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The map label for this gene is afr [H]

Identifier: 159897266

GI number: 159897266

Start: 844769

End: 845809

Strand: Direct

Name: afr [H]

Synonym: Haur_0737

Alternate gene names: 159897266

Gene position: 844769-845809 (Clockwise)

Preceding gene: 159897265

Following gene: 159897267

Centisome position: 13.31

GC content: 50.14

Gene sequence:

>1041_bases
ATGAAAACAATCAGGTTAGGAGTGCTTGGAGCGGGTTTGGCGGTCAAACATTTGCATCTACCAGCGCTCAAAGCATTACC
AAATTGCTTTGAAATTGTTAGCATTTGCGATCATAATTGGGCCAATGCTGAGGCGCTGGCAGGCTTGCTCGATCTCCACC
CAACGATCACTGATTCGTGGGTGGATTTCTTTGCCGATTCCCAAATCGAGGCGGTATTAATTTCGTTGCCAATTCAGCGT
AATGCCGAGGCAATTCAAGCAGCGGTCGCGGCAGGCAAACATGTAATTTGCGAAAAACCCTTGGCCGCCAATTTGCCGCA
GGCCGAAGCGTTGGTCGCAGCCTGTCAACATGCTCCAGTCAAAATTTTGATCGCCGAAAATTTTCACTATCGCGATGATC
TCAAACAAGCCCGCGCTTGGATGGATGCTGGGGCAATTGGCGATTTAGTATTAATTGAACTTCGCGCCACCTTCTGGAGC
GATCCCGATCAAGGCTTTGGGGCAACGCCTTGGCGACATGATCATCAATATCGTGGCGCAGTCTTGGCCGATGCCGGGGT
GCATCAAGCTGCTTTATTGCGCGAACTAGGCGGGCCAATCGAAAGTGCCCATGCTTTCATTAAGGATGTGCATCCCGTGC
TCCGTGGCCCAGACACGATGGTGCTGAATCTGCGTTTTGCCTCAGGCGTGCTTGGTTCGTTGTGTTTTTCGGGCGCAGTG
AAAACCGAAAATCCCTGTTTTGATTGGACGGTCGTGCATGGAACCCACGGTAGTATTACCATCCGTGGCGATCAGACGAC
ATTATATCGGGCTGATGGTACAATAGAGCACTATCAAGCTCAAGATTTGCGTGGCTATATCAGCGAGTTTCGCAATTTTT
ATGCGGCAATTGTTGAAAACGAGCCAATTGTGGCTAGTTTGGCTACTGCGCTGGCCGATTGGCGTTTGATCATGCGGGCC
ATCGATGCTGCTGAAAGTAATAGTGTGCAGCAAATCGACCCAATTGTGCCCTTGGCAAGCTTGAATGATCTTCCGAACTA
A

Upstream 100 bases:

>100_bases
TTGAAGCTGATGATCCATTAACCCCACCATCGCCCTTTAAAATTCGCCGTCGCCCAACCCTGATGCAACGTCGGCGTTTC
CAGCGTGAGGTCCGCCATTA

Downstream 100 bases:

>100_bases
AACAAGGATTTCGATACAATGACTGAACAATGGCCTGATGATGCCGAACAAATTGGCAATTTAGTATTTGTGCCCAACCG
CGACTACCCCTATCCTTTTA

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKTIRLGVLGAGLAVKHLHLPALKALPNCFEIVSICDHNWANAEALAGLLDLHPTITDSWVDFFADSQIEAVLISLPIQR
NAEAIQAAVAAGKHVICEKPLAANLPQAEALVAACQHAPVKILIAENFHYRDDLKQARAWMDAGAIGDLVLIELRATFWS
DPDQGFGATPWRHDHQYRGAVLADAGVHQAALLRELGGPIESAHAFIKDVHPVLRGPDTMVLNLRFASGVLGSLCFSGAV
KTENPCFDWTVVHGTHGSITIRGDQTTLYRADGTIEHYQAQDLRGYISEFRNFYAAIVENEPIVASLATALADWRLIMRA
IDAAESNSVQQIDPIVPLASLNDLPN

Sequences:

>Translated_346_residues
MKTIRLGVLGAGLAVKHLHLPALKALPNCFEIVSICDHNWANAEALAGLLDLHPTITDSWVDFFADSQIEAVLISLPIQR
NAEAIQAAVAAGKHVICEKPLAANLPQAEALVAACQHAPVKILIAENFHYRDDLKQARAWMDAGAIGDLVLIELRATFWS
DPDQGFGATPWRHDHQYRGAVLADAGVHQAALLRELGGPIESAHAFIKDVHPVLRGPDTMVLNLRFASGVLGSLCFSGAV
KTENPCFDWTVVHGTHGSITIRGDQTTLYRADGTIEHYQAQDLRGYISEFRNFYAAIVENEPIVASLATALADWRLIMRA
IDAAESNSVQQIDPIVPLASLNDLPN
>Mature_346_residues
MKTIRLGVLGAGLAVKHLHLPALKALPNCFEIVSICDHNWANAEALAGLLDLHPTITDSWVDFFADSQIEAVLISLPIQR
NAEAIQAAVAAGKHVICEKPLAANLPQAEALVAACQHAPVKILIAENFHYRDDLKQARAWMDAGAIGDLVLIELRATFWS
DPDQGFGATPWRHDHQYRGAVLADAGVHQAALLRELGGPIESAHAFIKDVHPVLRGPDTMVLNLRFASGVLGSLCFSGAV
KTENPCFDWTVVHGTHGSITIRGDQTTLYRADGTIEHYQAQDLRGYISEFRNFYAAIVENEPIVASLATALADWRLIMRA
IDAAESNSVQQIDPIVPLASLNDLPN

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol [H]

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI87082405, Length=255, Percent_Identity=24.3137254901961, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323975, Length=316, Percent_Identity=27.2151898734177, Blast_Score=119, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 37572; Mature: 37572

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIRLGVLGAGLAVKHLHLPALKALPNCFEIVSICDHNWANAEALAGLLDLHPTITDSW
CCEEEEEEEECCHHHHHHCCHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHH
VDFFADSQIEAVLISLPIQRNAEAIQAAVAAGKHVICEKPLAANLPQAEALVAACQHAPV
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHCCCCHHHHHHHHHCCCCE
KILIAENFHYRDDLKQARAWMDAGAIGDLVLIELRATFWSDPDQGFGATPWRHDHQYRGA
EEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCE
VLADAGVHQAALLRELGGPIESAHAFIKDVHPVLRGPDTMVLNLRFASGVLGSLCFSGAV
EEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCC
KTENPCFDWTVVHGTHGSITIRGDQTTLYRADGTIEHYQAQDLRGYISEFRNFYAAIVEN
CCCCCCEEEEEEECCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
EPIVASLATALADWRLIMRAIDAAESNSVQQIDPIVPLASLNDLPN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHCCCCC
>Mature Secondary Structure
MKTIRLGVLGAGLAVKHLHLPALKALPNCFEIVSICDHNWANAEALAGLLDLHPTITDSW
CCEEEEEEEECCHHHHHHCCHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHH
VDFFADSQIEAVLISLPIQRNAEAIQAAVAAGKHVICEKPLAANLPQAEALVAACQHAPV
HHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEECCCHHCCCCHHHHHHHHHCCCCE
KILIAENFHYRDDLKQARAWMDAGAIGDLVLIELRATFWSDPDQGFGATPWRHDHQYRGA
EEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCE
VLADAGVHQAALLRELGGPIESAHAFIKDVHPVLRGPDTMVLNLRFASGVLGSLCFSGAV
EEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCC
KTENPCFDWTVVHGTHGSITIRGDQTTLYRADGTIEHYQAQDLRGYISEFRNFYAAIVEN
CCCCCCEEEEEEECCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
EPIVASLATALADWRLIMRAIDAAESNSVQQIDPIVPLASLNDLPN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]