNLLLP
CCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEC
YIPEARLDALLAEPLLQELQPNGPRIQRTVVACTGLPYCNFATIETKEAAWQLAGALDQK
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCC
VELDVPLRIHLSACPHSCSQHSIADIGLQGGVIRNPDGSKNKLPATDILLGGALGDDATI
EEECCCEEEEEECCCCCCCCCCHHHCCCCCCEEECCCCCCCCCCHHHEEEECCCCCCCHH
GRRVATKVPWSELADRLGNLVTTYQAQRTSPEDQFRHWAKRQTDDQLRVYLGFGEPETEE
CCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCC
GVWTEEGSWNKSR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]