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The map label for this gene is mdeA [H]

Identifier: 159897456

GI number: 159897456

Start: 1061343

End: 1062623

Strand: Direct

Name: mdeA [H]

Synonym: Haur_0927

Alternate gene names: 159897456

Gene position: 1061343-1062623 (Clockwise)

Preceding gene: 159897455

Following gene: 159897457

Centisome position: 16.72

GC content: 51.52

Gene sequence:

>1281_bases
ATGGCTGACGAAGTGAAATTCAACCGATTCGAAACCTTAGCGCTCCATGGTGGTCAAAAACCCGATCCAACCACTGGTTC
GCGGGCTGTGCCAATTTACCAAACCACCTCGTATGCCTTTCGGGATACCGACCATGCTGCGGCGTTGTTCTCATTGGCCG
AGCCTGGTAACATTTATACGCGGATTATGAACCCCACCAATGATGTGTTTGAGCAACGCTTGGCTTTGCTCGAAGGTGGG
ATTGGCGCTCTGGCACTGGCATCAGGCCAAGCCGCCGAAACCTTTGCAATTTTGAATGTCGCTGAAGCTGGCGATAACAT
TGTTTCAGCAACCAACTTGTATGGTGGCACTTACAACTTGTTCCACCACACCTTACCACGTTGGGGCGTGCAAACCCGCT
TTGTTGATGCTAGCGATCCCGAAAACTTCCGCGCTGCGGTTGACGAACGCACCAAAGCTTTCTACTTGGAAAGCATTGGC
AATCCTAAGTTGCAAATCCCTGATTTTGAAAAGATTGCCCAAATTGCCCATGAAGTTGGCGTGCCGTTGATTGTCGATAA
CACGGCTGCAACCCCATTCTTGTTGCGGCCAATCGAGCACGGCGCAGACATCGTTGTGCACTCAGCAACCAAATTTATTG
GTGGCCACGGCACGACGATTGGTGGGGTGATTGTTGATGCAGGCAAGTTCGATTGGGCTGCCAGCGGTCGCTTTACCCAA
TACACCACGCCTGATCAATCGTATCACGGCTTGCGCTTTGCTGATTTGGGCGCACCAGCCTACATTCTCAAAGCTCGGGT
GCAATTGTTGCGCGACTTTGGTTCATGTCTCAGCCCATTCAACTCGTTCTTGCTGTTGCAAGGCTTGGAAACTCTGCACT
TGCGCTTGGAACGCCACGTGCAAAACGCTGCGGCTGTTGCTAAGTTCTTAACTGAACATAAGAATGTCGCATGGGTCAAC
TACCCAGGCTTGCCCGGCCACCCATCATATGAATTGGCGCAAAAGTATCTGCCTAAGGGTGCTGGTGCATTGGTTGGCTT
CGGGCTTAAAGGTGGTTTGGCTGCTGGTCGGAGCTTTATCGACAAGTTGCAATTGTTCTCACACTTGGCCAACATTGGCG
ACTCGAAGTCGTTGGCAATTCACCCAGCGACTACGACCCACCAACAATTGACCGATGAAGAACGCTTGGCAACTGGTGTG
ACCGCCGATTACGTTCGCTTGTCAGTGGGTACTGAAGCCATCGACGATATTCTCGCCGATTTGGAACAAGCCCTTGCTTA
A

Upstream 100 bases:

>100_bases
TGATGAGAGGTTTTTTGGTTCGTAGGCACTGAAGCCTATCGCAATGGCGCGAATCGGAACAACCGTAGCATTCGAAGATT
ATAAACAAGGAGTCCTGATC

Downstream 100 bases:

>100_bases
TCGCTTAGTGTAGTGGTTTACGGGATTGTTGCTCATGCCCTCACCCCCTCTCCCGCCGAGCGAGGCGAGGAACCACCAAA
CCGGACTCCCCTCGCCCGCT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MADEVKFNRFETLALHGGQKPDPTTGSRAVPIYQTTSYAFRDTDHAAALFSLAEPGNIYTRIMNPTNDVFEQRLALLEGG
IGALALASGQAAETFAILNVAEAGDNIVSATNLYGGTYNLFHHTLPRWGVQTRFVDASDPENFRAAVDERTKAFYLESIG
NPKLQIPDFEKIAQIAHEVGVPLIVDNTAATPFLLRPIEHGADIVVHSATKFIGGHGTTIGGVIVDAGKFDWAASGRFTQ
YTTPDQSYHGLRFADLGAPAYILKARVQLLRDFGSCLSPFNSFLLLQGLETLHLRLERHVQNAAAVAKFLTEHKNVAWVN
YPGLPGHPSYELAQKYLPKGAGALVGFGLKGGLAAGRSFIDKLQLFSHLANIGDSKSLAIHPATTTHQQLTDEERLATGV
TADYVRLSVGTEAIDDILADLEQALA

Sequences:

>Translated_426_residues
MADEVKFNRFETLALHGGQKPDPTTGSRAVPIYQTTSYAFRDTDHAAALFSLAEPGNIYTRIMNPTNDVFEQRLALLEGG
IGALALASGQAAETFAILNVAEAGDNIVSATNLYGGTYNLFHHTLPRWGVQTRFVDASDPENFRAAVDERTKAFYLESIG
NPKLQIPDFEKIAQIAHEVGVPLIVDNTAATPFLLRPIEHGADIVVHSATKFIGGHGTTIGGVIVDAGKFDWAASGRFTQ
YTTPDQSYHGLRFADLGAPAYILKARVQLLRDFGSCLSPFNSFLLLQGLETLHLRLERHVQNAAAVAKFLTEHKNVAWVN
YPGLPGHPSYELAQKYLPKGAGALVGFGLKGGLAAGRSFIDKLQLFSHLANIGDSKSLAIHPATTTHQQLTDEERLATGV
TADYVRLSVGTEAIDDILADLEQALA
>Mature_425_residues
ADEVKFNRFETLALHGGQKPDPTTGSRAVPIYQTTSYAFRDTDHAAALFSLAEPGNIYTRIMNPTNDVFEQRLALLEGGI
GALALASGQAAETFAILNVAEAGDNIVSATNLYGGTYNLFHHTLPRWGVQTRFVDASDPENFRAAVDERTKAFYLESIGN
PKLQIPDFEKIAQIAHEVGVPLIVDNTAATPFLLRPIEHGADIVVHSATKFIGGHGTTIGGVIVDAGKFDWAASGRFTQY
TTPDQSYHGLRFADLGAPAYILKARVQLLRDFGSCLSPFNSFLLLQGLETLHLRLERHVQNAAAVAKFLTEHKNVAWVNY
PGLPGHPSYELAQKYLPKGAGALVGFGLKGGLAAGRSFIDKLQLFSHLANIGDSKSLAIHPATTTHQQLTDEERLATGVT
ADYVRLSVGTEAIDDILADLEQALA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=427, Percent_Identity=35.3629976580796, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI299473759, Length=427, Percent_Identity=32.0843091334895, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI25453487, Length=426, Percent_Identity=31.924882629108, Blast_Score=168, Evalue=8e-42,
Organism=Escherichia coli, GI1790375, Length=412, Percent_Identity=32.7669902912621, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1789383, Length=438, Percent_Identity=26.027397260274, Blast_Score=122, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=35.2245862884161, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=34.9056603773585, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI71980708, Length=428, Percent_Identity=31.0747663551402, Blast_Score=204, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=52.4249422632794, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6319307, Length=425, Percent_Identity=34.3529411764706, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=442, Percent_Identity=25.5656108597285, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=347, Percent_Identity=26.5129682997118, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=434, Percent_Identity=25.5760368663594, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19922554, Length=430, Percent_Identity=32.5581395348837, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46048; Mature: 45917

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEVKFNRFETLALHGGQKPDPTTGSRAVPIYQTTSYAFRDTDHAAALFSLAEPGNIYT
CCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHCCCCCEEE
RIMNPTNDVFEQRLALLEGGIGALALASGQAAETFAILNVAEAGDNIVSATNLYGGTYNL
EEECCHHHHHHHHHHHHHCCCCEEEECCCCCHHHEEEEEHHHCCCCEEEEHHCCCCCHHH
FHHTLPRWGVQTRFVDASDPENFRAAVDERTKAFYLESIGNPKLQIPDFEKIAQIAHEVG
HHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCC
VPLIVDNTAATPFLLRPIEHGADIVVHSATKFIGGHGTTIGGVIVDAGKFDWAASGRFTQ
CCEEECCCCCCCCEEEHHCCCCCEEEECHHHHHCCCCCEECEEEEECCCCCEECCCCEEE
YTTPDQSYHGLRFADLGAPAYILKARVQLLRDFGSCLSPFNSFLLLQGLETLHLRLERHV
ECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNAAAVAKFLTEHKNVAWVNYPGLPGHPSYELAQKYLPKGAGALVGFGLKGGLAAGRSFI
HHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
DKLQLFSHLANIGDSKSLAIHPATTTHQQLTDEERLATGVTADYVRLSVGTEAIDDILAD
HHHHHHHHHHHCCCCCCEEEECCCCCHHHHCCHHHHHCCCCHHHEEEECCHHHHHHHHHH
LEQALA
HHHHCC
>Mature Secondary Structure 
ADEVKFNRFETLALHGGQKPDPTTGSRAVPIYQTTSYAFRDTDHAAALFSLAEPGNIYT
CCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHCCCCCEEE
RIMNPTNDVFEQRLALLEGGIGALALASGQAAETFAILNVAEAGDNIVSATNLYGGTYNL
EEECCHHHHHHHHHHHHHCCCCEEEECCCCCHHHEEEEEHHHCCCCEEEEHHCCCCCHHH
FHHTLPRWGVQTRFVDASDPENFRAAVDERTKAFYLESIGNPKLQIPDFEKIAQIAHEVG
HHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCC
VPLIVDNTAATPFLLRPIEHGADIVVHSATKFIGGHGTTIGGVIVDAGKFDWAASGRFTQ
CCEEECCCCCCCCEEEHHCCCCCEEEECHHHHHCCCCCEECEEEEECCCCCEECCCCEEE
YTTPDQSYHGLRFADLGAPAYILKARVQLLRDFGSCLSPFNSFLLLQGLETLHLRLERHV
ECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QNAAAVAKFLTEHKNVAWVNYPGLPGHPSYELAQKYLPKGAGALVGFGLKGGLAAGRSFI
HHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHH
DKLQLFSHLANIGDSKSLAIHPATTTHQQLTDEERLATGVTADYVRLSVGTEAIDDILAD
HHHHHHHHHHHCCCCCCEEEECCCCCHHHHCCHHHHHCCCCHHHEEEECCHHHHHHHHHH
LEQALA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]