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The map label for this gene is ubiE [H]

Identifier: 159897650

GI number: 159897650

Start: 1283791

End: 1284672

Strand: Direct

Name: ubiE [H]

Synonym: Haur_1121

Alternate gene names: 159897650

Gene position: 1283791-1284672 (Clockwise)

Preceding gene: 159897649

Following gene: 159897651

Centisome position: 20.23

GC content: 50.79

Gene sequence:

>882_bases
ATGAACATGACCGAACAACCCTATGTCCCGCTAGCCCCACGCGATGCATCCCTGATGGATACGATGTTGCGCAACGAGGC
TGATCCTGCCTATAGCCGCCGCGCCCGCCGCTTGATGCAATATCTTGATTTGCAGGATGGCGATACGGTACTGGATTGTG
GCTGTGGCTATGGCTTCTATACCCAGATGATCGATAAATTGCGTGATTTGCATGTTGTGGGCTTTGATGAAGCCTTTGAT
CGGCTCCTAGTTGGGCAAGAACATGCCAATCGCGCAACCTTTGTACGTGGCGATGGCCAAGGCATGCCCTTTGCCAATAA
TAGCTTCGATAAAATTCTGCTCTCAGAAGTGCTCGAACATATTCCCGATCAACAGGCAGCCTTGCACGAATTGCGCCGCA
TCCTTAAGCCTGGTGGCATCTTGGCCATTTCAGTGCCGCATGCCAATTATCCGCTATGGTGGGACCCCGCCAGCTGGATC
TGGACGCGGCTTGGTGGTAAGCCCTTCACCCACACCAAATTTGGTAGCATTTGGGAAAACCATGTGCGCTTGTATGAGCC
AGCCGAATTGGTACGTCAAGTGCGTATGGCCGATTTCGAGGTTGAATGCTGCGAAGAAGCCACCCACTACGCTATTCCGA
TGACTCCTTATGTGGTGTATGGCATTGGCAAGCCTTTAATTGAATCGGGTATCTTGCCCGAAAGCTGGATGAAAAGCGCC
GACCGTATGCGAGGCGACCAAAACCAAGGCAGCAAATTGAATCCGCTCAATCTTGCACGCGGCATTATCAATTATGTTGA
TCAACTGAATGAACGCCCAGAAACTTTAGATAAACGCAGCTTTGTGAATGTCTTTGTCAAAGCCCGCAAGCCCTTGAGTT
AA

Upstream 100 bases:

>100_bases
ACAAGTCTTTTCAGTCAAAACCATTCTCGATAGCTACGAGCGGACGATGGCCGAAATGTGTGGTCAGCCCGTTTCTGCCT
CGGTTGTATAAGGAAGAGCG

Downstream 100 bases:

>100_bases
GTCTGTCAGGAGATCGTGTCCATGCTACGAGGTGTCTTCCCAAGCCAGCGCCTAGGCTGGCTTTGGCTTTTAGGCTTGCT
TGGAACAGCCCTAGCGCTCA

Product: type 11 methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MNMTEQPYVPLAPRDASLMDTMLRNEADPAYSRRARRLMQYLDLQDGDTVLDCGCGYGFYTQMIDKLRDLHVVGFDEAFD
RLLVGQEHANRATFVRGDGQGMPFANNSFDKILLSEVLEHIPDQQAALHELRRILKPGGILAISVPHANYPLWWDPASWI
WTRLGGKPFTHTKFGSIWENHVRLYEPAELVRQVRMADFEVECCEEATHYAIPMTPYVVYGIGKPLIESGILPESWMKSA
DRMRGDQNQGSKLNPLNLARGIINYVDQLNERPETLDKRSFVNVFVKARKPLS

Sequences:

>Translated_293_residues
MNMTEQPYVPLAPRDASLMDTMLRNEADPAYSRRARRLMQYLDLQDGDTVLDCGCGYGFYTQMIDKLRDLHVVGFDEAFD
RLLVGQEHANRATFVRGDGQGMPFANNSFDKILLSEVLEHIPDQQAALHELRRILKPGGILAISVPHANYPLWWDPASWI
WTRLGGKPFTHTKFGSIWENHVRLYEPAELVRQVRMADFEVECCEEATHYAIPMTPYVVYGIGKPLIESGILPESWMKSA
DRMRGDQNQGSKLNPLNLARGIINYVDQLNERPETLDKRSFVNVFVKARKPLS
>Mature_293_residues
MNMTEQPYVPLAPRDASLMDTMLRNEADPAYSRRARRLMQYLDLQDGDTVLDCGCGYGFYTQMIDKLRDLHVVGFDEAFD
RLLVGQEHANRATFVRGDGQGMPFANNSFDKILLSEVLEHIPDQQAALHELRRILKPGGILAISVPHANYPLWWDPASWI
WTRLGGKPFTHTKFGSIWENHVRLYEPAELVRQVRMADFEVECCEEATHYAIPMTPYVVYGIGKPLIESGILPESWMKSA
DRMRGDQNQGSKLNPLNLARGIINYVDQLNERPETLDKRSFVNVFVKARKPLS

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323635, Length=118, Percent_Identity=29.6610169491525, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 33482; Mature: 33482

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMTEQPYVPLAPRDASLMDTMLRNEADPAYSRRARRLMQYLDLQDGDTVLDCGCGYGFY
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHH
TQMIDKLRDLHVVGFDEAFDRLLVGQEHANRATFVRGDGQGMPFANNSFDKILLSEVLEH
HHHHHHHHHCEEECHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
IPDQQAALHELRRILKPGGILAISVPHANYPLWWDPASWIWTRLGGKPFTHTKFGSIWEN
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCHHHHHHHCCCCCCCCCHHHHHHHH
HVRLYEPAELVRQVRMADFEVECCEEATHYAIPMTPYVVYGIGKPLIESGILPESWMKSA
HHEEECHHHHHHHHHHHCCHHHHHHHCCCEECCCCCCCEEECCHHHHHCCCCCHHHHHHH
DRMRGDQNQGSKLNPLNLARGIINYVDQLNERPETLDKRSFVNVFVKARKPLS
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNMTEQPYVPLAPRDASLMDTMLRNEADPAYSRRARRLMQYLDLQDGDTVLDCGCGYGFY
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHH
TQMIDKLRDLHVVGFDEAFDRLLVGQEHANRATFVRGDGQGMPFANNSFDKILLSEVLEH
HHHHHHHHHCEEECHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
IPDQQAALHELRRILKPGGILAISVPHANYPLWWDPASWIWTRLGGKPFTHTKFGSIWEN
CCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCHHHHHHHCCCCCCCCCHHHHHHHH
HVRLYEPAELVRQVRMADFEVECCEEATHYAIPMTPYVVYGIGKPLIESGILPESWMKSA
HHEEECHHHHHHHHHHHCCHHHHHHHCCCEECCCCCCCEEECCHHHHHCCCCCHHHHHHH
DRMRGDQNQGSKLNPLNLARGIINYVDQLNERPETLDKRSFVNVFVKARKPLS
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA