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The map label for this gene is truB [H]

Identifier: 159897011

GI number: 159897011

Start: 557966

End: 558841

Strand: Direct

Name: truB [H]

Synonym: Haur_0479

Alternate gene names: 159897011

Gene position: 557966-558841 (Clockwise)

Preceding gene: 159897010

Following gene: 159897013

Centisome position: 8.79

GC content: 53.2

Gene sequence:

>876_bases
ATGCTGCATGGATTTTTAAACATTGATAAACCTCAAGGCATAACCTCGACCGATGTGGTGCGGGTGGTCAAACGTAATGC
GCGTCAAAAACGCGTTGGTCATGGCGGCACGCTTGATCCCATGGCAACGGGCGTGCTGCCGATTGCTTTGGGCAACGCCA
CGCGCTTGCTCGAATATCTGCTTGAGGAAGAGCGAAAAGCCTATACTGCAACCCTTCGACTGGGCATTACCACTGATAGC
GATGATGTTGAGGGCGCAGTGATCGCCGAAGCACCGATTCCGCCACTTGATCCAGCCTTGATTGAAAGCGTGCTCAGCCA
ATTTCGCGGCGCGATCAACCAAGTTCCGCCGCAATATGCAGCAATTCGCGTCGATGGTAAGCGCATGTATGAATATGCCC
GCGAAGGCAAGCATATCGAGTTGCCCGCCCGCCCAATCACCATTGAGCAACTTGATCTCTTGGCATGGGATGCCCAGCAA
TTAACGATTGCCGTTGATTGCAGCAAAGGCACCTATATTCGAGCGATTGCCCGTGATATTGGGGCATTGCTCGGTTGTGG
TGCGCATTTAACTGCCTTACGCCGTACCCGCGCTGGAGCCTTTGATCTGAGCAACAGCATTGGCTTGGCCGAGCTTGATC
AACAACCTGAGGTTTTTGCCGCAGCTTTATTGCCACCGCAGGCAGCAATTGCCAACTGGCCATTAATTAATTTGGCTGAT
GCGATTGTGGCGGATGTGCGCATGGGTCGAGTTGTGCAGGTTGATAGCCAAGCCGAACGGGTTGGGTTGCTCGATCAAGC
AGGCCAATTGGTAGCGATTGCCGTATTGCGTGAAGCTGGCTATCAGCCAATCAAGGTCTTTGCCGCCGAGGAATAA

Upstream 100 bases:

>100_bases
GGCCGAGGCTCGCGATTTGGTGTTAGGTGAATTACGGGCTGCCTTAGCCTAAATCAATAGAAAGGTTGTTGGCTCGTTAA
TCAACGGGGCATTGTTAGCT

Downstream 100 bases:

>100_bases
AAGCTGAGGCTATGGGCTTTTGGCGCTAGGGATTTATGGTCTATCCCAAACAAACTTCCGCTTGCCCAGAGCCTTCAGCC
TAGAGCCTTATGCTCTGCTT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLHGFLNIDKPQGITSTDVVRVVKRNARQKRVGHGGTLDPMATGVLPIALGNATRLLEYLLEEERKAYTATLRLGITTDS
DDVEGAVIAEAPIPPLDPALIESVLSQFRGAINQVPPQYAAIRVDGKRMYEYAREGKHIELPARPITIEQLDLLAWDAQQ
LTIAVDCSKGTYIRAIARDIGALLGCGAHLTALRRTRAGAFDLSNSIGLAELDQQPEVFAAALLPPQAAIANWPLINLAD
AIVADVRMGRVVQVDSQAERVGLLDQAGQLVAIAVLREAGYQPIKVFAAEE

Sequences:

>Translated_291_residues
MLHGFLNIDKPQGITSTDVVRVVKRNARQKRVGHGGTLDPMATGVLPIALGNATRLLEYLLEEERKAYTATLRLGITTDS
DDVEGAVIAEAPIPPLDPALIESVLSQFRGAINQVPPQYAAIRVDGKRMYEYAREGKHIELPARPITIEQLDLLAWDAQQ
LTIAVDCSKGTYIRAIARDIGALLGCGAHLTALRRTRAGAFDLSNSIGLAELDQQPEVFAAALLPPQAAIANWPLINLAD
AIVADVRMGRVVQVDSQAERVGLLDQAGQLVAIAVLREAGYQPIKVFAAEE
>Mature_291_residues
MLHGFLNIDKPQGITSTDVVRVVKRNARQKRVGHGGTLDPMATGVLPIALGNATRLLEYLLEEERKAYTATLRLGITTDS
DDVEGAVIAEAPIPPLDPALIESVLSQFRGAINQVPPQYAAIRVDGKRMYEYAREGKHIELPARPITIEQLDLLAWDAQQ
LTIAVDCSKGTYIRAIARDIGALLGCGAHLTALRRTRAGAFDLSNSIGLAELDQQPEVFAAALLPPQAAIANWPLINLAD
AIVADVRMGRVVQVDSQAERVGLLDQAGQLVAIAVLREAGYQPIKVFAAEE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=219, Percent_Identity=35.1598173515982, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI4503337, Length=199, Percent_Identity=32.6633165829146, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI215599015, Length=199, Percent_Identity=32.6633165829146, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI2367200, Length=248, Percent_Identity=39.1129032258064, Blast_Score=188, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17553978, Length=206, Percent_Identity=29.6116504854369, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=133, Percent_Identity=38.3458646616541, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=206, Percent_Identity=29.6116504854369, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364189, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364187, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364185, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364183, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI62471759, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17975520, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 31394; Mature: 31394

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHGFLNIDKPQGITSTDVVRVVKRNARQKRVGHGGTLDPMATGVLPIALGNATRLLEYL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEECCHHHHHHHH
LEEERKAYTATLRLGITTDSDDVEGAVIAEAPIPPLDPALIESVLSQFRGAINQVPPQYA
HHHHHHHEEEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
AIRVDGKRMYEYAREGKHIELPARPITIEQLDLLAWDAQQLTIAVDCSKGTYIRAIARDI
EEEECHHHHHHHHHCCCEEECCCCCCCHHHHHHEEECCCEEEEEEECCCCCHHHHHHHHH
GALLGCGAHLTALRRTRAGAFDLSNSIGLAELDQQPEVFAAALLPPQAAIANWPLINLAD
HHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCHHHHHCCCHHHHHH
AIVADVRMGRVVQVDSQAERVGLLDQAGQLVAIAVLREAGYQPIKVFAAEE
HHHHHHHCCCEEEECCHHHHHCHHHHCCHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MLHGFLNIDKPQGITSTDVVRVVKRNARQKRVGHGGTLDPMATGVLPIALGNATRLLEYL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEECCHHHHHHHH
LEEERKAYTATLRLGITTDSDDVEGAVIAEAPIPPLDPALIESVLSQFRGAINQVPPQYA
HHHHHHHEEEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEE
AIRVDGKRMYEYAREGKHIELPARPITIEQLDLLAWDAQQLTIAVDCSKGTYIRAIARDI
EEEECHHHHHHHHHCCCEEECCCCCCCHHHHHHEEECCCEEEEEEECCCCCHHHHHHHHH
GALLGCGAHLTALRRTRAGAFDLSNSIGLAELDQQPEVFAAALLPPQAAIANWPLINLAD
HHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCHHHHHCCCHHHHHH
AIVADVRMGRVVQVDSQAERVGLLDQAGQLVAIAVLREAGYQPIKVFAAEE
HHHHHHHCCCEEEECCHHHHHCHHHHCCHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA